Restriction map for
pFN21AE1396
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tru9I
Tsp45I Tsp509I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 170 CGCG CG^CG
AciI 110 GCGG CCGC(-3/-1)
AflIII 169 ACGCGT A^C[GA][CT]GT
AluI 130 AGCT AG^CT
BamHI 5 GGATCC G^GATCC
BanI 45 GGTGCC G^G[CT][GA]CC
BcgI 178 CGAAAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 126 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 126 GCTCAGC GC^T[ACGT]AGC
BsiEI 111 CGGCCG CG[GA][CT]^CG
BsrFI 113 GCCGGT [GA]^CCGG[CT]
BssKI 55 CCAGG ^CC[ACGT]GG
BstNI 55 CCAGG CC^[AT]GG
BstUI 170 CGCG CG^CG
BstYI 5 GGATCC [GA]^GATC[CT]
CelII 126 GCTCAGC GC^T[ACGT]AGC
Cfr10I 113 GCCGGT [GA]^CCGG[CT]
DdeI 127 CTCAG C^T[ACGT]AG
EagI 111 CGGCCG C^GGCCG
EarI 31 CTCTTC CTCTTC(1/4)
EclXI 111 CGGCCG C^GGCCG
Eco52I 111 CGGCCG C^GGCCG
EcoRII 55 CCAGG ^CC[AT]GG
HapII 114 CCGG C^CGG
HgaI 168 GACGC GACGC(5/10)
HpaII 114 CCGG C^CGG
Ksp632I 31 CTCTTC CTCTTC(1/4)
MflI 5 GGATCC [GA]^GATC[CT]
MluI 169 ACGCGT A^CGCGT
MspA1I 129 CAGCTG C[AC]G^C[GT]G
MspI 114 CCGG C^CGG
MvaI 55 CCAGG CC^[AT]GG
MvnI 170 CGCG CG^CG
MwoI 176 GCCGAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 61 CATG CATG^
PflMI 55 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 101 GAGTC GAGTC(4/5)
PvuII 129 CAGCTG CAG^CTG
SapI 30 GCTCTTC GCTCTTC(1/4)
ScrFI 55 CCAGG CC^[ACGT]GG
SspI 89 AATATT AAT^ATT
TaqI 166 TCGA T^CGA
TfiI 68 GAATC G^A[AT]TC
TthHB8I 166 TCGA T^CGA
Van91I 55 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 5 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 6 GATCC GGATC(4/5)
5 GGATC
AspHI 124 GTGCTC G[AT]GC[AT]^C
162 GTGCTC
BbvI 131 GCTGC GCAGC(8/12)
185 GCTGC
BmyI 124 GTGCTC G[AGT]GC[ACT]^C
162 GTGCTC
BsiHKAI 124 GTGCTC G[AT]GC[AT]^C
162 GTGCTC
Bsp1286I 124 GTGCTC G[AGT]GC[ACT]^C
162 GTGCTC
DpnII 6 GATC ^GATC
153 GATC
EaeI 111 CGGCCG [CT]^GGCC[GA]
174 TGGCCG
EcoNI 49 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
135 CCTTCCAAAGG
HaeIII 112 GGCC GG^CC
175 GGCC
HinfI 68 GAATC G^A[ACGT]TC
101 GAGTC
MboI 6 GATC ^GATC
153 GATC
NdeII 6 GATC ^GATC
153 GATC
NlaIV 5 GGATCC GG[ACGT]^[ACGT]CC
45 GGTGCC
Sau3AI 6 GATC ^GATC
153 GATC
TseI 131 GCTGC G^C[AT]GC
185 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
BsiYI BslI Fnu4HI ItaI MboII
MnlI