Restriction map for
pFN21ASDF0187
(Circular) (Six-base) MAPSORT of: pFN21ASDF0187.seq Check: 99 from: 1 to: 6418
pFN21ASDF0187 6418 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3761 1937
Size: 1824 4594
Acc65I G'GTAC_C
Cuts at: 3739 3739
Size: 6418
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 2273 3079 3144 3247 3331 3415 4481
Size: 550 91 806 65 103 84 84 1066
Cuts at: 4481 5865 6133 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1066 806 550 268 103 91
84 84 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6221
AflIII A'CryG_T
Cuts at: 1203 2130 5317 6294 1203
Size: 927 3187 977 1327
Fragments arranged by size:
3187 1327 977 927
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 2447 5147 2447
Size: 2700 3718
AleI CACnn'nnGTG
Cuts at: 1166 3156 1166
Size: 1990 4428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6418
AlwNI CAG_nnn'CTG
Cuts at: 2275 2308 2758 4161 5733 2275
Size: 33 450 1403 1572 2960
Fragments arranged by size:
2960 1572 1403 450 33
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 6418
ApaLI G'TGCA_C
Cuts at: 3577 4475 5631 3577
Size: 898 1156 4364
Fragments arranged by size:
4364 1156 898
ApoI r'AATT_y
Cuts at: 1493 3465 3549 3727 3953 4105 6214 1493
Size: 1972 84 178 226 152 2109 1697
Fragments arranged by size:
2109 1972 1697 226 178 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2521 3558 3743 1870
Size: 73 289 289 1037 185 4545
Fragments arranged by size:
4545 1037 289 289 185 73
BamHI G'GATC_C
Cuts at: 2503 3297 3748 4115 2503
Size: 794 451 367 4806
Fragments arranged by size:
4806 794 451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 3739 5194 618
Size: 325 217 627 707 1245 1455 1842
Fragments arranged by size:
1842 1455 1245 707 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2358 2605 3107 3443 3737 1391
Size: 841 126 247 502 336 294 4072
Fragments arranged by size:
4072 841 502 336 294 247 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2424 928
Size: 971 231 294 4922
Fragments arranged by size:
4922 971 294 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6418
BfrBI ATG'CAT
Cuts at: 3683 3683
Size: 6418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5029 136
Size: 107 122 71 631 605 3357 1525
Fragments arranged by size:
3357 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6413 6413
Size: 6418
BlpI GC'TnA_GC
Cuts at: 3835 3835
Size: 6418
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3581 4479 5635 1790
Size: 442 1349 898 1156 2573
Fragments arranged by size:
2573 1349 1156 898 442
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 4149 5107
Size: 416 53 83 399 24 1128 1604 958
Cuts at: 5107 442
Size: 1753
Fragments arranged by size:
1753 1604 1128 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2872 3670 2258
Size: 614 798 5006
Fragments arranged by size:
5006 798 614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2330 2342 2756 3243 3411 5078 1239
Size: 915 176 12 414 487 168 1667 2579
Fragments arranged by size:
2579 1667 915 487 414 176 168 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4543 5408 5706 4543
Size: 865 298 5255
Fragments arranged by size:
5255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2646 5081 882
Size: 617 1147 2435 2219
Fragments arranged by size:
2435 2219 1147 617
BsaAI yAC'GTr
Cuts at: 493 1204 3715 4209 493
Size: 711 2511 494 2702
Fragments arranged by size:
2702 2511 711 494
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4225 4607 5242 275
Size: 53 83 186 936 2692 382 635 1451
Fragments arranged by size:
2692 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4851 5249 5523 5670 6081 4851
Size: 398 274 147 411 5188
Fragments arranged by size:
5188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2152 2182 1178
Size: 30 165 30 749 30 5414
Fragments arranged by size:
5414 749 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2157 2411 2861 2996 1186
Size: 971 254 450 135 4608
Fragments arranged by size:
4608 971 450 254 135
BseYI C'CCAG_C
Cuts at: 3667 5621 6117 3667
Size: 1954 496 3968
Fragments arranged by size:
3968 1954 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2055 2610 2655 1163
Size: 573 319 555 45 4926
Fragments arranged by size:
4926 573 555 319 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 2605 3107 3443 3581 4479 4564 5635 2358
Size: 247 502 336 138 898 85 1071 3141
Fragments arranged by size:
3141 1071 898 502 336 247 138 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
BsmBI CGTCTCn'nnnn_
Cuts at: 2577 2577
Size: 6418
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2358 2605 3107 3443 3581 3737
Size: 399 442 126 247 502 336 138 156
Cuts at: 3737 4479 4564 5635 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 442 399 336 247
156 138 126 85
BspEI T'CCGG_A
Cuts at: 6081 6081
Size: 6418
BspHI T'CATG_A
Cuts at: 2635 3319 4307 6037 2635
Size: 684 988 1730 3016
Fragments arranged by size:
3016 1730 988 684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3773 844
Size: 2929 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2703 4913 5087 65
Size: 1183 1455 2210 174 1396
Fragments arranged by size:
2210 1455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5062 5249 1700
Size: 110 3252 187 2869
Fragments arranged by size:
3252 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 2540 2598 3181 4478 5490 2540
Size: 58 583 1297 1012 3468
Fragments arranged by size:
3468 1297 1012 583 58
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6418
BstBI TT'CG_AA
Cuts at: 2007 5222 2007
Size: 3215 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2304 2503 2909 3297 3748 4115 4500 4517
Size: 332 199 406 388 451 367 385 17
Cuts at: 4517 5969 6055 6067 6413 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 451 406 388 385 367 346
332 199 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 6418
BtgI C'CryG_G
Cuts at: 513 1065 2049 3135 513
Size: 552 984 1086 3796
Fragments arranged by size:
3796 1086 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2675 487
Size: 683 56 1449 4230
Fragments arranged by size:
4230 1449 683 56
BtsI GCAGTG_nn'
Cuts at: 3934 4728 4748 3934
Size: 794 20 5604
Fragments arranged by size:
5604 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DraIII CAC_nnn'GTG
Cuts at: 3585 3585
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2279 3865 4754
Size: 54 1425 50 275 18 449 1586 889
Cuts at: 4754 6383 8
Size: 1629 43
Fragments arranged by size:
1629 1586 1425 889 449 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2610 4348 1353
Size: 750 507 1738 3423
Fragments arranged by size:
3423 1738 750 507
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2177 3191 5005 5379 5525 387
Size: 1064 138 588 1014 1814 374 146 1280
Fragments arranged by size:
1814 1280 1064 1014 588 374 146 138
EcoICRI GAG'CTC
Cuts at: 2356 2603 3105 3441 2356
Size: 247 502 336 5333
Fragments arranged by size:
5333 502 336 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2182 2273 2330 2342 2756 3079
Size: 393 522 28 91 57 12 414 323
Cuts at: 3079 3144 3243 3247 3331 3411 3415 4481 5078
Size: 65 99 4 84 80 4 1066 597
Cuts at: 5078 5865 6133 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1066 787 597 522 414 393 323
268 99 91 84 80 65 57 28
12 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 2348
Size: 6418
EcoO109I rG'GnC_Cy
Cuts at: 2229 2449 2229
Size: 220 6198
EcoRI G'AATT_C
Cuts at: 3465 3549 3727 4105 3465
Size: 84 178 378 5778
Fragments arranged by size:
5778 378 178 84
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 2206 5565 1678
Size: 528 3359 2531
Fragments arranged by size:
3359 2531 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2433 2465 2482 2514 2870
Size: 32 135 32 861 32 17 32 356
Cuts at: 2870 2902 3526 3558 5059 5091 5133 5165 1373
Size: 32 624 32 1501 32 42 32 2626
Fragments arranged by size:
2626 1501 861 624 356 135 42 32
32 32 32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3762 4008 669
Size: 988 281 915 909 246 3079
Fragments arranged by size:
3079 988 915 909 281 246
HindIII A'AGCT_T
Cuts at: 748 3091 3510 748
Size: 2343 419 3656
Fragments arranged by size:
3656 2343 419
HpaI GTT'AAC
Cuts at: 4008 4008
Size: 6418
KpnI G_GTAC'C
Cuts at: 3743 3743
Size: 6418
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 3300 5532 5716 1305
Size: 1827 168 2232 184 2007
Fragments arranged by size:
2232 2007 1827 184 168
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4596
Fragments arranged by size:
4596 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3156 4376 4735 4894 518
Size: 648 1554 436 1220 359 159 2042
Fragments arranged by size:
2042 1554 1220 648 436 359 159
MspA1I CmG'CkG
Cuts at: 1606 2684 3834 4511 5659 5904 1606
Size: 1078 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 1078 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5866
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NsiI A_TGCA'T
Cuts at: 3685 3685
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 2134 3776 5321 1132
Size: 97 905 1642 1545 2229
Fragments arranged by size:
2229 1642 1545 905 97
PasI CC'CwG_GG
Cuts at: 1348 2818 1348
Size: 1470 4948
PciI A'CATG_T
Cuts at: 2130 5317 2130
Size: 3187 3231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 2698 1108
Size: 1554 36 4828
Fragments arranged by size:
4828 1554 36
PfoI T'CCnGG_A
Cuts at: 2307 2307
Size: 6418
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3165 4487 4519 6000 6032 3133
Size: 32 1322 32 1481 32 3519
Fragments arranged by size:
3519 1481 1322 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2449
Size: 6418
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6418
PsiI TTA'TAA
Cuts at: 3988 3988
Size: 6418
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 6418
PspXI vC'TCGA_Gb
Cuts at: 2521 2521
Size: 6418
PstI C_TGCA'G
Cuts at: 830 2373 3770 830
Size: 1543 1397 3478
Fragments arranged by size:
3478 1543 1397
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
SacI G_AGCT'C
Cuts at: 2358 2605 3107 3443 2358
Size: 247 502 336 5333
Fragments arranged by size:
5333 502 336 247
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SapI GCTCTTCn'nnn_
Cuts at: 2610 2610
Size: 6418
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SfcI C'TryA_G
Cuts at: 826 1045 2153 2369 3021 3766 4901 5582 5773
Size: 219 1108 216 652 745 1135 681 191
Cuts at: 5773 826
Size: 1471
Fragments arranged by size:
1471 1135 1108 745 681 652 219 216
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2521 3558 4522 5423 5685 820
Size: 197 926 578 1037 964 901 262 1553
Fragments arranged by size:
1553 1037 964 926 901 578 262 197
SnaBI TAC'GTA
Cuts at: 493 3715 4209 493
Size: 3222 494 2702
Fragments arranged by size:
3222 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 4342 5
Size: 47 4290 2081
Fragments arranged by size:
4290 2081 47
StuI AGG'CCT
Cuts at: 1517 3344 3428 3596 1517
Size: 1827 84 168 4339
Fragments arranged by size:
4339 1827 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2295 2349 3062 3857 513
Size: 552 1230 54 713 795 3074
Fragments arranged by size:
3074 1230 795 713 552 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 4445 4462 4615 4800 5245 325
Size: 1255 984 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 984 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3660 4664 6376
Size: 274 80 33 51 1443 1683 1004 1712
Cuts at: 6376 96
Size: 138
Fragments arranged by size:
1712 1683 1443 1004 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 6378 6410 2152
Size: 32 4194 32 2160
Fragments arranged by size:
4194 2160 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 2413 3754 2413
Size: 1341 5077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2921 2222
Size: 699 5719
XhoI C'TCGA_G
Cuts at: 1943 2521 3558 1943
Size: 578 1037 4803
Fragments arranged by size:
4803 1037 578
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 3129 3260 3428 4547 3129
Size: 131 168 1119 5000
Fragments arranged by size:
5000 1119 168 131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI