Restriction map for
pFN21ASDB1510
(Circular) (Six-base) MAPSORT of: pFN21ASDB1510.seq Check: 7341 from: 1 to: 6472
pFN21ASDB1510 6472 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2901 3815 1937
Size: 964 914 4594
Fragments arranged by size:
4594 964 914
Acc65I G'GTAC_C
Cuts at: 2612 3793 2612
Size: 1181 5291
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2958 3205 3599 3608 4535 5919 6187 1632
Size: 1326 247 394 9 927 1384 268 1917
Fragments arranged by size:
1917 1384 1326 927 394 268 247 9
AfeI AGC'GCT
Cuts at: 2070 2070
Size: 6472
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2606 3224 1184
Size: 1422 618 4432
Fragments arranged by size:
4432 1422 618
AlwNI CAG_nnn'CTG
Cuts at: 2041 2566 2743 3061 3228 4215 5787 2041
Size: 525 177 318 167 987 1572 2726
Fragments arranged by size:
2726 1572 987 525 318 177 167
ApaI G_GGCC'C
Cuts at: 2129 2306 2656 2896 2129
Size: 177 350 240 5705
Fragments arranged by size:
5705 350 240 177
ApaLI G'TGCA_C
Cuts at: 2619 4529 5685 2619
Size: 1910 1156 3406
Fragments arranged by size:
3406 1910 1156
ApoI r'AATT_y
Cuts at: 1493 3781 4007 4159 6268 1493
Size: 2288 226 152 2109 1697
Fragments arranged by size:
2288 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2204 3446 3522 3797 1870
Size: 73 261 1242 76 275 4545
Fragments arranged by size:
4545 1242 275 261 76 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2612 3793 5248 618
Size: 325 217 627 825 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 825 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2129 2205 2211 2306 2647 2656 2896 3791
Size: 738 76 6 95 341 9 240 895
Cuts at: 3791 1391
Size: 4072
Fragments arranged by size:
4072 895 738 341 240 95 76 9
6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2045 2079 2446 2456 2480 2490 4663
Size: 34 108 34 367 10 24 10 2173
Cuts at: 4663 4697 1903
Size: 34 3678
Fragments arranged by size:
3678 2173 367 108 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 2310 5083
Size: 107 122 71 631 605 580 58 2773
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
2773 1525 631 605 580 122 107 71
58
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3429 3889 3429
Size: 460 6012
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2306 2623 2656 2896 3487 4533 5689
Size: 339 177 317 33 240 591 1046 1156
Cuts at: 5689 1790
Size: 2573
Fragments arranged by size:
2573 1156 1046 591 339 317 240 177
33
BmgBI CAC'GTC
Cuts at: 1318 3466 1318
Size: 2148 4324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5161 442
Size: 416 53 83 399 24 2786 958 1753
Fragments arranged by size:
2786 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2588 2692 3444 3476 3728 5132 1239
Size: 1262 87 104 752 32 252 1404 2579
Fragments arranged by size:
2579 1404 1262 752 252 104 87 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 5462 5760 4597
Size: 865 298 5309
Fragments arranged by size:
5309 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 3099 3505 5135 882
Size: 617 893 707 406 1630 2219
Fragments arranged by size:
2219 1630 893 707 617 406
BsaAI yAC'GTr
Cuts at: 493 1204 3235 3769 4263 493
Size: 711 2031 534 494 2702
Fragments arranged by size:
2702 2031 711 534 494
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4279 4661 5296 275
Size: 53 83 186 936 2746 382 635 1451
Fragments arranged by size:
2746 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6247
Fragments arranged by size:
6247 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3605 3728 3731 1186
Size: 1312 1107 123 3 3927
Fragments arranged by size:
3927 1312 1107 123 3
BseYI C'CCAG_C
Cuts at: 2041 2059 2163 2344 2749 2771 3023 5675 6171
Size: 18 104 181 405 22 252 2652 496
Cuts at: 6171 2041
Size: 2342
Fragments arranged by size:
2652 2342 496 405 252 181 104 22
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3423 1163
Size: 573 1687 4212
Fragments arranged by size:
4212 1687 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 2123 2314 3630 3922 4683
Size: 275 186 100 22 191 1316 292 761
Cuts at: 4683 5711 1540
Size: 1028 2301
Fragments arranged by size:
2301 1316 1028 761 292 275 191 186
100 22
BsiHKAI G_wGCw'C
Cuts at: 2585 2623 2647 4533 4618 5689 2585
Size: 38 24 1886 85 1071 3368
Fragments arranged by size:
3368 1886 1071 85 38 24
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2452 2225
Size: 227 6245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2205 2211 2306 2585 2623 2647
Size: 399 339 76 6 95 279 38 24
Cuts at: 2647 2656 2896 3487 3791 4533 4618 5689 1391
Size: 9 240 591 304 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 399 339 304 279
240 95 85 76 38 24 9 6
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2690 3445 3827 844
Size: 1846 755 382 3489
Fragments arranged by size:
3489 1846 755 382
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2552 4967 5141 65
Size: 1183 1304 2415 174 1396
Fragments arranged by size:
2415 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2376 5116 5303 1700
Size: 110 566 2740 187 2869
Fragments arranged by size:
2869 2740 566 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 2455 4532 5544 2455
Size: 2077 1012 3383
Fragments arranged by size:
3383 2077 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 2007 5276 2007
Size: 3269 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstXI CCAn_nnnn'nTGG
Cuts at: 3640 3640
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 2403 3802 4169 4554 4571 6023 6109 6121
Size: 431 1399 367 385 17 1452 86 12
Cuts at: 6121 6467 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1399 431 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2047 2146 2950 3279 513
Size: 552 957 25 99 804 329 3706
Fragments arranged by size:
3706 957 804 552 329 99 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3079 3331 487
Size: 683 56 1853 252 3628
Fragments arranged by size:
3628 1853 683 252 56
BtsI GCAGTG_nn'
Cuts at: 2527 3309 3342 3440 3485 3988 4782 4802 2527
Size: 782 33 98 45 503 794 20 4197
Fragments arranged by size:
4197 794 782 503 98 45 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2120 2244
Size: 54 1425 50 275 18 268 22 124
Cuts at: 2244 2311 2670 2953 3385 3627 3642 3919 4808
Size: 67 359 283 432 242 15 277 889
Cuts at: 4808 6437 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 432 359 283 277 275
268 242 124 67 54 50 43 22
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2098 2120 2311 3627 3919 1537
Size: 275 286 22 191 1316 292 4090
Fragments arranged by size:
4090 1316 292 286 275 191 22
EarI CTCTTCn'nnn_
Cuts at: 1353 2875 4402 1353
Size: 1522 1527 3423
Fragments arranged by size:
3423 1527 1522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 2384 3261 5059 5433 5579
Size: 1064 138 633 162 877 1798 374 146
Cuts at: 5579 387
Size: 1280
Fragments arranged by size:
1798 1280 1064 877 633 374 162 146
138
EcoICRI GAG'CTC
Cuts at: 2645 2645
Size: 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2588 2692 2958 3205 3444 3476
Size: 393 869 87 104 266 247 239 32
Cuts at: 3476 3599 3608 3728 4535 5132 5919 6187 1239
Size: 123 9 120 807 597 787 268 1524
Fragments arranged by size:
1524 869 807 787 597 393 268 266
247 239 123 120 104 87 32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 6472
EcoO109I rG'GnC_Cy
Cuts at: 2303 2438 2652 2653 2813 2892 2893 2303
Size: 135 214 1 160 79 1 5882
Fragments arranged by size:
5882 214 160 135 79 1 1
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2042 2072 5619 1678
Size: 364 30 3547 2531
Fragments arranged by size:
3547 2531 364 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3098 3130 5113 5145 5187
Size: 32 135 32 1526 32 1983 32 42
Cuts at: 5187 5219 1373
Size: 32 2626
Fragments arranged by size:
2626 1983 1526 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2902 3816 4062 669
Size: 988 281 964 914 246 3079
Fragments arranged by size:
3079 988 964 914 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 2616 3797 2616
Size: 1181 5291
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 3141 3643 5586 5770 1305
Size: 1125 711 502 1943 184 2007
Fragments arranged by size:
2007 1943 1125 711 502 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 3644 10
Size: 54 1425 343 840 972 2838
Fragments arranged by size:
2838 1425 972 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2678 3128 4430 4789 4948 518
Size: 648 861 651 450 1302 359 159 2042
Fragments arranged by size:
2042 1302 861 651 648 450 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2059 2148 2269 2881 3058 3228 3888
Size: 443 10 89 121 612 177 170 660
Cuts at: 3888 4565 5713 5958 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 612 443 245 177
170 121 89 10
NaeI GCC'GGC
Cuts at: 2378 2378
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 2022 2950 513
Size: 552 957 928 4035
Fragments arranged by size:
4035 957 928 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NgoMIV G'CCGG_C
Cuts at: 2376 2376
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3830 5375 1132
Size: 97 2601 1545 2229
Fragments arranged by size:
2601 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3297 1348
Size: 1949 4523
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2566 2743 1108
Size: 1134 324 177 4837
Fragments arranged by size:
4837 1134 324 177
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2813 2813
Size: 6472
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 2125 2302 2652 2892 2125
Size: 177 350 240 5705
Fragments arranged by size:
5705 350 240 177
PstI C_TGCA'G
Cuts at: 830 2058 2522 3057 3213 3824 830
Size: 1228 464 535 156 611 3478
Fragments arranged by size:
3478 1228 611 535 464 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2059 3058 3228 2059
Size: 999 170 5303
Fragments arranged by size:
5303 999 170
SacI G_AGCT'C
Cuts at: 2647 2647
Size: 6472
SacII CC_GC'GG
Cuts at: 2050 2149 2050
Size: 99 6373
SalI G'TCGA_C
Cuts at: 1936 2900 3814 1936
Size: 964 914 4594
Fragments arranged by size:
4594 964 914
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2054 2518 3053 3209 3313 3820 4955
Size: 219 1009 464 535 156 104 507 1135
Cuts at: 4955 5636 5827 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 535 507 464 219
191 156 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 2310 2310
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3799 1872
Size: 1927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4576 5477 5739 820
Size: 197 926 2633 901 262 1553
Fragments arranged by size:
2633 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3769 4263 493
Size: 3276 494 2702
Fragments arranged by size:
3276 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 2943 1517
Size: 1426 5046
StyI C'CwwG_G
Cuts at: 513 1065 2022 2667 2766 2950 3911 513
Size: 552 957 645 99 184 961 3074
Fragments arranged by size:
3074 961 957 645 552 184 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3037 4499 4516 4669 4854 5299 325
Size: 1255 1457 1462 17 153 185 445 1498
Fragments arranged by size:
1498 1462 1457 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4718 6430 96
Size: 274 80 33 51 1443 2741 1712 138
Fragments arranged by size:
2741 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3271 3303 6432 6464 3271
Size: 32 3129 32 3279
Fragments arranged by size:
3279 3129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3797 1870
Size: 1927 4545
XmnI GAAnn'nnTTC
Cuts at: 3567 4601 3567
Size: 1034 5438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI
SwaI XcmI