Restriction map for
pFN21AB8259
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 360 GTCGAC GT^[AC][GT]AC
AfaI 565 GTAC GT^AC
AvaII 456 GGACC G^G[AT]CC
AviII 413 TGCGCA TGC^GCA
BamHI 321 GGATCC G^GATCC
BbrPI 466 CACGTG CAC^GTG
BglI 307 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 130 CTCCAG CTGGAG(16/14)
BsaAI 466 CACGTG [CT]AC^GT[GA]
BsaWI 387 TCCGGT [AT]^CCGG[AT]
BsmAI 210 GTCTC GTCTC(1/5)
BsmFI 527 GGGAC GGGAC(10/14)
BsmI 514 GAATGC GAATGC(1/-1)
BsrBI 265 GAGCGG CCGCTC(-3/-3)
BsrDI 195 CATTGC GCAATG(2/0)
BstAPI 416 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 54 GGTGACC G^GT[ACGT]ACC
BstPI 54 GGTGACC G^GT[ACGT]ACC
DraII 314 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 80 CCATGG C^C[GA][CT]GG
EcoO109I 314 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 54 GGTGACC G^GT[ACGT]ACC
EcoT22I 516 ATGCAT ATGCA^T
FspI 413 TGCGCA TGC^GCA
HaeII 491 AGCGCC [GA]GCGC^[CT]
NcoI 80 CCATGG C^CATGG
NsiI 516 ATGCAT ATGCA^T
NspI 278 GCATGC [GA]CATG^[CT]
PaeR7I 297 CTCGAG C^TCGAG
PmaCI 466 CACGTG CAC^GTG
PmlI 466 CACGTG CAC^GTG
PstI 448 CTGCAG CTGCA^G
RsaI 565 GTAC GT^AC
SacI 177 GAGCTC GAGCT^C
SalI 360 GTCGAC G^TCGAC
SapI 63 GAAGAGC GCTCTTC(1/4)
SfcI 448 CTGCAG C^T[GA][CT]AG
SmlI 297 CTCGAG C^T[CT][GA]AG
SphI 278 GCATGC GCATG^C
XhoI 297 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 177 GAGCTC G[AT]GC[AT]^C
733 GAGCAC
AvaI 140 CCCGAG C^[CT]CG[GA]G
297 CTCGAG
BanI 354 GGCACC G^G[CT][GA]CC
390 GGTGCC
BanII 177 GAGCTC G[GA]GC[CT]^C
841 GAGCCC
BsiHKAI 177 GAGCTC G[AT]GC[AT]^C
733 GAGCAC
BsoBI 140 CCCGAG C^[CT]CG[GA]G
297 CTCGAG
BstYI 321 GGATCC [GA]^GATC[CT]
698 AGATCC
EarI 13 GAAGAG CTCTTC(1/4)
63 GAAGAG
EcoT14I 80 CCATGG C^C[AT][AT]GG
716 CCAAGG
HincII 157 GTCAAC GT[CT]^[GA]AC
360 GTCGAC
HindII 157 GTCAAC GT[CT]^[GA]AC
360 GTCGAC
Ksp632I 13 GAAGAG CTCTTC(1/4)
63 GAAGAG
MaeIII 55 GTGAC ^GT[ACGT]AC
397 GTCAC
MflI 321 GGATCC [GA]^GATC[CT]
698 AGATCC
MslI 540 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
813 CACACAGGTG
MspA1I 440 CCGCTG C[AC]G^C[GT]G
882 CCGCTG
StyI 80 CCATGG C^C[AT][AT]GG
716 CCAAGG
Tsp45I 55 GTGAC ^GT[GC]AC
397 GTCAC
XhoII 321 GGATCC [GA]^GATC[CT]
698 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI BalI
BbvI BcnI BmyI BsaJI BseRI
BsgI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MluNI MnlI MscI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I