##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp45I      Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AvaII          45  GGACC                G^G[AT]CC
BanI            4  GGTGCC               G^G[CT][GA]CC
BcgI          383  CGAAGAAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          102  CCCGG                CC^[GC]GG
BmyI            5  GTGCCC               G[AGT]GC[ACT]^C
BsaWI         244  TCCGGT               [AT]^CCGG[AT]
BsgI           96  CTGCAC               GTGCAG(16/14)
BsmI          237  GCATTC               GAATGC(1/-1)
Bsp1286I        5  GTGCCC               G[AGT]GC[ACT]^C
BsrBI         106  GAGCGG               CCGCTC(-3/-3)
BstNI          26  CCTGG                CC^[AT]GG
DdeI          143  CTGAG                C^T[ACGT]AG
DsaI          419  CCATGG               C^C[GA][CT]GG
EaeI          113  TGGCCG               [CT]^GGCC[GA]
EcoRII         26  CCTGG                ^CC[AT]GG
HphI          295  GGTGA                GGTGA(8/7)
MaeII          13  ACGT                 A^CGT
MseI          286  TTAA                 T^TAA
MspA1I        409  CAGCTG               C[AC]G^C[GT]G
MvaI           26  CCTGG                CC^[AT]GG
MwoI           89  GCTGCGCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          102  CCCGG                CC^[GC]GG
NcoI          419  CCATGG               C^CATGG
NlaIII        420  CATG                 CATG^
PvuII         409  CAGCTG               CAG^CTG
SfaNI          53  GCATC                GCATC(5/9)
SmlI           40  CTCAAG               C^T[CT][GA]AG
TaqI          404  TCGA                 T^CGA
Tru9I         286  TTAA                 T^TAA
TthHB8I       404  TCGA                 T^CGA
XcmI          401  CCATCGAGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI           51  CCGC                 CCGC(-3/-1)
              108  GCGG                
AcsI          298  GAATTT               [GA]^AATT[CT]
              367  GAATTT              
AluI          188  AGCT                 AG^CT
              410  AGCT                
ApoI          298  GAATTT               [GA]^AATT[CT]
              367  GAATTT              
BssKI          26  CCTGG                ^CC[ACGT]GG
              102  CCCGG               
BstF5I        129  CATCC                GGATG(2/0)
              110  GGATG               
EcoT14I       291  CCTTGG               C^C[AT][AT]GG
              419  CCATGG              
FokI          129  CATCC                GGATG(9/13)
              110  GGATG               
HapII         103  CCGG                 C^CGG
              245  CCGG                
HpaII         103  CCGG                 C^CGG
              245  CCGG                
MspI          103  CCGG                 C^CGG
              245  CCGG                
NlaIV           4  GGTGCC               GG[ACGT]^[ACGT]CC
              201  GGAGCC              
ScrFI          26  CCTGG                CC^[ACGT]GG
              102  CCCGG               
StyI          291  CCTTGG               C^C[AT][AT]GG
              419  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

BbvI        BsaJI       BsiYI       BslI        BsrI        
Cac8I       CfoI        Cfr13I      Fnu4HI      HaeIII      
HhaI        HinP1I      ItaI        MboII       MnlI        
Sau96I      TseI        Tsp509I     TspRI