Restriction map for
pFN21ASDF0400
(Circular) (Six-base) MAPSORT of: pFN21ASDF0400.seq Check: 4945 from: 1 to: 6907
pFN21ASDF0400 6907 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4717 278
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4250 1937
Size: 2313 4594
Acc65I G'GTAC_C
Cuts at: 4228 4228
Size: 6907
AclI AA'CG_TT
Cuts at: 1563 3258 5034 5407 1563
Size: 1695 1776 373 3063
Fragments arranged by size:
3063 1776 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 3587 3802 4970 6354 6622 1632
Size: 1550 405 215 1168 1384 268 1917
Fragments arranged by size:
1917 1550 1384 1168 405 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6710
AflIII A'CryG_T
Cuts at: 1203 5806 6783 1203
Size: 4603 977 1327
Fragments arranged by size:
4603 1327 977
AgeI A'CCGG_T
Cuts at: 3384 5738 3384
Size: 2354 4553
AhdI GACnn_n'nnGTC
Cuts at: 5636 5636
Size: 6907
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 5287
AlwNI CAG_nnn'CTG
Cuts at: 2272 3037 4650 6222 2272
Size: 765 1613 1572 2957
Fragments arranged by size:
2957 1613 1572 765
ApaI G_GGCC'C
Cuts at: 2059 2893 4004 4100 2059
Size: 834 1111 96 4866
Fragments arranged by size:
4866 1111 834 96
ApaLI G'TGCA_C
Cuts at: 4964 6120 4964
Size: 1156 5751
ApoI r'AATT_y
Cuts at: 1493 2337 4216 4442 4594 6703 1493
Size: 844 1879 226 152 2109 1697
Fragments arranged by size:
2109 1879 1697 844 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5461 160
Size: 872 4429 1606
Fragments arranged by size:
4429 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 2886 3985 4072 4232 1870
Size: 73 334 609 1099 87 160 4545
Fragments arranged by size:
4545 1099 609 334 160 87 73
BamHI G'GATC_C
Cuts at: 4237 4604 4237
Size: 367 6540
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2247 2740 3942 4151
Size: 325 217 627 278 182 493 1202 209
Cuts at: 4151 4228 5683 618
Size: 77 1455 1842
Fragments arranged by size:
1842 1455 1202 627 493 325 278 217
209 182 77
BanII G_rGCy'C
Cuts at: 1391 2059 2187 2292 2893 2946 3771 3809 4004
Size: 668 128 105 601 53 825 38 195
Cuts at: 4004 4100 4226 1391
Size: 96 126 4072
Fragments arranged by size:
4072 825 668 601 195 128 126 105
96 53 38
BbeI G_GCGC'C
Cuts at: 2251 2744 2251
Size: 493 6414
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 2752 3517 928
Size: 971 821 32 765 4318
Fragments arranged by size:
4318 971 821 765 32
BbvCI CC'TCA_GC
Cuts at: 2642 2642
Size: 6907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5098 5132 1903
Size: 34 3161 34 3678
Fragments arranged by size:
3678 3161 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4799 6009 4799
Size: 1210 5697
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6907
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2328 2897 3370
Size: 107 122 71 631 605 656 569 473
Cuts at: 3370 5518 136
Size: 2148 1525
Fragments arranged by size:
2148 1525 656 631 605 569 473 122
107 71
BglII A'GATC_T
Cuts at: 6902 6902
Size: 6907
BlpI GC'TnA_GC
Cuts at: 2387 3842 4324 2387
Size: 1455 482 4970
Fragments arranged by size:
4970 1455 482
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2483 2893 3666 3793 4004 4100 4156
Size: 269 424 410 773 127 211 96 56
Cuts at: 4156 4968 6124 1790
Size: 812 1156 2573
Fragments arranged by size:
2573 1156 812 773 424 410 269 211
127 96 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6907
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4638 5596 442
Size: 416 53 83 399 24 3221 958 1753
Fragments arranged by size:
3221 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 4011 2626
Size: 1385 5522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 2867 3014 3125 3559 3589 5567 1239
Size: 1220 408 147 111 434 30 1978 2579
Fragments arranged by size:
2579 1978 1220 434 408 147 111 30
Bpu10I CC'TnA_GC
Cuts at: 2642 2642
Size: 6907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5032 5897 6195 5032
Size: 865 298 5744
Fragments arranged by size:
5744 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5570 882
Size: 617 4071 2219
Fragments arranged by size:
4071 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4204 4698 493
Size: 711 3000 494 2702
Fragments arranged by size:
3000 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6901 6901
Size: 6907
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 2248 2741 4714
Size: 53 83 186 936 590 125 493 1973
Cuts at: 4714 5096 5731 275
Size: 382 635 1451
Fragments arranged by size:
1973 1451 936 635 590 493 382 186
125 83 53
BsaWI w'CCGG_w
Cuts at: 3384 3906 5340 5738 6012 6159 6570 3384
Size: 522 1434 398 274 147 411 3721
Fragments arranged by size:
3721 1434 522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 3002 3032 3287
Size: 30 165 30 666 30 903 30 255
Cuts at: 3287 3317 3791 3821 3944 3974 4001 4031 1178
Size: 30 474 30 123 30 27 30 4054
Fragments arranged by size:
4054 903 666 474 255 165 123 30
30 30 30 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2630 2654 2755 2864 3023 3311 3320
Size: 907 537 24 101 109 159 288 9
Cuts at: 3320 3437 3626 3685 3821 1186
Size: 117 189 59 136 4272
Fragments arranged by size:
4272 907 537 288 189 159 136 117
109 101 59 24 9
BseYI C'CCAG_C
Cuts at: 2722 3276 4184 6110 6606 2722
Size: 554 908 1926 496 3023
Fragments arranged by size:
3023 1926 908 554 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 2810 1163
Size: 573 480 594 5260
Fragments arranged by size:
5260 594 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4094 4357 5118 6146 1540
Size: 275 186 2093 263 761 1028 2301
Fragments arranged by size:
2301 2093 1028 761 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 3636 4968 5053 6124 3636
Size: 1332 85 1071 4419
Fragments arranged by size:
4419 1332 1071 85
BsmI GAATG_Cn'
Cuts at: 4418 4511 4418
Size: 93 6814
BsmBI CGTCTCn'nnnn_
Cuts at: 3196 3871 3196
Size: 675 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2187 2292 2483 2893 2946 3636
Size: 399 269 128 105 191 410 53 690
Cuts at: 3636 3666 3771 3793 3809 4004 4100 4156 4226
Size: 30 105 22 16 195 96 56 70
Cuts at: 4226 4968 5053 6124 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 690 410 399 269 195
191 128 105 105 96 85 70 56
53 30 22 16
BspEI T'CCGG_A
Cuts at: 3906 6570 3906
Size: 2664 4243
BspHI T'CATG_A
Cuts at: 4796 6526 4796
Size: 1730 5177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2928 3045 3916 4262 844
Size: 1547 537 117 871 346 3489
Fragments arranged by size:
3489 1547 871 537 346 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 2104 4794 1394
Size: 429 230 51 2690 3507
Fragments arranged by size:
3507 2690 429 230 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 5402 5576 65
Size: 1183 4154 174 1396
Fragments arranged by size:
4154 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 3384 3396 5551 5738 1700
Size: 110 416 1158 12 2155 187 2869
Fragments arranged by size:
2869 2155 1158 416 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 2616 3303 96
Size: 2520 687 3700
Fragments arranged by size:
3700 2520 687
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6907
BssSI C'ACGA_G
Cuts at: 4967 5979 4967
Size: 1012 5895
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2203 794
Size: 1409 5498
BstBI TT'CG_AA
Cuts at: 2007 5711 2007
Size: 3704 3203
BstEII G'GTnAC_C
Cuts at: 1184 3355 3472 1184
Size: 2171 117 4619
Fragments arranged by size:
4619 2171 117
BstXI CCAn_nnnn'nTGG
Cuts at: 2483 2483
Size: 6907
BstYI r'GATC_y
Cuts at: 1972 4237 4604 4989 5006 6458 6544 6556 6902
Size: 2265 367 385 17 1452 86 12 346
Cuts at: 6902 1972
Size: 1977
Fragments arranged by size:
2265 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2033 2187 3288 3336 4088 4116 513
Size: 552 968 154 1101 48 752 28 3304
Fragments arranged by size:
3304 1101 968 752 552 154 48 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3037 487
Size: 683 56 1811 4357
Fragments arranged by size:
4357 1811 683 56
BtsI GCAGTG_nn'
Cuts at: 3666 4423 5217 5237 3666
Size: 757 794 20 5336
Fragments arranged by size:
5336 794 757 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6872
DraI TTT'AAA
Cuts at: 4211 4211
Size: 6907
DraIII CAC_nnn'GTG
Cuts at: 3949 3949
Size: 6907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5914 809
Size: 507 4598 1802
Fragments arranged by size:
4598 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3168 3781 4091
Size: 54 1425 50 275 18 1338 613 310
Cuts at: 4091 4354 5243 6872 8
Size: 263 889 1629 43
Fragments arranged by size:
1629 1425 1338 889 613 310 275 263
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4091 4354 1537
Size: 275 2279 263 4090
Fragments arranged by size:
4090 2279 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2579 3494 3536 3719 4837 1353
Size: 977 249 915 42 183 1118 3423
Fragments arranged by size:
3423 1118 977 915 249 183 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2426 2615 2704 4153 5494 5868
Size: 1064 138 837 189 89 1449 1341 374
Cuts at: 5868 6014 387
Size: 146 1280
Fragments arranged by size:
1449 1341 1280 1064 837 374 189 146
138 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 2867 3014 3125 3182 3559 3587
Size: 393 827 408 147 111 57 377 28
Cuts at: 3587 3589 3802 4970 5567 6354 6622 1239
Size: 2 213 1168 597 787 268 1524
Fragments arranged by size:
1524 1168 827 787 597 408 393 377
268 213 147 111 57 28 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2448 3950 2255
Size: 193 1502 5212
Fragments arranged by size:
5212 1502 193
EcoO109I rG'GnC_Cy
Cuts at: 2056 2281 2320 2453 2553 3074 3955 4001 4084
Size: 225 39 133 100 521 881 46 83
Cuts at: 4084 4097 2056
Size: 13 4866
Fragments arranged by size:
4866 881 521 225 133 100 83 46
39 13
EcoRI G'AATT_C
Cuts at: 2337 4216 4594 2337
Size: 1879 378 4650
Fragments arranged by size:
4650 1879 378
FspI TGC'GCA
Cuts at: 5413 5413
Size: 6907
HaeII r_GCGC'y
Cuts at: 1678 2164 2227 2251 2306 2744 2754 2970 6054
Size: 486 63 24 55 438 10 216 3084
Cuts at: 6054 1678
Size: 2531
Fragments arranged by size:
3084 2531 486 438 216 63 55 24
10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3374 3406 3944 3976 5548
Size: 32 135 32 1802 32 538 32 1572
Cuts at: 5548 5580 5622 5654 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1802 1572 538 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2929 3064 3133 4251 4497 669
Size: 988 281 991 135 69 1118 246 3079
Fragments arranged by size:
3079 1118 991 988 281 246 135 69
HindIII A'AGCT_T
Cuts at: 748 2407 4023 748
Size: 1659 1616 3632
Fragments arranged by size:
3632 1659 1616
HpaI GTT'AAC
Cuts at: 4497 4497
Size: 6907
KasI G'GCGC_C
Cuts at: 2247 2740 2247
Size: 493 6414
KpnI G_GTAC'C
Cuts at: 4232 4232
Size: 6907
MfeI C'AATT_G
Cuts at: 4506 4506
Size: 6907
MluI A'CGCG_T
Cuts at: 6783 6783
Size: 6907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6021 6205 1305
Size: 4716 184 2007
Fragments arranged by size:
4716 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3170 3783 10
Size: 54 1425 343 1338 613 3134
Fragments arranged by size:
3134 1425 1338 613 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2786 3479 4865 5224 5383 518
Size: 648 1620 693 1386 359 159 2042
Fragments arranged by size:
2042 1620 1386 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2189 2215 2269 2650 2869 3103 3237
Size: 570 13 26 54 381 219 234 134
Cuts at: 3237 3916 4090 4118 4188 4323 5000 6148 6393
Size: 679 174 28 70 135 677 1148 245
Cuts at: 6393 1606
Size: 2120
Fragments arranged by size:
2120 1148 679 677 570 381 245 234
219 174 135 134 70 54 28 26
13
NaeI GCC'GGC
Cuts at: 2228 2228
Size: 6907
NarI GG'CG_CC
Cuts at: 2248 2741 2248
Size: 493 6414
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6355
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6907
NgoMIV G'CCGG_C
Cuts at: 2226 2226
Size: 6907
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6907
NotI GC'GGCC_GC
Cuts at: 4354 4354
Size: 6907
NruI TCG'CGA
Cuts at: 3964 3964
Size: 6907
NspI r_CATG'y
Cuts at: 1132 1229 2540 4265 5810 1132
Size: 97 1311 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 1311 97
PasI CC'CwG_GG
Cuts at: 1348 2060 3044 3196 3667 1348
Size: 712 984 152 471 4588
Fragments arranged by size:
4588 984 712 471 152
PciI A'CATG_T
Cuts at: 5806 5806
Size: 6907
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2976 3802 4064 1108
Size: 1868 826 262 3951
Fragments arranged by size:
3951 1868 826 262
PfoI T'CCnGG_A
Cuts at: 3747 3747
Size: 6907
PmeI GTTT'AAAC
Cuts at: 4211 4211
Size: 6907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4976 5008 6489 6521 4976
Size: 32 1481 32 5362
Fragments arranged by size:
5362 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3074 3955 3074
Size: 881 6026
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6907
PsiI TTA'TAA
Cuts at: 4477 4477
Size: 6907
PspOMI G'GGCC_C
Cuts at: 2055 2889 4000 4096 2055
Size: 834 1111 96 4866
Fragments arranged by size:
4866 1111 834 96
PstI C_TGCA'G
Cuts at: 830 3661 4259 830
Size: 2831 598 3478
Fragments arranged by size:
3478 2831 598
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6907
PvuII CAG'CTG
Cuts at: 2176 2869 3103 3237 3916 2176
Size: 693 234 134 679 5167
Fragments arranged by size:
5167 693 679 234 134
SacII CC_GC'GG
Cuts at: 2190 4091 4119 2190
Size: 1901 28 4978
Fragments arranged by size:
4978 1901 28
SalI G'TCGA_C
Cuts at: 1936 4249 1936
Size: 2313 4594
SanDI GG'GwC_CC
Cuts at: 3074 3074
Size: 6907
SbfI CC_TGCA'GG
Cuts at: 4259 4259
Size: 6907
ScaI AGT'ACT
Cuts at: 2990 5155 6867 2990
Size: 2165 1712 3030
Fragments arranged by size:
3030 2165 1712
SexAI A'CCwGG_T
Cuts at: 3945 3945
Size: 6907
SfcI C'TryA_G
Cuts at: 826 1045 3657 4255 5390 6071 6262 826
Size: 219 2612 598 1135 681 191 1471
Fragments arranged by size:
2612 1471 1135 681 598 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2897 2897
Size: 6907
SfoI GGC'GCC
Cuts at: 2249 2742 2249
Size: 493 6414
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
SgrAI Cr'CCGG_yG
Cuts at: 1700 2226 1700
Size: 526 6381
SmaI CCC'GGG
Cuts at: 1872 2279 2888 4074 4234 1872
Size: 407 609 1186 160 4545
Fragments arranged by size:
4545 1186 609 407 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5011 5912 6174 820
Size: 197 926 3068 901 262 1553
Fragments arranged by size:
3068 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4204 4698 493
Size: 3711 494 2702
Fragments arranged by size:
3711 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6907
SphI G_CATG'C
Cuts at: 1132 4265 1132
Size: 3133 3774
SspI AAT'ATT
Cuts at: 5 52 4831 5
Size: 47 4779 2081
Fragments arranged by size:
4779 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6907
StyI C'CwwG_G
Cuts at: 513 1065 2284 2754 3763 4346 513
Size: 552 1219 470 1009 583 3074
Fragments arranged by size:
3074 1219 1009 583 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2331 4934 4951 5104 5289 5734 325
Size: 1255 751 2603 17 153 185 445 1498
Fragments arranged by size:
2603 1498 1255 751 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 2988 3303
Size: 274 80 33 51 1443 639 372 315
Cuts at: 3303 3555 5153 6865 96
Size: 252 1598 1712 138
Fragments arranged by size:
1712 1598 1443 639 372 315 274 252
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 6867 6899 2069
Size: 32 4766 32 2077
Fragments arranged by size:
4766 2077 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6907
XbaI T'CTAG_A
Cuts at: 4243 4243
Size: 6907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 3445 2501
Size: 944 5963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6907
XmaI C'CCGG_G
Cuts at: 1870 2277 2886 4072 4232 1870
Size: 407 609 1186 160 4545
Fragments arranged by size:
4545 1186 609 407 160
XmnI GAAnn'nnTTC
Cuts at: 5036 5036
Size: 6907
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4715 276
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NsiI
PacI PmlI PspXI PsrI RsrII SacI SapI SrfI
SwaI