Restriction map for
pFN21AA1063
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmeI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 1064 ACGTGT A^C[GA][CT]GT
AgeI 704 ACCGGT A^CCGGT
AlwI 987 GGATC GGATC(4/5)
AvaI 19 CCCGAG C^[CT]CG[GA]G
BbrPI 1063 CACGTG CAC^GTG
BbsI 238 GTCTTC GAAGAC(2/6)
BbvCI 151 GCTGAGG CCTCAGC(-5/-2)
BclI 533 TGATCA T^GATCA
BfaI 763 CTAG C^TAG
BglI 40 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 896 AGATCT A^GATCT
BpuAI 238 GTCTTC GAAGAC(2/6)
BsaWI 704 ACCGGT [AT]^CCGG[AT]
BseRI 388 GAGGAG GAGGAG(10/8)
BsmI 219 GCATTC GAATGC(1/-1)
BsoBI 19 CCCGAG C^[CT]CG[GA]G
BspHI 557 TCATGA T^CATGA
BsrBI 141 GAGCGG CCGCTC(-3/-3)
BsrFI 704 ACCGGT [GA]^CCGG[CT]
BstXI 189 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 704 ACCGGT [GA]^CCGG[CT]
DraIII 736 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 999 CTCTTC CTCTTC(1/4)
FbaI 533 TGATCA T^GATCA
HincII 1405 GTTAAC GT[CT]^[GA]AC
HindII 1405 GTTAAC GT[CT]^[GA]AC
HindIII 401 AAGCTT A^AGCTT
HpaI 1405 GTTAAC GTT^AAC
Ksp632I 999 CTCTTC CTCTTC(1/4)
MaeI 763 CTAG C^TAG
MseI 1406 TTAA T^TAA
PinAI 704 ACCGGT A^CCGGT
PmaCI 1063 CACGTG CAC^GTG
PmlI 1063 CACGTG CAC^GTG
PpuMI 1417 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 701 GACACCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 557 TCATGA T^CATGA
SapI 998 GCTCTTC GCTCTTC(1/4)
SexAI 737 ACCTGGT A^CC[AT]GGT
SphI 276 GCATGC GCATG^C
TfiI 1103 GATTC G^A[AT]TC
Tru9I 1406 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 922 GACGTC GACGT^C
1516 GACGTC
AccII 753 CGCG CG^CG
804 CGCG
AfaI 1347 GTAC GT^AC
1536 GTAC
AspHI 817 GTGCTC G[AT]GC[AT]^C
1117 GAGCAC
BalI 302 TGGCCA TGG^CCA
1431 TGGCCA
BanI 623 GGCACC G^G[CT][GA]CC
758 GGCACC
BpmI 1317 CTCCAG CTGGAG(16/14)
1288 CTGGAG
BsaAI 1063 CACGTG [CT]AC^GT[GA]
1269 CACGTA
BsiHKAI 817 GTGCTC G[AT]GC[AT]^C
1117 GAGCAC
BsmAI 949 GTCTC GTCTC(1/5)
1367 GTCTC
BspMI 110 ACCTGC ACCTGC(4/8)
928 ACCTGC
BssSI 271 CACGAG CACGAG(-5/-1)
790 CACGAG
BstAPI 105 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1148 GCAGCGGTTGC
BstF5I 717 CATCC GGATG(2/0)
1334 GGATG
BstUI 753 CGCG CG^CG
804 CGCG
BstYI 896 AGATCT [GA]^GATC[CT]
987 GGATCT
DraII 575 AGGCCCT [GA]G^G[ACGT]CC[CT]
1417 GGGACCT
EcoO109I 575 AGGCCCT [GA]G^G[ACGT]CC[CT]
1417 GGGACCT
FokI 717 CATCC GGATG(9/13)
1334 GGATG
HaeII 54 AGCGCC [GA]GCGC^[CT]
1132 AGCGCC
MaeIII 925 GTCAC ^GT[ACGT]AC
1481 GTGAC
MflI 896 AGATCT [GA]^GATC[CT]
987 GGATCT
MluNI 302 TGGCCA TGG^CCA
1431 TGGCCA
MscI 302 TGGCCA TGG^CCA
1431 TGGCCA
MspA1I 627 CCGCTG C[AC]G^C[GT]G
1149 CAGCGG
MvnI 753 CGCG CG^CG
804 CGCG
NcoI 32 CCATGG C^CATGG
942 CCATGG
NspI 276 GCATGC [GA]CATG^[CT]
550 ACATGC
PleI 947 GAGTC GAGTC(4/5)
1171 GAGTC
RsaI 1347 GTAC GT^AC
1536 GTAC
TaqI 505 TCGA T^CGA
962 TCGA
Tsp45I 925 GTCAC ^GT[GC]AC
1481 GTGAC
TthHB8I 505 TCGA T^CGA
962 TCGA
XcmI 647 CCAGCTCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
933 CCAAGTCTGCCATGG
XhoII 896 AGATCT [GA]^GATC[CT]
987 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BanII BbvI BcnI BmyI BsaHI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI