Restriction map for
pFN21ASDF0002
(Circular) (Six-base) MAPSORT of: pFN21ASDF0002.seq Check: 2666 from: 1 to: 9205
pFN21ASDF0002 9205 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4320 4320
Size: 9205
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7015 278
Size: 53 83 186 936 5479 2468
Fragments arranged by size:
5479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3543 4242 6548 1937
Size: 1606 699 2306 4594
Fragments arranged by size:
4594 2306 1606 699
Acc65I G'GTAC_C
Cuts at: 3322 6526 3322
Size: 3204 6001
AclI AA'CG_TT
Cuts at: 1563 7332 7705 1563
Size: 5769 373 3063
Fragments arranged by size:
5769 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 2915 3065 4781 6110 7268 8652 8920
Size: 1053 230 150 1716 1329 1158 1384 268
Cuts at: 8920 1632
Size: 1917
Fragments arranged by size:
1917 1716 1384 1329 1158 1053 268 230
150
AfeI AGC'GCT
Cuts at: 5515 5515
Size: 9205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9008
AflIII A'CryG_T
Cuts at: 1203 4635 8104 9081 1203
Size: 3432 3469 977 1327
Fragments arranged by size:
3469 3432 1327 977
AgeI A'CCGG_T
Cuts at: 3562 8036 3562
Size: 4474 4731
AhdI GACnn_n'nnGTC
Cuts at: 3512 7934 3512
Size: 4422 4783
AleI CACnn'nnGTG
Cuts at: 1166 2839 3629 6105 1166
Size: 1673 790 2476 4266
Fragments arranged by size:
4266 2476 1673 790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2519 2577 3405 1184
Size: 1335 58 828 6984
Fragments arranged by size:
6984 1335 828 58
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4029 4061 4029
Size: 32 9173
AlwNI CAG_nnn'CTG
Cuts at: 2163 2597 3463 5473 5722 6088 6451 6454 6948
Size: 434 866 2010 249 366 363 3 494
Cuts at: 6948 8520 2163
Size: 1572 2848
Fragments arranged by size:
2848 2010 1572 866 494 434 366 363
249 3
ApaI G_GGCC'C
Cuts at: 2526 2931 3138 2526
Size: 405 207 8593
Fragments arranged by size:
8593 405 207
ApaLI G'TGCA_C
Cuts at: 7262 8418 7262
Size: 1156 8049
ApoI r'AATT_y
Cuts at: 1493 4954 6514 6740 6892 9001 1493
Size: 3461 1560 226 152 2109 1697
Fragments arranged by size:
3461 2109 1697 1560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7759 160
Size: 872 6727 1606
Fragments arranged by size:
6727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9205
AvaI C'yCGr_G
Cuts at: 1870 1943 2179 2412 2639 3471 3492 5617 6530
Size: 73 236 233 227 832 21 2125 913
Cuts at: 6530 1870
Size: 4545
Fragments arranged by size:
4545 2125 913 832 236 233 227 73
21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4132 4165 4132
Size: 33 9172
BamHI G'GATC_C
Cuts at: 3732 4044 5091 6535 6902 3732
Size: 312 1047 1444 367 6035
Fragments arranged by size:
6035 1444 1047 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2220 2710 3322 3609
Size: 325 217 627 387 46 490 612 287
Cuts at: 3609 4422 4619 4997 5053 5301 5439 5732 6526
Size: 813 197 378 56 248 138 293 794
Cuts at: 6526 7981 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 813 794 627 612 490 387
378 325 293 287 248 217 197 138
56 46
BanII G_rGCy'C
Cuts at: 1391 2526 2623 2631 2745 2931 3138 3472 3493
Size: 1135 97 8 114 186 207 334 21
Cuts at: 3493 3944 3972 4260 4821 5190 5207 5919 6012
Size: 451 28 288 561 369 17 712 93
Cuts at: 6012 6208 6524 1391
Size: 196 316 4072
Fragments arranged by size:
4072 1135 712 561 451 369 334 316
288 207 196 186 114 97 93 28
21 17 8
BbeI G_GCGC'C
Cuts at: 2224 4426 4623 5305 2224
Size: 2202 197 682 6124
Fragments arranged by size:
6124 2202 682 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2248 2800 4091 5874 6272 928
Size: 971 349 552 1291 1783 398 3861
Fragments arranged by size:
3861 1783 1291 971 552 398 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7396 7430 1903
Size: 34 5459 34 3678
Fragments arranged by size:
5459 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 7097 8307 4160
Size: 2937 1210 5058
Fragments arranged by size:
5058 2937 1210
BclI T'GATC_A
Cuts at: 1548 3693 1548
Size: 2145 7060
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 2708 2729
Size: 107 122 71 631 605 821 215 21
Cuts at: 2729 3020 3488 5171 5384 5868 7816 136
Size: 291 468 1683 213 484 1948 1525
Fragments arranged by size:
1948 1683 1525 821 631 605 484 468
291 215 213 122 107 71 21
BglII A'GATC_T
Cuts at: 9200 9200
Size: 9205
BlpI GC'TnA_GC
Cuts at: 2123 4340 6622 2123
Size: 2217 2282 4706
Fragments arranged by size:
4706 2282 2217
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2526 2836 2931 3138 3612 4219 5735
Size: 387 349 310 95 207 474 607 1516
Cuts at: 5735 7266 8422 1790
Size: 1531 1156 2573
Fragments arranged by size:
2573 1531 1516 1156 607 474 387 349
310 207 95
BmgBI CAC'GTC
Cuts at: 1318 5773 1318
Size: 4455 4750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2591 3162 3473
Size: 416 53 83 399 24 1174 571 311
Cuts at: 3473 3842 4519 4862 5913 5980 6936 7894 442
Size: 369 677 343 1051 67 956 958 1753
Fragments arranged by size:
1753 1174 1051 958 956 677 571 416
399 369 343 311 83 67 53 24
BmtI G_CTAG'C
Cuts at: 1056 3014 1056
Size: 1958 7247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4528 4792 6186 6421 4528
Size: 264 1394 235 7312
Fragments arranged by size:
7312 1394 264 235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2439 3728 3884 3984 4182 4406 4583 5859
Size: 1200 1289 156 100 198 224 177 1276
Cuts at: 5859 5984 6266 7865 1239
Size: 125 282 1599 2579
Fragments arranged by size:
2579 1599 1289 1276 1200 282 224 198
177 156 125 100
Bpu10I CC'TnA_GC
Cuts at: 2622 4199 4678 2622
Size: 1577 479 7149
Fragments arranged by size:
7149 1577 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5444 7330 8195 8493 5444
Size: 1886 865 298 6156
Fragments arranged by size:
6156 1886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3305 3707 3860 4158 7868 882
Size: 617 1806 402 153 298 3710 2219
Fragments arranged by size:
3710 2219 1806 617 402 298 153
BsaAI yAC'GTr
Cuts at: 493 1204 4636 5263 6502 6996 493
Size: 711 3432 627 1239 494 2702
Fragments arranged by size:
3432 2702 1239 711 627 494
BsaBI GATnn'nnATC
Cuts at: 9199 9199
Size: 9205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2221 4423 4620 5302
Size: 53 83 186 936 688 2202 197 682
Cuts at: 5302 7012 7394 8029 275
Size: 1710 382 635 1451
Fragments arranged by size:
2202 1710 1451 936 688 682 635 382
197 186 83 53
BsaWI w'CCGG_w
Cuts at: 3562 3729 7638 8036 8310 8457 8868 3562
Size: 167 3909 398 274 147 411 3899
Fragments arranged by size:
3909 3899 411 398 274 167 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3459 3489 5090 5120 5267
Size: 30 165 30 2056 30 1601 30 147
Cuts at: 5267 5297 5308 5338 1178
Size: 30 11 30 5045
Fragments arranged by size:
5045 2056 1601 165 147 30 30 30
30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2197 4256 4580 4583 4829 4894 5098
Size: 949 62 2059 324 3 246 65 204
Cuts at: 5098 5275 5417 5420 6200 1186
Size: 177 142 3 780 4191
Fragments arranged by size:
4191 2059 949 780 324 246 204 177
142 65 62 3 3
BseYI C'CCAG_C
Cuts at: 2040 2112 3028 3130 3137 3206 3499 4804 5182
Size: 72 916 102 7 69 293 1305 378
Cuts at: 5182 5200 5728 5973 6145 8408 8904 2040
Size: 18 528 245 172 2263 496 2341
Fragments arranged by size:
2341 2263 1305 916 528 496 378 293
245 172 102 72 69 18 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 4301 4348 4948 5285 5558 5852
Size: 573 811 1754 47 600 337 273 294
Cuts at: 5852 1163
Size: 4516
Fragments arranged by size:
4516 1754 811 600 573 337 294 273
47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6655 7416 8444 1540
Size: 275 186 4654 761 1028 2301
Fragments arranged by size:
4654 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2157 2745 3944 3972 4260 4580 4986 5373
Size: 33 588 1199 28 288 320 406 387
Cuts at: 5373 5901 7266 7351 8422 2124
Size: 528 1365 85 1071 2907
Fragments arranged by size:
2907 1365 1199 1071 588 528 406 387
320 288 85 33 28
BsmI GAATG_Cn'
Cuts at: 3426 3663 5809 6716 6809 3426
Size: 237 2146 907 93 5822
Fragments arranged by size:
5822 2146 907 237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2157 2177 2526 2623 2631 2745
Size: 399 334 33 20 349 97 8 114
Cuts at: 2745 2836 2931 3138 3472 3493 3612 3944 3972
Size: 91 95 207 334 21 119 332 28
Cuts at: 3972 4219 4260 4580 4821 4986 5190 5207 5373
Size: 247 41 320 241 165 204 17 166
Cuts at: 5373 5735 5901 5919 6012 6208 6524 7266 7351
Size: 362 166 18 93 196 316 742 85
Cuts at: 7351 8422 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 349 334 334
332 320 316 247 241 207 204 196
166 166 165 119 114 97 95 93
91 85 41 33 28 21 20 18
17 8
BspEI T'CCGG_A
Cuts at: 8868 8868
Size: 9205
BspHI T'CATG_A
Cuts at: 7094 8824 7094
Size: 1730 7475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3265 3270 4320 5421 5703 6560 844
Size: 2421 5 1050 1101 282 857 3489
Fragments arranged by size:
3489 2421 1101 1050 857 282 5
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 5671 7092 1394
Size: 429 248 3600 1421 3507
Fragments arranged by size:
3600 3507 1421 429 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 2482 4941 6327 7700 7874 65
Size: 1183 1234 2459 1386 1373 174 1396
Fragments arranged by size:
2459 1396 1386 1373 1234 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3014 3562 5735 5787 7849 8036 1700
Size: 110 1204 548 2173 52 2062 187 2869
Fragments arranged by size:
2869 2173 2062 1204 548 187 110 52
BsrGI T'GTAC_A
Cuts at: 96 4104 96
Size: 4008 5197
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9205
BssSI C'ACGA_G
Cuts at: 2607 2802 4233 7265 8277 2607
Size: 195 1431 3032 1012 3535
Fragments arranged by size:
3535 3032 1431 1012 195
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6454 794
Size: 5660 3545
BstBI TT'CG_AA
Cuts at: 2007 8009 2007
Size: 6002 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9205
BstXI CCAn_nnnn'nTGG
Cuts at: 5962 5962
Size: 9205
BstYI r'GATC_y
Cuts at: 1972 3732 3787 4044 4749 5091 5981 6225 6535
Size: 1760 55 257 705 342 890 244 310
Cuts at: 6535 6902 7287 7304 8756 8842 8854 9200 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1760 1452 890 705 385 367 346
342 310 257 244 86 55 17 12
BstZ17I GTA'TAC
Cuts at: 3544 3544
Size: 9205
Bsu36I CC'TnA_GG
Cuts at: 2655 2754 2655
Size: 99 9106
BtgI C'CryG_G
Cuts at: 513 1065 2022 2526 2725 2986 4623 4659 5082
Size: 552 957 504 199 261 1637 36 423
Cuts at: 5082 6123 513
Size: 1041 3595
Fragments arranged by size:
3595 1637 1041 957 552 504 423 261
199 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3943 5009 5512 487
Size: 683 56 2717 1066 503 4180
Fragments arranged by size:
4180 2717 1066 683 503 56
BtsI GCAGTG_nn'
Cuts at: 2101 3311 3618 4002 4877 4986 5474 5511 5720
Size: 1210 307 384 875 109 488 37 209
Cuts at: 5720 6101 6721 7515 7535 2101
Size: 381 620 794 20 3771
Fragments arranged by size:
3771 1210 875 794 620 488 384 381
307 209 109 37 20
ClaI AT'CG_AT
Cuts at: 1554 6237 1554
Size: 4683 4522
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9170
DraI TTT'AAA
Cuts at: 6509 6509
Size: 9205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8212 809
Size: 507 6896 1802
Fragments arranged by size:
6896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2404 3246
Size: 54 1425 50 275 18 236 338 842
Cuts at: 3246 3357 4026 4281 4302 5163 5193 5343 5789
Size: 111 669 255 21 861 30 150 446
Cuts at: 5789 6198 6411 6652 7541 9170 8
Size: 409 213 241 889 1629 43
Fragments arranged by size:
1629 1425 889 861 842 669 446 409
338 275 255 241 236 213 150 111
54 50 43 30 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 6652 1537
Size: 275 4840 4090
Fragments arranged by size:
4840 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2958 3932 4694 4826 5673 5906 7135 1353
Size: 1605 974 762 132 847 233 1229 3423
Fragments arranged by size:
3423 1605 1229 974 847 762 233 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7792 8166 8312 387
Size: 1064 138 6203 374 146 1280
Fragments arranged by size:
6203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 3942 3970 4258 2743
Size: 1199 28 288 7690
Fragments arranged by size:
7690 1199 288 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 2685 2915 3065 3728 3884 3984
Size: 393 807 246 230 150 663 156 100
Cuts at: 3984 4182 4406 4583 4781 5859 5984 6110 6266
Size: 198 224 177 198 1078 125 126 156
Cuts at: 6266 7268 7865 8652 8920 1239
Size: 1002 597 787 268 1524
Fragments arranged by size:
1524 1078 1002 807 787 663 597 393
268 246 230 224 198 198 177 156
156 150 126 125 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2639 2963 4595 5699 6062 6107 2639
Size: 324 1632 1104 363 45 5737
Fragments arranged by size:
5737 1632 1104 363 324 45
EcoO109I rG'GnC_Cy
Cuts at: 2309 2417 2522 2730 2847 2859 2927 2928 3134
Size: 108 105 208 117 12 68 1 206
Cuts at: 3134 3160 3229 3844 5280 5551 6055 6072 6085
Size: 26 69 615 1436 271 504 17 13
Cuts at: 6085 6494 2309
Size: 409 5020
Fragments arranged by size:
5020 1436 615 504 409 271 208 206
117 108 105 69 68 26 17 13
12 1
EcoRI G'AATT_C
Cuts at: 6514 6892 6514
Size: 378 8827
FspI TGC'GCA
Cuts at: 6422 7711 6422
Size: 1289 7916
HaeII r_GCGC'y
Cuts at: 1678 2130 2224 4426 4623 5272 5305 5517 8352
Size: 452 94 2202 197 649 33 212 2835
Cuts at: 8352 1678
Size: 2531
Fragments arranged by size:
2835 2531 2202 649 452 212 197 94
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 3498 3530 3685
Size: 32 135 32 636 32 1258 32 155
Cuts at: 3685 3717 4056 4088 7846 7878 7920 7952 1373
Size: 32 339 32 3758 32 42 32 2626
Fragments arranged by size:
3758 2626 1258 636 339 155 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 6549 6795 669
Size: 988 281 1393 3218 246 3079
Fragments arranged by size:
3218 3079 1393 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3401 4644 748
Size: 2653 1243 5309
Fragments arranged by size:
5309 2653 1243
HpaI GTT'AAC
Cuts at: 6795 6795
Size: 9205
KasI G'GCGC_C
Cuts at: 2220 4422 4619 5301 2220
Size: 2202 197 682 6124
Fragments arranged by size:
6124 2202 682 197
KpnI G_GTAC'C
Cuts at: 3326 6530 3326
Size: 3204 6001
MfeI C'AATT_G
Cuts at: 6804 6804
Size: 9205
MluI A'CGCG_T
Cuts at: 9081 9081
Size: 9205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2251 3832 8319 8503 1305
Size: 946 1581 4487 184 2007
Fragments arranged by size:
4487 2007 1581 946 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 3248 3359 4028 4304
Size: 54 1425 343 574 842 111 669 276
Cuts at: 4304 5165 6200 10
Size: 861 1035 3015
Fragments arranged by size:
3015 1425 1035 861 842 669 574 343
276 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2584 2839 3387 3629 4868 5657 6105
Size: 648 1418 255 548 242 1239 789 448
Cuts at: 6105 7163 7522 7681 518
Size: 1058 359 159 2042
Fragments arranged by size:
2042 1418 1239 1058 789 648 548 448
359 255 242 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2077 2116 2263 2348 2557 3130 4102
Size: 438 33 39 147 85 209 573 972
Cuts at: 4102 4286 4759 4834 5012 5728 5962 6187 6277
Size: 184 473 75 178 716 234 225 90
Cuts at: 6277 6451 6621 7298 8446 8691 1606
Size: 174 170 677 1148 245 2120
Fragments arranged by size:
2120 1148 972 716 677 573 473 438
245 234 225 209 184 178 174 170
147 90 85 75 39 33
NarI GG'CG_CC
Cuts at: 2221 4423 4620 5302 2221
Size: 2202 197 682 6124
Fragments arranged by size:
6124 2202 682 197
NcoI C'CATG_G
Cuts at: 513 1065 2022 2526 6123 513
Size: 552 957 504 3597 3595
Fragments arranged by size:
3597 3595 957 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9205
NheI G'CTAG_C
Cuts at: 1052 3010 1052
Size: 1958 7247
NotI GC'GGCC_GC
Cuts at: 6652 6652
Size: 9205
NspI r_CATG'y
Cuts at: 1132 1229 3992 4339 6563 8108 1132
Size: 97 2763 347 2224 1545 2229
Fragments arranged by size:
2763 2229 2224 1545 347 97
PasI CC'CwG_GG
Cuts at: 1348 2519 2616 5564 5710 5749 6052 1348
Size: 1171 97 2948 146 39 303 4501
Fragments arranged by size:
4501 2948 1171 303 146 97 39
PciI A'CATG_T
Cuts at: 8104 8104
Size: 9205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 4485 4672 5773 1108
Size: 1263 2114 187 1101 4540
Fragments arranged by size:
4540 2114 1263 1101 187
PfoI T'CCnGG_A
Cuts at: 2212 2958 3705 5766 2212
Size: 746 747 2061 5651
Fragments arranged by size:
5651 2061 747 746
PmeI GTTT'AAAC
Cuts at: 6509 6509
Size: 9205
PmlI CAC'GTG
Cuts at: 4636 5263 4636
Size: 627 8578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7274 7306 8787 8819 7274
Size: 32 1481 32 7660
Fragments arranged by size:
7660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2309 2417 3844 6494 2309
Size: 108 1427 2650 5020
Fragments arranged by size:
5020 2650 1427 108
PshAI GACnn'nnGTC
Cuts at: 1613 4504 1613
Size: 2891 6314
PsiI TTA'TAA
Cuts at: 6775 6775
Size: 9205
PspOMI G'GGCC_C
Cuts at: 2522 2927 3134 2522
Size: 405 207 8593
Fragments arranged by size:
8593 405 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 9173
PstI C_TGCA'G
Cuts at: 830 2210 2556 2787 3045 3799 4599 5727 6459
Size: 1380 346 231 258 754 800 1128 732
Cuts at: 6459 6557 830
Size: 98 3478
Fragments arranged by size:
3478 1380 1128 800 754 732 346 258
231 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9205
PvuII CAG'CTG
Cuts at: 2044 2116 2348 2557 3130 4102 4759 4834 5012
Size: 72 232 209 573 972 657 75 178
Cuts at: 5012 5728 5962 6187 6277 6451 2044
Size: 716 234 225 90 174 4798
Fragments arranged by size:
4798 972 716 657 573 234 232 225
209 178 174 90 75 72
SacI G_AGCT'C
Cuts at: 2745 3944 3972 4260 2745
Size: 1199 28 288 7690
Fragments arranged by size:
7690 1199 288 28
SalI G'TCGA_C
Cuts at: 1936 6547 1936
Size: 4611 4594
SanDI GG'GwC_CC
Cuts at: 2417 3844 2417
Size: 1427 7778
SapI GCTCTTCn'nnn_
Cuts at: 3932 4826 5906 3932
Size: 894 1080 7231
Fragments arranged by size:
7231 1080 894
SbfI CC_TGCA'GG
Cuts at: 6557 6557
Size: 9205
ScaI AGT'ACT
Cuts at: 7453 9165 7453
Size: 1712 7493
SfcI C'TryA_G
Cuts at: 826 1045 2206 2552 2783 3041 3377 3778 3795
Size: 219 1161 346 231 258 336 401 17
Cuts at: 3795 3807 4595 5275 5392 5486 5723 6455 6553
Size: 12 788 680 117 94 237 732 98
Cuts at: 6553 7688 8369 8560 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1161 1135 788 732 681 680 401
346 336 258 237 231 219 191 117
98 94 17 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2729 5384 2729
Size: 2655 6550
SfoI GGC'GCC
Cuts at: 2222 4424 4621 5303 2222
Size: 2202 197 682 6124
Fragments arranged by size:
6124 2202 682 197
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9205
SmaI CCC'GGG
Cuts at: 1872 2414 6532 1872
Size: 542 4118 4545
Fragments arranged by size:
4545 4118 542
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3492 5423 7309 8210 8472 820
Size: 197 926 1549 1931 1886 901 262 1553
Fragments arranged by size:
1931 1886 1553 1549 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6502 6996 493
Size: 6009 494 2702
Fragments arranged by size:
6009 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9205
SphI G_CATG'C
Cuts at: 1132 4339 6563 1132
Size: 3207 2224 3774
Fragments arranged by size:
3774 3207 2224
SspI AAT'ATT
Cuts at: 5 52 7129 5
Size: 47 7077 2081
Fragments arranged by size:
7077 2081 47
StuI AGG'CCT
Cuts at: 1517 3482 3773 5178 1517
Size: 1965 291 1405 5544
Fragments arranged by size:
5544 1965 1405 291
StyI C'CwwG_G
Cuts at: 513 1065 2022 2421 2427 2526 3627 3686 3723
Size: 552 957 399 6 99 1101 59 37
Cuts at: 3723 4011 5352 6123 6644 513
Size: 288 1341 771 521 3074
Fragments arranged by size:
3074 1341 1101 957 771 552 521 399
288 99 59 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 2371 3020 3284 3679 4046 4382
Size: 1255 546 245 649 264 395 367 336
Cuts at: 4382 5095 6116 6137 7232 7249 7402 7587 8032
Size: 713 1021 21 1095 17 153 185 445
Cuts at: 8032 325
Size: 1498
Fragments arranged by size:
1498 1255 1095 1021 713 649 546 445
395 367 336 264 245 185 153 21
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3111 4104 4531
Size: 274 80 33 51 1443 1134 993 427
Cuts at: 4531 5253 5682 7451 9163 96
Size: 722 429 1769 1712 138
Fragments arranged by size:
1769 1712 1443 1134 993 722 429 427
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5308 5340 9165 9197 5308
Size: 32 3825 32 5316
Fragments arranged by size:
5316 3825 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9205
XbaI T'CTAG_A
Cuts at: 6541 6541
Size: 9205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2613 3352 3721 3980 6130 6253 2613
Size: 739 369 259 2150 123 5565
Fragments arranged by size:
5565 2150 739 369 259 123
XhoI C'TCGA_G
Cuts at: 1943 3492 1943
Size: 1549 7656
XmaI C'CCGG_G
Cuts at: 1870 2412 6530 1870
Size: 542 4118 4545
Fragments arranged by size:
4545 4118 542
XmnI GAAnn'nnTTC
Cuts at: 2031 7334 2031
Size: 5303 3902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7013 276
Size: 53 83 186 936 5479 2468
Fragments arranged by size:
5479 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbvCI BfrBI BsiWI BsmBI DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI RsrII SacII SexAI SrfI SwaI