Restriction map for
pFN21AB5667
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BclI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NdeII NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 93 CGCG CG^CG
AcyI 205 GGCGCC G[GA]^CG[CT]C
AflII 736 CTTAAG C^TTAAG
AlwNI 19 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 66 AGCGCT AGC^GCT
BbeI 205 GGCGCC GGCGC^C
BbvCI 472 GCTGAGG CCTCAGC(-5/-2)
BfrI 736 CTTAAG C^TTAAG
BsaHI 205 GGCGCC G[GA]^CG[CT]C
BsmFI 336 GTCCC GGGAC(10/14)
BsmI 996 GCATTC GAATGC(1/-1)
BsrBI 895 GAGCGG CCGCTC(-3/-3)
BsrFI 211 GCCGGC [GA]^CCGG[CT]
BstUI 93 CGCG CG^CG
Cfr10I 211 GCCGGC [GA]^CCGG[CT]
EaeI 285 TGGCCG [CT]^GGCC[GA]
EarI 564 GAAGAG CTCTTC(1/4)
Eco47III 66 AGCGCT AGC^GCT
EcoT14I 830 CCTTGG C^C[AT][AT]GG
EcoT22I 994 ATGCAT ATGCA^T
Hin1I 205 GGCGCC G[GA]^CG[CT]C
KasI 205 GGCGCC G^GCGCC
Ksp632I 564 GAAGAG CTCTTC(1/4)
KspI 92 CCGCGG CCGC^GG
MslI 721 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 93 CGCG CG^CG
NaeI 211 GCCGGC GCC^GGC
NarI 205 GGCGCC GG^CGCC
NgoMIV 211 GCCGGC G^CCGGC
NsiI 994 ATGCAT ATGCA^T
PflMI 819 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 25 CTGCAG CTGCA^G
SacII 92 CCGCGG CCGC^GG
SfaNI 586 GCATC GCATC(5/9)
SfcI 25 CTGCAG C^T[GA][CT]AG
SfiI 237 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 205 GGCGCC GGC^GCC
SmlI 736 CTTAAG C^T[CT][GA]AG
StyI 830 CCTTGG C^C[AT][AT]GG
TaqI 560 TCGA T^CGA
TthHB8I 560 TCGA T^CGA
Van91I 819 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 874 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 316 GAATTC [GA]^AATT[CT]
510 GAATTC
ApoI 316 GAATTC [GA]^AATT[CT]
510 GAATTC
BfaI 367 CTAG C^TAG
875 CTAG
BglI 238 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCCGACTGGC
BseRI 255 GAGGAG GAGGAG(10/8)
483 GAGGAG
BsmAI 4 GAGAC GTCTC(1/5)
571 GTCTC
EcoRI 316 GAATTC G^AATTC
510 GAATTC
HaeII 66 AGCGCT [GA]GCGC^[CT]
205 GGCGCC
MaeI 367 CTAG C^TAG
875 CTAG
MaeIII 82 GTGAC ^GT[ACGT]AC
418 GTCAC
NlaIII 651 CATG CATG^
744 CATG
Tsp45I 82 GTGAC ^GT[GC]AC
418 GTCAC
Tsp509I 317 AATT ^AATT
511 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI ApaI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI TfiI Tru9I TseI
TspRI