Restriction map for
pFN21ASDF0390
(Circular) (Six-base) MAPSORT of: pFN21ASDF0390.seq Check: 4663 from: 1 to: 5554
pFN21ASDF0390 5554 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 2059 2326 2356 2428 2838 3297 4869 2059
Size: 267 30 72 410 459 1572 2744
Fragments arranged by size:
2744 1572 459 410 267 72 30
ApaI G_GGCC'C
Cuts at: 2155 2155
Size: 5554
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2762 2863 3089 3241 5350 1493
Size: 1269 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2341 2438 2559 2879 1870
Size: 73 398 97 121 320 4545
Fragments arranged by size:
4545 398 320 121 97 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2875 4330 618
Size: 325 217 627 385 703 1455 1842
Fragments arranged by size:
1842 1455 703 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 2155 2271 2873 1391
Size: 764 116 602 4072
Fragments arranged by size:
4072 764 602 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2229 2564 4165
Size: 107 122 71 631 605 557 335 1601
Cuts at: 4165 136
Size: 1525
Fragments arranged by size:
1601 1525 631 605 557 335 122 107
71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2320 2659 3615 4771 1790
Size: 365 165 339 956 1156 2573
Fragments arranged by size:
2573 1156 956 365 339 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2537 4214 1239
Size: 911 387 1677 2579
Fragments arranged by size:
2579 1677 911 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2851 3345 493
Size: 711 1647 494 2702
Fragments arranged by size:
2702 1647 711 494
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 2613 3361 3743
Size: 53 83 186 936 753 327 748 382
Cuts at: 3743 4378 275
Size: 635 1451
Fragments arranged by size:
1451 936 753 748 635 382 327 186
83 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2148 2178 2711 2741 1178
Size: 30 165 30 745 30 533 30 3991
Fragments arranged by size:
3991 745 533 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2534 2719 2825 1186
Size: 1348 185 106 3915
Fragments arranged by size:
3915 1348 185 106
BseYI C'CCAG_C
Cuts at: 2091 2126 2148 4757 5253 2091
Size: 35 22 2609 496 2392
Fragments arranged by size:
2609 2392 496 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2497 1163
Size: 573 761 4220
Fragments arranged by size:
4220 761 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 2137 3065 3158 2137
Size: 928 93 4533
Fragments arranged by size:
4533 928 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2271 2320 2659 2873 3615 3700
Size: 399 365 116 49 339 214 742 85
Cuts at: 3700 4771 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 365 339 214 116
85 49
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2737 2909 844
Size: 1893 172 3489
Fragments arranged by size:
3489 1893 172
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2500 4049 4223 65
Size: 1183 1252 1549 174 1396
Fragments arranged by size:
1549 1396 1252 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 2643 96
Size: 2547 3007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 2007 4358 2007
Size: 2351 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2038 2396 2512 2884 3251 3636 3653 5105
Size: 66 358 116 372 367 385 17 1452
Cuts at: 5105 5191 5203 5549 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 372 367 358 346 116
86 66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2289 2301 2332 2635 513
Size: 552 1224 12 31 303 3432
Fragments arranged by size:
3432 1224 552 303 31 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2557 487
Size: 683 56 1331 3484
Fragments arranged by size:
3484 1331 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2120 2608 2680 3070 3864 3884 2033
Size: 87 488 72 390 794 20 3703
Fragments arranged by size:
3703 794 488 390 87 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2747 4561 809
Size: 507 1431 1814 1802
Fragments arranged by size:
1814 1802 1431 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2430 2650
Size: 54 1425 50 275 18 528 72 220
Cuts at: 2650 2820 3001 3890 5519 8
Size: 170 181 889 1629 43
Fragments arranged by size:
1629 1425 889 528 275 220 181 170
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3484 1353
Size: 2131 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2360 2474 2774 4141 4515 4661
Size: 1064 138 771 114 300 1367 374 146
Cuts at: 4661 387
Size: 1280
Fragments arranged by size:
1367 1280 1064 771 374 300 146 138
114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2537 3617 4214 5001 5269 1239
Size: 393 518 387 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 518 393 387 268
EcoO109I rG'GnC_Cy
Cuts at: 2056 2152 2222 2337 2353 2056
Size: 96 70 115 16 5257
Fragments arranged by size:
5257 115 96 70 16
EcoRI G'AATT_C
Cuts at: 2762 2863 3241 2762
Size: 101 378 5075
Fragments arranged by size:
5075 378 101
FspI TGC'GCA
Cuts at: 2672 4060 2672
Size: 1388 4166
HaeII r_GCGC'y
Cuts at: 1678 2537 4701 1678
Size: 859 2164 2531
Fragments arranged by size:
2531 2164 859
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 4195 4227 4269
Size: 32 135 32 925 32 1666 32 42
Cuts at: 4269 4301 1373
Size: 32 2626
Fragments arranged by size:
2626 1666 925 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 2432 2822 10
Size: 54 1425 343 528 72 390 2742
Fragments arranged by size:
2742 1425 528 390 343 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3512 3871 4030 518
Size: 648 2346 359 159 2042
Fragments arranged by size:
2346 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2259 2334 2502 2579 2637 2781 2845
Size: 542 111 75 168 77 58 144 64
Cuts at: 2845 2970 3647 4795 5040 1606
Size: 125 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 542 245 168 144 125
111 77 75 64 58
NcoI C'CATG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 3766
Fragments arranged by size:
3766 1236 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 2838 1108
Size: 1248 482 3824
Fragments arranged by size:
3824 1248 482
PfoI T'CCnGG_A
Cuts at: 2712 2766 2712
Size: 54 5500
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PspOMI G'GGCC_C
Cuts at: 2151 2151
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2148 2259 2579 2845 2148
Size: 111 320 266 4857
Fragments arranged by size:
4857 320 266 111
SacII CC_GC'GG
Cuts at: 2335 2638 2335
Size: 303 5251
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2776 2902 4037 4718 4909 826
Size: 219 1731 126 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2561 2881 1872
Size: 689 320 4545
Fragments arranged by size:
4545 689 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2851 3345 493
Size: 2358 494 2702
Fragments arranged by size:
2702 2358 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2301 2664 2993 513
Size: 552 1236 363 329 3074
Fragments arranged by size:
3074 1236 552 363 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 2702 3581 3598 3751 3936 4381
Size: 1255 535 587 879 17 153 185 445
Cuts at: 4381 325
Size: 1498
Fragments arranged by size:
1498 1255 879 587 535 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 3800 5512
Size: 274 80 33 51 1443 666 1157 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1443 1157 666 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 5514 5546 2148
Size: 32 3334 32 2156
Fragments arranged by size:
3334 2156 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2612 1316
Size: 1296 4258
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2440 2278
Size: 162 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2559 2879 1870
Size: 689 320 4545
Fragments arranged by size:
4545 689 320
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI