Restriction map for
pFN21ASDF0136
(Circular) (Six-base) MAPSORT of: pFN21ASDF0136.seq Check: 7252 from: 1 to: 7144
pFN21ASDF0136 7144 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3092 4033 4070 3092
Size: 941 37 6166
Fragments arranged by size:
6166 941 37
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4954 278
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4487 1937
Size: 2550 4594
Acc65I G'GTAC_C
Cuts at: 4170 4465 4170
Size: 295 6849
AclI AA'CG_TT
Cuts at: 1563 5271 5644 1563
Size: 3708 373 3063
Fragments arranged by size:
3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2744 3266 3779 5207 6591 6859 1632
Size: 1112 522 513 1428 1384 268 1917
Fragments arranged by size:
1917 1428 1384 1112 522 513 268
AflII C'TTAA_G
Cuts at: 820 1017 2530 820
Size: 197 1513 5434
Fragments arranged by size:
5434 1513 197
AflIII A'CryG_T
Cuts at: 1203 6043 7020 1203
Size: 4840 977 1327
Fragments arranged by size:
4840 1327 977
AgeI A'CCGG_T
Cuts at: 3672 4167 5975 3672
Size: 495 1808 4841
Fragments arranged by size:
4841 1808 495
AhdI GACnn_n'nnGTC
Cuts at: 5873 5873
Size: 7144
AleI CACnn'nnGTG
Cuts at: 1166 3095 1166
Size: 1929 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7144
AlwNI CAG_nnn'CTG
Cuts at: 2035 2488 2701 3416 3667 3826 4887 6459 2035
Size: 453 213 715 251 159 1061 1572 2720
Fragments arranged by size:
2720 1572 1061 715 453 251 213 159
ApaI G_GGCC'C
Cuts at: 2087 2840 2873 3481 2087
Size: 753 33 608 5750
Fragments arranged by size:
5750 753 608 33
ApaLI G'TGCA_C
Cuts at: 2111 2152 3004 4163 5201 6357 2111
Size: 41 852 1159 1038 1156 2898
Fragments arranged by size:
2898 1159 1156 1038 852 41
ApoI r'AATT_y
Cuts at: 1493 4453 4679 4831 6940 1493
Size: 2960 226 152 2109 1697
Fragments arranged by size:
2960 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5698 160
Size: 872 4666 1606
Fragments arranged by size:
4666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2372 2575 4054 4469 1870
Size: 73 288 141 203 1479 415 4545
Fragments arranged by size:
4545 1479 415 288 203 141 73
BamHI G'GATC_C
Cuts at: 4474 4841 4474
Size: 367 6777
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 2306 3934 4010 4170
Size: 325 217 627 450 69 1628 76 160
Cuts at: 4170 4402 4465 5920 618
Size: 232 63 1455 1842
Fragments arranged by size:
1842 1628 1455 627 450 325 232 217
160 76 69 63
BanII G_rGCy'C
Cuts at: 1391 2087 2481 2840 2873 2901 3481 4463 1391
Size: 696 394 359 33 28 580 982 4072
Fragments arranged by size:
4072 982 696 580 394 359 33 28
BbeI G_GCGC'C
Cuts at: 3938 4014 3938
Size: 76 7068
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3163 3376 3886 3914 3967 4012 4189
Size: 971 1264 213 510 28 53 45 177
Cuts at: 4189 928
Size: 3883
Fragments arranged by size:
3883 1264 971 510 213 177 53 45
28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4379 4413 5335 5369 1903
Size: 34 2442 34 922 34 3678
Fragments arranged by size:
3678 2442 922 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5036 6246 5036
Size: 1210 5934
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2601 2696 2745
Size: 107 122 71 631 605 929 95 49
Cuts at: 2745 3943 5755 136
Size: 1198 1812 1525
Fragments arranged by size:
1812 1525 1198 929 631 605 122 107
95 71 49
BglII A'GATC_T
Cuts at: 7139 7139
Size: 7144
BlpI GC'TnA_GC
Cuts at: 4561 4561
Size: 7144
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2115 2156 2240 2840 2873 3008 3481
Size: 297 28 41 84 600 33 135 473
Cuts at: 3481 4167 5205 6361 1790
Size: 686 1038 1156 2573
Fragments arranged by size:
2573 1156 1038 686 600 473 297 135
84 41 33 28
BmgBI CAC'GTC
Cuts at: 1318 3382 1318
Size: 2064 5080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 4875 5833
Size: 416 53 83 399 24 897 2561 958
Cuts at: 5833 442
Size: 1753
Fragments arranged by size:
2561 1753 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2441 2462 2465 2734 2915 3203 3530
Size: 953 249 21 3 269 181 288 327
Cuts at: 3530 3554 3824 4145 5804 1239
Size: 24 270 321 1659 2579
Fragments arranged by size:
2579 1659 953 327 321 288 270 269
249 181 24 21 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 3554 4265 5269 6134 6432 2762
Size: 792 711 1004 865 298 3474
Fragments arranged by size:
3474 1004 865 792 711 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4150 5807 882
Size: 617 555 2096 1657 2219
Fragments arranged by size:
2219 2096 1657 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3544 4441 4935 493
Size: 711 2340 897 494 2702
Fragments arranged by size:
2702 2340 897 711 494
BsaBI GATnn'nnATC
Cuts at: 7138 7138
Size: 7144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3624 3935 4011 4951
Size: 53 83 186 936 2091 311 76 940
Cuts at: 4951 5333 5968 275
Size: 382 635 1451
Fragments arranged by size:
2091 1451 940 936 635 382 311 186
83 76 53
BsaWI w'CCGG_w
Cuts at: 3672 4074 4167 5577 5975 6249 6396 6807 3672
Size: 402 93 1410 398 274 147 411 4009
Fragments arranged by size:
4009 1410 411 402 398 274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6919
Fragments arranged by size:
6919 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2462 2465 2891 3455 3778 3848 4379
Size: 1273 3 3 426 564 323 70 531
Cuts at: 4379 4384 4406 1186
Size: 5 22 3924
Fragments arranged by size:
3924 1273 564 531 426 323 70 22
5 3 3
BseYI C'CCAG_C
Cuts at: 2028 2087 2279 2399 3322 3358 3607 3642 3835
Size: 59 192 120 923 36 249 35 193
Cuts at: 3835 6347 6843 2028
Size: 2512 496 2329
Fragments arranged by size:
2512 2329 923 496 249 193 192 120
59 36 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2371 2869 3890 1163
Size: 573 624 11 498 1021 4417
Fragments arranged by size:
4417 1021 624 573 498 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4594 5355 6383 1540
Size: 275 186 2593 761 1028 2301
Fragments arranged by size:
2593 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2156 2249 2481 2760 2901 3008 3846 3971
Size: 41 93 232 279 141 107 838 125
Cuts at: 3971 4061 4167 5205 5290 6361 2115
Size: 90 106 1038 85 1071 2898
Fragments arranged by size:
2898 1071 1038 838 279 232 141 125
107 106 93 90 85 41
BsmI GAATG_Cn'
Cuts at: 4655 4748 4655
Size: 93 7051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2115 2156 2240 2249 2481 2760
Size: 399 297 28 41 84 9 232 279
Cuts at: 2760 2840 2873 2901 3008 3481 3846 3971 4061
Size: 80 33 28 107 473 365 125 90
Cuts at: 4061 4167 4463 5205 5290 6361 1391
Size: 106 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 399 365 297 296
279 232 125 107 106 90 85 84
80 41 33 28 28 9
BspEI T'CCGG_A
Cuts at: 4074 6807 4074
Size: 2733 4411
BspHI T'CATG_A
Cuts at: 3396 5033 6763 3396
Size: 1637 1730 3777
Fragments arranged by size:
3777 1730 1637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3092 4033 4070 4499 844
Size: 2248 941 37 429 3489
Fragments arranged by size:
3489 2248 941 429 37
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 2388 5031 1394
Size: 429 368 197 2643 3507
Fragments arranged by size:
3507 2643 429 368 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 3786 4303 5639 5813 65
Size: 1183 2538 517 1336 174 1396
Fragments arranged by size:
2538 1396 1336 1183 517 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2361 2631 3672 3942 4100 4167 5788
Size: 110 551 270 1041 270 158 67 1621
Cuts at: 5788 5975 1700
Size: 187 2869
Fragments arranged by size:
2869 1621 1041 551 270 270 187 158
110 67
BsrGI T'GTAC_A
Cuts at: 96 3099 96
Size: 3003 4141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7144
BssSI C'ACGA_G
Cuts at: 5204 6216 5204
Size: 1012 6132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3003 4409 794
Size: 2209 1406 3529
Fragments arranged by size:
3529 2209 1406
BstBI TT'CG_AA
Cuts at: 2007 5948 2007
Size: 3941 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7144
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2978 3919 2440
Size: 538 941 5665
Fragments arranged by size:
5665 941 538
BstYI r'GATC_y
Cuts at: 1972 4474 4841 5226 5243 6695 6781 6793 7139
Size: 2502 367 385 17 1452 86 12 346
Cuts at: 7139 1972
Size: 1977
Fragments arranged by size:
2502 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2841 2841
Size: 7144
BtgI C'CryG_G
Cuts at: 513 1065 2022 2433 2708 3369 513
Size: 552 957 411 275 661 4288
Fragments arranged by size:
4288 957 661 552 411 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6405
Fragments arranged by size:
6405 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 4660 5454 5474 2499
Size: 2161 794 20 4169
Fragments arranged by size:
4169 2161 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7109
DraI TTT'AAA
Cuts at: 4448 4448
Size: 7144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6151 809
Size: 507 4835 1802
Fragments arranged by size:
4835 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2268 2293
Size: 54 1425 50 275 18 420 18 25
Cuts at: 2293 2629 2944 3258 3372 3421 3501 3546 3576
Size: 336 315 314 114 49 80 45 30
Cuts at: 3576 3921 4221 4591 5480 7109 8
Size: 345 300 370 889 1629 43
Fragments arranged by size:
1629 1425 889 420 370 345 336 315
314 300 275 114 80 54 50 49
45 43 30 25 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4591 1537
Size: 275 2779 4090
Fragments arranged by size:
4090 2779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2558 3755 3851 5074 1353
Size: 1205 1197 96 1223 3423
Fragments arranged by size:
3423 1223 1205 1197 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 2396 2677 3784 5731 6105
Size: 1064 138 791 16 281 1107 1947 374
Cuts at: 6105 6251 387
Size: 146 1280
Fragments arranged by size:
1947 1280 1107 1064 791 374 281 146
138 16
EcoICRI GAG'CTC
Cuts at: 2479 2899 2479
Size: 420 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2441 2462 2465 2734 2744 2915
Size: 393 560 249 21 3 269 10 171
Cuts at: 2915 3203 3266 3530 3554 3779 3824 4145 5207
Size: 288 63 264 24 225 45 321 1062
Cuts at: 5207 5804 6591 6859 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1062 787 597 560 393 321 288
269 268 264 249 225 171 63 45
24 21 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 2471 3221 4280 2444
Size: 27 750 1059 5308
Fragments arranged by size:
5308 1059 750 27
EcoO109I rG'GnC_Cy
Cuts at: 2083 2084 2571 2698 2837 2870 3477 3478 4136
Size: 1 487 127 139 33 607 1 658
Cuts at: 4136 2083
Size: 5091
Fragments arranged by size:
5091 658 607 487 139 127 33 1
1
EcoRI G'AATT_C
Cuts at: 4453 4831 4453
Size: 378 6766
FspI TGC'GCA
Cuts at: 5650 5650
Size: 7144
HaeII r_GCGC'y
Cuts at: 1678 2681 2716 3938 4014 6291 1678
Size: 1003 35 1222 76 2277 2531
Fragments arranged by size:
2531 2277 1222 1003 76 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 3391 3423 5785
Size: 32 135 32 723 32 1064 32 2362
Cuts at: 5785 5817 5859 5891 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2362 1064 723 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4488 4734 669
Size: 988 281 2550 246 3079
Fragments arranged by size:
3079 2550 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4331 748
Size: 3583 3561
HpaI GTT'AAC
Cuts at: 4734 4734
Size: 7144
KasI G'GCGC_C
Cuts at: 3934 4010 3934
Size: 76 7068
KpnI G_GTAC'C
Cuts at: 4174 4469 4174
Size: 295 6849
MfeI C'AATT_G
Cuts at: 4743 4743
Size: 7144
MluI A'CGCG_T
Cuts at: 7020 7020
Size: 7144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6258 6442 1305
Size: 4953 184 2007
Fragments arranged by size:
4953 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 3423 3548 3578 3923
Size: 54 1425 343 438 1153 125 30 345
Cuts at: 3923 10
Size: 3231
Fragments arranged by size:
3231 1425 1153 438 345 343 125 54
30
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3095 4244 5102 5461 5620 518
Size: 648 1767 162 1149 858 359 159 2042
Fragments arranged by size:
2042 1767 1149 858 648 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 2503 2607 3271 4016 4560 5237 6385 6630
Size: 897 104 664 745 544 677 1148 245
Cuts at: 6630 1606
Size: 2120
Fragments arranged by size:
2120 1148 897 745 677 664 544 245
104
NaeI GCC'GGC
Cuts at: 2363 2633 3944 4102 2363
Size: 270 1311 158 5405
Fragments arranged by size:
5405 1311 270 158
NarI GG'CG_CC
Cuts at: 3935 4011 3935
Size: 76 7068
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5635
Fragments arranged by size:
5635 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7144
NgoMIV G'CCGG_C
Cuts at: 2361 2631 3942 4100 2361
Size: 270 1311 158 5405
Fragments arranged by size:
5405 1311 270 158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7144
NotI GC'GGCC_GC
Cuts at: 4591 4591
Size: 7144
NspI r_CATG'y
Cuts at: 1132 1229 2278 2515 3040 4502 6047 1132
Size: 97 1049 237 525 1462 1545 2229
Fragments arranged by size:
2229 1545 1462 1049 525 237 97
PasI CC'CwG_GG
Cuts at: 1348 2066 3012 4140 4158 1348
Size: 718 946 1128 18 4334
Fragments arranged by size:
4334 1128 946 718 18
PciI A'CATG_T
Cuts at: 6043 6043
Size: 7144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2035 2488 3607 1108
Size: 927 453 1119 4645
Fragments arranged by size:
4645 1119 927 453
PfoI T'CCnGG_A
Cuts at: 2136 3801 2136
Size: 1665 5479
PmeI GTTT'AAAC
Cuts at: 4448 4448
Size: 7144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5213 5245 6726 6758 5213
Size: 32 1481 32 5599
Fragments arranged by size:
5599 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2571 2571
Size: 7144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7144
PsiI TTA'TAA
Cuts at: 4714 4714
Size: 7144
PspOMI G'GGCC_C
Cuts at: 2083 2836 2869 3477 2083
Size: 753 33 608 5750
Fragments arranged by size:
5750 753 608 33
PspXI vC'TCGA_Gb
Cuts at: 4054 4054
Size: 7144
PstI C_TGCA'G
Cuts at: 830 2181 2697 3557 3633 4496 830
Size: 1351 516 860 76 863 3478
Fragments arranged by size:
3478 1351 863 860 516 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7144
PvuII CAG'CTG
Cuts at: 3271 3271
Size: 7144
SacI G_AGCT'C
Cuts at: 2481 2901 2481
Size: 420 6724
SalI G'TCGA_C
Cuts at: 1936 4486 1936
Size: 2550 4594
SapI GCTCTTCn'nnn_
Cuts at: 2558 3851 2558
Size: 1293 5851
SbfI CC_TGCA'GG
Cuts at: 2697 3633 4496 2697
Size: 936 863 5345
Fragments arranged by size:
5345 936 863
ScaI AGT'ACT
Cuts at: 2324 5392 7104 2324
Size: 3068 1712 2364
Fragments arranged by size:
3068 2364 1712
SfcI C'TryA_G
Cuts at: 826 1045 2177 2693 2950 3553 3629 4492 5627
Size: 219 1132 516 257 603 76 863 1135
Cuts at: 5627 6308 6499 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1132 863 681 603 516 257
219 191 76
SfoI GGC'GCC
Cuts at: 3936 4012 3936
Size: 76 7068
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7144
SmaI CCC'GGG
Cuts at: 1872 4471 1872
Size: 2599 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2530 2777 3569 4054 4280 5248
Size: 197 926 587 247 792 485 226 968
Cuts at: 5248 6149 6411 820
Size: 901 262 1553
Fragments arranged by size:
1553 968 926 901 792 587 485 262
247 226 197
SnaBI TAC'GTA
Cuts at: 493 4441 4935 493
Size: 3948 494 2702
Fragments arranged by size:
3948 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7144
SphI G_CATG'C
Cuts at: 1132 2278 4502 1132
Size: 1146 2224 3774
Fragments arranged by size:
3774 2224 1146
SspI AAT'ATT
Cuts at: 5 52 3232 5068 5
Size: 47 3180 1836 2081
Fragments arranged by size:
3180 2081 1836 47
StuI AGG'CCT
Cuts at: 1517 2892 1517
Size: 1375 5769
StyI C'CwwG_G
Cuts at: 513 1065 2022 4583 513
Size: 552 957 2561 3074
Fragments arranged by size:
3074 2561 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2939 3008 3025 4102 4150 5171
Size: 1255 675 684 69 17 1077 48 1021
Cuts at: 5171 5188 5341 5526 5971 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1077 1021 684 675 445 185
153 69 48 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2937 3099
Size: 274 80 33 51 1443 345 615 162
Cuts at: 3099 5390 7102 96
Size: 2291 1712 138
Fragments arranged by size:
2291 1712 1443 615 345 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 3716 3748 7104 7136 2247
Size: 32 1437 32 3356 32 2255
Fragments arranged by size:
3356 2255 1437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3692 1316
Size: 2376 4768
XbaI T'CTAG_A
Cuts at: 4480 4480
Size: 7144
XhoI C'TCGA_G
Cuts at: 1943 4054 1943
Size: 2111 5033
XmaI C'CCGG_G
Cuts at: 1870 4469 1870
Size: 2599 4545
XmnI GAAnn'nnTTC
Cuts at: 5273 5273
Size: 7144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4952 276
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI