Restriction map for
pFN21AB4181
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BcgI BfrI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstAPI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HindIII KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 1378 GACGTC GACGT^C
AcsI 2491 GAATTT [GA]^AATT[CT]
ApoI 2491 GAATTT [GA]^AATT[CT]
AvrII 331 CCTAGG C^CTAGG
BbeI 1303 GGCGCC GGCGC^C
BbrPI 1564 CACGTG CAC^GTG
BciVI 1357 GTATCC GTATCC(6/5)
BlnI 331 CCTAGG C^CTAGG
BsaWI 427 ACCGGA [AT]^CCGG[AT]
BsmBI 773 GAGACG CGTCTC(1/5)
BsmI 1850 GCATTC GAATGC(1/-1)
BspLU11I 2390 ACATGT A^CATGT
BsrBI 2606 CCGCTC CCGCTC(-3/-3)
BsrDI 2121 CATTGC GCAATG(2/0)
BstBI 2698 TTCGAA TT^CGAA
HaeII 1303 GGCGCC [GA]GCGC^[CT]
HpaI 1867 GTTAAC GTT^AAC
KasI 1303 GGCGCC G^GCGCC
KspI 672 CCGCGG CCGC^GG
NarI 1303 GGCGCC GG^CGCC
NcoI 272 CCATGG C^CATGG
NspV 2698 TTCGAA TT^CGAA
PmaCI 1564 CACGTG CAC^GTG
PmlI 1564 CACGTG CAC^GTG
PpuMI 2333 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 235 GAGCTC GAGCT^C
SacII 672 CCGCGG CCGC^GG
SalI 1381 GTCGAC G^TCGAC
SapI 1269 GCTCTTC GCTCTTC(1/4)
SfoI 1303 GGCGCC GGC^GCC
SfuI 2698 TTCGAA TT^CGAA
SmlI 940 CTTGAG C^T[CT][GA]AG
SphI 1256 GCATGC GCATG^C
SspI 871 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 1381 GTCGAC GT^[AC][GT]AC
1540 GTCTAC
AcyI 1303 GGCGCC G[GA]^CG[CT]C
1378 GACGTC
AflIII 2390 ACATGT A^C[GA][CT]GT
2582 ACACGT
AocI 54 CCTGAGG CC^T[ACGT]AGG
580 CCTGAGG
AvaI 766 CCCGAG C^[CT]CG[GA]G
2474 CTCGGG
BalI 33 TGGCCA TGG^CCA
1508 TGGCCA
BamHI 668 GGATCC G^GATCC
998 GGATCC
BbvCI 574 CCTCAGC CCTCAGC(-5/-2)
550 GCTGAGG
BclI 1784 TGATCA T^GATCA
2429 TGATCA
BfaI 332 CTAG C^TAG
2650 CTAG
BglII 1479 AGATCT A^GATCT
2646 AGATCT
BsaHI 1303 GGCGCC G[GA]^CG[CT]C
1378 GACGTC
BsaI 431 GAGACC GGTCTC(1/5)
1318 GAGACC
BsoBI 766 CCCGAG C^[CT]CG[GA]G
2474 CTCGGG
BspMI 434 ACCTGC ACCTGC(4/8)
2675 ACCTGC
BstEII 813 GGTCACC G^GT[ACGT]ACC
1800 GGTGACC
BstPI 813 GGTCACC G^GT[ACGT]ACC
1800 GGTGACC
Bsu36I 54 CCTGAGG CC^T[ACGT]AGG
580 CCTGAGG
DraIII 70 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2260 CACCTTGTG
Eco81I 54 CCTGAGG CC^T[ACGT]AGG
580 CCTGAGG
EcoO65I 813 GGTCACC G^GT[ACGT]ACC
1800 GGTGACC
FbaI 1784 TGATCA T^GATCA
2429 TGATCA
HgaI 775 GACGC GACGC(5/10)
1057 GCGTC
Hin1I 1303 GGCGCC G[GA]^CG[CT]C
1378 GACGTC
MaeI 332 CTAG C^TAG
2650 CTAG
MluNI 33 TGGCCA TGG^CCA
1508 TGGCCA
MscI 33 TGGCCA TGG^CCA
1508 TGGCCA
PstI 255 CTGCAG CTGCA^G
442 CTGCAG
ScaI 164 AGTACT AGT^ACT
2279 AGTACT
SfcI 255 CTGCAG C^T[GA][CT]AG
442 CTGCAG
StuI 707 AGGCCT AGG^CCT
756 AGGCCT
XcmI 1266 CCAGCTCTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1514 CCATTGAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII