Restriction map for
pFN21AE3661
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AflIII AgeI AhdI AlwI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI FbaI FseI HindIII
HpaI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1099 GTCTAC GT^[AC][GT]AC
AcyI 3 GGCGTC G[GA]^CG[CT]C
Alw44I 637 GTGCAC G^TGCAC
AlwNI 550 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 717 CCTTAGG CC^T[ACGT]AGG
ApaI 1026 GGGCCC GGGCC^C
ApaLI 637 GTGCAC G^TGCAC
AvaI 674 CCCGGG C^[CT]CG[GA]G
AviII 608 TGCGCA TGC^GCA
BciVI 460 GTATCC GTATCC(6/5)
BglII 1196 AGATCT A^GATCT
BpmI 787 CTGGAG CTGGAG(16/14)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsoBI 674 CCCGGG C^[CT]CG[GA]G
BspDI 338 ATCGAT AT^CGAT
BsrDI 474 CATTGC GCAATG(2/0)
BstYI 1196 AGATCT [GA]^GATC[CT]
Bsu36I 717 CCTTAGG CC^T[ACGT]AGG
ClaI 338 ATCGAT AT^CGAT
DraII 48 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 128 CGGCCG [CT]^GGCC[GA]
EagI 128 CGGCCG C^GGCCG
EclXI 128 CGGCCG C^GGCCG
Eco52I 128 CGGCCG C^GGCCG
Eco81I 717 CCTTAGG CC^T[ACGT]AGG
EcoO109I 48 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1120 GATATC GAT^ATC
EcoT14I 528 CCATGG C^C[AT][AT]GG
EcoT22I 484 ATGCAT ATGCA^T
FspI 608 TGCGCA TGC^GCA
HaeII 897 AGCGCC [GA]GCGC^[CT]
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 250 GTCAAC GT[CT]^[GA]AC
HindII 250 GTCAAC GT[CT]^[GA]AC
MflI 1196 AGATCT [GA]^GATC[CT]
MslI 597 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 528 CCATGG C^CATGG
NsiI 484 ATGCAT ATGCA^T
PflMI 549 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 441 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 936 CTGCAG CTGCA^G
PvuI 435 CGATCG CGAT^CG
PvuII 823 CAGCTG CAG^CTG
SacI 181 GAGCTC GAGCT^C
SmaI 674 CCCGGG CCC^GGG
SmlI 655 CTCAAG C^T[CT][GA]AG
SspI 780 AATATT AAT^ATT
StyI 528 CCATGG C^C[AT][AT]GG
TfiI 1232 GATTC G^A[AT]TC
Tsp509I 707 AATT ^AATT
Van91I 549 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1196 AGATCT [GA]^GATC[CT]
XmaCI 674 CCCGGG C^CCGGG
XmaI 674 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1067 TCCGGA T^CCGGA
1212 TCCGGA
AfaI 513 GTAC GT^AC
564 GTAC
Asp700I 90 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
114 GAAGGTGTTC
AspHI 181 GAGCTC G[AT]GC[AT]^C
637 GTGCAC
AvaII 15 GGTCC G^G[AT]CC
949 GGACC
BanI 192 GGTGCC G^G[CT][GA]CC
976 GGCACC
BlpI 183 GCTCAGC GC^T[ACGT]AGC
1225 GCTAAGC
Bpu1102I 183 GCTCAGC GC^T[ACGT]AGC
1225 GCTAAGC
BsaWI 1067 TCCGGA [AT]^CCGG[AT]
1212 TCCGGA
BseAI 1067 TCCGGA T^CCGGA
1212 TCCGGA
BsgI 100 GTGCAG GTGCAG(16/14)
820 GTGCAG
BsiEI 128 CGGCCG CG[GA][CT]^CG
435 CGATCG
BsiHKAI 181 GAGCTC G[AT]GC[AT]^C
637 GTGCAC
BspEI 1067 TCCGGA T^CCGGA
1212 TCCGGA
BstAPI 93 GCATTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1153 GCAGCTCATGC
CelII 183 GCTCAGC GC^T[ACGT]AGC
1225 GCTAAGC
CfoI 609 GCGC GCG^C
898 GCGC
DsaI 26 CCACGG C^C[GA][CT]GG
528 CCATGG
EarI 766 GAAGAG CTCTTC(1/4)
798 GAAGAG
FauI 135 CCCGC CCCGC(4/6)
906 GCGGG
HhaI 609 GCGC GCG^C
898 GCGC
HinP1I 609 GCGC G^CGC
898 GCGC
Ksp632I 766 GAAGAG CTCTTC(1/4)
798 GAAGAG
MroI 1067 TCCGGA T^CCGGA
1212 TCCGGA
MseI 227 TTAA T^TAA
701 TTAA
MspA1I 136 CCGCTG C[AC]G^C[GT]G
823 CAGCTG
NspI 482 ACATGC [GA]CATG^[CT]
596 ACATGC
RsaI 513 GTAC GT^AC
564 GTAC
SfcI 840 CTACAG C^T[GA][CT]AG
936 CTGCAG
TaqI 172 TCGA T^CGA
339 TCGA
Tru9I 227 TTAA T^TAA
701 TTAA
TthHB8I 172 TCGA T^CGA
339 TCGA
XmnI 90 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
114 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI