Restriction map for
pFN21AE9502
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AlwNI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HinfI HpaI KpnI KspI MamI
MfeI MluI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SbfI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 306 CGCG CG^CG
AccIII 968 TCCGGA T^CCGGA
Alw44I 675 GTGCAC G^TGCAC
AlwI 161 GGATC GGATC(4/5)
AocI 210 CCTAAGG CC^T[ACGT]AGG
ApaLI 675 GTGCAC G^TGCAC
AvaI 610 CCCGAG C^[CT]CG[GA]G
AviII 707 TGCGCA TGC^GCA
BbeI 216 GGCGCC GGCGC^C
BbsI 228 GAAGAC GAAGAC(2/6)
BbvCI 909 CCTCAGC CCTCAGC(-5/-2)
BlpI 594 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 594 GCTGAGC GC^T[ACGT]AGC
BpuAI 228 GAAGAC GAAGAC(2/6)
BsaWI 968 TCCGGA [AT]^CCGG[AT]
BseAI 968 TCCGGA T^CCGGA
BseRI 808 CTCCTC GAGGAG(10/8)
BsiEI 191 CGGCCG CG[GA][CT]^CG
BsmAI 73 GTCTC GTCTC(1/5)
BsmBI 72 CGTCTC CGTCTC(1/5)
BsoBI 610 CCCGAG C^[CT]CG[GA]G
BspEI 968 TCCGGA T^CCGGA
BsrBI 732 CCGCTC CCGCTC(-3/-3)
BssSI 924 CTCGTG CACGAG(-5/-1)
BstAPI 312 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 306 CGCG CG^CG
BstYI 161 GGATCT [GA]^GATC[CT]
Bsu36I 210 CCTAAGG CC^T[ACGT]AGG
CelII 594 GCTGAGC GC^T[ACGT]AGC
DpnII 162 GATC ^GATC
EagI 191 CGGCCG C^GGCCG
EarI 277 CTCTTC CTCTTC(1/4)
EclXI 191 CGGCCG C^GGCCG
Eco52I 191 CGGCCG C^GGCCG
Eco81I 210 CCTAAGG CC^T[ACGT]AGG
EcoT14I 743 CCAAGG C^C[AT][AT]GG
FspI 707 TGCGCA TGC^GCA
HaeII 216 GGCGCC [GA]GCGC^[CT]
KasI 216 GGCGCC G^GCGCC
Ksp632I 277 CTCTTC CTCTTC(1/4)
MboI 162 GATC ^GATC
MflI 161 GGATCT [GA]^GATC[CT]
MroI 968 TCCGGA T^CCGGA
MspA1I 8 CCGCTG C[AC]G^C[GT]G
MvnI 306 CGCG CG^CG
NarI 216 GGCGCC GG^CGCC
NdeII 162 GATC ^GATC
NlaIII 462 CATG CATG^
NspI 461 ACATGC [GA]CATG^[CT]
PflMI 583 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 367 GTCGAC G^TCGAC
SapI 276 GCTCTTC GCTCTTC(1/4)
Sau3AI 162 GATC ^GATC
ScaI 323 AGTACT AGT^ACT
SexAI 428 ACCTGGT A^CC[AT]GGT
SfaNI 392 GCATC GCATC(5/9)
SfoI 216 GGCGCC GGC^GCC
StyI 743 CCAAGG C^C[AT][AT]GG
TaqI 368 TCGA T^CGA
TthHB8I 368 TCGA T^CGA
Van91I 583 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 954 TCTAGA T^CTAGA
XhoII 161 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 242 ACGTGT A^C[GA][CT]GT
644 ACACGT
Asp700I 933 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
972 GAAGAACTTC
BalI 93 TGGCCA TGG^CCA
440 TGGCCA
BcnI 158 CCGGG CC^[GC]GG
480 CCGGG
BpmI 387 CTCCAG CTGGAG(16/14)
737 CTGGAG
BsmFI 453 GTCCC GGGAC(10/14)
580 GTCCC
BstEII 234 GGTGACC G^GT[ACGT]ACC
771 GGTGACC
BstF5I 198 CATCC GGATG(2/0)
249 CATCC
BstPI 234 GGTGACC G^GT[ACGT]ACC
771 GGTGACC
DraII 451 AGGTCCC [GA]G^G[ACGT]CC[CT]
578 AGGTCCC
DsaI 604 CCGTGG C^C[GA][CT]GG
854 CCACGG
EcoO109I 451 AGGTCCC [GA]G^G[ACGT]CC[CT]
578 AGGTCCC
EcoO65I 234 GGTGACC G^GT[ACGT]ACC
771 GGTGACC
FokI 198 CATCC GGATG(9/13)
249 CATCC
HincII 298 GTCAAC GT[CT]^[GA]AC
367 GTCGAC
HindII 298 GTCAAC GT[CT]^[GA]AC
367 GTCGAC
MluNI 93 TGGCCA TGG^CCA
440 TGGCCA
MscI 93 TGGCCA TGG^CCA
440 TGGCCA
MslI 681 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
882 CATCAGTATG
NciI 158 CCGGG CC^[GC]GG
480 CCGGG
PpuMI 451 AGGTCCC [GA]G^G[AT]CC[CT]
578 AGGTCCC
PstI 319 CTGCAG CTGCA^G
574 CTGCAG
XmnI 933 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
972 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbvI
BfaI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeI
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NlaIV RsaI Sau96I
ScrFI SfcI TseI Tsp45I TspRI