Restriction map for
pFN21AB8371
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 958 GTAGAC GT^[AC][GT]AC
AciI 1226 GCGG CCGC(-3/-1)
AcsI 237 GAATTC [GA]^AATT[CT]
AflIII 977 ACATGT A^C[GA][CT]GT
ApaI 1505 GGGCCC GGGCC^C
ApoI 237 GAATTC [GA]^AATT[CT]
AvaI 20 CCCGAG C^[CT]CG[GA]G
BanII 1505 GGGCCC G[GA]GC[CT]^C
BbvI 428 GCAGC GCAGC(8/12)
BclI 531 TGATCA T^GATCA
BmyI 1505 GGGCCC G[AGT]GC[ACT]^C
BseRI 832 GAGGAG GAGGAG(10/8)
BsmBI 967 GAGACG CGTCTC(1/5)
BsoBI 20 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1505 GGGCCC G[AGT]GC[ACT]^C
BspHI 900 TCATGA T^CATGA
BspLU11I 977 ACATGT A^CATGT
BspMI 207 GCAGGT ACCTGC(4/8)
BsrDI 749 GCAATG GCAATG(2/0)
BssSI 412 CTCGTG CACGAG(-5/-1)
BstYI 216 GGATCT [GA]^GATC[CT]
EcoRI 237 GAATTC G^AATTC
EcoT14I 456 CCAAGG C^C[AT][AT]GG
FbaI 531 TGATCA T^GATCA
Fnu4HI 428 GCAGC GC^[ACGT]GC
HgaI 654 GCGTC GACGC(5/10)
HincII 871 GTCAAC GT[CT]^[GA]AC
HindII 871 GTCAAC GT[CT]^[GA]AC
ItaI 428 GCAGC GC^[ACGT]GC
MflI 216 GGATCT [GA]^GATC[CT]
MseI 1174 TTAA T^TAA
NdeI 202 CATATG CA^TATG
NspI 977 ACATGT [GA]CATG^[CT]
PshAI 649 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1134 CTGCAG CTGCA^G
RcaI 900 TCATGA T^CATGA
SexAI 98 ACCTGGT A^CC[AT]GGT
SfaNI 568 GATGC GCATC(5/9)
SpeI 252 ACTAGT A^CTAGT
StuI 1373 AGGCCT AGG^CCT
StyI 456 CCAAGG C^C[AT][AT]GG
Tru9I 1174 TTAA T^TAA
TseI 428 GCAGC G^C[AT]GC
XhoII 216 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BcnI 105 CCGGG CC^[GC]GG
398 CCCGG
Bsp1407I 473 TGTACA T^GTACA
980 TGTACA
BsrGI 473 TGTACA T^GTACA
980 TGTACA
BsrI 987 ACTGG ACTGG(1/-1)
695 CCAGT
BstXI 1070 CCACTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1329 CCAGAGCAGTGG
HapII 105 CCGG C^CGG
399 CCGG
HpaII 105 CCGG C^CGG
399 CCGG
MspI 105 CCGG C^CGG
399 CCGG
NciI 105 CCGGG CC^[GC]GG
398 CCCGG
SfcI 1103 CTACAG C^T[GA][CT]AG
1134 CTGCAG
SmlI 494 CTCAAG C^T[CT][GA]AG
1086 CTCAAG
SspBI 473 TGTACA T^GTACA
980 TGTACA
Tsp45I 244 GTGAC ^GT[GC]AC
1348 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI AvaII BfaI
BpmI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FokI HaeIII
HinfI HphI MaeI MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TfiI Tsp509I
TspRI