Restriction map for
pFN21ASDB1489
(Circular) (Six-base) MAPSORT of: pFN21ASDB1489.seq Check: 567 from: 1 to: 9283
pFN21ASDB1489 9283 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2239 2770 3661 3868 3909 2239
Size: 531 891 207 41 7613
Fragments arranged by size:
7613 891 531 207 41
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7093 278
Size: 53 83 186 936 5557 2468
Fragments arranged by size:
5557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6626 1937
Size: 4689 4594
Acc65I G'GTAC_C
Cuts at: 6604 6604
Size: 9283
AclI AA'CG_TT
Cuts at: 1563 7410 7783 1563
Size: 5847 373 3063
Fragments arranged by size:
5847 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6018 7346 8730 8998 1632
Size: 4386 1328 1384 268 1917
Fragments arranged by size:
4386 1917 1384 1328 268
AfeI AGC'GCT
Cuts at: 3812 4931 3812
Size: 1119 8164
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9086
AflIII A'CryG_T
Cuts at: 1203 3290 5796 8182 9159 1203
Size: 2087 2506 2386 977 1327
Fragments arranged by size:
2506 2386 2087 1327 977
AgeI A'CCGG_T
Cuts at: 8114 8114
Size: 9283
AhdI GACnn_n'nnGTC
Cuts at: 8012 8012
Size: 9283
AleI CACnn'nnGTG
Cuts at: 1166 3785 5420 1166
Size: 2619 1635 5029
Fragments arranged by size:
5029 2619 1635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4666 4676 1184
Size: 3482 10 5791
Fragments arranged by size:
5791 3482 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4183 4215 4183
Size: 32 9251
AlwNI CAG_nnn'CTG
Cuts at: 2103 2631 3467 3512 4244 4355 4510 4552 5022
Size: 528 836 45 732 111 155 42 470
Cuts at: 5022 5539 5861 7026 8598 2103
Size: 517 322 1165 1572 2788
Fragments arranged by size:
2788 1572 1165 836 732 528 517 470
322 155 111 45 42
ApaI G_GGCC'C
Cuts at: 2753 3110 3173 4187 5069 5687 5950 6050 2753
Size: 357 63 1014 882 618 263 100 5986
Fragments arranged by size:
5986 1014 882 618 357 263 100 63
ApaLI G'TGCA_C
Cuts at: 2909 3896 4807 5423 6388 7340 8496 2909
Size: 987 911 616 965 952 1156 3696
Fragments arranged by size:
3696 1156 987 965 952 911 616
ApoI r'AATT_y
Cuts at: 1493 6592 6818 6970 9079 1493
Size: 5099 226 152 2109 1697
Fragments arranged by size:
5099 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7837 160
Size: 872 6805 1606
Fragments arranged by size:
6805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9283
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2173 2327 2744 2782 3210 3227
Size: 73 176 54 154 417 38 428 17
Cuts at: 3227 4338 4785 6031 6128 6361 6498 6608 1870
Size: 1111 447 1246 97 233 137 110 4545
Fragments arranged by size:
4545 1246 1111 447 428 417 233 176
154 137 110 97 73 54 38 17
BamHI G'GATC_C
Cuts at: 4432 6613 6980 4432
Size: 2181 367 6735
Fragments arranged by size:
6735 2181 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2506 2740 3194 3362 4195
Size: 325 217 627 719 234 454 168 833
Cuts at: 4195 5184 5731 6438 6604 8059 618
Size: 989 547 707 166 1455 1842
Fragments arranged by size:
1842 1455 989 833 719 707 627 547
454 325 234 217 168 166
BanII G_rGCy'C
Cuts at: 1391 2484 2753 3110 3173 3965 4011 4187 4389
Size: 1093 269 357 63 792 46 176 202
Cuts at: 4389 4407 4613 5069 5291 5667 5687 5950 6050
Size: 18 206 456 222 376 20 263 100
Cuts at: 6050 6194 6285 6482 6540 6602 1391
Size: 144 91 197 58 62 4072
Fragments arranged by size:
4072 1093 792 456 376 357 269 263
222 206 202 197 176 144 100 91
63 62 58 46 20 18
BbeI G_GCGC'C
Cuts at: 2510 6442 2510
Size: 3932 5351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5107 5380 5505 928
Size: 971 3208 273 125 4706
Fragments arranged by size:
4706 3208 971 273 125
BbvCI CC'TCA_GC
Cuts at: 2766 5699 2766
Size: 2933 6350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3208 3242 4900 4934 7474 7508 1903
Size: 34 1271 34 1658 34 2540 34 3678
Fragments arranged by size:
3678 2540 1658 1271 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4997 7175 8385 4997
Size: 2178 1210 5895
Fragments arranged by size:
5895 2178 1210
BclI T'GATC_A
Cuts at: 1548 3990 1548
Size: 2442 6841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2627 4059 4315
Size: 107 122 71 631 605 955 1432 256
Cuts at: 4315 5339 5682 5691 5945 7894 136
Size: 1024 343 9 254 1949 1525
Fragments arranged by size:
1949 1525 1432 1024 955 631 605 343
256 254 122 107 71 9
BglII A'GATC_T
Cuts at: 9278 9278
Size: 9283
BlpI GC'TnA_GC
Cuts at: 2478 2643 3873 4118 4383 4793 5215 5246 5251
Size: 165 1230 245 265 410 422 31 5
Cuts at: 5251 6700 2478
Size: 1449 5061
Fragments arranged by size:
5061 1449 1230 422 410 265 245 165
31 5
Bme1580I G_kGCm'C
Cuts at: 1790 2745 2753 2913 3000 3110 3173 3199 3236
Size: 955 8 160 87 110 63 26 37
Cuts at: 3236 3341 3365 3494 3541 3792 3900 4187 4811
Size: 105 24 129 47 251 108 287 624
Cuts at: 4811 5069 5189 5427 5630 5687 5736 5950 6050
Size: 258 120 238 203 57 49 214 100
Cuts at: 6050 6129 6171 6392 7344 8500 1790
Size: 79 42 221 952 1156 2573
Fragments arranged by size:
2573 1156 955 952 624 287 258 251
238 221 214 203 160 129 120 110
108 105 100 87 79 63 57 49
47 42 37 26 24 8
BmgBI CAC'GTC
Cuts at: 1318 3120 3291 3450 1318
Size: 1802 171 159 7151
Fragments arranged by size:
7151 1802 171 159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2091 2854 3565
Size: 416 53 83 399 24 674 763 711
Cuts at: 3565 4081 4495 6485 7014 7972 442
Size: 516 414 1990 529 958 1753
Fragments arranged by size:
1990 1753 958 763 711 674 529 516
416 414 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4030 5875 4030
Size: 1845 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2617 2685 2959 4362 4444 4532 4619
Size: 974 404 68 274 1403 82 88 87
Cuts at: 4619 5057 5305 6050 6218 6472 7943 1239
Size: 438 248 745 168 254 1471 2579
Fragments arranged by size:
2579 1471 1403 974 745 438 404 274
254 248 168 88 87 82 68
Bpu10I CC'TnA_GC
Cuts at: 2419 2766 3033 4477 5699 2419
Size: 347 267 1444 1222 6003
Fragments arranged by size:
6003 1444 1222 347 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3932 7408 8273 8571 3932
Size: 3476 865 298 4644
Fragments arranged by size:
4644 3476 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 7946 882
Size: 617 3009 3438 2219
Fragments arranged by size:
3438 3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3436 3787 5135 6580 7074 493
Size: 711 2232 351 1348 1445 494 2702
Fragments arranged by size:
2702 2232 1445 1348 711 494 351
BsaBI GATnn'nnATC
Cuts at: 5562 9277 5562
Size: 3715 5568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2507 6439 7090 7472
Size: 53 83 186 936 974 3932 651 382
Cuts at: 7472 8107 275
Size: 635 1451
Fragments arranged by size:
3932 1451 974 936 651 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3365 6550 7716 8114 8388 8535 8946 3365
Size: 3185 1166 398 274 147 411 3702
Fragments arranged by size:
3702 3185 1166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2683 2713 2918
Size: 30 165 30 834 30 416 30 205
Cuts at: 2918 2948 3360 3390 4183 4213 4360 4390 5654
Size: 30 412 30 793 30 147 30 1264
Cuts at: 5654 5684 6332 6362 1178
Size: 30 648 30 4099
Fragments arranged by size:
4099 1264 834 793 648 416 412 205
165 147 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2390 2393 2618 2843 2939 3386 3521
Size: 1159 45 3 225 225 96 447 135
Cuts at: 3521 4117 4220 4837 5195 5675 1186
Size: 596 103 617 358 480 4794
Fragments arranged by size:
4794 1159 617 596 480 447 358 225
225 135 103 96 45 3
BseYI C'CCAG_C
Cuts at: 2029 2752 3003 3172 3566 4234 4371 4449 4575
Size: 723 251 169 394 668 137 78 126
Cuts at: 4575 4857 4947 5234 5755 6008 6043 6110 6401
Size: 282 90 287 521 253 35 67 291
Cuts at: 6401 6419 8486 8982 2029
Size: 18 2067 496 2330
Fragments arranged by size:
2330 2067 723 668 521 496 394 291
287 282 253 251 169 137 126 90
78 67 35 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2423 2431 2480 3178 3238 3442 3827
Size: 573 687 8 49 698 60 204 385
Cuts at: 3827 4662 4814 6500 1163
Size: 835 152 1686 3946
Fragments arranged by size:
3946 1686 835 698 687 573 385 204
152 60 49 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5954 6310 6733 7494 8522 1540
Size: 275 186 3953 356 423 761 1028 2301
Fragments arranged by size:
3953 2301 1028 761 423 356 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2589 2913 2951 3284 3627 3900 3965 4119
Size: 459 324 38 333 343 273 65 154
Cuts at: 4119 4407 4811 5291 5427 6285 6392 6458 7344
Size: 288 404 480 136 858 107 66 886
Cuts at: 7344 7429 8500 2130
Size: 85 1071 2913
Fragments arranged by size:
2913 1071 886 858 480 459 404 343
333 324 288 273 154 136 107 85
66 65 38
BsmI GAATG_Cn'
Cuts at: 3271 3505 3996 6262 6794 6887 3271
Size: 234 491 2266 532 93 5667
Fragments arranged by size:
5667 2266 532 491 234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4018 4018
Size: 9283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2484 2589 2745 2753 2913 2951
Size: 399 340 354 105 156 8 160 38
Cuts at: 2951 3000 3110 3173 3199 3236 3284 3341 3365
Size: 49 110 63 26 37 48 57 24
Cuts at: 3365 3494 3541 3627 3792 3900 3965 4011 4119
Size: 129 47 86 165 108 65 46 108
Cuts at: 4119 4187 4389 4407 4613 4811 5069 5189 5291
Size: 68 202 18 206 198 258 120 102
Cuts at: 5291 5427 5630 5667 5687 5736 5950 6050 6129
Size: 136 203 37 20 49 214 100 79
Cuts at: 6129 6171 6194 6285 6392 6458 6482 6540 6602
Size: 42 23 91 107 66 24 58 62
Cuts at: 6602 7344 7429 8500 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 340 258 214
206 203 202 198 165 160 156 136
129 120 110 108 108 107 105 102
100 91 86 85 79 68 66 65
63 62 58 57 49 49 48 47
46 42 38 37 37 26 24 24
23 20 18 8
BspEI T'CCGG_A
Cuts at: 8946 8946
Size: 9283
BspHI T'CATG_A
Cuts at: 7172 8902 7172
Size: 1730 7553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2235 2239 2318 2770 2999 3661 3735 3868
Size: 1391 4 79 452 229 662 74 133
Cuts at: 3868 3909 6638 844
Size: 41 2729 3489
Fragments arranged by size:
3489 2729 1391 662 452 229 133 79
74 41 4
BsrBI CCG'CTC
Cuts at: 1394 1823 5182 5228 6379 7170 1394
Size: 429 3359 46 1151 791 3507
Fragments arranged by size:
3507 3359 1151 791 429 46
BsrDI GCAATG_nn'
Cuts at: 65 1248 2800 4438 7778 7952 65
Size: 1183 1552 1638 3340 174 1396
Fragments arranged by size:
3340 1638 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2621 2678 7927 8114 1700
Size: 110 811 57 5249 187 2869
Fragments arranged by size:
5249 2869 811 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9283
BssSI C'ACGA_G
Cuts at: 4772 5512 5678 6278 7343 8355 4772
Size: 740 166 600 1065 1012 5700
Fragments arranged by size:
5700 1065 1012 740 600 166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2264 2631 2908 3021 3646 3676 794
Size: 1470 367 277 113 625 30 6401
Fragments arranged by size:
6401 1470 625 367 277 113 30
BstBI TT'CG_AA
Cuts at: 2007 8087 2007
Size: 6080 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9283
BstXI CCAn_nnnn'nTGG
Cuts at: 2737 5986 6187 2737
Size: 3249 201 5833
Fragments arranged by size:
5833 3249 201
BstYI r'GATC_y
Cuts at: 1972 4432 5535 5563 6059 6613 6980 7365 7382
Size: 2460 1103 28 496 554 367 385 17
Cuts at: 7382 8834 8920 8932 9278 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2460 1977 1452 1103 554 496 385 367
346 86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 3505 5688 3505
Size: 2183 7100
BtgI C'CryG_G
Cuts at: 513 1065 3260 3333 4428 5271 5736 6021 6327
Size: 552 2195 73 1095 843 465 285 306
Cuts at: 6327 513
Size: 3469
Fragments arranged by size:
3469 2195 1095 843 552 465 306 285
73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2152 3954 487
Size: 683 56 926 1802 5816
Fragments arranged by size:
5816 1802 926 683 56
BtsI GCAGTG_nn'
Cuts at: 4650 5415 5425 5711 6799 7593 7613 4650
Size: 765 10 286 1088 794 20 6320
Fragments arranged by size:
6320 1088 794 765 286 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9248
DraI TTT'AAA
Cuts at: 6587 6587
Size: 9283
DraIII CAC_nnn'GTG
Cuts at: 2917 3934 2917
Size: 1017 8266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8290 809
Size: 507 6974 1802
Fragments arranged by size:
6974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2366 2611
Size: 54 1425 50 275 18 243 293 245
Cuts at: 2611 2864 3237 3630 4326 4971 5259 5951 6330
Size: 253 373 393 696 645 288 692 379
Cuts at: 6330 6730 7619 9248 8
Size: 400 889 1629 43
Fragments arranged by size:
1629 1425 889 696 692 645 400 393
379 373 293 288 275 253 245 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5951 6730 1537
Size: 275 4139 779 4090
Fragments arranged by size:
4139 4090 779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 2832 3389 3737 4103 5372 7213 1353
Size: 1014 465 557 348 366 1269 1841 3423
Fragments arranged by size:
3423 1841 1269 1014 557 465 366 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2363 4918 5757 5905 7870 8244
Size: 1064 138 774 2555 839 148 1965 374
Cuts at: 8244 8390 387
Size: 146 1280
Fragments arranged by size:
2555 1965 1280 1064 839 774 374 148
146 138
EcoICRI GAG'CTC
Cuts at: 3963 4405 5289 6283 3963
Size: 442 884 994 6963
Fragments arranged by size:
6963 994 884 442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2617 2685 2959 4362 4444 4532
Size: 393 581 404 68 274 1403 82 88
Cuts at: 4532 4619 5057 5305 6018 6050 6218 6472 7346
Size: 87 438 248 713 32 168 254 874
Cuts at: 7346 7943 8730 8998 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1403 874 787 713 597 581 438
404 393 274 268 254 248 168 88
87 82 68 32
EcoNI CCTnn'n_nnAGG
Cuts at: 3632 3772 4106 4206 4693 5260 5369 5651 3632
Size: 140 334 100 487 567 109 282 7264
Fragments arranged by size:
7264 567 487 334 282 140 109 100
EcoO109I rG'GnC_Cy
Cuts at: 2136 2152 2423 2491 2628 2705 2749 2934 2970
Size: 16 271 68 137 77 44 185 36
Cuts at: 2970 3029 3098 3106 3135 3724 4183 4317 4588
Size: 59 69 8 29 589 459 134 271
Cuts at: 4588 5066 5374 5684 5946 5961 6047 2136
Size: 478 308 310 262 15 86 5372
Fragments arranged by size:
5372 589 478 459 310 308 271 271
262 185 137 134 86 77 69 68
59 44 36 29 16 15 8
EcoRI G'AATT_C
Cuts at: 6592 6970 6592
Size: 378 8905
FspI TGC'GCA
Cuts at: 2257 3680 4910 7789 2257
Size: 1423 1230 2879 3751
Fragments arranged by size:
3751 2879 1423 1230
FspAI rTGC'GCAy
Cuts at: 2257 4910 2257
Size: 2653 6630
HaeII r_GCGC'y
Cuts at: 1678 2510 3313 3814 4228 4661 4933 5318 6117
Size: 832 803 501 414 433 272 385 799
Cuts at: 6117 6418 6442 8430 1678
Size: 301 24 1988 2531
Fragments arranged by size:
2531 1988 832 803 799 501 433 414
385 301 272 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 2918 2950 3650
Size: 32 135 32 1193 32 121 32 700
Cuts at: 3650 3682 3996 4028 4238 4270 4417 4449 4460
Size: 32 314 32 210 32 147 32 11
Cuts at: 4460 4492 5129 5161 5654 5686 6142 6174 7924
Size: 32 637 32 493 32 456 32 1750
Cuts at: 7924 7956 7998 8030 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 1193 700 637 493 456 314
210 147 135 121 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 5599 6627 6873 669
Size: 988 281 364 3297 1028 246 3079
Fragments arranged by size:
3297 3079 1028 988 364 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9283
HpaI GTT'AAC
Cuts at: 6873 6873
Size: 9283
KasI G'GCGC_C
Cuts at: 2506 6438 2506
Size: 3932 5351
KpnI G_GTAC'C
Cuts at: 6608 6608
Size: 9283
MfeI C'AATT_G
Cuts at: 6882 6882
Size: 9283
MluI A'CGCG_T
Cuts at: 9159 9159
Size: 9283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 4782 5157 5172 6115 8397 8581 1305
Size: 1077 2400 375 15 943 2282 184 2007
Fragments arranged by size:
2400 2282 2007 1077 943 375 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 3632 10
Size: 54 1425 343 243 1557 5661
Fragments arranged by size:
5661 1557 1425 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3785 4397 5324 5420 6185 7241 7600
Size: 648 2619 612 927 96 765 1056 359
Cuts at: 7600 7759 518
Size: 159 2042
Fragments arranged by size:
2619 2042 1056 927 765 648 612 359
159 96
MspA1I CmG'CkG
Cuts at: 1606 2396 2415 2464 2513 2595 2658 2958 3464
Size: 790 19 49 49 82 63 300 506
Cuts at: 3464 3525 4019 4324 4382 4449 4536 5530 5824
Size: 61 494 305 58 67 87 994 294
Cuts at: 5824 6699 7376 8524 8769 1606
Size: 875 677 1148 245 2120
Fragments arranged by size:
2120 1148 994 875 790 677 506 494
305 300 294 245 87 82 67 63
61 58 49 49 19
NaeI GCC'GGC
Cuts at: 2623 2680 2623
Size: 57 9226
NarI GG'CG_CC
Cuts at: 2507 6439 2507
Size: 3932 5351
NcoI C'CATG_G
Cuts at: 513 1065 3260 5271 5736 513
Size: 552 2195 2011 465 4060
Fragments arranged by size:
4060 2195 2011 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9283
NgoMIV G'CCGG_C
Cuts at: 2621 2678 2621
Size: 57 9226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9283
NotI GC'GGCC_GC
Cuts at: 6730 6730
Size: 9283
NspI r_CATG'y
Cuts at: 1132 1229 3189 3325 3649 3667 5098 5800 6641
Size: 97 1960 136 324 18 1431 702 841
Cuts at: 6641 8186 1132
Size: 1545 2229
Fragments arranged by size:
2229 1960 1545 1431 841 702 324 136
97 18
PasI CC'CwG_GG
Cuts at: 1348 3102 4133 5190 5943 6407 1348
Size: 1754 1031 1057 753 464 4224
Fragments arranged by size:
4224 1754 1057 1031 753 464
PciI A'CATG_T
Cuts at: 5796 8182 5796
Size: 2386 6897
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2296 3547 4510 5455 5717 6554 1108
Size: 1188 1251 963 945 262 837 3837
Fragments arranged by size:
3837 1251 1188 963 945 837 262
PfoI T'CCnGG_A
Cuts at: 2183 2936 2972 5448 2183
Size: 753 36 2476 6018
Fragments arranged by size:
6018 2476 753 36
PmeI GTTT'AAAC
Cuts at: 6587 6587
Size: 9283
PmlI CAC'GTG
Cuts at: 5135 5135
Size: 9283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4183 4215 7352 7384 8865 8897 4183
Size: 32 3137 32 1481 32 4569
Fragments arranged by size:
4569 3137 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2934 2970 3029 3098 3135 3724 4588 5961
Size: 782 36 59 69 37 589 864 1373
Cuts at: 5961 2152
Size: 5474
Fragments arranged by size:
5474 1373 864 782 589 69 59 37
36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9283
PsiI TTA'TAA
Cuts at: 6853 6853
Size: 9283
PspOMI G'GGCC_C
Cuts at: 2749 3106 3169 4183 5065 5683 5946 6046 2749
Size: 357 63 1014 882 618 263 100 5986
Fragments arranged by size:
5986 1014 882 618 357 263 100 63
PspXI vC'TCGA_Gb
Cuts at: 3227 3227
Size: 9283
PstI C_TGCA'G
Cuts at: 830 2151 2657 2878 3042 3056 3690 5074 5535
Size: 1321 506 221 164 14 634 1384 461
Cuts at: 5535 5907 6635 830
Size: 372 728 3478
Fragments arranged by size:
3478 1384 1321 728 634 506 461 372
221 164 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9283
PvuII CAG'CTG
Cuts at: 2464 2658 2958 3464 4019 4382 4449 5530 5824
Size: 194 300 506 555 363 67 1081 294
Cuts at: 5824 2464
Size: 5923
Fragments arranged by size:
5923 1081 555 506 363 300 294 194
67
SacI G_AGCT'C
Cuts at: 3965 4407 5291 6285 3965
Size: 442 884 994 6963
Fragments arranged by size:
6963 994 884 442
SalI G'TCGA_C
Cuts at: 1936 6625 1936
Size: 4689 4594
SanDI GG'GwC_CC
Cuts at: 2970 3098 3135 5961 2970
Size: 128 37 2826 6292
Fragments arranged by size:
6292 2826 128 37
SapI GCTCTTCn'nnn_
Cuts at: 3737 3737
Size: 9283
SbfI CC_TGCA'GG
Cuts at: 3690 6635 3690
Size: 2945 6338
ScaI AGT'ACT
Cuts at: 2216 7531 9243 2216
Size: 5315 1712 2256
Fragments arranged by size:
5315 2256 1712
SexAI A'CCwGG_T
Cuts at: 2295 3930 2295
Size: 1635 7648
SfcI C'TryA_G
Cuts at: 826 1045 2147 2653 2874 3038 3052 3686 3715
Size: 219 1102 506 221 164 14 634 29
Cuts at: 3715 5070 5531 5903 6631 7766 8447 8638 826
Size: 1355 461 372 728 1135 681 191 1471
Fragments arranged by size:
1471 1355 1135 1102 728 681 634 506
461 372 221 219 191 164 29 14
SfoI GGC'GCC
Cuts at: 2508 6440 2508
Size: 3932 5351
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9283
SmaI CCC'GGG
Cuts at: 1872 2746 3212 4340 4787 6610 1872
Size: 874 466 1128 447 1823 4545
Fragments arranged by size:
4545 1823 1128 874 466 447
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3227 3947 7387 8288 8550 820
Size: 197 926 1284 720 3440 901 262 1553
Fragments arranged by size:
3440 1553 1284 926 901 720 262 197
SnaBI TAC'GTA
Cuts at: 493 6580 7074 493
Size: 6087 494 2702
Fragments arranged by size:
6087 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9283
SphI G_CATG'C
Cuts at: 1132 3649 6641 1132
Size: 2517 2992 3774
Fragments arranged by size:
3774 2992 2517
SspI AAT'ATT
Cuts at: 5 52 7207 5
Size: 47 7155 2081
Fragments arranged by size:
7155 2081 47
StuI AGG'CCT
Cuts at: 1517 3020 3953 4874 1517
Size: 1503 933 921 5926
Fragments arranged by size:
5926 1503 933 921
StyI C'CwwG_G
Cuts at: 513 1065 3260 3541 3633 4107 4291 4584 5271
Size: 552 2195 281 92 474 184 293 687
Cuts at: 5271 5736 6722 513
Size: 465 986 3074
Fragments arranged by size:
3074 2195 986 687 552 474 465 293
281 184 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4063 4609 4849 6124 7310 7327 7480
Size: 1255 2483 546 240 1275 1186 17 153
Cuts at: 7480 7665 8110 325
Size: 185 445 1498
Fragments arranged by size:
2483 1498 1275 1255 1186 546 445 240
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 4276 4567
Size: 274 80 33 51 1443 237 2062 291
Cuts at: 4567 7529 9241 96
Size: 2962 1712 138
Fragments arranged by size:
2962 2062 1712 1443 291 274 237 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2683 2711 2715 2743 3068 3100 3360
Size: 32 414 28 4 28 325 32 260
Cuts at: 3360 3392 6332 6364 9243 9275 2237
Size: 32 2940 32 2879 32 2245
Fragments arranged by size:
2940 2879 2245 414 325 260 32 32
32 32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9283
XbaI T'CTAG_A
Cuts at: 6619 6619
Size: 9283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2576 3267 4321 5269 6074 2576
Size: 691 1054 948 805 5785
Fragments arranged by size:
5785 1054 948 805 691
XhoI C'TCGA_G
Cuts at: 1943 3227 1943
Size: 1284 7999
XmaI C'CCGG_G
Cuts at: 1870 2744 3210 4338 4785 6608 1870
Size: 874 466 1128 447 1823 4545
Fragments arranged by size:
4545 1823 1128 874 466 447
XmnI GAAnn'nnTTC
Cuts at: 7412 7412
Size: 9283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7091 276
Size: 53 83 186 936 5557 2468
Fragments arranged by size:
5557 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI
FseI NruI NsiI PacI PsrI RsrII SacII SfiI
SrfI SwaI