Restriction map for
pFN21AE0296
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AseI
AsnI Asp700I AspHI AspI AviII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BsaBI
BseAI BseRI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DraIII DrdI EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI Ksp632I MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 640 GGTACC G^GTACC
AfaI 641 GTAC GT^AC
AscI 142 GGCGCGCC GG^CGCGCC
Asp718I 640 GGTACC G^GTACC
AvrII 801 CCTAGG C^CTAGG
BlnI 801 CCTAGG C^CTAGG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsaWI 841 ACCGGA [AT]^CCGG[AT]
BsiEI 624 CGGCCG CG[GA][CT]^CG
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrI 464 ACTGG ACTGG(1/-1)
BssSI 583 CTCGTG CACGAG(-5/-1)
CelII 45 GCTGAGC GC^T[ACGT]AGC
EagI 624 CGGCCG C^GGCCG
EclXI 624 CGGCCG C^GGCCG
Eco52I 624 CGGCCG C^GGCCG
HincII 925 GTTGAC GT[CT]^[GA]AC
HindII 925 GTTGAC GT[CT]^[GA]AC
HinfI 845 GAGTC G^A[ACGT]TC
KpnI 640 GGTACC GGTAC^C
MaeIII 884 GTGAC ^GT[ACGT]AC
NaeI 340 GCCGGC GCC^GGC
NgoMIV 340 GCCGGC G^CCGGC
PflMI 330 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 845 GAGTC GAGTC(4/5)
PpuMI 453 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 641 GTAC GT^AC
SfaNI 746 GCATC GCATC(5/9)
SmlI 616 CTTGAG C^T[CT][GA]AG
StuI 359 AGGCCT AGG^CCT
Tsp45I 884 GTGAC ^GT[GC]AC
TspRI 440 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 330 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 416 CCAGCGTGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
886 GACTGCGTGTC
AlwNI 64 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
331 CAGCGCCTG
ApaI 271 GGGCCC GGGCC^C
430 GGGCCC
AspEI 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
886 GACTGCGTGTC
BbsI 822 GAAGAC GAAGAC(2/6)
292 GTCTTC
BfaI 802 CTAG C^TAG
856 CTAG
BpuAI 822 GAAGAC GAAGAC(2/6)
292 GTCTTC
BsaI 127 GAGACC GGTCTC(1/5)
776 GAGACC
BsgI 309 CTGCAC GTGCAG(16/14)
469 CTGCAC
BspMI 323 ACCTGC ACCTGC(4/8)
560 ACCTGC
BssHII 143 GCGCGC G^CGCGC
495 GCGCGC
DdeI 46 CTGAG C^T[ACGT]AG
896 CTAAG
Eam1105I 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
886 GACTGCGTGTC
HgaI 28 GACGC GACGC(5/10)
410 GCGTC
MaeI 802 CTAG C^TAG
856 CTAG
MslI 591 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
762 CACCGTCATG
PstI 442 CTGCAG CTGCA^G
562 CTGCAG
SfcI 442 CTGCAG C^T[GA][CT]AG
562 CTGCAG
TaqI 98 TCGA T^CGA
722 TCGA
TthHB8I 98 TCGA T^CGA
722 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV SacII Sau96I
ScrFI SfoI StyI TseI