Restriction map for
pFN21ASDF0168
(Circular) (Six-base) MAPSORT of: pFN21ASDF0168.seq Check: 1211 from: 1 to: 6430
pFN21ASDF0168 6430 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2972 2981 4493 5877 6145 1632
Size: 581 759 9 1512 1384 268 1917
Fragments arranged by size:
1917 1512 1384 759 581 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 2454 3216 5329 6306 1203
Size: 1251 762 2113 977 1327
Fragments arranged by size:
2113 1327 1251 977 762
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 2273 2719 5159 2273
Size: 446 2440 3544
Fragments arranged by size:
3544 2440 446
AleI CACnn'nnGTG
Cuts at: 1166 3083 1166
Size: 1917 4513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2632 1184
Size: 1448 4982
AlwNI CAG_nnn'CTG
Cuts at: 2413 2707 4173 5745 2413
Size: 294 1466 1572 3098
Fragments arranged by size:
3098 1572 1466 294
ApaI G_GGCC'C
Cuts at: 2770 2770
Size: 6430
ApaLI G'TGCA_C
Cuts at: 2468 2912 4487 5643 2468
Size: 444 1575 1156 3255
Fragments arranged by size:
3255 1575 1156 444
ApoI r'AATT_y
Cuts at: 1493 3739 3965 4117 6226 1493
Size: 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 2669 3572 3755 1870
Size: 73 567 159 903 183 4545
Fragments arranged by size:
4545 903 567 183 159 73
BamHI G'GATC_C
Cuts at: 2137 2388 3760 4127 2137
Size: 251 1372 367 4440
Fragments arranged by size:
4440 1372 367 251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2506 3332 3751 5206 618
Size: 325 217 627 719 826 419 1455 1842
Fragments arranged by size:
1842 1455 826 719 627 419 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2421 2770 2849 3749 1391
Size: 790 240 349 79 900 4072
Fragments arranged by size:
4072 900 790 349 240 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2568 928
Size: 971 669 4790
Fragments arranged by size:
4790 971 669
BbvCI CC'TCA_GC
Cuts at: 2069 2780 2069
Size: 711 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3322 3356 4621 4655 1903
Size: 34 1385 34 1265 34 3678
Fragments arranged by size:
3678 1385 1265 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BfrBI ATG'CAT
Cuts at: 2130 2130
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2765 3112
Size: 107 122 71 631 605 361 732 347
Cuts at: 3112 3378 5041 136
Size: 266 1663 1525
Fragments arranged by size:
1663 1525 732 631 605 361 347 266
122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2255 2843 3847 2255
Size: 588 1004 4838
Fragments arranged by size:
4838 1004 588
Bme1580I G_kGCm'C
Cuts at: 1790 2472 2511 2770 2916 3582 4491 5647 1790
Size: 682 39 259 146 666 909 1156 2573
Fragments arranged by size:
2573 1156 909 682 666 259 146 39
BmgBI CAC'GTC
Cuts at: 1318 2716 2971 3289 1318
Size: 1398 255 318 4459
Fragments arranged by size:
4459 1398 318 255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 2621 4161
Size: 416 53 83 399 24 1017 187 1540
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1540 1017 958 416 399 187 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2777 3654 5090 1239
Size: 1067 471 877 1436 2579
Fragments arranged by size:
2579 1436 1067 877 471
Bpu10I CC'TnA_GC
Cuts at: 2069 2684 2780 3092 2069
Size: 615 96 312 5407
Fragments arranged by size:
5407 615 312 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2588 3271 4555 5420 5718 2144
Size: 444 683 1284 865 298 2856
Fragments arranged by size:
2856 1284 865 683 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2921 5093 882
Size: 617 1422 2172 2219
Fragments arranged by size:
2219 2172 1422 617
BsaAI yAC'GTr
Cuts at: 493 1204 2457 2917 3070 3219 3727 4221 493
Size: 711 1253 460 153 149 508 494 2702
Fragments arranged by size:
2702 1253 711 508 494 460 153 149
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3339 4237 4619 5254
Size: 53 83 186 936 1806 898 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
1806 1451 936 898 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6205
Fragments arranged by size:
6205 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2057 3134 1186
Size: 850 21 1077 4482
Fragments arranged by size:
4482 1077 850 21
BseYI C'CCAG_C
Cuts at: 2356 2375 2518 2817 3486 5633 6129 2356
Size: 19 143 299 669 2147 496 2657
Fragments arranged by size:
2657 2147 669 496 299 143 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2720 2939 3047 1163
Size: 573 288 696 219 108 4546
Fragments arranged by size:
4546 696 573 288 219 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2693 2697 3154 3880 4641 5669
Size: 275 186 692 4 457 726 761 1028
Cuts at: 5669 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 726 692 457 275 186
4
BsiHKAI G_wGCw'C
Cuts at: 2256 2472 2849 2916 3126 3468 4491 4576 5647
Size: 216 377 67 210 342 1023 85 1071
Cuts at: 5647 2256
Size: 3039
Fragments arranged by size:
3039 1071 1023 377 342 216 210 85
67
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
BsmBI CGTCTCn'nnnn_
Cuts at: 2706 3488 2706
Size: 782 5648
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2256 2421 2472 2511 2770 2849
Size: 399 391 75 165 51 39 259 79
Cuts at: 2849 2916 3126 3468 3582 3749 4491 4576 5647
Size: 67 210 342 114 167 742 85 1071
Cuts at: 5647 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 391 342 259 210
167 165 114 85 79 75 67 51
39
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2832 3785 844
Size: 1988 953 3489
Fragments arranged by size:
3489 1988 953
BsrBI CCG'CTC
Cuts at: 1394 1823 2991 4317 1394
Size: 429 1168 1326 3507
Fragments arranged by size:
3507 1326 1168 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3296 4925 5099 65
Size: 1183 2048 1629 174 1396
Fragments arranged by size:
2048 1629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2688 2692 2995 3073 3245 3335 3397
Size: 110 878 4 303 78 172 90 62
Cuts at: 3397 3458 5074 5261 1700
Size: 61 1616 187 2869
Fragments arranged by size:
2869 1616 878 303 187 172 110 90
78 62 61 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 2833 2835 3195 3517 1925
Size: 908 2 360 322 4838
Fragments arranged by size:
4838 908 360 322 2
BssSI C'ACGA_G
Cuts at: 3709 4490 5502 3709
Size: 781 1012 4637
Fragments arranged by size:
4637 1012 781
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 3070 794
Size: 2117 159 4154
Fragments arranged by size:
4154 2117 159
BstBI TT'CG_AA
Cuts at: 2007 5234 2007
Size: 3227 3203
BstEII G'GTnAC_C
Cuts at: 1184 2167 2275 1184
Size: 983 108 5339
Fragments arranged by size:
5339 983 108
BstYI r'GATC_y
Cuts at: 1972 2137 2388 3760 4127 4512 4529 5981 6067
Size: 165 251 1372 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1372 385 367 346 251 165
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2880 513
Size: 552 957 858 4063
Fragments arranged by size:
4063 957 858 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2274 2642 2903 487
Size: 683 56 1048 368 261 4014
Fragments arranged by size:
4014 1048 683 368 261 56
BtsI GCAGTG_nn'
Cuts at: 3946 4740 4760 3946
Size: 794 20 5616
Fragments arranged by size:
5616 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 2476 2911 3070 2476
Size: 435 159 5836
Fragments arranged by size:
5836 435 159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3431 5437 809
Size: 507 2115 2006 1802
Fragments arranged by size:
2115 2006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2690
Size: 54 1425 50 275 18 399 12 449
Cuts at: 2690 2694 3075 3136 3442 3564 3621 3877 4766
Size: 4 381 61 306 122 57 256 889
Cuts at: 4766 6395 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 449 399 381 306 275
256 122 61 57 54 50 43 18
12 4
EagI C'GGCC_G
Cuts at: 1537 1812 2690 2694 3877 1537
Size: 275 878 4 1183 4090
Fragments arranged by size:
4090 1183 878 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3255 4360 1353
Size: 1902 1105 3423
Fragments arranged by size:
3423 1902 1105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3371 5017 5391 5537 387
Size: 1064 138 1782 1646 374 146 1280
Fragments arranged by size:
1782 1646 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2847 2847
Size: 6430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2306 2777 2972 2981 3654 4493
Size: 393 581 93 471 195 9 673 839
Cuts at: 4493 5090 5877 6145 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 839 787 673 597 581 471 393
268 195 93 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3647 3647
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2410 2590 2758 2821 3105 3548 3714 2410
Size: 180 168 63 284 443 166 5126
Fragments arranged by size:
5126 443 284 180 168 166 63
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FseI GG_CCGG'CC
Cuts at: 2696 2696
Size: 6430
FspI TGC'GCA
Cuts at: 2369 2486 4936 2369
Size: 117 2450 3863
Fragments arranged by size:
3863 2450 117
FspAI rTGC'GCAy
Cuts at: 2369 2369
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2223 3053 3281 3404 3515 5577 1678
Size: 545 830 228 123 111 2062 2531
Fragments arranged by size:
2531 2062 830 545 228 123 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 2705 2737 3143
Size: 32 135 32 687 32 414 32 406
Cuts at: 3143 3175 5071 5103 5145 5177 1373
Size: 32 1896 32 42 32 2626
Fragments arranged by size:
2626 1896 687 414 406 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3094 748
Size: 2346 4084
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3204 5544 5728 1305
Size: 1899 2340 184 2007
Fragments arranged by size:
2340 2007 1899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3623 10
Size: 54 1425 343 1791 2817
Fragments arranged by size:
2817 1791 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 3083 3612 4388 4747 4906 518
Size: 648 1203 714 529 776 359 159 2042
Fragments arranged by size:
2042 1203 776 714 648 529 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2775 3028 3846 4523 5671 5916 1606
Size: 549 620 253 818 677 1148 245 2120
Fragments arranged by size:
2120 1148 818 677 620 549 253 245
NaeI GCC'GGC
Cuts at: 2690 2694 3075 3247 3399 3460 2690
Size: 4 381 172 152 61 5660
Fragments arranged by size:
5660 381 172 152 61 4
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4921
Fragments arranged by size:
4921 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2688 2692 3073 3245 3397 3458 2688
Size: 4 381 172 152 61 5660
Fragments arranged by size:
5660 381 172 152 61 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NsiI A_TGCA'T
Cuts at: 2132 2132
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2134 2374 3043 3611 3788 5333 1132
Size: 97 905 240 669 568 177 1545 2229
Fragments arranged by size:
2229 1545 905 669 568 240 177 97
PasI CC'CwG_GG
Cuts at: 1348 2896 1348
Size: 1548 4882
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 1108
Size: 1305 5125
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PmlI CAC'GTG
Cuts at: 2457 2917 3219 2457
Size: 460 302 5668
Fragments arranged by size:
5668 460 302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 3714 2821
Size: 893 5537
PshAI GACnn'nnGTC
Cuts at: 1613 3500 1613
Size: 1887 4543
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2766 2766
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2621 3782 830
Size: 1791 1161 3478
Fragments arranged by size:
3478 1791 1161
PvuI CG_AT'CG
Cuts at: 2001 3154 2001
Size: 1153 5277
PvuII CAG'CTG
Cuts at: 2155 2775 2155
Size: 620 5810
RsrII CG'GwC_CG
Cuts at: 2993 2993
Size: 6430
SacI G_AGCT'C
Cuts at: 2849 2849
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2617 3778 4913 5594 5785 826
Size: 219 1572 1161 1135 681 191 1471
Fragments arranged by size:
1572 1471 1161 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2033 2765 2033
Size: 732 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 3335 3397 1700
Size: 1635 62 4733
Fragments arranged by size:
4733 1635 62
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2603 3250 4534 5435 5697
Size: 197 926 216 444 647 1284 901 262
Cuts at: 5697 820
Size: 1553
Fragments arranged by size:
1553 1284 926 901 647 444 262 216
197
SnaBI TAC'GTA
Cuts at: 493 3727 4221 493
Size: 3234 494 2702
Fragments arranged by size:
3234 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 2134 2374 3788 1132
Size: 1002 240 1414 3774
Fragments arranged by size:
3774 1414 1002 240
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2022 2247 3081 3676 3869 513
Size: 552 957 225 834 595 193 3074
Fragments arranged by size:
3074 957 834 595 552 225 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2801 3478 4457 4474 4627 4812
Size: 1255 873 348 677 979 17 153 185
Cuts at: 4812 5257 325
Size: 445 1498
Fragments arranged by size:
1498 1255 979 873 677 445 348 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 4676 6388
Size: 274 80 33 51 1443 819 1880 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1880 1712 1443 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2985 3017 6390 6422 2985
Size: 32 3373 32 2993
Fragments arranged by size:
3373 2993 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 2664 4559 2664
Size: 1895 4535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I EcoRV FalI KasI NarI
NruI PacI PspXI PsrI SacII SanDI SapI SexAI
SfoI SrfI SwaI