Restriction map for
pFN21AB8608
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HpaI KasI KpnI MluI
MroI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 173 GTATAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AcyI 568 GGCGTC G[GA]^CG[CT]C
Alw44I 1443 GTGCAC G^TGCAC
AocI 475 CCTCAGG CC^T[ACGT]AGG
ApaLI 1443 GTGCAC G^TGCAC
AseI 1515 ATTAAT AT^TAAT
AsnI 1515 ATTAAT AT^TAAT
Asp700I 1027 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1275 TGGCCA TGG^CCA
BanII 2195 GGGCTC G[GA]GC[CT]^C
BclI 1860 TGATCA T^GATCA
BlpI 1536 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1536 GCTAAGC GC^T[ACGT]AGC
BsaAI 557 TACGTA [CT]AC^GT[GA]
BsaHI 568 GGCGTC G[GA]^CG[CT]C
BsgI 830 CTGCAC GTGCAG(16/14)
BsmBI 61 GAGACG CGTCTC(1/5)
BsmFI 1547 GTCCC GGGAC(10/14)
BsmI 2210 GCATTC GAATGC(1/-1)
BspHI 1863 TCATGA T^CATGA
BspMI 2046 GCAGGT ACCTGC(4/8)
BsrFI 1098 ACCGGC [GA]^CCGG[CT]
Bst1107I 173 GTATAC GTA^TAC
BstUI 13 CGCG CG^CG
BstXI 2331 CCAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 173 GTATAC GTA^TAC
Bsu36I 475 CCTCAGG CC^T[ACGT]AGG
CelII 1536 GCTAAGC GC^T[ACGT]AGC
CfoI 1155 GCGC GCG^C
Cfr10I 1098 ACCGGC [GA]^CCGG[CT]
DraII 471 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1460 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 475 CCTCAGG CC^T[ACGT]AGG
EcoO109I 471 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 994 GATATC GAT^ATC
FauI 14 GCGGG CCCGC(4/6)
FbaI 1860 TGATCA T^GATCA
HgaI 569 GCGTC GACGC(5/10)
HhaI 1155 GCGC GCG^C
Hin1I 568 GGCGTC G[GA]^CG[CT]C
HinP1I 1155 GCGC G^CGC
HincII 1224 GTCAAC GT[CT]^[GA]AC
HindII 1224 GTCAAC GT[CT]^[GA]AC
KspI 12 CCGCGG CCGC^GG
MluNI 1275 TGGCCA TGG^CCA
MscI 1275 TGGCCA TGG^CCA
MvnI 13 CGCG CG^CG
NcoI 1124 CCATGG C^CATGG
PshAI 2085 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1515 ATTAAT AT^TAAT
PstI 580 CTGCAG CTGCA^G
RcaI 1863 TCATGA T^CATGA
SacII 12 CCGCGG CCGC^GG
SapI 1630 GAAGAGC GCTCTTC(1/4)
ScaI 1110 AGTACT AGT^ACT
SnaBI 557 TACGTA TAC^GTA
XbaI 2907 TCTAGA T^CTAGA
XcmI 1278 CCAGTTATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1027 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 40 GGACC G^G[AT]CC
1641 GGTCC
BbsI 2576 GAAGAC GAAGAC(2/6)
2355 GTCTTC
BpuAI 2576 GAAGAC GAAGAC(2/6)
2355 GTCTTC
BsaBI 2776 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2905 GATCTAGATC
BseRI 406 GAGGAG GAGGAG(10/8)
667 GAGGAG
DsaI 12 CCGCGG C^C[GA][CT]GG
1124 CCATGG
EaeI 1275 TGGCCA [CT]^GGCC[GA]
2771 TGGCCG
HapII 38 CCGG C^CGG
1099 CCGG
HindIII 73 AAGCTT A^AGCTT
442 AAGCTT
HpaII 38 CCGG C^CGG
1099 CCGG
MamI 2776 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2905 GATCTAGATC
MfeI 1103 CAATTG C^AATTG
1542 CAATTG
MslI 1119 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2057 CATTAAAATG
MspI 38 CCGG C^CGG
1099 CCGG
MunI 1103 CAATTG C^AATTG
1542 CAATTG
NlaIV 40 GGACCC GG[ACGT]^[ACGT]CC
984 GGTTCC
PleI 1989 GACTC GAGTC(4/5)
2302 GACTC
SspI 752 AATATT AAT^ATT
1450 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BbvI BfaI
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII