Restriction map for
pFN21ASDF0018
(Circular) (Six-base) MAPSORT of: pFN21ASDF0018.seq Check: 5592 from: 1 to: 8887
pFN21ASDF0018 8887 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2443 3198 2443
Size: 755 8132
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6697 278
Size: 53 83 186 936 5161 2468
Fragments arranged by size:
5161 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4771 6230 1937
Size: 2834 1459 4594
Fragments arranged by size:
4594 2834 1459
Acc65I G'GTAC_C
Cuts at: 5345 6208 5345
Size: 863 8024
AclI AA'CG_TT
Cuts at: 1563 7014 7387 1563
Size: 5451 373 3063
Fragments arranged by size:
5451 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3672 3780 4182 5318 5766 6950 8334 8602
Size: 2040 108 402 1136 448 1184 1384 268
Cuts at: 8602 1632
Size: 1917
Fragments arranged by size:
2040 1917 1384 1184 1136 448 402 268
108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8690
AflIII A'CryG_T
Cuts at: 1203 4642 4676 4774 6177 7786 8763 1203
Size: 3439 34 98 1403 1609 977 1327
Fragments arranged by size:
3439 1609 1403 1327 977 98 34
AgeI A'CCGG_T
Cuts at: 7718 7718
Size: 8887
AhdI GACnn_n'nnGTC
Cuts at: 7616 7616
Size: 8887
AleI CACnn'nnGTG
Cuts at: 1166 5132 1166
Size: 3966 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2904 1184
Size: 1720 7167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2293 2325 2963 2995 2293
Size: 32 638 32 8185
Fragments arranged by size:
8185 638 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2135 2219 2801 3584 3963 5228 5552 5830 6630
Size: 84 582 783 379 1265 324 278 800
Cuts at: 6630 8202 2135
Size: 1572 2820
Fragments arranged by size:
2820 1572 1265 800 783 582 379 324
278 84
ApaI G_GGCC'C
Cuts at: 2226 2414 2795 3438 5222 5291 5837 5923 6151
Size: 188 381 643 1784 69 546 86 228
Cuts at: 6151 2226
Size: 4962
Fragments arranged by size:
4962 1784 643 546 381 228 188 86
69
ApaLI G'TGCA_C
Cuts at: 6944 8100 6944
Size: 1156 7731
ApoI r'AATT_y
Cuts at: 1493 3924 3951 3969 6196 6422 6574 8683 1493
Size: 2431 27 18 2227 226 152 2109 1697
Fragments arranged by size:
2431 2227 2109 1697 226 152 27 18
AseI AT'TA_AT
Cuts at: 160 1032 7441 160
Size: 872 6409 1606
Fragments arranged by size:
6409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8887
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2160 2343 2611 3135 3381 4444
Size: 73 129 88 183 268 524 246 1063
Cuts at: 4444 4467 4530 4563 5934 6212 1870
Size: 23 63 33 1371 278 4545
Fragments arranged by size:
4545 1371 1063 524 278 268 246 183
129 88 73 63 33 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5577 5610 5577
Size: 33 8854
BamHI G'GATC_C
Cuts at: 2209 2301 4503 5278 5388 6217 6584 2209
Size: 92 2202 775 110 829 367 4512
Fragments arranged by size:
4512 2202 829 775 367 110 92
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 3742 4918 5345 6208
Size: 325 217 627 321 1634 1176 427 863
Cuts at: 6208 7663 618
Size: 1455 1842
Fragments arranged by size:
1842 1634 1455 1176 863 627 427 325
321 217
BanII G_rGCy'C
Cuts at: 1391 2038 2177 2201 2226 2259 2414 2508 2618
Size: 647 139 24 25 33 155 94 110
Cuts at: 2618 2795 2946 3351 3438 3876 4444 4626 5171
Size: 177 151 405 87 438 568 182 545
Cuts at: 5171 5222 5291 5427 5837 5923 5942 6151 6160
Size: 51 69 136 410 86 19 209 9
Cuts at: 6160 6206 1391
Size: 46 4072
Fragments arranged by size:
4072 647 568 545 438 410 405 209
182 177 155 151 139 136 110 94
87 86 69 51 46 33 25 24
19 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5772 928
Size: 971 3873 4043
Fragments arranged by size:
4043 3873 971
BbvCI CC'TCA_GC
Cuts at: 4131 4131
Size: 8887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7078 7112 1903
Size: 34 5141 34 3678
Fragments arranged by size:
5141 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6779 7989 6779
Size: 1210 7677
BclI T'GATC_A
Cuts at: 1548 4942 1548
Size: 3394 5493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4337 4761 6155
Size: 107 122 71 631 605 2665 424 1394
Cuts at: 6155 7498 136
Size: 1343 1525
Fragments arranged by size:
2665 1525 1394 1343 631 605 424 122
107 71
BglII A'GATC_T
Cuts at: 8882 8882
Size: 8887
BlpI GC'TnA_GC
Cuts at: 2239 2441 3510 6020 6304 2239
Size: 202 1069 2510 284 4822
Fragments arranged by size:
4822 2510 1069 284 202
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2414 2462 2795 3325 3438 3939 4684
Size: 436 188 48 333 530 113 501 745
Cuts at: 4684 5139 5222 5291 5837 5923 6151 6948 8104
Size: 455 83 69 546 86 228 797 1156
Cuts at: 8104 1790
Size: 2573
Fragments arranged by size:
2573 1156 797 745 546 530 501 455
436 333 228 188 113 86 83 69
48
BmgBI CAC'GTC
Cuts at: 1318 3862 3934 5961 1318
Size: 2544 72 2027 4244
Fragments arranged by size:
4244 2544 2027 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 2811 4059
Size: 416 53 83 399 24 1374 20 1248
Cuts at: 4059 4193 5705 6618 7576 442
Size: 134 1512 913 958 1753
Fragments arranged by size:
1753 1512 1374 1248 958 913 416 399
134 83 53 24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 2230 2351 2531 2885 3098 3179 3224
Size: 975 16 121 180 354 213 81 45
Cuts at: 3224 3540 3623 3848 4122 4415 4854 4898 5064
Size: 316 83 225 274 293 439 44 166
Cuts at: 5064 5839 7547 1239
Size: 775 1708 2579
Fragments arranged by size:
2579 1708 975 775 439 354 316 293
274 225 213 180 166 121 83 81
45 44 16
Bpu10I CC'TnA_GC
Cuts at: 2186 3116 4131 4791 4839 4989 5040 5752 2186
Size: 930 1015 660 48 150 51 712 5321
Fragments arranged by size:
5321 1015 930 712 660 150 51 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2308 2729 7012 7877 8175 2308
Size: 421 4283 865 298 3020
Fragments arranged by size:
4283 3020 865 421 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3132 3375 7550 882
Size: 617 1633 243 4175 2219
Fragments arranged by size:
4175 2219 1633 617 243
BsaAI yAC'GTr
Cuts at: 493 1204 4645 6049 6184 6678 493
Size: 711 3441 1404 135 494 2702
Fragments arranged by size:
3441 2702 1404 711 494 135
BsaBI GATnn'nnATC
Cuts at: 4404 8881 4404
Size: 4477 4410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4463 5792 6694 7076
Size: 53 83 186 936 2930 1329 902 382
Cuts at: 7076 7711 275
Size: 635 1451
Fragments arranged by size:
2930 1451 1329 936 902 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2293 2933 4323 5566 6090 7320 7718 7992 8139
Size: 640 1390 1243 524 1230 398 274 147
Cuts at: 8139 8550 2293
Size: 411 2630
Fragments arranged by size:
2630 1390 1243 1230 640 524 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2600 2627 2630 2657 2963
Size: 30 165 30 1197 27 3 27 306
Cuts at: 2963 2993 3177 3207 3254 3284 1178
Size: 30 184 30 47 30 6781
Fragments arranged by size:
6781 1197 306 184 165 47 30 30
30 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2581 3000 3363 3818 3821 3824 4235 5688
Size: 1395 419 363 455 3 3 411 1453
Cuts at: 5688 1186
Size: 4385
Fragments arranged by size:
4385 1453 1395 455 419 411 363 3
3
BseYI C'CCAG_C
Cuts at: 2685 2920 3782 3902 3956 4581 5392 5985 5991
Size: 235 862 120 54 625 811 593 6
Cuts at: 5991 8090 8586 2685
Size: 2099 496 2986
Fragments arranged by size:
2986 2099 862 811 625 593 496 235
120 54 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 2930 3570 4892 5346 5538 1163
Size: 573 846 348 640 1322 454 192 4512
Fragments arranged by size:
4512 1322 846 640 573 454 348 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6337 7098 8126 1540
Size: 275 186 4336 761 1028 2301
Fragments arranged by size:
4336 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 2192 2508 2618 2946 5427 6948 7033 8104
Size: 15 316 110 328 2481 1521 85 1071
Cuts at: 8104 2177
Size: 2960
Fragments arranged by size:
2960 2481 1521 1071 328 316 110 85
15
BsmI GAATG_Cn'
Cuts at: 2739 3906 4301 6398 6491 2739
Size: 1167 395 2097 93 5135
Fragments arranged by size:
5135 2097 1167 395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2177 2192 2201 2226 2259 2414
Size: 399 248 139 15 9 25 33 155
Cuts at: 2414 2462 2508 2618 2795 2946 3325 3351 3438
Size: 48 46 110 177 151 379 26 87
Cuts at: 3438 3876 3939 4444 4626 4684 5139 5171 5222
Size: 438 63 505 182 58 455 32 51
Cuts at: 5222 5291 5427 5837 5923 5942 6151 6160 6206
Size: 69 136 410 86 19 209 9 46
Cuts at: 6206 6948 7033 8104 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 455 438 410 399
379 248 209 182 177 155 151 139
136 110 87 86 85 69 63 58
51 48 46 46 33 32 26 25
19 15 9 9
BspEI T'CCGG_A
Cuts at: 4323 5566 8550 4323
Size: 1243 2984 4660
Fragments arranged by size:
4660 2984 1243
BspHI T'CATG_A
Cuts at: 6776 8506 6776
Size: 1730 7157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2443 3198 6242 844
Size: 1599 755 3044 3489
Fragments arranged by size:
3489 3044 1599 755
BsrBI CCG'CTC
Cuts at: 1394 1823 3025 4308 4428 4501 6774 1394
Size: 429 1202 1283 120 73 2273 3507
Fragments arranged by size:
3507 2273 1283 1202 429 120 73
BsrDI GCAATG_nn'
Cuts at: 65 1248 3885 7382 7556 65
Size: 1183 2637 3497 174 1396
Fragments arranged by size:
3497 2637 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3472 4557 4921 7531 7718 1700
Size: 110 1662 1085 364 2610 187 2869
Fragments arranged by size:
2869 2610 1662 1085 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8887
BssHII G'CGCG_C
Cuts at: 1925 2894 1925
Size: 969 7918
BssSI C'ACGA_G
Cuts at: 6947 7959 6947
Size: 1012 7875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3912 794
Size: 3118 5769
BstBI TT'CG_AA
Cuts at: 2007 7691 2007
Size: 5684 3203
BstEII G'GTnAC_C
Cuts at: 1184 5150 1184
Size: 3966 4921
BstXI CCAn_nnnn'nTGG
Cuts at: 3746 5863 3746
Size: 2117 6770
BstYI r'GATC_y
Cuts at: 1972 2209 2301 3691 4405 4503 5278 5388 6217
Size: 237 92 1390 714 98 775 110 829
Cuts at: 6217 6584 6969 6986 8438 8524 8536 8882 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1390 829 775 714 385 367
346 237 110 98 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2028 3594 2028
Size: 1566 7321
BtgI C'CryG_G
Cuts at: 513 1065 2148 5724 513
Size: 552 1083 3576 3676
Fragments arranged by size:
3676 3576 1083 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8148
Fragments arranged by size:
8148 683 56
BtsI GCAGTG_nn'
Cuts at: 4382 6403 7197 7217 4382
Size: 2021 794 20 6052
Fragments arranged by size:
6052 2021 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5394 5429 529
Size: 35 4830 35 3987
Fragments arranged by size:
4830 3987 35 35
DraI TTT'AAA
Cuts at: 6191 6191
Size: 8887
DraIII CAC_nnn'GTG
Cuts at: 2115 2115
Size: 8887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2429 3488 5489 7894 809
Size: 507 1113 1059 2001 2405 1802
Fragments arranged by size:
2405 2001 1802 1113 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 3060 4364
Size: 54 1425 50 275 18 250 980 1304
Cuts at: 4364 4762 6071 6334 7223 8852 8
Size: 398 1309 263 889 1629 43
Fragments arranged by size:
1629 1425 1309 1304 980 889 398 275
263 250 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6334 1537
Size: 275 4522 4090
Fragments arranged by size:
4522 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2080 3038 3207 3212 3254 3795 3799 3810
Size: 727 958 169 5 42 541 4 11
Cuts at: 3810 5618 6817 1353
Size: 1808 1199 3423
Fragments arranged by size:
3423 1808 1199 958 727 541 169 42
11 5 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 2640 2801 3006 4538 7474
Size: 1064 138 678 373 161 205 1532 2936
Cuts at: 7474 7848 7994 387
Size: 374 146 1280
Fragments arranged by size:
2936 1532 1280 1064 678 374 373 205
161 146 138
EcoICRI GAG'CTC
Cuts at: 2175 2506 2616 2944 5425 2175
Size: 331 110 328 2481 5637
Fragments arranged by size:
5637 2481 331 328 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2230 2351 2531 2885 3098 3179
Size: 393 582 16 121 180 354 213 81
Cuts at: 3179 3224 3540 3623 3672 3780 3848 4122 4182
Size: 45 316 83 49 108 68 274 60
Cuts at: 4182 4415 4854 4898 5064 5318 5766 5839 6950
Size: 233 439 44 166 254 448 73 1111
Cuts at: 6950 7547 8334 8602 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 582 448 439 393
354 316 274 268 254 233 213 180
166 121 108 83 81 73 68 60
49 45 44 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2374 3194 4200 4842 5670 5913 2374
Size: 820 1006 642 828 243 5348
Fragments arranged by size:
5348 1006 828 820 642 243
EcoO109I rG'GnC_Cy
Cuts at: 2267 2380 2411 2431 2791 3435 3481 3598 3639
Size: 113 31 20 360 644 46 117 41
Cuts at: 3639 3845 4112 4703 4901 5218 5287 5695 5834
Size: 206 267 591 198 317 69 408 139
Cuts at: 5834 5906 5919 5920 6147 2267
Size: 72 13 1 227 5007
Fragments arranged by size:
5007 644 591 408 360 317 267 227
206 198 139 117 113 72 69 46
41 31 20 13 1
EcoRI G'AATT_C
Cuts at: 6196 6574 6196
Size: 378 8509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3317 3317
Size: 8887
FspI TGC'GCA
Cuts at: 7393 7393
Size: 8887
HaeII r_GCGC'y
Cuts at: 1678 2538 2680 3247 4332 8034 1678
Size: 860 142 567 1085 3702 2531
Fragments arranged by size:
3702 2531 1085 860 567 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 2928 2960 3115
Size: 32 135 32 462 32 862 32 155
Cuts at: 3115 3147 3177 3209 3220 3252 5782 5814 7528
Size: 32 30 32 11 32 2530 32 1714
Cuts at: 7528 7560 7602 7634 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2530 1714 862 462 155 135 42
32 32 32 32 32 32 32 32
32 32 30 11
HincII GTy'rAC
Cuts at: 669 1657 1938 5240 6040 6231 6477 669
Size: 988 281 3302 800 191 246 3079
Fragments arranged by size:
3302 3079 988 800 281 246 191
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8887
HpaI GTT'AAC
Cuts at: 6040 6477 6040
Size: 437 8450
KpnI G_GTAC'C
Cuts at: 5349 6212 5349
Size: 863 8024
MfeI C'AATT_G
Cuts at: 6486 6486
Size: 8887
MluI A'CGCG_T
Cuts at: 8763 8763
Size: 8887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2949 5595 5754 8001 8185 1305
Size: 1644 2646 159 2247 184 2007
Fragments arranged by size:
2646 2247 2007 1644 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 4764 10
Size: 54 1425 343 1230 1702 4133
Fragments arranged by size:
4133 1702 1425 1230 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4675 5132 6047 6845 7204 7363 518
Size: 648 3509 457 915 798 359 159 2042
Fragments arranged by size:
3509 2042 915 798 648 457 359 159
MspA1I CmG'CkG
Cuts at: 1606 2846 2914 3893 3963 4130 4357 4422 4519
Size: 1240 68 979 70 167 227 65 97
Cuts at: 4519 4823 5549 5651 5846 6303 6980 8128 8373
Size: 304 726 102 195 457 677 1148 245
Cuts at: 8373 1606
Size: 2120
Fragments arranged by size:
2120 1240 1148 979 726 677 457 304
245 227 195 167 102 97 70 68
65
NaeI GCC'GGC
Cuts at: 4559 4923 4559
Size: 364 8523
NcoI C'CATG_G
Cuts at: 513 1065 2148 5724 513
Size: 552 1083 3576 3676
Fragments arranged by size:
3676 3576 1083 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8887
NgoMIV G'CCGG_C
Cuts at: 4557 4921 4557
Size: 364 8523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8887
NotI GC'GGCC_GC
Cuts at: 6334 6334
Size: 8887
NruI TCG'CGA
Cuts at: 2811 2811
Size: 8887
NspI r_CATG'y
Cuts at: 1132 1229 2891 4680 4778 4862 6181 6245 7790
Size: 97 1662 1789 98 84 1319 64 1545
Cuts at: 7790 1132
Size: 2229
Fragments arranged by size:
2229 1789 1662 1545 1319 98 97 84
64
PasI CC'CwG_GG
Cuts at: 1348 2219 3404 4699 5284 6151 1348
Size: 871 1185 1295 585 867 4084
Fragments arranged by size:
4084 1295 1185 871 867 585
PciI A'CATG_T
Cuts at: 4676 4774 6177 7786 4676
Size: 98 1403 1609 5777
Fragments arranged by size:
5777 1609 1403 98
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2219 2801 3431 3865 5890 1108
Size: 1111 582 630 434 2025 4105
Fragments arranged by size:
4105 2025 1111 630 582 434
PfoI T'CCnGG_A
Cuts at: 2508 2631 2901 3660 3864 4392 2508
Size: 123 270 759 204 528 7003
Fragments arranged by size:
7003 759 528 270 204 123
PmeI GTTT'AAAC
Cuts at: 6191 6191
Size: 8887
PmlI CAC'GTG
Cuts at: 4645 6049 4645
Size: 1404 7483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 6956 6988 8469 8501 2963
Size: 32 3961 32 1481 32 3349
Fragments arranged by size:
3961 3349 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2431 3481 3598 3639 4703 2380
Size: 51 1050 117 41 1064 6564
Fragments arranged by size:
6564 1064 1050 117 51 41
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8887
PsiI TTA'TAA
Cuts at: 6457 6457
Size: 8887
PspOMI G'GGCC_C
Cuts at: 2222 2410 2791 3434 5218 5287 5833 5919 6147
Size: 188 381 643 1784 69 546 86 228
Cuts at: 6147 2222
Size: 4962
Fragments arranged by size:
4962 1784 643 546 381 228 188 86
69
PstI C_TGCA'G
Cuts at: 830 2140 2673 2712 2766 4122 5237 6239 830
Size: 1310 533 39 54 1356 1115 1002 3478
Fragments arranged by size:
3478 1356 1310 1115 1002 533 54 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8887
PvuII CAG'CTG
Cuts at: 3893 4130 4357 4823 5651 5846 3893
Size: 237 227 466 828 195 6934
Fragments arranged by size:
6934 828 466 237 227 195
SacI G_AGCT'C
Cuts at: 2177 2508 2618 2946 5427 2177
Size: 331 110 328 2481 5637
Fragments arranged by size:
5637 2481 331 328 110
SalI G'TCGA_C
Cuts at: 1936 6229 1936
Size: 4293 4594
SapI GCTCTTCn'nnn_
Cuts at: 3038 5618 3038
Size: 2580 6307
SbfI CC_TGCA'GG
Cuts at: 6239 6239
Size: 8887
ScaI AGT'ACT
Cuts at: 7135 8847 7135
Size: 1712 7175
SfcI C'TryA_G
Cuts at: 826 1045 2136 2669 2708 2762 4118 5233 6235
Size: 219 1091 533 39 54 1356 1115 1002
Cuts at: 6235 7370 8051 8242 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1356 1135 1115 1091 1002 681 533
219 191 54 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8887
SmaI CCC'GGG
Cuts at: 1872 2345 5936 6214 1872
Size: 473 3591 278 4545
Fragments arranged by size:
4545 3591 473 278
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2323 2744 3381 6991 7892 8154
Size: 197 926 380 421 637 3610 901 262
Cuts at: 8154 820
Size: 1553
Fragments arranged by size:
3610 1553 926 901 637 421 380 262
197
SnaBI TAC'GTA
Cuts at: 493 6184 6678 493
Size: 5691 494 2702
Fragments arranged by size:
5691 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8887
SphI G_CATG'C
Cuts at: 1132 2891 6245 1132
Size: 1759 3354 3774
Fragments arranged by size:
3774 3354 1759
SspI AAT'ATT
Cuts at: 5 52 6811 5
Size: 47 6759 2081
Fragments arranged by size:
6759 2081 47
StuI AGG'CCT
Cuts at: 1517 3684 1517
Size: 2167 6720
StyI C'CwwG_G
Cuts at: 513 1065 2038 2148 3282 4108 5091 5724 6078
Size: 552 973 110 1134 826 983 633 354
Cuts at: 6078 6326 513
Size: 248 3074
Fragments arranged by size:
3074 1134 983 973 826 633 552 354
248 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 3722 4286 4400 4968 5527 5719
Size: 1255 1332 810 564 114 568 559 192
Cuts at: 5719 6914 6931 7084 7269 7714 325
Size: 1195 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 1195 810 568 564 559
445 192 185 153 114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3081 7133
Size: 274 80 33 51 1443 618 486 4052
Cuts at: 7133 8845 96
Size: 1712 138
Fragments arranged by size:
4052 1712 1443 618 486 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3846 3878 4374 4406 5425 5457 8847
Size: 32 560 32 496 32 1019 32 3390
Cuts at: 8847 8879 3254
Size: 32 3262
Fragments arranged by size:
3390 3262 1019 560 496 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4462 5228 1316
Size: 3146 766 4975
Fragments arranged by size:
4975 3146 766
XbaI T'CTAG_A
Cuts at: 5683 6223 5683
Size: 540 8347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2661 2661
Size: 8887
XhoI C'TCGA_G
Cuts at: 1943 3381 1943
Size: 1438 7449
XmaI C'CCGG_G
Cuts at: 1870 2343 5934 6212 1870
Size: 473 3591 278 4545
Fragments arranged by size:
4545 3591 473 278
XmnI GAAnn'nnTTC
Cuts at: 7016 7016
Size: 8887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6695 276
Size: 53 83 186 936 5161 2468
Fragments arranged by size:
5161 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NarI NsiI PacI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI