Restriction map for
pFN21AE3286
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FauI
FseI FspI HpaI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 491 GTCGAC GT^[AC][GT]AC
AciI 13 GCGG CCGC(-3/-1)
AflIII 603 ACATGT A^C[GA][CT]GT
AlwI 1455 GATCC GGATC(4/5)
AseI 1686 ATTAAT AT^TAAT
AsnI 1686 ATTAAT AT^TAAT
Asp700I 1639 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 83 CCCGGG C^[CT]CG[GA]G
BanI 611 GGCGCC G^G[CT][GA]CC
BanII 542 GAGCCC G[GA]GC[CT]^C
BbeI 611 GGCGCC GGCGC^C
BbvCI 811 GCTGAGG CCTCAGC(-5/-2)
BclI 1844 TGATCA T^GATCA
BglII 1093 AGATCT A^GATCT
BsaBI 468 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 83 CCCGGG C^[CT]CG[GA]G
BspLU11I 603 ACATGT A^CATGT
BsrDI 1812 CATTGC GCAATG(2/0)
BstAPI 1318 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1056 GGTGACC G^GT[ACGT]ACC
BstPI 1056 GGTGACC G^GT[ACGT]ACC
BstXI 530 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1093 AGATCT [GA]^GATC[CT]
CfoI 612 GCGC GCG^C
DrdI 353 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1650 CCATGG C^C[GA][CT]GG
EcoO65I 1056 GGTGACC G^GT[ACGT]ACC
EcoRV 1201 GATATC GAT^ATC
FbaI 1844 TGATCA T^GATCA
HaeII 611 GGCGCC [GA]GCGC^[CT]
HgaI 1242 GCGTC GACGC(5/10)
HhaI 612 GCGC GCG^C
HinP1I 612 GCGC G^CGC
HindIII 406 AAGCTT A^AGCTT
KasI 611 GGCGCC G^GCGCC
MamI 468 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1093 AGATCT [GA]^GATC[CT]
MspA1I 1528 CAGCTG C[AC]G^C[GT]G
NarI 611 GGCGCC GG^CGCC
NcoI 1650 CCATGG C^CATGG
PflMI 1650 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1872 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1686 ATTAAT AT^TAAT
PvuII 1528 CAGCTG CAG^CTG
SalI 491 GTCGAC G^TCGAC
SfoI 611 GGCGCC GGC^GCC
SmaI 83 CCCGGG CCC^GGG
SmlI 1976 CTCAAG C^T[CT][GA]AG
SphI 1553 GCATGC GCATG^C
Van91I 1650 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 854 CCACTGGGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1093 AGATCT [GA]^GATC[CT]
XmaCI 83 CCCGGG C^CCGGG
XmaI 83 CCCGGG C^CCGGG
XmnI 1639 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 611 GGCGCC G[GA]^CG[CT]C
1241 GGCGTC
BmyI 369 GTGCCC G[AGT]GC[ACT]^C
542 GAGCCC
BsaHI 611 GGCGCC G[GA]^CG[CT]C
1241 GGCGTC
BsmI 1628 GAATGC GAATGC(1/-1)
1153 GCATTC
Bsp1286I 369 GTGCCC G[AGT]GC[ACT]^C
542 GAGCCC
Bsp1407I 705 TGTACA T^GTACA
793 TGTACA
BsrGI 705 TGTACA T^GTACA
793 TGTACA
EarI 1643 CTCTTC CTCTTC(1/4)
1474 GAAGAG
EcoNI 33 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
174 CCTGGCCCAGG
EcoT22I 1084 ATGCAT ATGCA^T
1733 ATGCAT
Hin1I 611 GGCGCC G[GA]^CG[CT]C
1241 GGCGTC
HincII 491 GTCGAC GT[CT]^[GA]AC
1747 GTCAAC
HindII 491 GTCGAC GT[CT]^[GA]AC
1747 GTCAAC
Ksp632I 1643 CTCTTC CTCTTC(1/4)
1474 GAAGAG
NsiI 1084 ATGCAT ATGCA^T
1733 ATGCAT
ScaI 1353 AGTACT AGT^ACT
1882 AGTACT
SspBI 705 TGTACA T^GTACA
793 TGTACA
TaqI 492 TCGA T^CGA
1989 TCGA
TthHB8I 492 TCGA T^CGA
1989 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AcsI AfaI AluI AlwNI AocI
ApoI AvaII BbvI BcnI BfaI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI