Restriction map for
pFN21AE9405
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI KasI KpnI KspI
MaeII MfeI MflI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 21 enzymes) #
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AcsI 427 AAATTC [GA]^AATT[CT]
AlwNI 465 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 427 AAATTC [GA]^AATT[CT]
BbvCI 827 CCTCAGC CCTCAGC(-5/-2)
BciVI 418 GTATCC GTATCC(6/5)
BfaI 286 CTAG C^TAG
BpmI 137 CTGGAG CTGGAG(16/14)
BsaBI 201 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 70 GAGACC GGTCTC(1/5)
BseRI 1056 GAGGAG GAGGAG(10/8)
BsgI 548 GTGCAG GTGCAG(16/14)
BspMI 637 ACCTGC ACCTGC(4/8)
BstF5I 924 CATCC GGATG(2/0)
EcoNI 1008 CCTCACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 866 CCTTGG C^C[AT][AT]GG
FokI 924 CATCC GGATG(9/13)
MaeI 286 CTAG C^TAG
MamI 201 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PstI 354 CTGCAG CTGCA^G
SfcI 354 CTGCAG C^T[GA][CT]AG
StyI 866 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 324 GAGCTC G[AT]GC[AT]^C
517 GAGCTC
BbsI 194 GAAGAC GAAGAC(2/6)
100 GTCTTC
BcnI 184 CCCGG CC^[GC]GG
930 CCGGG
BpuAI 194 GAAGAC GAAGAC(2/6)
100 GTCTTC
BsiHKAI 324 GAGCTC G[AT]GC[AT]^C
517 GAGCTC
BsmFI 585 GTCCC GGGAC(10/14)
616 GTCCC
BsrDI 254 CATTGC GCAATG(2/0)
651 CATTGC
Cac8I 463 GCCAGC GC[ACGT]^[ACGT]GC
485 GCCTGC
EarI 503 CTCTTC CTCTTC(1/4)
1062 GAAGAG
HapII 185 CCGG C^CGG
930 CCGG
HincII 385 GTTGAC GT[CT]^[GA]AC
889 GTCAAC
HindII 385 GTTGAC GT[CT]^[GA]AC
889 GTCAAC
HpaII 185 CCGG C^CGG
930 CCGG
Ksp632I 503 CTCTTC CTCTTC(1/4)
1062 GAAGAG
MseI 572 TTAA T^TAA
598 TTAA
MspA1I 122 CAGCTG C[AC]G^C[GT]G
882 CAGCTG
MspI 185 CCGG C^CGG
930 CCGG
NciI 184 CCCGG CC^[GC]GG
930 CCGGG
NdeI 449 CATATG CA^TATG
579 CATATG
PflMI 401 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
464 CCAGCATCTGG
PvuII 122 CAGCTG CAG^CTG
882 CAGCTG
SacI 324 GAGCTC GAGCT^C
517 GAGCTC
SfaNI 280 GCATC GCATC(5/9)
467 GCATC
TaqI 203 TCGA T^CGA
914 TCGA
Tru9I 572 TTAA T^TAA
598 TTAA
TthHB8I 203 TCGA T^CGA
914 TCGA
Van91I 401 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
464 CCAGCATCTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
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AciI AluI AvaII BalI BanII
BbvI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI HaeIII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MslI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I Tsp509I TspRI