Restriction map for
pFN21AB8973
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BseRI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FseI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 162 GACGCC G[GA]^CG[CT]C
AhdI 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 484 GTGCAC G^TGCAC
ApaI 1038 GGGCCC GGGCC^C
ApaLI 484 GTGCAC G^TGCAC
AspEI 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 785 TGCGCA TGC^GCA
BamHI 25 GGATCC G^GATCC
BsaAI 856 TACGTG [CT]AC^GT[GA]
BsaHI 162 GACGCC G[GA]^CG[CT]C
BsaI 257 GAGACC GGTCTC(1/5)
BsiEI 465 CGACCG CG[GA][CT]^CG
BspLU11I 425 ACATGT A^CATGT
BsrBI 846 CCGCTC CCGCTC(-3/-3)
BsrDI 147 GCAATG GCAATG(2/0)
BsrFI 403 ACCGGC [GA]^CCGG[CT]
BstAPI 744 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1054 TTCGAA TT^CGAA
BstEII 708 GGTCACC G^GT[ACGT]ACC
BstPI 708 GGTCACC G^GT[ACGT]ACC
BstYI 25 GGATCC [GA]^GATC[CT]
Cfr10I 403 ACCGGC [GA]^CCGG[CT]
DraIII 613 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 412 CTCTTC CTCTTC(1/4)
EcoNI 738 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 708 GGTCACC G^GT[ACGT]ACC
EcoT22I 295 ATGCAT ATGCA^T
FspI 785 TGCGCA TGC^GCA
HgaI 162 GACGC GACGC(5/10)
Hin1I 162 GACGCC G[GA]^CG[CT]C
HincII 841 GTCAAC GT[CT]^[GA]AC
HindII 841 GTCAAC GT[CT]^[GA]AC
HinfI 70 GACTC G^A[ACGT]TC
Ksp632I 412 CTCTTC CTCTTC(1/4)
MaeIII 709 GTCAC ^GT[ACGT]AC
MflI 25 GGATCC [GA]^GATC[CT]
NsiI 295 ATGCAT ATGCA^T
NspV 1054 TTCGAA TT^CGAA
PleI 70 GACTC GAGTC(4/5)
PpuMI 271 GGGACCC [GA]G^G[AT]CC[CT]
PstI 628 CTGCAG CTGCA^G
PvuII 631 CAGCTG CAG^CTG
SacI 1074 GAGCTC GAGCT^C
SfcI 628 CTGCAG C^T[GA][CT]AG
SfuI 1054 TTCGAA TT^CGAA
SphI 391 GCATGC GCATG^C
StuI 647 AGGCCT AGG^CCT
Tsp45I 709 GTCAC ^GT[GC]AC
XhoII 25 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 349 GTCTAC GT^[AC][GT]AC
853 GTCTAC
AfaI 429 GTAC GT^AC
636 GTAC
AflIII 284 ACGTGT A^C[GA][CT]GT
425 ACATGT
AlwI 26 GATCC GGATC(4/5)
25 GGATC
AlwNI 62 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
745 CAGGGCCTG
AspHI 484 GTGCAC G[AT]GC[AT]^C
1074 GAGCTC
AvaII 272 GGACC G^G[AT]CC
330 GGTCC
BclI 116 TGATCA T^GATCA
725 TGATCA
BpmI 72 CTCCAG CTGGAG(16/14)
169 CTGGAG
BsgI 862 CTGCAC GTGCAG(16/14)
601 GTGCAG
BsiHKAI 484 GTGCAC G[AT]GC[AT]^C
1074 GAGCTC
BsmBI 397 CGTCTC CGTCTC(1/5)
160 GAGACG
BsrI 168 ACTGG ACTGG(1/-1)
371 ACTGG
FbaI 116 TGATCA T^GATCA
725 TGATCA
HaeII 460 AGCGCC [GA]GCGC^[CT]
764 AGCGCC
MspA1I 577 CCGCTG C[AC]G^C[GT]G
631 CAGCTG
NcoI 569 CCATGG C^CATGG
876 CCATGG
PflMI 612 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
657 CCATGCCCTGG
RsaI 429 GTAC GT^AC
636 GTAC
TaqI 959 TCGA T^CGA
1055 TCGA
TthHB8I 959 TCGA T^CGA
1055 TCGA
Van91I 612 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
657 CCATGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI BalI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TseI
TspRI XcmI