Restriction map for
pFN21ASDB3800
(Circular) (Six-base) MAPSORT of: pFN21ASDB3800.seq Check: 9845 from: 1 to: 8698
pFN21ASDB3800 8698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6508 278
Size: 53 83 186 936 4972 2468
Fragments arranged by size:
4972 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5069 5991 6041 1937
Size: 3132 922 50 4594
Fragments arranged by size:
4594 3132 922 50
Acc65I G'GTAC_C
Cuts at: 3805 4135 6019 3805
Size: 330 1884 6484
Fragments arranged by size:
6484 1884 330
AclI AA'CG_TT
Cuts at: 1563 5538 6825 7198 1563
Size: 3975 1287 373 3063
Fragments arranged by size:
3975 3063 1287 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2724 4437 4759 5038 5798 6761 8145 8413
Size: 1092 1713 322 279 760 963 1384 268
Cuts at: 8413 1632
Size: 1917
Fragments arranged by size:
1917 1713 1384 1092 963 760 322 279
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8501
AflIII A'CryG_T
Cuts at: 1203 5809 7597 8574 1203
Size: 4606 1788 977 1327
Fragments arranged by size:
4606 1788 1327 977
AgeI A'CCGG_T
Cuts at: 7529 7529
Size: 8698
AhdI GACnn_n'nnGTC
Cuts at: 7427 7427
Size: 8698
AleI CACnn'nnGTG
Cuts at: 1166 2655 1166
Size: 1489 7209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5408 5440 5408
Size: 32 8666
AlwNI CAG_nnn'CTG
Cuts at: 2436 4843 5440 6441 8013 2436
Size: 2407 597 1001 1572 3121
Fragments arranged by size:
3121 2407 1572 1001 597
ApaI G_GGCC'C
Cuts at: 5948 5948
Size: 8698
ApaLI G'TGCA_C
Cuts at: 6755 7911 6755
Size: 1156 7542
ApoI r'AATT_y
Cuts at: 1493 2338 2638 2870 3486 3753 4555 5130 6007
Size: 845 300 232 616 267 802 575 877
Cuts at: 6007 6233 6385 8494 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 877 845 802 616 575 300
267 232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7252 160
Size: 872 6220 1606
Fragments arranged by size:
6220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
AvaI C'yCGr_G
Cuts at: 1870 1943 6023 1870
Size: 73 4080 4545
Fragments arranged by size:
4545 4080 73
AvrII C'CTAG_G
Cuts at: 2615 2615
Size: 8698
BamHI G'GATC_C
Cuts at: 2356 6028 6395 2356
Size: 3672 367 4659
Fragments arranged by size:
4659 3672 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3805 4135 5201 5312 5905
Size: 325 217 627 2018 330 1066 111 593
Cuts at: 5905 6019 7474 618
Size: 114 1455 1842
Fragments arranged by size:
2018 1842 1455 1066 627 593 330 325
217 114 111
BanII G_rGCy'C
Cuts at: 1391 4037 4108 4115 5948 6017 1391
Size: 2646 71 7 1833 69 4072
Fragments arranged by size:
4072 2646 1833 71 69 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 928
Size: 971 159 7568
Fragments arranged by size:
7568 971 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4706 4740 5533 5567 6889 6923 1903
Size: 34 2769 34 793 34 1322 34 3678
Fragments arranged by size:
3678 2769 1322 793 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5351 6590 7800 5351
Size: 1239 1210 6249
Fragments arranged by size:
6249 1239 1210
BclI T'GATC_A
Cuts at: 1548 2086 2293 1548
Size: 538 207 7953
Fragments arranged by size:
7953 538 207
BfrBI ATG'CAT
Cuts at: 2179 4627 2179
Size: 2448 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7309 136
Size: 107 122 71 631 605 5637 1525
Fragments arranged by size:
5637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2387 2623 3681 8693 2387
Size: 236 1058 5012 2392
Fragments arranged by size:
5012 2392 1058 236
BlpI GC'TnA_GC
Cuts at: 4176 6115 4176
Size: 1939 6759
Bme1580I G_kGCm'C
Cuts at: 1790 5948 6759 7915 1790
Size: 4158 811 1156 2573
Fragments arranged by size:
4158 2573 1156 811
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4298 5810 6429
Size: 416 53 83 399 24 2881 1512 619
Cuts at: 6429 7387 442
Size: 958 1753
Fragments arranged by size:
2881 1753 1512 958 619 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 4389 2911
Size: 1478 7220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3696 3780 4182 7358 1239
Size: 2457 84 402 3176 2579
Fragments arranged by size:
3176 2579 2457 402 84
Bpu10I CC'TnA_GC
Cuts at: 4092 4092
Size: 8698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3981 4009 5449 6823 7688 7986 3981
Size: 28 1440 1374 865 298 4693
Fragments arranged by size:
4693 1440 1374 865 298 28
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2472 7361 882
Size: 617 973 4889 2219
Fragments arranged by size:
4889 2219 973 617
BsaAI yAC'GTr
Cuts at: 493 1204 5810 5995 6489 493
Size: 711 4606 185 494 2702
Fragments arranged by size:
4606 2702 711 494 185
BsaBI GATnn'nnATC
Cuts at: 3729 4889 8692 3729
Size: 1160 3803 3735
Fragments arranged by size:
3803 3735 1160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6505 6887 7522 275
Size: 53 83 186 936 4972 382 635 1451
Fragments arranged by size:
4972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2715 3808 4672 7131 7529 7803 7950 8361 2715
Size: 1093 864 2459 398 274 147 411 3052
Fragments arranged by size:
3052 2459 1093 864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4060 4090 4155 4185 1178
Size: 30 165 30 2657 30 65 30 5691
Fragments arranged by size:
5691 2657 165 65 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 1186
Size: 1519 7179
BseYI C'CCAG_C
Cuts at: 5135 7901 8397 5135
Size: 2766 496 5436
Fragments arranged by size:
5436 2766 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4736 1163
Size: 573 3000 5125
Fragments arranged by size:
5125 3000 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6148 6909 7937 1540
Size: 275 186 4147 761 1028 2301
Fragments arranged by size:
4147 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2699 4108 4115 5505 6759 6844 7915 2699
Size: 1409 7 1390 1254 85 1071 3482
Fragments arranged by size:
3482 1409 1390 1254 1071 85 7
BsmI GAATG_Cn'
Cuts at: 3168 3263 3997 4629 6209 6302 3168
Size: 95 734 632 1580 93 5564
Fragments arranged by size:
5564 1580 734 632 95 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4334 5012 4334
Size: 678 8020
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2699 4037 4108 4115 5505 5948 6017
Size: 399 909 1338 71 7 1390 443 69
Cuts at: 6017 6759 6844 7915 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1390 1338 1071 909 742 443 399
85 71 69 7
BspEI T'CCGG_A
Cuts at: 8361 8361
Size: 8698
BspHI T'CATG_A
Cuts at: 5034 6587 8317 5034
Size: 1553 1730 5415
Fragments arranged by size:
5415 1730 1553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 6053 844
Size: 2197 3012 3489
Fragments arranged by size:
3489 3012 2197
BsrBI CCG'CTC
Cuts at: 1394 1823 6585 1394
Size: 429 4762 3507
Fragments arranged by size:
4762 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4407 5860 7193 7367 65
Size: 1183 3159 1453 1333 174 1396
Fragments arranged by size:
3159 1453 1396 1333 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5908 7342 7529 1700
Size: 110 4098 1434 187 2869
Fragments arranged by size:
4098 2869 1434 187 110
BsrGI T'GTAC_A
Cuts at: 96 5242 96
Size: 5146 3552
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8698
BssSI C'ACGA_G
Cuts at: 6758 7770 6758
Size: 1012 7686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5201 5440 794
Size: 4407 239 4052
Fragments arranged by size:
4407 4052 239
BstBI TT'CG_AA
Cuts at: 2007 7502 2007
Size: 5495 3203
BstEII G'GTnAC_C
Cuts at: 1184 3332 1184
Size: 2148 6550
BstXI CCAn_nnnn'nTGG
Cuts at: 4132 5380 4132
Size: 1248 7450
BstYI r'GATC_y
Cuts at: 1972 2356 2387 2623 3681 4215 6028 6395 6780
Size: 384 31 236 1058 534 1813 367 385
Cuts at: 6780 6797 8249 8335 8347 8693 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1813 1452 1058 534 385 384 367
346 236 86 31 17 12
BstZ17I GTA'TAC
Cuts at: 5070 5992 5070
Size: 922 7776
Bsu36I CC'TnA_GG
Cuts at: 3000 4099 3000
Size: 1099 7599
BtgI C'CryG_G
Cuts at: 513 1065 5577 5608 5917 513
Size: 552 4512 31 309 3294
Fragments arranged by size:
4512 3294 552 309 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7959
Fragments arranged by size:
7959 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 3874 4638 4841 5438 6214 7008 7028 2101
Size: 1773 764 203 597 776 794 20 3771
Fragments arranged by size:
3771 1773 794 776 764 597 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4857 4892 529
Size: 35 4293 35 4335
Fragments arranged by size:
4335 4293 35 35
DraI TTT'AAA
Cuts at: 3063 3321 4152 4548 6002 3063
Size: 258 831 396 1454 5759
Fragments arranged by size:
5759 1454 831 396 258
DraIII CAC_nnn'GTG
Cuts at: 4714 4714
Size: 8698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7705 809
Size: 507 6389 1802
Fragments arranged by size:
6389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5857 6145 7034
Size: 54 1425 50 275 18 4027 288 889
Cuts at: 7034 8663 8
Size: 1629 43
Fragments arranged by size:
4027 1629 1425 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6145 1537
Size: 275 4333 4090
Fragments arranged by size:
4333 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4470 5356 6628 1353
Size: 3117 886 1272 3423
Fragments arranged by size:
3423 3117 1272 886
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5495 7285 7659 7805 387
Size: 1064 138 3906 1790 374 146 1280
Fragments arranged by size:
3906 1790 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4106 4113 4106
Size: 7 8691
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2724 3696 3780 4182 4437 4759 5038
Size: 393 1092 972 84 402 255 322 279
Cuts at: 5038 5798 6761 7358 8145 8413 1239
Size: 760 963 597 787 268 1524
Fragments arranged by size:
1524 1092 972 963 787 760 597 402
393 322 279 268 255 84
EcoO109I rG'GnC_Cy
Cuts at: 2590 5925 5944 5945 2590
Size: 3335 19 1 5343
Fragments arranged by size:
5343 3335 19 1
EcoRI G'AATT_C
Cuts at: 2338 2870 6007 6385 2338
Size: 532 3137 378 4651
Fragments arranged by size:
4651 3137 532 378
EcoRV GAT'ATC
Cuts at: 4699 4699
Size: 8698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2873 4442 4717 2873
Size: 1569 275 6854
Fragments arranged by size:
6854 1569 275
FspI TGC'GCA
Cuts at: 7204 7204
Size: 8698
HaeII r_GCGC'y
Cuts at: 1678 5340 5901 7845 1678
Size: 3662 561 1944 2531
Fragments arranged by size:
3662 2531 1944 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 4469 4501 5558
Size: 32 135 32 800 32 2065 32 1057
Cuts at: 5558 5590 7339 7371 7413 7445 1373
Size: 32 1749 32 42 32 2626
Fragments arranged by size:
2626 2065 1749 1057 800 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 2928 6042 6288 669
Size: 988 281 457 533 3114 246 3079
Fragments arranged by size:
3114 3079 988 533 457 281 246
HindIII A'AGCT_T
Cuts at: 748 5739 748
Size: 4991 3707
HpaI GTT'AAC
Cuts at: 2395 6288 2395
Size: 3893 4805
KpnI G_GTAC'C
Cuts at: 3809 4139 6023 3809
Size: 330 1884 6484
Fragments arranged by size:
6484 1884 330
MfeI C'AATT_G
Cuts at: 5686 6297 5686
Size: 611 8087
MluI A'CGCG_T
Cuts at: 8574 8574
Size: 8698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3016 4360 4372 4491 7812 7996 1305
Size: 1711 1344 12 119 3321 184 2007
Fragments arranged by size:
3321 2007 1711 1344 184 119 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5859 10
Size: 54 1425 343 4027 2849
Fragments arranged by size:
4027 2849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 2655 4130 6656 7015 7174 518
Size: 648 1018 471 1475 2526 359 159 2042
Fragments arranged by size:
2526 2042 1475 1018 648 471 359 159
MspA1I CmG'CkG
Cuts at: 1606 6114 6791 7939 8184 1606
Size: 4508 677 1148 245 2120
Fragments arranged by size:
4508 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 5577 5608 5917 513
Size: 552 4512 31 309 3294
Fragments arranged by size:
4512 3294 552 309 31
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8698
NotI GC'GGCC_GC
Cuts at: 6145 6145
Size: 8698
NsiI A_TGCA'T
Cuts at: 2181 4629 2181
Size: 2448 6250
NspI r_CATG'y
Cuts at: 1132 1229 2752 3888 4631 5189 5732 6056 7601
Size: 97 1523 1136 743 558 543 324 1545
Cuts at: 7601 1132
Size: 2229
Fragments arranged by size:
2229 1545 1523 1136 743 558 543 324
97
PacI TTA_AT'TAA
Cuts at: 2064 2064
Size: 8698
PasI CC'CwG_GG
Cuts at: 1348 2533 1348
Size: 1185 7513
PciI A'CATG_T
Cuts at: 7597 7597
Size: 8698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5012 5521 1108
Size: 3904 509 4285
Fragments arranged by size:
4285 3904 509
PmeI GTTT'AAAC
Cuts at: 6002 6002
Size: 8698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6767 6799 8280 8312 6767
Size: 32 1481 32 7153
Fragments arranged by size:
7153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2590 2590
Size: 8698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8698
PsiI TTA'TAA
Cuts at: 2232 6268 2232
Size: 4036 4662
PspOMI G'GGCC_C
Cuts at: 5944 5944
Size: 8698
PstI C_TGCA'G
Cuts at: 830 3881 4279 5413 5707 6050 830
Size: 3051 398 1134 294 343 3478
Fragments arranged by size:
3478 3051 1134 398 343 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8698
SacI G_AGCT'C
Cuts at: 4108 4115 4108
Size: 7 8691
SalI G'TCGA_C
Cuts at: 1936 6040 1936
Size: 4104 4594
SbfI CC_TGCA'GG
Cuts at: 6050 6050
Size: 8698
ScaI AGT'ACT
Cuts at: 4857 6946 8658 4857
Size: 2089 1712 4897
Fragments arranged by size:
4897 2089 1712
SfcI C'TryA_G
Cuts at: 826 1045 2074 2865 2984 3367 3877 3913 4275
Size: 219 1029 791 119 383 510 36 362
Cuts at: 4275 4661 5409 5562 5703 6046 7181 7862 8053
Size: 386 748 153 141 343 1135 681 191
Cuts at: 8053 826
Size: 1471
Fragments arranged by size:
1471 1135 1029 791 748 681 510 386
383 362 343 219 191 153 141 119
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8698
SmaI CCC'GGG
Cuts at: 1872 6025 1872
Size: 4153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3996 4024 5428 6802 7703 7965
Size: 197 926 2053 28 1404 1374 901 262
Cuts at: 7965 820
Size: 1553
Fragments arranged by size:
2053 1553 1404 1374 926 901 262 197
28
SnaBI TAC'GTA
Cuts at: 493 5995 6489 493
Size: 5502 494 2702
Fragments arranged by size:
5502 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8698
SphI G_CATG'C
Cuts at: 1132 4631 6056 1132
Size: 3499 1425 3774
Fragments arranged by size:
3774 3499 1425
SspI AAT'ATT
Cuts at: 5 52 3271 6622 5
Size: 47 3219 3351 2081
Fragments arranged by size:
3351 3219 2081 47
StuI AGG'CCT
Cuts at: 1517 4097 1517
Size: 2580 6118
StyI C'CwwG_G
Cuts at: 513 1065 2615 3405 4617 4789 4910 5577 5608
Size: 552 1550 790 1212 172 121 667 31
Cuts at: 5608 5901 5917 6137 513
Size: 293 16 220 3074
Fragments arranged by size:
3074 1550 1212 790 667 552 293 220
172 121 31 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2386 3773 5509 6725 6742 6895 7080
Size: 1255 806 1387 1736 1216 17 153 185
Cuts at: 7080 7525 325
Size: 445 1498
Fragments arranged by size:
1736 1498 1387 1255 1216 806 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4855 5242 5877
Size: 274 80 33 51 1443 2878 387 635
Cuts at: 5877 6944 8656 96
Size: 1067 1712 138
Fragments arranged by size:
2878 1712 1443 1067 635 387 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4155 4187 4667 4699 5796 5828 8658 8690 4155
Size: 32 480 32 1097 32 2830 32 4163
Fragments arranged by size:
4163 2830 1097 480 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4304 1316
Size: 2988 5710
XbaI T'CTAG_A
Cuts at: 3055 6034 3055
Size: 2979 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 8698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8698
XmaI C'CCGG_G
Cuts at: 1870 6023 1870
Size: 4153 4545
XmnI GAAnn'nnTTC
Cuts at: 2979 3896 5456 5603 6827 2979
Size: 917 1560 147 1224 4850
Fragments arranged by size:
4850 1560 1224 917 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6506 276
Size: 53 83 186 936 4972 2468
Fragments arranged by size:
4972 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BsiWI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI
PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI