Restriction map for
pFN21AB6661
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BanI BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BpmI BsaAI BsgI BsiWI
BsmI BspDI BspHI BspLU11I BsrDI
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoRI EcoT22I
FseI FspI HindIII HpaI KasI
KpnI Ksp632I MluI MslI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AatII 243 GACGTC GACGT^C
AccIII 494 TCCGGA T^CCGGA
AcyI 243 GACGTC G[GA]^CG[CT]C
ApaI 225 GGGCCC GGGCC^C
AspHI 513 GAGCAC G[AT]GC[AT]^C
AspI 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 75 CCTAGG C^CTAGG
BalI 551 TGGCCA TGG^CCA
BamHI 425 GGATCC G^GATCC
BbsI 737 GAAGAC GAAGAC(2/6)
BbvCI 1069 GCTGAGG CCTCAGC(-5/-2)
BclI 782 TGATCA T^GATCA
BfaI 76 CTAG C^TAG
BlnI 75 CCTAGG C^CTAGG
BlpI 123 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
BpuAI 737 GAAGAC GAAGAC(2/6)
BsaHI 243 GACGTC G[GA]^CG[CT]C
BsaI 1265 GAGACC GGTCTC(1/5)
BseAI 494 TCCGGA T^CCGGA
BseRI 765 GAGGAG GAGGAG(10/8)
BsiEI 210 CGGCCG CG[GA][CT]^CG
BsiHKAI 513 GAGCAC G[AT]GC[AT]^C
BsmBI 363 CGTCTC CGTCTC(1/5)
Bsp1407I 437 TGTACA T^GTACA
BspEI 494 TCCGGA T^CCGGA
BspMI 656 ACCTGC ACCTGC(4/8)
BsrBI 613 GAGCGG CCGCTC(-3/-3)
BsrGI 437 TGTACA T^GTACA
BsrI 1041 CCAGT ACTGG(1/-1)
BssHII 273 GCGCGC G^CGCGC
BstEII 1240 GGTGACC G^GT[ACGT]ACC
BstPI 1240 GGTGACC G^GT[ACGT]ACC
CelII 123 GCTGAGC GC^T[ACGT]AGC
CpoI 260 CGGTCCG CG^G[AT]CCG
DraIII 327 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 210 CGGCCG C^GGCCG
EclXI 210 CGGCCG C^GGCCG
Eco52I 210 CGGCCG C^GGCCG
EcoO65I 1240 GGTGACC G^GT[ACGT]ACC
EcoRV 883 GATATC GAT^ATC
FbaI 782 TGATCA T^GATCA
HaeII 183 GGCGCT [GA]GCGC^[CT]
Hin1I 243 GACGTC G[GA]^CG[CT]C
HincII 355 GTTGAC GT[CT]^[GA]AC
HindII 355 GTTGAC GT[CT]^[GA]AC
MaeI 76 CTAG C^TAG
MaeII 244 ACGT A^CGT
MboII 737 GAAGA GAAGA(8/7)
MfeI 1196 CAATTG C^AATTG
MluNI 551 TGGCCA TGG^CCA
MroI 494 TCCGGA T^CCGGA
MscI 551 TGGCCA TGG^CCA
MunI 1196 CAATTG C^AATTG
NspI 1166 GCATGC [GA]CATG^[CT]
PflFI 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 260 CGGTCCG CG^G[AT]CCG
SmaI 229 CCCGGG CCC^GGG
SphI 1166 GCATGC GCATG^C
SspBI 437 TGTACA T^GTACA
TaqI 835 TCGA T^CGA
Tth111I 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 835 TCGA T^CGA
XmaCI 229 CCCGGG C^CCGGG
XmaI 229 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 438 GTAC GT^AC
654 GTAC
AvaI 34 CCCGAG C^[CT]CG[GA]G
229 CCCGGG
BsaWI 320 TCCGGT [AT]^CCGG[AT]
494 TCCGGA
BsoBI 34 CCCGAG C^[CT]CG[GA]G
229 CCCGGG
BssSI 118 CTCGTG CACGAG(-5/-1)
676 CTCGTG
EaeI 210 CGGCCG [CT]^GGCC[GA]
551 TGGCCA
KspI 136 CCGCGG CCGC^GG
221 CCGCGG
MseI 731 TTAA T^TAA
817 TTAA
NcoI 59 CCATGG C^CATGG
1260 CCATGG
PflMI 326 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1255 CCAACCCATGG
PvuII 199 CAGCTG CAG^CTG
430 CAGCTG
RsaI 438 GTAC GT^AC
654 GTAC
SacII 136 CCGCGG CCGC^GG
221 CCGCGG
SmlI 868 CTTGAG C^T[CT][GA]AG
1130 CTCAAG
Tru9I 731 TTAA T^TAA
817 TTAA
Van91I 326 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1255 CCAACCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AvaII BanII BbvI
BcnI BmyI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MamI MboI MflI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI XhoII