Restriction map for
pFN21AE0466
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FseI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfuI SmaI SmlI SnaBI
SpeI Sse8387I StuI SwaI TaqI
TfiI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 918 GTAC GT^AC
AhdI 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 231 GTGCAC G^TGCAC
ApaLI 231 GTGCAC G^TGCAC
AspEI 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 67 CTCGGG C^[CT]CG[GA]G
AviII 248 TGCGCA TGC^GCA
BanII 377 GGGCTC G[GA]GC[CT]^C
BciVI 750 GGATAC GTATCC(6/5)
BsoBI 67 CTCGGG C^[CT]CG[GA]G
Bsp1407I 917 TGTACA T^GTACA
BspHI 641 TCATGA T^CATGA
BsrBI 631 CCGCTC CCGCTC(-3/-3)
BsrDI 482 GCAATG GCAATG(2/0)
BsrGI 917 TGTACA T^GTACA
BssSI 331 CTCGTG CACGAG(-5/-1)
DrdI 838 GACACTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 299 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 879 GAAGAG CTCTTC(1/4)
EcoNI 53 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 239 CCATGG C^C[AT][AT]GG
FspI 248 TGCGCA TGC^GCA
HinfI 845 GAGTC G^A[ACGT]TC
Ksp632I 879 GAAGAG CTCTTC(1/4)
MslI 234 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 143 GCCGGC GCC^GGC
NcoI 239 CCATGG C^CATGG
NgoMIV 143 GCCGGC G^CCGGC
NspI 802 GCATGC [GA]CATG^[CT]
PleI 845 GAGTC GAGTC(4/5)
PvuII 394 CAGCTG CAG^CTG
RcaI 641 TCATGA T^CATGA
RsaI 918 GTAC GT^AC
SapI 879 GAAGAGC GCTCTTC(1/4)
SfiI 90 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 142 CGCCGGCG C[GA]^CCGG[CT]G
SphI 802 GCATGC GCATG^C
SspBI 917 TGTACA T^GTACA
SspI 715 AATATT AAT^ATT
StyI 239 CCATGG C^C[AT][AT]GG
XcmI 43 CCAGCCCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AcsI 600 GAATTT [GA]^AATT[CT]
702 GAATTT
AcyI 119 GGCGCC G[GA]^CG[CT]C
140 GGCGCC
ApoI 600 GAATTT [GA]^AATT[CT]
702 GAATTT
AspHI 231 GTGCAC G[AT]GC[AT]^C
334 GTGCTC
AvaII 390 GGACC G^G[AT]CC
831 GGACC
BbeI 119 GGCGCC GGCGC^C
140 GGCGCC
BclI 594 TGATCA T^GATCA
638 TGATCA
BcnI 47 CCCGG CC^[GC]GG
189 CCCGG
BfaI 82 CTAG C^TAG
407 CTAG
BpmI 380 CTCCAG CTGGAG(16/14)
842 CTGGAG
BsaHI 119 GGCGCC G[GA]^CG[CT]C
140 GGCGCC
BseRI 15 CTCCTC GAGGAG(10/8)
745 GAGGAG
BsiHKAI 231 GTGCAC G[AT]GC[AT]^C
334 GTGCTC
BsmFI 106 GGGAC GGGAC(10/14)
266 GGGAC
BspMI 260 ACCTGC ACCTGC(4/8)
156 GCAGGT
BstEII 723 GGTGACC G^GT[ACGT]ACC
982 GGTGACC
BstPI 723 GGTGACC G^GT[ACGT]ACC
982 GGTGACC
BstYI 182 GGATCT [GA]^GATC[CT]
941 GGATCT
EaeI 89 TGGCCG [CT]^GGCC[GA]
236 CGGCCA
EcoO65I 723 GGTGACC G^GT[ACGT]ACC
982 GGTGACC
FbaI 594 TGATCA T^GATCA
638 TGATCA
Hin1I 119 GGCGCC G[GA]^CG[CT]C
140 GGCGCC
KasI 119 GGCGCC G^GCGCC
140 GGCGCC
MaeI 82 CTAG C^TAG
407 CTAG
MflI 182 GGATCT [GA]^GATC[CT]
941 GGATCT
MseI 287 TTAA T^TAA
464 TTAA
MspA1I 252 CAGCGG C[AC]G^C[GT]G
394 CAGCTG
NarI 119 GGCGCC GG^CGCC
140 GGCGCC
NciI 47 CCCGG CC^[GC]GG
189 CCCGG
PflMI 835 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
931 CCACTTTTTGG
PstI 151 CTGCAG CTGCA^G
441 CTGCAG
SfaNI 649 GATGC GCATC(5/9)
736 GATGC
SfcI 151 CTGCAG C^T[GA][CT]AG
441 CTGCAG
SfoI 119 GGCGCC GGC^GCC
140 GGCGCC
Tru9I 287 TTAA T^TAA
464 TTAA
Van91I 835 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
931 CCACTTTTTGG
XhoII 182 GGATCT [GA]^GATC[CT]
941 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BanI BbvI BglI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I
Tsp509I TspRI