Restriction map for
pFN21ASDB1316
(Circular) (Six-base) MAPSORT of: pFN21ASDB1316.seq Check: 7421 from: 1 to: 7315
pFN21ASDB1316 7315 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3554 3554
Size: 7315
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2555 4566 5125 278
Size: 53 83 186 936 1019 2011 559 2468
Fragments arranged by size:
2468 2011 1019 936 559 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 4658 1937
Size: 778 1943 4594
Fragments arranged by size:
4594 1943 778
Acc65I G'GTAC_C
Cuts at: 3106 4636 3106
Size: 1530 5785
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2954 4409 4598 5378 6762 7030 1632
Size: 1322 1455 189 780 1384 268 1917
Fragments arranged by size:
1917 1455 1384 1322 780 268 189
AflII C'TTAA_G
Cuts at: 820 1017 4205 820
Size: 197 3188 3930
Fragments arranged by size:
3930 3188 197
AflIII A'CryG_T
Cuts at: 1203 2394 6214 7191 1203
Size: 1191 3820 977 1327
Fragments arranged by size:
3820 1327 1191 977
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 6044 6044
Size: 7315
AleI CACnn'nnGTG
Cuts at: 1166 2732 3062 4034 1166
Size: 1566 330 972 4447
Fragments arranged by size:
4447 1566 972 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2806 1184
Size: 1622 5693
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2429 2461 2429
Size: 32 7283
AlwNI CAG_nnn'CTG
Cuts at: 3313 3448 3895 4387 5058 6630 3313
Size: 135 447 492 671 1572 3998
Fragments arranged by size:
3998 1572 671 492 447 135
ApaLI G'TGCA_C
Cuts at: 2032 2132 2998 5372 6528 2032
Size: 100 866 2374 1156 2819
Fragments arranged by size:
2819 2374 1156 866 100
ApoI r'AATT_y
Cuts at: 1493 2593 3175 4319 4624 4850 5002 7111 1493
Size: 1100 582 1144 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 1100 582 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 2926 3383 3499 3771 4640 1870
Size: 73 277 706 457 116 272 869 4545
Fragments arranged by size:
4545 869 706 457 277 272 116 73
BamHI G'GATC_C
Cuts at: 4645 5012 4645
Size: 367 6948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2938 3106 3542 4636
Size: 325 217 627 429 722 168 436 1094
Cuts at: 4636 6091 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1094 722 627 436 429 325
217 168
BanII G_rGCy'C
Cuts at: 1391 3364 3593 3635 3818 4634 1391
Size: 1973 229 42 183 816 4072
Fragments arranged by size:
4072 1973 816 229 183 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2301 2881 2902 3022 3450 3919
Size: 971 325 77 580 21 120 428 469
Cuts at: 3919 4053 928
Size: 134 4190
Fragments arranged by size:
4190 971 580 469 428 325 134 120
77 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2844 2878 4523 4557 5506 5540 1903
Size: 34 907 34 1645 34 949 34 3678
Fragments arranged by size:
3678 1645 949 907 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4289 5207 6417 4289
Size: 918 1210 5187
Fragments arranged by size:
5187 1210 918
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2225 2697 3521
Size: 107 122 71 631 605 553 472 824
Cuts at: 3521 4085 5926 136
Size: 564 1841 1525
Fragments arranged by size:
1841 1525 824 631 605 564 553 472
122 107 71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 2186 4732 2186
Size: 2546 4769
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2136 2221 3002 3683 5376 6532 1790
Size: 246 100 85 781 681 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 781 681 246 100 85
BmgBI CAC'GTC
Cuts at: 1318 2668 3949 1318
Size: 1350 1281 4684
Fragments arranged by size:
4684 1350 1281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5046 6004 442
Size: 416 53 83 399 24 3629 958 1753
Fragments arranged by size:
3629 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3144 3607 5975 1239
Size: 1905 463 2368 2579
Fragments arranged by size:
2579 2368 1905 463
Bpu10I CC'TnA_GC
Cuts at: 2918 2918
Size: 7315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5440 6305 6603 5440
Size: 865 298 6152
Fragments arranged by size:
6152 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2293 3404 4463 5978 882
Size: 617 794 1111 1059 1515 2219
Fragments arranged by size:
2219 1515 1111 1059 794 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 4612 5106 493
Size: 711 1191 2217 494 2702
Fragments arranged by size:
2702 2217 1191 711 494
BsaBI GATnn'nnATC
Cuts at: 4160 7309 4160
Size: 3149 4166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2552 4563 5122 5504
Size: 53 83 186 936 1019 2011 559 382
Cuts at: 5504 6139 275
Size: 635 1451
Fragments arranged by size:
2011 1451 1019 936 635 559 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3300 5748 6146 6420 6567 6978 3300
Size: 2448 398 274 147 411 3637
Fragments arranged by size:
3637 2448 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 3152 3182 4099
Size: 30 165 30 1332 30 387 30 917
Cuts at: 4099 4129 4596 4626 1178
Size: 30 467 30 3867
Fragments arranged by size:
3867 1332 917 467 387 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3038 3422 3917 4084 4217 1186
Size: 1852 384 495 167 133 4284
Fragments arranged by size:
4284 1852 495 384 167 133
BseYI C'CCAG_C
Cuts at: 2500 3414 3635 4075 4176 4422 6518 7014 2500
Size: 914 221 440 101 246 2096 496 2801
Fragments arranged by size:
2801 2096 914 496 440 246 221 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2334 3350 3602 4103 1163
Size: 573 426 172 1016 252 501 4375
Fragments arranged by size:
4375 1016 573 501 426 252 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4765 5526 6554 1540
Size: 275 186 2764 761 1028 2301
Fragments arranged by size:
2764 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2124 2136 2919 3002 3059 3364 3390 4440
Size: 88 12 783 83 57 305 26 1050
Cuts at: 4440 5376 5461 6532 2036
Size: 936 85 1071 2819
Fragments arranged by size:
2819 1071 1050 936 783 305 88 85
83 57 26 12
BsmI GAATG_Cn'
Cuts at: 2411 4826 4919 2411
Size: 2415 93 4807
Fragments arranged by size:
4807 2415 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4388 4388
Size: 7315
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2124 2136 2221 2919 3002 3059
Size: 399 246 88 12 85 698 83 57
Cuts at: 3059 3364 3390 3593 3635 3683 3818 4440 4634
Size: 305 26 203 42 48 135 622 194
Cuts at: 4634 5376 5461 6532 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 698 622 399 305 246
203 194 135 88 85 85 83 57
48 42 26 12
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 2149 5204 6934 2149
Size: 3055 1730 2530
Fragments arranged by size:
3055 2530 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 3614 4308 4670 844
Size: 2710 60 694 362 3489
Fragments arranged by size:
3489 2710 694 362 60
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 4326 5202 1394
Size: 429 1840 663 876 3507
Fragments arranged by size:
3507 1840 876 663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5810 5984 65
Size: 1183 4562 174 1396
Fragments arranged by size:
4562 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2419 3705 4133 4460 5959 6146 1700
Size: 110 609 1286 428 327 1499 187 2869
Fragments arranged by size:
2869 1499 1286 609 428 327 187 110
BsrGI T'GTAC_A
Cuts at: 96 2208 96
Size: 2112 5203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 5375 6387 5375
Size: 1012 6303
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2312 4143 794
Size: 1346 172 1831 3966
Fragments arranged by size:
3966 1831 1346 172
BstBI TT'CG_AA
Cuts at: 2007 6119 2007
Size: 4112 3203
BstEII G'GTnAC_C
Cuts at: 1184 3232 1184
Size: 2048 5267
BstXI CCAn_nnnn'nTGG
Cuts at: 4039 4430 4039
Size: 391 6924
BstYI r'GATC_y
Cuts at: 1972 4645 5012 5397 5414 6866 6952 6964 7310
Size: 2673 367 385 17 1452 86 12 346
Cuts at: 7310 1972
Size: 1977
Fragments arranged by size:
2673 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2813 2813
Size: 7315
BtgI C'CryG_G
Cuts at: 513 1065 2774 3037 3116 4503 513
Size: 552 1709 263 79 1387 3325
Fragments arranged by size:
3325 1709 1387 552 263 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2099 3681 487
Size: 683 56 873 1582 4121
Fragments arranged by size:
4121 1582 873 683 56
BtsI GCAGTG_nn'
Cuts at: 2073 3000 4831 5625 5645 2073
Size: 927 1831 794 20 3743
Fragments arranged by size:
3743 1831 927 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3709 3744 529
Size: 35 3145 35 4100
Fragments arranged by size:
4100 3145 35 35
DraI TTT'AAA
Cuts at: 4619 4619
Size: 7315
DraIII CAC_nnn'GTG
Cuts at: 2140 3084 2140
Size: 944 6371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2870 4762 5651
Size: 54 1425 50 275 18 1040 1892 889
Cuts at: 5651 7280 8
Size: 1629 43
Fragments arranged by size:
1892 1629 1425 1040 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4762 1537
Size: 275 2950 4090
Fragments arranged by size:
4090 2950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2963 3425 4244 4250 4265 5245 1353
Size: 1610 462 819 6 15 980 3423
Fragments arranged by size:
3423 1610 980 819 462 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5902 6276 6422 387
Size: 1064 138 4313 374 146 1280
Fragments arranged by size:
4313 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3362 3362
Size: 7315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2954 3144 3607 4409 4598 5378 5975
Size: 393 1322 190 463 802 189 780 597
Cuts at: 5975 6762 7030 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1322 802 787 780 597 463 393
268 190 189
EcoNI CCTnn'n_nnAGG
Cuts at: 3899 3899
Size: 7315
EcoO109I rG'GnC_Cy
Cuts at: 2817 2922 3310 3514 3571 2817
Size: 105 388 204 57 6561
Fragments arranged by size:
6561 388 204 105 57
EcoRI G'AATT_C
Cuts at: 4624 5002 4624
Size: 378 6937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 7315
FspI TGC'GCA
Cuts at: 5821 5821
Size: 7315
HaeII r_GCGC'y
Cuts at: 1678 2382 6462 1678
Size: 704 4080 2531
Fragments arranged by size:
4080 2531 704
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3117 3149 5956 5988 6030
Size: 32 135 32 1545 32 2807 32 42
Cuts at: 6030 6062 1373
Size: 32 2626
Fragments arranged by size:
2807 2626 1545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4659 4905 669
Size: 988 281 2721 246 3079
Fragments arranged by size:
3079 2721 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7315
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KpnI G_GTAC'C
Cuts at: 3110 4640 3110
Size: 1530 5785
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3786 6429 6613 1305
Size: 2481 2643 184 2007
Fragments arranged by size:
2643 2481 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5493
Fragments arranged by size:
5493 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2360 2732 3062 4034 5273 5632
Size: 648 861 333 372 330 972 1239 359
Cuts at: 5632 5791 518
Size: 159 2042
Fragments arranged by size:
2042 1239 972 861 648 372 359 333
330 159
MspA1I CmG'CkG
Cuts at: 1606 2880 2971 3118 3520 3737 4731 5408 6556
Size: 1274 91 147 402 217 994 677 1148
Cuts at: 6556 6801 1606
Size: 245 2120
Fragments arranged by size:
2120 1274 1148 994 677 402 245 217
147 91
NaeI GCC'GGC
Cuts at: 2421 2421
Size: 7315
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6763
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7315
NgoMIV G'CCGG_C
Cuts at: 2419 2419
Size: 7315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 3052 4673 6218 1132
Size: 97 1823 1621 1545 2229
Fragments arranged by size:
2229 1823 1621 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7315
PciI A'CATG_T
Cuts at: 6214 6214
Size: 7315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3292 3313 1108
Size: 2184 21 5110
Fragments arranged by size:
5110 2184 21
PfoI T'CCnGG_A
Cuts at: 3423 3951 3423
Size: 528 6787
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 4565 4597 5384 5416 6897 6929 2741
Size: 32 1792 32 787 32 1481 32 3127
Fragments arranged by size:
3127 1792 1481 787 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2922 3571 2817
Size: 105 649 6561
Fragments arranged by size:
6561 649 105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspXI vC'TCGA_Gb
Cuts at: 3383 3383
Size: 7315
PstI C_TGCA'G
Cuts at: 830 2370 3198 4667 830
Size: 1540 828 1469 3478
Fragments arranged by size:
3478 1540 1469 828
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 2971 2971
Size: 7315
SacI G_AGCT'C
Cuts at: 3364 3364
Size: 7315
SacII CC_GC'GG
Cuts at: 3119 3119
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SanDI GG'GwC_CC
Cuts at: 3571 3571
Size: 7315
SapI GCTCTTCn'nnn_
Cuts at: 3425 3425
Size: 7315
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 5563 7275 5563
Size: 1712 5603
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 7315
SfcI C'TryA_G
Cuts at: 826 1045 2366 3194 4663 5798 6479 6670 826
Size: 219 1321 828 1469 1135 681 191 1471
Fragments arranged by size:
1471 1469 1321 1135 828 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3521 3521
Size: 7315
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 2222 2928 3773 4642 1872
Size: 350 706 845 869 4545
Fragments arranged by size:
4545 869 845 706 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3383 4205 5419 6320 6582 820
Size: 197 926 1440 822 1214 901 262 1553
Fragments arranged by size:
1553 1440 1214 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 4612 5106 493
Size: 4119 494 2702
Fragments arranged by size:
4119 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4673 1132
Size: 3541 3774
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7315
StyI C'CwwG_G
Cuts at: 513 1065 2633 4054 4754 513
Size: 552 1568 1421 700 3074
Fragments arranged by size:
3074 1568 1421 700 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2113 3076 3299 3641 4067 4487 5342
Size: 1255 533 963 223 342 426 420 855
Cuts at: 5342 5359 5512 5697 6142 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 963 855 533 445 426 420
342 223 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 3045 5561
Size: 274 80 33 51 1443 231 837 2516
Cuts at: 5561 7273 96
Size: 1712 138
Fragments arranged by size:
2516 1712 1443 837 274 231 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4099 4131 7275 7307 4099
Size: 32 3144 32 4107
Fragments arranged by size:
4107 3144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2711 1316
Size: 1395 5920
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3314 3949 4036 4039 4450 3314
Size: 635 87 3 411 6179
Fragments arranged by size:
6179 635 411 87 3
XhoI C'TCGA_G
Cuts at: 1943 3383 1943
Size: 1440 5875
XmaI C'CCGG_G
Cuts at: 1870 2220 2926 3771 4640 1870
Size: 350 706 845 869 4545
Fragments arranged by size:
4545 869 845 706 350
XmnI GAAnn'nnTTC
Cuts at: 2596 5444 2596
Size: 2848 4467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2553 4564 5123 276
Size: 53 83 186 936 1019 2011 559 2468
Fragments arranged by size:
2468 2011 1019 936 559 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PsrI RsrII SfoI SrfI
SwaI