Restriction map for
pFN21ASDB1480
(Circular) (Six-base) MAPSORT of: pFN21ASDB1480.seq Check: 449 from: 1 to: 7687
pFN21ASDB1480 7687 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3886 3886
Size: 7687
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4854 5497 278
Size: 53 83 186 936 3318 643 2468
Fragments arranged by size:
3318 2468 936 643 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3093 5030 1937
Size: 1156 1937 4594
Fragments arranged by size:
4594 1937 1156
Acc65I G'GTAC_C
Cuts at: 5008 5008
Size: 7687
AclI AA'CG_TT
Cuts at: 1563 5814 6187 1563
Size: 4251 373 3063
Fragments arranged by size:
4251 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3962 5750 7134 7402 1632
Size: 1302 1028 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 1302 1028 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7490
AflIII A'CryG_T
Cuts at: 1203 2211 6586 7563 1203
Size: 1008 4375 977 1327
Fragments arranged by size:
4375 1327 1008 977
AgeI A'CCGG_T
Cuts at: 6518 6518
Size: 7687
AhdI GACnn_n'nnGTC
Cuts at: 4855 6416 4855
Size: 1561 6126
AleI CACnn'nnGTG
Cuts at: 1166 2507 4103 1166
Size: 1341 1596 4750
Fragments arranged by size:
4750 1596 1341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4742 1184
Size: 3558 4129
AlwNI CAG_nnn'CTG
Cuts at: 2111 5430 7002 2111
Size: 3319 1572 2796
Fragments arranged by size:
3319 2796 1572
ApaLI G'TGCA_C
Cuts at: 5744 6900 5744
Size: 1156 6531
ApoI r'AATT_y
Cuts at: 1493 4996 5222 5374 7483 1493
Size: 3503 226 152 2109 1697
Fragments arranged by size:
3503 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6241 160
Size: 872 5209 1606
Fragments arranged by size:
5209 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3121 4576 5012 1870
Size: 73 745 433 1455 436 4545
Fragments arranged by size:
4545 1455 745 436 433 73
BamHI G'GATC_C
Cuts at: 5017 5384 5017
Size: 367 7320
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2267 2633 2767 2819
Size: 325 217 627 472 8 366 134 52
Cuts at: 2819 3131 3524 4702 5008 6463 618
Size: 312 393 1178 306 1455 1842
Fragments arranged by size:
1842 1455 1178 627 472 393 366 325
312 306 217 134 52 8
BanII G_rGCy'C
Cuts at: 1391 3278 3456 4323 5006 1391
Size: 1887 178 867 683 4072
Fragments arranged by size:
4072 1887 867 683 178
BbeI G_GCGC'C
Cuts at: 2263 2271 2637 3528 4706 2263
Size: 8 366 891 1178 5244
Fragments arranged by size:
5244 1178 891 366 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 3529 3673 928
Size: 971 691 939 144 4942
Fragments arranged by size:
4942 971 939 691 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2683 2717 3312 3346 4261 4295 4532
Size: 34 746 34 595 34 915 34 237
Cuts at: 4532 4566 4567 4601 5878 5912 1903
Size: 34 1 34 1277 34 3678
Fragments arranged by size:
3678 1277 915 746 595 237 34 34
34 34 34 34 34 1
BciVI GTATCCnnnnn_n'
Cuts at: 2431 4943 5579 6789 2431
Size: 2512 636 1210 3329
Fragments arranged by size:
3329 2512 1210 636
BclI T'GATC_A
Cuts at: 1548 4110 1548
Size: 2562 5125
BfrBI ATG'CAT
Cuts at: 3349 3349
Size: 7687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2776 2943 3180
Size: 107 122 71 631 605 1104 167 237
Cuts at: 3180 6298 136
Size: 3118 1525
Fragments arranged by size:
3118 1525 1104 631 605 237 167 122
107 71
BglII A'GATC_T
Cuts at: 3804 3963 7682 3804
Size: 159 3719 3809
Fragments arranged by size:
3809 3719 159
BlpI GC'TnA_GC
Cuts at: 2115 5104 2115
Size: 2989 4698
Bme1580I G_kGCm'C
Cuts at: 1790 2615 2772 3553 4372 5748 6904 1790
Size: 825 157 781 819 1376 1156 2573
Fragments arranged by size:
2573 1376 1156 825 819 781 157
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3223 4513 5418
Size: 416 53 83 399 24 1806 1290 905
Cuts at: 5418 6376 442
Size: 958 1753
Fragments arranged by size:
1806 1753 1290 958 905 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 3104 4153 2479
Size: 625 1049 6013
Fragments arranged by size:
6013 1049 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3068 4097 4808 6347 1239
Size: 1829 1029 711 1539 2579
Fragments arranged by size:
2579 1829 1539 1029 711
Bpu10I CC'TnA_GC
Cuts at: 4025 4025
Size: 7687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3971 5812 6677 6975 3971
Size: 1841 865 298 4683
Fragments arranged by size:
4683 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 3026 6350 882
Size: 617 843 684 3324 2219
Fragments arranged by size:
3324 2219 843 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 2196 2284 4984 5478 493
Size: 711 992 88 2700 494 2702
Fragments arranged by size:
2702 2700 992 711 494 88
BsaBI GATnn'nnATC
Cuts at: 3809 4112 4388 7681 3809
Size: 303 276 3293 3815
Fragments arranged by size:
3815 3293 303 276
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2260 2268 2634 3525
Size: 53 83 186 936 727 8 366 891
Cuts at: 3525 4703 4851 5494 5876 6511 275
Size: 1178 148 643 382 635 1451
Fragments arranged by size:
1451 1178 936 891 727 643 635 382
366 186 148 83 53 8
BsaWI w'CCGG_w
Cuts at: 3071 4422 6120 6518 6792 6939 7350 3071
Size: 1351 1698 398 274 147 411 3408
Fragments arranged by size:
3408 1698 1351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 4466 4496 1178
Size: 30 165 30 876 30 2157 30 4369
Fragments arranged by size:
4369 2157 876 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3652 4702 1186
Size: 2466 1050 4171
Fragments arranged by size:
4171 2466 1050
BseYI C'CCAG_C
Cuts at: 2038 2119 2324 3393 6890 7386 2038
Size: 81 205 1069 3497 496 2339
Fragments arranged by size:
3497 2339 1069 496 205 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2968 3121 3527 4571 1163
Size: 573 1232 153 406 1044 4279
Fragments arranged by size:
4279 1232 1044 573 406 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3669 5137 5898 6926 1540
Size: 275 186 1668 1468 761 1028 2301
Fragments arranged by size:
2301 1668 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2672 3456 4100 5748 5833 6904 2672
Size: 784 644 1648 85 1071 3455
Fragments arranged by size:
3455 1648 1071 784 644 85
BsmI GAATG_Cn'
Cuts at: 5198 5291 5198
Size: 93 7594
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2615 2672 2772 3278 3456 3553 4100
Size: 399 825 57 100 506 178 97 547
Cuts at: 4100 4323 4372 5006 5748 5833 6904 1391
Size: 223 49 634 742 85 1071 2174
Fragments arranged by size:
2174 1071 825 742 634 547 506 399
223 178 100 97 85 57 49
BspEI T'CCGG_A
Cuts at: 3071 7350 3071
Size: 4279 3408
BspHI T'CATG_A
Cuts at: 2490 5576 7306 2490
Size: 3086 1730 2871
Fragments arranged by size:
3086 2871 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 3886 4734 5042 844
Size: 2378 664 848 308 3489
Fragments arranged by size:
3489 2378 848 664 308
BsrBI CCG'CTC
Cuts at: 1394 1823 4495 5574 1394
Size: 429 2672 1079 3507
Fragments arranged by size:
3507 2672 1079 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 2947 3687 6182 6356 65
Size: 1183 1194 505 740 2495 174 1396
Fragments arranged by size:
2495 1396 1194 1183 740 505 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3521 4629 4832 6331 6518 1700
Size: 110 1711 1108 203 1499 187 2869
Fragments arranged by size:
2869 1711 1499 1108 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 3157 96
Size: 3061 4626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7687
BssSI C'ACGA_G
Cuts at: 5747 6759 5747
Size: 1012 6675
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2826 3144 794
Size: 2032 318 5337
Fragments arranged by size:
5337 2032 318
BstBI TT'CG_AA
Cuts at: 2007 6491 2007
Size: 4484 3203
BstEII G'GTnAC_C
Cuts at: 1184 3499 1184
Size: 2315 5372
BstXI CCAn_nnnn'nTGG
Cuts at: 2148 2413 2564 3410 4372 4801 2148
Size: 265 151 846 962 429 5034
Fragments arranged by size:
5034 962 846 429 265 151
BstYI r'GATC_y
Cuts at: 1972 3243 3804 3963 4383 5017 5384 5769 5786
Size: 1271 561 159 420 634 367 385 17
Cuts at: 5786 7238 7324 7336 7682 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1271 634 561 420 385 367
346 159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2076 2263 2423 3366 3558 4566
Size: 552 957 54 187 160 943 192 1008
Cuts at: 4566 4698 513
Size: 132 3502
Fragments arranged by size:
3502 1008 957 943 552 192 187 160
132 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2569 2658 2686 3688 4195 487
Size: 683 56 1343 89 28 1002 507 3979
Fragments arranged by size:
3979 1343 1002 683 507 89 56 28
BtsI GCAGTG_nn'
Cuts at: 3342 3852 3937 5203 5997 6017 3342
Size: 510 85 1266 794 20 5012
Fragments arranged by size:
5012 1266 794 510 85 20
ClaI AT'CG_AT
Cuts at: 1554 3813 1554
Size: 2259 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3097 3132 529
Size: 35 2533 35 5084
Fragments arranged by size:
5084 2533 35 35
DraI TTT'AAA
Cuts at: 4991 4991
Size: 7687
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3988 6694 809
Size: 507 2672 2706 1802
Fragments arranged by size:
2706 2672 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2482 3666
Size: 54 1425 50 275 18 558 94 1184
Cuts at: 3666 3781 3934 3972 4437 4642 4906 5134 6023
Size: 115 153 38 465 205 264 228 889
Cuts at: 6023 7652 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1184 889 558 465 275 264
228 205 153 115 94 54 50 43
38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3666 5134 1537
Size: 275 1854 1468 4090
Fragments arranged by size:
4090 1854 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2709 5617 1353
Size: 795 561 2908 3423
Fragments arranged by size:
3423 2908 795 561
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4392 6274 6648 6794 387
Size: 1064 138 2803 1882 374 146 1280
Fragments arranged by size:
2803 1882 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3454 3454
Size: 7687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2934 3068 3962 4097 4808 5750 6347
Size: 393 1302 134 894 135 711 942 597
Cuts at: 6347 7134 7402 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1302 942 894 787 711 597 393
268 135 134
EcoNI CCTnn'n_nnAGG
Cuts at: 2142 2142
Size: 7687
EcoO109I rG'GnC_Cy
Cuts at: 3085 3511 3832 3085
Size: 426 321 6940
Fragments arranged by size:
6940 426 321
EcoRI G'AATT_C
Cuts at: 4996 5374 4996
Size: 378 7309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 3610 4837 2368
Size: 1242 1227 5218
Fragments arranged by size:
5218 1242 1227
FspI TGC'GCA
Cuts at: 6193 6193
Size: 7687
HaeII r_GCGC'y
Cuts at: 1678 2263 2271 2637 2782 3528 3577 4015 4584
Size: 585 8 366 145 746 49 438 569
Cuts at: 4584 4706 6834 1678
Size: 122 2128 2531
Fragments arranged by size:
2531 2128 746 585 569 438 366 145
122 49 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 2369 2401 2404
Size: 32 135 32 547 32 218 32 3
Cuts at: 2404 2436 2657 2689 4331 4363 4466 4498 4663
Size: 32 221 32 1642 32 103 32 165
Cuts at: 4663 4695 4841 4873 6328 6360 6402 6434 1373
Size: 32 146 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1642 1455 547 221 218 165 146
135 103 42 32 32 32 32 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4285 4654 5031 5277 669
Size: 988 281 2347 369 377 246 3079
Fragments arranged by size:
3079 2347 988 377 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7687
HpaI GTT'AAC
Cuts at: 5277 5277
Size: 7687
KasI G'GCGC_C
Cuts at: 2259 2267 2633 3524 4702 2259
Size: 8 366 891 1178 5244
Fragments arranged by size:
5244 1178 891 366 8
KpnI G_GTAC'C
Cuts at: 5012 5012
Size: 7687
MfeI C'AATT_G
Cuts at: 5286 5286
Size: 7687
MluI A'CGCG_T
Cuts at: 7563 7563
Size: 7687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4290 6801 6985 1305
Size: 2985 2511 184 2007
Fragments arranged by size:
2985 2511 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3783 3936 3974 4908 10
Size: 54 1425 343 1951 153 38 934 2789
Fragments arranged by size:
2789 1951 1425 934 343 153 54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2146 2282 2507 2588 3692 4103 4466
Size: 648 980 136 225 81 1104 411 363
Cuts at: 4466 4799 5645 6004 6163 518
Size: 333 846 359 159 2042
Fragments arranged by size:
2042 1104 980 846 648 411 363 359
333 225 159 136 81
MspA1I CmG'CkG
Cuts at: 1606 2065 2538 2697 3144 3582 3671 4189 4217
Size: 459 473 159 447 438 89 518 28
Cuts at: 4217 4395 4732 5103 5780 6928 7173 1606
Size: 178 337 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 518 473 459 447 438
371 337 245 178 159 89 28
NaeI GCC'GGC
Cuts at: 3523 4834 3523
Size: 1311 6376
NarI GG'CG_CC
Cuts at: 2260 2268 2634 3525 4703 2260
Size: 8 366 891 1178 5244
Fragments arranged by size:
5244 1178 891 366 8
NcoI C'CATG_G
Cuts at: 513 1065 2022 2423 4566 4698 513
Size: 552 957 401 2143 132 3502
Fragments arranged by size:
3502 2143 957 552 401 132
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7687
NgoMIV G'CCGG_C
Cuts at: 3521 4832 3521
Size: 1311 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7687
NotI GC'GGCC_GC
Cuts at: 5134 5134
Size: 7687
NsiI A_TGCA'T
Cuts at: 3351 3351
Size: 7687
NspI r_CATG'y
Cuts at: 1132 1229 2578 2788 2980 4201 5045 6590 1132
Size: 97 1349 210 192 1221 844 1545 2229
Fragments arranged by size:
2229 1545 1349 1221 844 210 192 97
PasI CC'CwG_GG
Cuts at: 1348 4372 1348
Size: 3024 4663
PciI A'CATG_T
Cuts at: 6586 6586
Size: 7687
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4192 4395 1108
Size: 3084 203 4400
Fragments arranged by size:
4400 3084 203
PfoI T'CCnGG_A
Cuts at: 2556 2643 3420 3633 2556
Size: 87 777 213 6610
Fragments arranged by size:
6610 777 213 87
PmeI GTTT'AAAC
Cuts at: 4991 4991
Size: 7687
PmlI CAC'GTG
Cuts at: 2284 2284
Size: 7687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 5756 5788 7269 7301 2011
Size: 32 3713 32 1481 32 2397
Fragments arranged by size:
3713 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3085 3832 3085
Size: 747 6940
PshAI GACnn'nnGTC
Cuts at: 1613 2527 1613
Size: 914 6773
PsiI TTA'TAA
Cuts at: 5257 5257
Size: 7687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 7655
PstI C_TGCA'G
Cuts at: 830 2315 3438 3932 5039 830
Size: 1485 1123 494 1107 3478
Fragments arranged by size:
3478 1485 1123 1107 494
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7687
PvuII CAG'CTG
Cuts at: 2697 4189 4217 2697
Size: 1492 28 6167
Fragments arranged by size:
6167 1492 28
SacI G_AGCT'C
Cuts at: 3456 3456
Size: 7687
SalI G'TCGA_C
Cuts at: 1936 5029 1936
Size: 3093 4594
SbfI CC_TGCA'GG
Cuts at: 5039 5039
Size: 7687
ScaI AGT'ACT
Cuts at: 3440 5935 7647 3440
Size: 2495 1712 3480
Fragments arranged by size:
3480 2495 1712
SexAI A'CCwGG_T
Cuts at: 2199 4293 2199
Size: 2094 5593
SfcI C'TryA_G
Cuts at: 826 1045 2103 2311 2950 3434 3577 3928 5035
Size: 219 1058 208 639 484 143 351 1107
Cuts at: 5035 6170 6851 7042 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 1058 681 639 484 351
219 208 191 143
SfoI GGC'GCC
Cuts at: 2261 2269 2635 3526 4704 2261
Size: 8 366 891 1178 5244
Fragments arranged by size:
5244 1178 891 366 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7687
SmaI CCC'GGG
Cuts at: 1872 2690 5014 1872
Size: 818 2324 4545
Fragments arranged by size:
4545 2324 818
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3986 5791 6692 6954 820
Size: 197 926 2043 1805 901 262 1553
Fragments arranged by size:
2043 1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4984 5478 493
Size: 4491 494 2702
Fragments arranged by size:
4491 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7687
SphI G_CATG'C
Cuts at: 1132 4201 5045 1132
Size: 3069 844 3774
Fragments arranged by size:
3774 3069 844
SspI AAT'ATT
Cuts at: 5 52 5611 5
Size: 47 5559 2081
Fragments arranged by size:
5559 2081 47
StuI AGG'CCT
Cuts at: 1517 2876 3183 1517
Size: 1359 307 6021
Fragments arranged by size:
6021 1359 307
StyI C'CwwG_G
Cuts at: 513 1065 2022 2423 2863 3861 4566 4698 5126
Size: 552 957 401 440 998 705 132 428
Cuts at: 5126 513
Size: 3074
Fragments arranged by size:
3074 998 957 705 552 440 428 401
132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2416 2468 3203 3242 5714 5731 5884
Size: 1255 836 52 735 39 2472 17 153
Cuts at: 5884 6069 6514 325
Size: 185 445 1498
Fragments arranged by size:
2472 1498 1255 836 735 445 185 153
52 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3157 3231 3438
Size: 274 80 33 51 1443 1180 74 207
Cuts at: 3438 3462 3591 3750 4809 5933 7645 96
Size: 24 129 159 1059 1124 1712 138
Fragments arranged by size:
1712 1443 1180 1124 1059 274 207 159
138 129 80 74 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 7647 7679 2744
Size: 32 4871 32 2752
Fragments arranged by size:
4871 2752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3089 1316
Size: 1773 5914
XbaI T'CTAG_A
Cuts at: 4333 5023 4333
Size: 690 6997
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7687
XmaI C'CCGG_G
Cuts at: 1870 2688 5012 1870
Size: 818 2324 4545
Fragments arranged by size:
4545 2324 818
XmnI GAAnn'nnTTC
Cuts at: 5816 5816
Size: 7687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4852 5495 276
Size: 53 83 186 936 3318 643 2468
Fragments arranged by size:
3318 2468 936 643 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI
PacI PspOMI PspXI RsrII SacII SanDI SapI SfiI
SrfI SwaI XcmI