Restriction map for
pFN21AB8414
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# NO CUT ENZYMES ( 83 enzymes) #
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AccIII AclI AflII AgeI AhdI
AocI AscI AseI AsnI Asp700I
AspEI AvrII BbeI BfrI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI FseI HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1152 GACGTC GACGT^C
Acc65I 1037 GGTACC G^GTACC
AcyI 1152 GACGTC G[GA]^CG[CT]C
Alw44I 793 GTGCAC G^TGCAC
Aor51HI 2051 AGCGCT AGC^GCT
ApaLI 793 GTGCAC G^TGCAC
Asp718I 1037 GGTACC G^GTACC
AspI 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1529 TGCGCA TGC^GCA
BbsI 1861 GAAGAC GAAGAC(2/6)
BbvCI 1160 CCTCAGC CCTCAGC(-5/-2)
BclI 1626 TGATCA T^GATCA
BpuAI 1861 GAAGAC GAAGAC(2/6)
BsaBI 2492 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1152 GACGTC G[GA]^CG[CT]C
BsmBI 1226 GAGACG CGTCTC(1/5)
BsmI 728 GAATGC GAATGC(1/-1)
BspHI 1661 TCATGA T^CATGA
BstEII 2101 GGTGACC G^GT[ACGT]ACC
BstPI 2101 GGTGACC G^GT[ACGT]ACC
DrdI 736 GACGGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2051 AGCGCT AGC^GCT
EcoO65I 2101 GGTGACC G^GT[ACGT]ACC
EcoRI 2245 GAATTC G^AATTC
EcoRV 2515 GATATC GAT^ATC
EcoT22I 2109 ATGCAT ATGCA^T
FbaI 1626 TGATCA T^GATCA
FspI 1529 TGCGCA TGC^GCA
Hin1I 1152 GACGTC G[GA]^CG[CT]C
HincII 1504 GTCAAC GT[CT]^[GA]AC
HindII 1504 GTCAAC GT[CT]^[GA]AC
HindIII 1469 AAGCTT A^AGCTT
KpnI 1037 GGTACC GGTAC^C
MamI 2492 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2109 ATGCAT ATGCA^T
PaeR7I 255 CTCGAG C^TCGAG
PflFI 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1661 TCATGA T^CATGA
SbfI 2283 CCTGCAGG CCTGCA^GG
SmaI 27 CCCGGG CCC^GGG
SmlI 255 CTCGAG C^T[CT][GA]AG
Sse8387I 2283 CCTGCAGG CCTGCA^GG
StuI 903 AGGCCT AGG^CCT
Tth111I 1699 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 292 CCAAAGGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 255 CTCGAG C^TCGAG
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
366 GTCTAC
AccII 95 CGCG CG^CG
97 CGCG
AcsI 1822 GAATTT [GA]^AATT[CT]
2245 GAATTC
ApaI 197 GGGCCC GGGCC^C
816 GGGCCC
ApoI 1822 GAATTT [GA]^AATT[CT]
2245 GAATTC
BamHI 305 GGATCC G^GATCC
2290 GGATCC
BanI 1037 GGTACC G^G[CT][GA]CC
1789 GGCACC
BanII 197 GGGCCC G[GA]GC[CT]^C
816 GGGCCC
BbrPI 790 CACGTG CAC^GTG
1396 CACGTG
BcgI 633 GCATGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
670 GCAAACAATTCG
BfaI 1595 CTAG C^TAG
2570 CTAG
BglI 1165 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2021 GCCCCCCTGGC
BglII 537 AGATCT A^GATCT
1116 AGATCT
BsaAI 790 CACGTG [CT]AC^GT[GA]
1396 CACGTG
BsaI 1559 GAGACC GGTCTC(1/5)
1598 GGTCTC
BseRI 719 GAGGAG GAGGAG(10/8)
1556 GAGGAG
BspLU11I 2236 ACATGT A^CATGT
2722 ACATGT
BspMI 41 ACCTGC ACCTGC(4/8)
1897 GCAGGT
BstUI 95 CGCG CG^CG
97 CGCG
BstXI 1317 CCATCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1352 CCATGGTGATGG
FauI 1082 GCGGG CCCGC(4/6)
1261 GCGGG
MaeI 1595 CTAG C^TAG
2570 CTAG
MvnI 95 CGCG CG^CG
97 CGCG
PflMI 107 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
395 CCAGCATGTGG
PmaCI 790 CACGTG CAC^GTG
1396 CACGTG
PmlI 790 CACGTG CAC^GTG
1396 CACGTG
PshAI 93 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
349 GACTTCAGTC
TaqI 256 TCGA T^CGA
455 TCGA
TthHB8I 256 TCGA T^CGA
455 TCGA
Van91I 107 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
395 CCAGCATGTGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BbvI BciVI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII