Restriction map for
pFN21AE1115
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstF5I BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI TspRI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 157 GGCGTC G[GA]^CG[CT]C
BalI 323 TGGCCA TGG^CCA
BbvI 407 GCAGC GCAGC(8/12)
BpmI 565 CTCCAG CTGGAG(16/14)
BsaHI 157 GGCGTC G[GA]^CG[CT]C
BsaI 184 GAGACC GGTCTC(1/5)
BsgI 299 GTGCAG GTGCAG(16/14)
BsiEI 84 CGATCG CG[GA][CT]^CG
Bsp1407I 482 TGTACA T^GTACA
BsrBI 132 CCGCTC CCGCTC(-3/-3)
BsrDI 31 GCAATG GCAATG(2/0)
BsrGI 482 TGTACA T^GTACA
BsrI 378 CCAGT ACTGG(1/-1)
BstEII 261 GGTCACC G^GT[ACGT]ACC
BstPI 261 GGTCACC G^GT[ACGT]ACC
Cac8I 361 GCCCGC GC[ACGT]^[ACGT]GC
CfoI 129 GCGC GCG^C
DsaI 113 CCGTGG C^C[GA][CT]GG
EcoNI 411 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 261 GGTCACC G^GT[ACGT]ACC
EcoT14I 508 CCTTGG C^C[AT][AT]GG
FauI 362 CCCGC CCCGC(4/6)
HaeII 128 AGCGCC [GA]GCGC^[CT]
HgaI 158 GCGTC GACGC(5/10)
HhaI 129 GCGC GCG^C
Hin1I 157 GGCGTC G[GA]^CG[CT]C
HinP1I 129 GCGC G^CGC
MaeII 338 ACGT A^CGT
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
MslI 190 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 498 GCCATTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshAI 153 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 84 CGATCG CGAT^CG
SspBI 482 TGTACA T^GTACA
SspI 461 AATATT AAT^ATT
StyI 508 CCTTGG C^C[AT][AT]GG
TaqI 534 TCGA T^CGA
TseI 407 GCAGC G^C[AT]GC
Tsp45I 262 GTCAC ^GT[GC]AC
Tsp509I 59 AATT ^AATT
TthHB8I 534 TCGA T^CGA
XcmI 499 CCATTCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
232 GACATACTGTC
AspEI 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
232 GACATACTGTC
BanII 282 GAGCCC G[GA]GC[CT]^C
400 GGGCTC
BbsI 150 GAAGAC GAAGAC(2/6)
160 GTCTTC
BciVI 36 GTATCC GTATCC(6/5)
381 GTATCC
BmyI 282 GAGCCC G[AGT]GC[ACT]^C
400 GGGCTC
BpuAI 150 GAAGAC GAAGAC(2/6)
160 GTCTTC
BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG
508 CCTTGG
Bsp1286I 282 GAGCCC G[AGT]GC[ACT]^C
400 GGGCTC
DraI 61 TTTAAA TTT^AAA
457 TTTAAA
EaeI 311 TGGCCG [CT]^GGCC[GA]
323 TGGCCA
Eam1105I 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
232 GACATACTGTC
Fnu4HI 131 GCCGC GC^[ACGT]GC
407 GCAGC
HaeIII 312 GGCC GG^CC
324 GGCC
ItaI 131 GCCGC GC^[ACGT]GC
407 GCAGC
MaeIII 262 GTCAC ^GT[ACGT]AC
389 GTTAC
NlaIV 281 GGAGCC GG[ACGT]^[ACGT]CC
401 GGCTCC
PleI 414 GAGTC GAGTC(4/5)
561 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AfaI BsiYI BslI BsmAI
BssKI BstNI DdeI DpnII EcoRII
HinfI HphI MboI MboII MnlI
MseI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SexAI TfiI Tru9I