Restriction map for
pFN21AB4483
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbrPI BcgI BclI BfrI BglI
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstXI ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HindIII KspI MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1432 GACGTC GACGT^C
Acc65I 1508 GGTACC G^GTACC
AccI 523 GTATAC GT^[AC][GT]AC
AcsI 1384 GAATTC [GA]^AATT[CT]
AocI 468 CCTCAGG CC^T[ACGT]AGG
Aor51HI 826 AGCGCT AGC^GCT
ApaI 17 GGGCCC GGGCC^C
ApoI 1384 GAATTC [GA]^AATT[CT]
Asp718I 1508 GGTACC G^GTACC
AvrII 549 CCTAGG C^CTAGG
BalI 1544 TGGCCA TGG^CCA
BbsI 1078 GTCTTC GAAGAC(2/6)
BciVI 893 GTATCC GTATCC(6/5)
BlnI 549 CCTAGG C^CTAGG
BpuAI 1078 GTCTTC GAAGAC(2/6)
BsaI 794 GGTCTC GGTCTC(1/5)
BsaWI 713 TCCGGT [AT]^CCGG[AT]
BseRI 381 GAGGAG GAGGAG(10/8)
BssSI 1261 CACGAG CACGAG(-5/-1)
Bst1107I 523 GTATAC GTA^TAC
BstZ17I 523 GTATAC GTA^TAC
Bsu36I 468 CCTCAGG CC^T[ACGT]AGG
DrdI 1069 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1561 CTCTTC CTCTTC(1/4)
Eco47III 826 AGCGCT AGC^GCT
Eco81I 468 CCTCAGG CC^T[ACGT]AGG
EcoNI 303 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1384 GAATTC G^AATTC
EcoT22I 1448 ATGCAT ATGCA^T
HincII 1306 GTTAAC GT[CT]^[GA]AC
HindII 1306 GTTAAC GT[CT]^[GA]AC
HinfI 247 GAGTC G^A[ACGT]TC
HpaI 1306 GTTAAC GTT^AAC
KpnI 1508 GGTACC GGTAC^C
Ksp632I 1561 CTCTTC CTCTTC(1/4)
MluNI 1544 TGGCCA TGG^CCA
MscI 1544 TGGCCA TGG^CCA
MseI 1307 TTAA T^TAA
MslI 477 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1448 ATGCAT ATGCA^T
PleI 247 GAGTC GAGTC(4/5)
SacI 1264 GAGCTC GAGCT^C
SapI 1560 GCTCTTC GCTCTTC(1/4)
SmaI 80 CCCGGG CCC^GGG
SmlI 1034 CTCAAG C^T[CT][GA]AG
Tru9I 1307 TTAA T^TAA
Tsp509I 1385 AATT ^AATT
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 506 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1192 CAGCTGCTG
AspHI 75 GAGCAC G[AT]GC[AT]^C
1264 GAGCTC
AspI 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1072 GACTTCGTC
BbeI 120 GGCGCC GGCGC^C
1213 GGCGCC
BbvCI 1362 CCTCAGC CCTCAGC(-5/-2)
330 GCTGAGG
BfaI 550 CTAG C^TAG
832 CTAG
BlpI 492 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
Bpu1102I 492 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
BsaAI 970 TACGTG [CT]AC^GT[GA]
1051 TACGTG
BsiHKAI 75 GAGCAC G[AT]GC[AT]^C
1264 GAGCTC
BsrBI 430 CCGCTC CCGCTC(-3/-3)
1123 GAGCGG
BsrFI 1553 ACCGGC [GA]^CCGG[CT]
1574 ACCGGC
BstEII 915 GGTTACC G^GT[ACGT]ACC
1290 GGTCACC
BstPI 915 GGTTACC G^GT[ACGT]ACC
1290 GGTCACC
CelII 492 GCTGAGC GC^T[ACGT]AGC
1197 GCTGAGC
Cfr10I 1553 ACCGGC [GA]^CCGG[CT]
1574 ACCGGC
DraII 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
260 GGGGCCT
DsaI 115 CCGTGG C^C[GA][CT]GG
920 CCGTGG
EaeI 1025 TGGCCG [CT]^GGCC[GA]
1544 TGGCCA
EcoO109I 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
260 GGGGCCT
EcoO65I 915 GGTTACC G^GT[ACGT]ACC
1290 GGTCACC
KasI 120 GGCGCC G^GCGCC
1213 GGCGCC
MaeI 550 CTAG C^TAG
832 CTAG
NarI 120 GGCGCC GG^CGCC
1213 GGCGCC
PflFI 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1072 GACTTCGTC
SexAI 527 ACCAGGT A^CC[AT]GGT
1139 ACCTGGT
SfoI 120 GGCGCC GGC^GCC
1213 GGCGCC
TaqI 379 TCGA T^CGA
1352 TCGA
Tth111I 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1072 GACTTCGTC
TthHB8I 379 TCGA T^CGA
1352 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbvI BcnI BglII BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI XhoII