Restriction map for
pFN21AB8343
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AlwI 571 GATCC GGATC(4/5)
AspHI 55 GAGCAC G[AT]GC[AT]^C
BsiHKAI 55 GAGCAC G[AT]GC[AT]^C
BsmFI 198 GTCCC GGGAC(10/14)
BsrI 405 ACTGG ACTGG(1/-1)
BstYI 570 AGATCC [GA]^GATC[CT]
Cac8I 183 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 391 CTCAG C^T[ACGT]AG
DpnII 571 GATC ^GATC
DraII 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeIII 174 GGCC GG^CC
MboI 571 GATC ^GATC
MflI 570 AGATCC [GA]^GATC[CT]
MslI 108 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 498 CATATG CA^TATG
NdeII 571 GATC ^GATC
PflMI 221 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 196 GAGTC GAGTC(4/5)
PshAI 475 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 88 CTGCAG CTGCA^G
Sau3AI 571 GATC ^GATC
SfaNI 642 GATGC GCATC(5/9)
SfcI 88 CTGCAG C^T[GA][CT]AG
SmlI 294 CTCAAG C^T[CT][GA]AG
TfiI 346 GATTC G^A[AT]TC
Van91I 221 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 570 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 47 AGCT AG^CT
317 AGCT
AvaII 209 GGTCC G^G[AT]CC
357 GGTCC
BanI 106 GGCACC G^G[CT][GA]CC
378 GGTGCC
BmyI 55 GAGCAC G[AGT]GC[ACT]^C
379 GTGCCC
BpmI 228 CTGGAG CTGGAG(16/14)
512 CTGGAG
Bsp1286I 55 GAGCAC G[AGT]GC[ACT]^C
379 GTGCCC
DsaI 80 CCATGG C^C[GA][CT]GG
353 CCATGG
HinfI 196 GAGTC G^A[ACGT]TC
346 GATTC
HphI 23 TCACC GGTGA(8/7)
77 TCACC
MaeIII 76 GTCAC ^GT[ACGT]AC
115 GTGAC
NcoI 80 CCATGG C^CATGG
353 CCATGG
NspI 16 ACATGC [GA]CATG^[CT]
127 ACATGC
Tsp45I 76 GTCAC ^GT[GC]AC
115 GTGAC
TspRI 594 CACTG CA[GC]TG[ACGT][ACGT]^
632 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI BbvI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cfr13I
EarI EcoRII EcoT14I Fnu4HI FokI
ItaI Ksp632I MboII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI StyI TseI