Restriction map for
pFN21AE0270
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BbrPI BbsI BcgI BclI
BfaI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FspI HgaI HindIII HpaI KpnI
Ksp632I MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcyI 10 GGCGCC G[GA]^CG[CT]C
AflIII 717 ACATGT A^C[GA][CT]GT
AhdI 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 677 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 179 AGCGCT AGC^GCT
AspEI 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3 GGATCC G^GATCC
BanI 10 GGCGCC G^G[CT][GA]CC
BanII 57 GGGCTC G[GA]GC[CT]^C
BbeI 10 GGCGCC GGCGC^C
BbvCI 323 CCTCAGC CCTCAGC(-5/-2)
BciVI 659 GTATCC GTATCC(6/5)
BglI 102 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 281 AGATCT A^GATCT
BmyI 57 GGGCTC G[AGT]GC[ACT]^C
BsaHI 10 GGCGCC G[GA]^CG[CT]C
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsmAI 382 GAGAC GTCTC(1/5)
BsmFI 287 GGGAC GGGAC(10/14)
Bsp1286I 57 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 717 ACATGT A^CATGT
BsrFI 111 GCCGGC [GA]^CCGG[CT]
BsrI 532 ACTGG ACTGG(1/-1)
BstXI 167 CCAGCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 111 GCCGGC [GA]^CCGG[CT]
EagI 113 CGGCCG C^GGCCG
Eam1105I 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 113 CGGCCG C^GGCCG
Eco47III 179 AGCGCT AGC^GCT
Eco52I 113 CGGCCG C^GGCCG
EcoRV 367 GATATC GAT^ATC
FseI 110 GGCCGGCC GGCCGG^CC
Hin1I 10 GGCGCC G[GA]^CG[CT]C
HincII 229 GTTGAC GT[CT]^[GA]AC
HindII 229 GTTGAC GT[CT]^[GA]AC
HinfI 308 GATTC G^A[ACGT]TC
KasI 10 GGCGCC G^GCGCC
KspI 150 CCGCGG CCGC^GG
MaeIII 669 GTTAC ^GT[ACGT]AC
MseI 637 TTAA T^TAA
MslI 531 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 150 CCGCGG C[AC]G^C[GT]G
NaeI 111 GCCGGC GCC^GGC
NarI 10 GGCGCC GG^CGCC
NgoMIV 111 GCCGGC G^CCGGC
NlaIII 718 CATG CATG^
NspI 717 ACATGT [GA]CATG^[CT]
PstI 19 CTGCAG CTGCA^G
SacII 150 CCGCGG CCGC^GG
SfoI 10 GGCGCC GGC^GCC
StuI 206 AGGCCT AGG^CCT
TaqI 602 TCGA T^CGA
TfiI 308 GATTC G^A[AT]TC
Tru9I 637 TTAA T^TAA
Tsp509I 270 AATT ^AATT
TspRI 531 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 602 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsiYI 105 CCCCAGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
150 CCGCGGCGTGG
BslI 105 CCCCAGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
150 CCGCGGCGTGG
BspMI 129 GCAGGT ACCTGC(4/8)
295 GCAGGT
BstF5I 407 GGATG GGATG(2/0)
536 GGATG
BstYI 3 GGATCC [GA]^GATC[CT]
281 AGATCT
Cfr13I 29 GGGCC G^G[ACGT]CC
84 GGGCC
DsaI 150 CCGCGG C^C[GA][CT]GG
221 CCGTGG
EaeI 78 CGGCCA [CT]^GGCC[GA]
113 CGGCCG
EcoT14I 344 CCAAGG C^C[AT][AT]GG
560 CCAAGG
FokI 407 GGATG GGATG(9/13)
536 GGATG
HphI 185 TCACC GGTGA(8/7)
494 TCACC
MboII 411 GAAGA GAAGA(8/7)
436 GAAGA
MflI 3 GGATCC [GA]^GATC[CT]
281 AGATCT
Sau96I 29 GGGCC G^G[ACGT]CC
84 GGGCC
SfcI 19 CTGCAG C^T[GA][CT]AG
248 CTGTAG
StyI 344 CCAAGG C^C[AT][AT]GG
560 CCAAGG
XhoII 3 GGATCC [GA]^GATC[CT]
281 AGATCT
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BsaJI BssKI BstNI BstUI
Cac8I CfoI DdeI DpnII EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV Sau3AI ScrFI
SfaNI TseI