Restriction map for
pFN21AE1576
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BbeI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI KasI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 167 GGTACC G^GTACC
Asp718I 167 GGTACC G^GTACC
BalI 278 TGGCCA TGG^CCA
BamHI 265 GGATCC G^GATCC
BanI 167 GGTACC G^G[CT][GA]CC
BbrPI 425 CACGTG CAC^GTG
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BcnI 181 CCGGG CC^[GC]GG
BsaAI 425 CACGTG [CT]AC^GT[GA]
BseRI 373 GAGGAG GAGGAG(10/8)
Bsp1407I 152 TGTACA T^GTACA
BspMI 118 ACCTGC ACCTGC(4/8)
BsrGI 152 TGTACA T^GTACA
BsrI 164 ACTGG ACTGG(1/-1)
BstXI 269 CCAAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 265 GGATCC [GA]^GATC[CT]
DpnII 266 GATC ^GATC
EcoT14I 96 CCTTGG C^C[AT][AT]GG
FauI 356 CCCGC CCCGC(4/6)
HgaI 158 GCGTC GACGC(5/10)
HphI 116 TCACC GGTGA(8/7)
KpnI 167 GGTACC GGTAC^C
MaeII 426 ACGT A^CGT
MboI 266 GATC ^GATC
MboII 397 GAAGA GAAGA(8/7)
MflI 265 GGATCC [GA]^GATC[CT]
MluNI 278 TGGCCA TGG^CCA
MscI 278 TGGCCA TGG^CCA
MslI 144 CATCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 78 GCCGGC GCC^GGC
NciI 181 CCGGG CC^[GC]GG
NdeII 266 GATC ^GATC
NgoMIV 78 GCCGGC G^CCGGC
PmaCI 425 CACGTG CAC^GTG
PmlI 425 CACGTG CAC^GTG
PpuMI 245 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 423 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 266 GATC ^GATC
SspBI 152 TGTACA T^GTACA
StyI 96 CCTTGG C^C[AT][AT]GG
TspRI 411 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 265 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 34 GTCTAC GT^[AC][GT]AC
160 GTCTAC
AfaI 153 GTAC GT^AC
168 GTAC
AflIII 424 ACACGT A^C[GA][CT]GT
426 ACGTGT
AluI 311 AGCT AG^CT
361 AGCT
AlwI 266 GATCC GGATC(4/5)
265 GGATC
AocI 126 CCTGAGG CC^T[ACGT]AGG
319 CCTGAGG
ApaI 237 GGGCCC GGGCC^C
353 GGGCCC
AvaII 52 GGTCC G^G[AT]CC
246 GGTCC
BsgI 39 CTGCAC GTGCAG(16/14)
120 CTGCAC
BsiYI 281 CCAGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCGCAGCTCGG
BslI 281 CCAGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCGCAGCTCGG
BsmFI 247 GTCCC GGGAC(10/14)
430 GTCCC
BsrFI 60 GCCGGT [GA]^CCGG[CT]
78 GCCGGC
Bsu36I 126 CCTGAGG CC^T[ACGT]AGG
319 CCTGAGG
Cac8I 78 GCCGGC GC[ACGT]^[ACGT]GC
355 GCCCGC
Cfr10I 60 GCCGGT [GA]^CCGG[CT]
78 GCCGGC
EaeI 80 CGGCCA [CT]^GGCC[GA]
278 TGGCCA
Eco81I 126 CCTGAGG CC^T[ACGT]AGG
319 CCTGAGG
PstI 313 CTGCAG CTGCA^G
413 CTGCAG
RsaI 153 GTAC GT^AC
168 GTAC
SfaNI 69 GCATC GCATC(5/9)
302 GCATC
SfcI 313 CTGCAG C^T[GA][CT]AG
413 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BanII BbvI BmyI BsaJI
Bsp1286I BssKI BstNI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII ItaI MnlI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI