Restriction map for
pFN21ASDB1453
(Circular) (Six-base) MAPSORT of: pFN21ASDB1453.seq Check: 8292 from: 1 to: 6124
pFN21ASDB1453 6124 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2851 3934 278
Size: 53 83 186 936 1315 1083 2468
Fragments arranged by size:
2468 1315 1083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4187 5571 5839 1632
Size: 2555 1384 268 1917
Fragments arranged by size:
2555 1917 1384 268
AfeI AGC'GCT
Cuts at: 3362 3362
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 3278 820
Size: 197 2261 3666
Fragments arranged by size:
3666 2261 197
AflIII A'CryG_T
Cuts at: 1203 2359 3351 5023 6000 1203
Size: 1156 992 1672 977 1327
Fragments arranged by size:
1672 1327 1156 992 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 2424 1166
Size: 1258 4866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 6092
AlwNI CAG_nnn'CTG
Cuts at: 3269 3867 5439 3269
Size: 598 1572 3954
Fragments arranged by size:
3954 1572 598
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2107 2753 2809 3433 3659 3811 5920 1493
Size: 614 646 56 624 226 152 2109 1697
Fragments arranged by size:
2109 1697 646 624 614 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3445 4900 618
Size: 325 217 627 1658 1455 1842
Fragments arranged by size:
1842 1658 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3133 3443 1391
Size: 1742 310 4072
Fragments arranged by size:
4072 1742 310
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3331 928
Size: 971 1432 3721
Fragments arranged by size:
3721 1432 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2107 2141 4315 4349 1903
Size: 34 170 34 2174 34 3678
Fragments arranged by size:
3678 2174 170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 2952 1548
Size: 1404 4720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 4185 5341 1790
Size: 2395 1156 2573
Fragments arranged by size:
2573 2395 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2778 3190 3343
Size: 416 53 83 399 24 1361 412 153
Cuts at: 3343 3855 4813 442
Size: 512 958 1753
Fragments arranged by size:
1753 1361 958 512 416 412 399 153
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2858 1056
Size: 1802 4322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2553 3039 3156 4784 1239
Size: 884 430 486 117 1628 2579
Fragments arranged by size:
2579 1628 884 486 430 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2988 4249 5114 5412 2988
Size: 1261 865 298 3700
Fragments arranged by size:
3700 1261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2990 3193 4787 882
Size: 617 1491 203 1594 2219
Fragments arranged by size:
2219 1594 1491 617 203
BsaAI yAC'GTr
Cuts at: 493 1204 3354 3421 3915 493
Size: 711 2150 67 494 2702
Fragments arranged by size:
2702 2150 711 494 67
BsaBI GATnn'nnATC
Cuts at: 2969 6118 2969
Size: 3149 2975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2848 3931 4313 4948
Size: 53 83 186 936 1315 1083 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1451 1315 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2214 4557 4955 5229 5376 5787 2214
Size: 2343 398 274 147 411 2551
Fragments arranged by size:
2551 2343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 3037 3067 1178
Size: 30 165 30 1173 30 431 30 4235
Fragments arranged by size:
4235 1173 431 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2328 2597 1186
Size: 1030 112 269 4713
Fragments arranged by size:
4713 1030 269 112
BseYI C'CCAG_C
Cuts at: 5327 5823 5327
Size: 496 5628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3133 4185 4270 5341 3133
Size: 1052 85 1071 3916
Fragments arranged by size:
3916 1071 1052 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3133 3443 4185 4270 5341 1391
Size: 399 1343 310 742 85 1071 2174
Fragments arranged by size:
2174 1343 1071 742 399 310 85
BspEI T'CCGG_A
Cuts at: 2214 5787 2214
Size: 3573 2551
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2401 3211 3479 844
Size: 1557 810 268 3489
Fragments arranged by size:
3489 1557 810 268
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2034 2376 2886 4619 4793 65
Size: 1183 786 342 510 1733 174 1396
Fragments arranged by size:
1733 1396 1183 786 510 342 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 2007 4928 2007
Size: 2921 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 3085 3454 3821 4206 4223 5675 5761 5773
Size: 1113 369 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1113 385 369 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4615
Fragments arranged by size:
4615 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4434 4454 3640
Size: 794 20 5310
Fragments arranged by size:
5310 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 3291 3571
Size: 54 1425 50 275 18 586 875 280
Cuts at: 3571 4460 6089 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 875 586 280 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 2574 4054 1353
Size: 902 319 1480 3423
Fragments arranged by size:
3423 1480 902 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3131 3131
Size: 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2553 3039 3156 4187 4784 5571
Size: 393 491 430 486 117 1031 597 787
Cuts at: 5571 5839 1239
Size: 268 1524
Fragments arranged by size:
1524 1031 787 597 491 486 430 393
268 117
EcoO109I rG'GnC_Cy
Cuts at: 3370 3370
Size: 6124
EcoRI G'AATT_C
Cuts at: 2809 3433 3811 2809
Size: 624 378 5122
Fragments arranged by size:
5122 624 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3177 3177
Size: 6124
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 3364 5271 1678
Size: 1686 1907 2531
Fragments arranged by size:
2531 1907 1686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2924 2956 3037
Size: 32 135 32 704 32 616 32 81
Cuts at: 3037 3069 3227 3259 4765 4797 4839 4871 1373
Size: 32 158 32 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 704 616 158 135 81 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3468 3714 669
Size: 988 281 1240 290 246 3079
Fragments arranged by size:
3079 1240 988 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2418 3293 10
Size: 54 1425 343 586 875 2841
Fragments arranged by size:
2841 1425 875 586 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2364 2424 2444 4082 4441 4600
Size: 648 861 337 60 20 1638 359 159
Cuts at: 4600 518
Size: 2042
Fragments arranged by size:
2042 1638 861 648 359 337 159 60
20
MspA1I CmG'CkG
Cuts at: 1606 3540 4217 5365 5610 1606
Size: 1934 677 1148 245 2120
Fragments arranged by size:
2120 1934 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4615
Fragments arranged by size:
4615 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 2854 1052
Size: 1802 4322
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2231 2363 3482 5027 1132
Size: 97 1002 132 1119 1545 2229
Fragments arranged by size:
2229 1545 1119 1002 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 2359 5023 2359
Size: 2664 3460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 3133 3133
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2715 1613
Size: 1102 5022
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2322 2354 2322
Size: 32 6092
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SacI G_AGCT'C
Cuts at: 3133 3133
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 2482 3472 4607 5288 5479 826
Size: 219 1437 990 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 990 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3003 3278 4228 5129 5391 820
Size: 197 926 1060 275 950 901 262 1553
Fragments arranged by size:
1553 1060 950 926 901 275 262 197
SnaBI TAC'GTA
Cuts at: 493 3421 3915 493
Size: 2928 494 2702
Fragments arranged by size:
2928 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2190 1517
Size: 673 5451
StyI C'CwwG_G
Cuts at: 513 1065 2022 3081 3563 513
Size: 552 957 1059 482 3074
Fragments arranged by size:
3074 1059 957 552 482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4151 4168 4321 4506 4951 325
Size: 1255 2571 17 153 185 445 1498
Fragments arranged by size:
2571 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4370 6082 96
Size: 274 80 33 51 1443 2393 1712 138
Fragments arranged by size:
2393 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6084 6116 6084
Size: 32 6092
Tth111I GACn'n_nGTC
Cuts at: 1316 3335 1316
Size: 2019 4105
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2098
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 2477 4253 2477
Size: 1776 4348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2849 3932 276
Size: 53 83 186 936 1315 1083 2468
Fragments arranged by size:
2468 1315 1083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI