Restriction map for
pFN21ASDB1496
(Circular) (Six-base) MAPSORT of: pFN21ASDB1496.seq Check: 8055 from: 1 to: 5560
pFN21ASDB1496 5560 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2579 2579
Size: 5560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3623 5007 5275 1632
Size: 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2622 3303 4875 2622
Size: 681 1572 3307
Fragments arranged by size:
3307 1572 681
ApaI G_GGCC'C
Cuts at: 2065 2275 2065
Size: 210 5350
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2843 2881 4336 618
Size: 325 217 627 1056 38 1455 1842
Fragments arranged by size:
1842 1455 1056 627 325 217 38
BanII G_rGCy'C
Cuts at: 1391 2065 2275 2879 1391
Size: 674 210 604 4072
Fragments arranged by size:
4072 674 604 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BbvCI CC'TCA_GC
Cuts at: 2286 2286
Size: 5560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 5555 2319
Size: 3236 2324
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2065 2275 3621 4777 1790
Size: 275 210 1346 1156 2573
Fragments arranged by size:
2573 1346 1156 275 210
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
Bpu10I CC'TnA_GC
Cuts at: 2286 2360 2286
Size: 74 5486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 4223 882
Size: 617 660 2064 2219
Fragments arranged by size:
2219 2064 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 2857 3351 493
Size: 711 1653 494 2702
Fragments arranged by size:
2702 1653 711 494
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 1178
Size: 30 165 30 1017 30 4288
Fragments arranged by size:
4288 1017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5560
BseYI C'CCAG_C
Cuts at: 2591 4763 5259 2591
Size: 2172 496 2892
Fragments arranged by size:
2892 2172 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3621 3706 4777 3621
Size: 85 1071 4404
Fragments arranged by size:
4404 1071 85
BsmI GAATG_Cn'
Cuts at: 2665 3071 3164 2665
Size: 406 93 5061
Fragments arranged by size:
5061 406 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2275 2879 3621 3706 4777 1391
Size: 399 275 210 604 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 275 210 85
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 2793 3449 5179 2793
Size: 656 1730 3174
Fragments arranged by size:
3174 1730 656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2579 2915 844
Size: 1735 336 3489
Fragments arranged by size:
3489 1735 336
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 3447 1394
Size: 429 608 1016 3507
Fragments arranged by size:
3507 1016 608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2303 4204 4391 1700
Size: 110 493 1901 187 2869
Fragments arranged by size:
2869 1901 493 187 110
BsrGI T'GTAC_A
Cuts at: 96 2544 96
Size: 2448 3112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 2007 4364 2007
Size: 2357 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2319 2890 3257 3642 3659 5111 5197 5209
Size: 347 571 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 571 385 367 347 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2654 513
Size: 552 957 632 3419
Fragments arranged by size:
3419 957 632 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2662 2697 529
Size: 35 2098 35 3392
Fragments arranged by size:
3392 2098 35 35
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 3007 3896
Size: 54 1425 50 275 18 252 925 889
Cuts at: 3896 5525 8
Size: 1629 43
Fragments arranged by size:
1629 1425 925 889 275 252 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3490 1353
Size: 2137 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3623 4220 5007 5275 1239
Size: 393 1991 597 787 268 1524
Fragments arranged by size:
1991 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2061 2062 2271 2272 2356 2061
Size: 1 209 1 84 5265
Fragments arranged by size:
5265 209 84 1 1
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2712 4674 4858 1305
Size: 1407 1962 184 2007
Fragments arranged by size:
2007 1962 1407 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 10
Size: 54 1425 343 252 3486
Fragments arranged by size:
3486 1425 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2792 3518 3877 4036 518
Size: 648 1044 582 726 359 159 2042
Fragments arranged by size:
2042 1044 726 648 582 359 159
MspA1I CmG'CkG
Cuts at: 1606 2714 2976 3653 4801 5046 1606
Size: 1108 262 677 1148 245 2120
Fragments arranged by size:
2120 1148 1108 677 262 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2654 513
Size: 552 957 632 3419
Fragments arranged by size:
3419 957 632 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PspOMI G'GGCC_C
Cuts at: 2061 2271 2061
Size: 210 5350
PstI C_TGCA'G
Cuts at: 830 2767 2912 830
Size: 1937 145 3478
Fragments arranged by size:
3478 1937 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
PvuII CAG'CTG
Cuts at: 2714 2714
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2763 2908 4043 4724 4915 826
Size: 219 1718 145 1135 681 191 1471
Fragments arranged by size:
1718 1471 1135 681 219 191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 2303 1700
Size: 603 4957
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2857 3351 493
Size: 2364 494 2702
Fragments arranged by size:
2702 2364 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2022 2654 2999 513
Size: 552 957 632 345 3074
Fragments arranged by size:
3074 957 632 552 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3587 3604 3757 3942 4387 325
Size: 1255 2007 17 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 3806 5518
Size: 274 80 33 51 1443 567 1262 1712
Cuts at: 5518 96
Size: 138
Fragments arranged by size:
1712 1443 1262 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 5520 5552 2420
Size: 32 3068 32 2428
Fragments arranged by size:
3068 2428 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 2663 3689 2663
Size: 1026 4534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI