Restriction map for
pFN21ASDB0234
(Circular) (Six-base) MAPSORT of: pFN21ASDB0234.seq Check: 4988 from: 1 to: 8338
pFN21ASDB0234 8338 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6148 278
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 4166 5681 1937
Size: 274 1955 1515 4594
Fragments arranged by size:
4594 1955 1515 274
Acc65I G'GTAC_C
Cuts at: 2825 4299 5659 2825
Size: 1474 1360 5504
Fragments arranged by size:
5504 1474 1360
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 4978 5250 5310 5597 6401 7785 8053
Size: 742 2604 272 60 287 804 1384 268
Cuts at: 8053 1632
Size: 1917
Fragments arranged by size:
2604 1917 1384 804 742 287 272 268
60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8141
AflIII A'CryG_T
Cuts at: 1203 7237 8214 1203
Size: 6034 977 1327
Fragments arranged by size:
6034 1327 977
AgeI A'CCGG_T
Cuts at: 7169 7169
Size: 8338
AhdI GACnn_n'nnGTC
Cuts at: 7067 7067
Size: 8338
AleI CACnn'nnGTG
Cuts at: 1166 3203 4322 4995 1166
Size: 2037 1119 673 4509
Fragments arranged by size:
4509 2037 1119 673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8338
AlwNI CAG_nnn'CTG
Cuts at: 2528 3458 3650 3824 5466 6081 7653 2528
Size: 930 192 174 1642 615 1572 3213
Fragments arranged by size:
3213 1642 1572 930 615 192 174
ApaI G_GGCC'C
Cuts at: 4787 5042 4787
Size: 255 8083
ApaLI G'TGCA_C
Cuts at: 5305 6395 7551 5305
Size: 1090 1156 6092
Fragments arranged by size:
6092 1156 1090
ApoI r'AATT_y
Cuts at: 1493 2274 3323 5195 5647 5873 6025 8134 1493
Size: 781 1049 1872 452 226 152 2109 1697
Fragments arranged by size:
2109 1872 1697 1049 781 452 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6892 160
Size: 872 5860 1606
Fragments arranged by size:
5860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 3042 5277 5663 1870
Size: 73 1099 2235 386 4545
Fragments arranged by size:
4545 2235 1099 386 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2661 2694 2817 2850 2661
Size: 33 123 33 8149
Fragments arranged by size:
8149 123 33 33
BamHI G'GATC_C
Cuts at: 3883 5668 6035 3883
Size: 1785 367 6186
Fragments arranged by size:
6186 1785 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2321 2825 2897 3128 3314
Size: 325 217 627 534 504 72 231 186
Cuts at: 3314 4081 4299 4487 4580 4789 5659 7114 618
Size: 767 218 188 93 209 870 1455 1842
Fragments arranged by size:
1842 1455 870 767 627 534 504 325
231 218 217 209 188 186 93 72
BanII G_rGCy'C
Cuts at: 1391 4787 5042 5657 1391
Size: 3396 255 615 4072
Fragments arranged by size:
4072 3396 615 255
BbeI G_GCGC'C
Cuts at: 2325 2325
Size: 8338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3516 5481 928
Size: 971 1617 1965 3785
Fragments arranged by size:
3785 1965 1617 971
BbvCI CC'TCA_GC
Cuts at: 2334 2334
Size: 8338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6529 6563 1903
Size: 34 4592 34 3678
Fragments arranged by size:
4592 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2266 2416 6230 7440 2266
Size: 150 3814 1210 3164
Fragments arranged by size:
3814 3164 1210 150
BclI T'GATC_A
Cuts at: 1548 2397 2490 1548
Size: 849 93 7396
Fragments arranged by size:
7396 849 93
BfrBI ATG'CAT
Cuts at: 4544 4544
Size: 8338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6949 136
Size: 107 122 71 631 605 5277 1525
Fragments arranged by size:
5277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8333 8333
Size: 8338
BlpI GC'TnA_GC
Cuts at: 5755 5755
Size: 8338
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2902 3317 4086 4787 5042 5309 5451
Size: 349 763 415 769 701 255 267 142
Cuts at: 5451 6399 7555 1790
Size: 948 1156 2573
Fragments arranged by size:
2573 1156 948 769 763 701 415 349
267 255 142
BmgBI CAC'GTC
Cuts at: 1318 3473 1318
Size: 2155 6183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2277 2958 3357
Size: 416 53 83 399 24 860 681 399
Cuts at: 3357 4034 4789 6069 7027 442
Size: 677 755 1280 958 1753
Fragments arranged by size:
1753 1280 958 860 755 681 677 416
399 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 3586 3846 3915 4772 4869 6998 1239
Size: 1353 994 260 69 857 97 2129 2579
Fragments arranged by size:
2579 2129 1353 994 857 260 97 69
Bpu10I CC'TnA_GC
Cuts at: 2334 2334
Size: 8338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2076 2381 3419 5295 6463 7328 7626 2076
Size: 305 1038 1876 1168 865 298 2788
Fragments arranged by size:
2788 1876 1168 1038 865 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3281 4394 5121 7001 882
Size: 617 1782 1113 727 1880 2219
Fragments arranged by size:
2219 1880 1782 1113 727 617
BsaAI yAC'GTr
Cuts at: 493 1204 4909 5635 6129 493
Size: 711 3705 726 494 2702
Fragments arranged by size:
3705 2702 726 711 494
BsaBI GATnn'nnATC
Cuts at: 2684 4475 8332 2684
Size: 1791 3857 2690
Fragments arranged by size:
3857 2690 1791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 6145 6527 7162
Size: 53 83 186 936 789 3823 382 635
Cuts at: 7162 275
Size: 1451
Fragments arranged by size:
3823 1451 936 789 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2883 4200 5215 6771 7169 7443 7590 8001 2883
Size: 1317 1015 1556 398 274 147 411 3220
Fragments arranged by size:
3220 1556 1317 1015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2330 2360 3014 3044 4391
Size: 30 165 30 927 30 654 30 1347
Cuts at: 4391 4421 1178
Size: 30 5095
Fragments arranged by size:
5095 1347 927 654 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2999 4582 4769 5379 1186
Size: 1165 648 1583 187 610 4145
Fragments arranged by size:
4145 1583 1165 648 610 187
BseYI C'CCAG_C
Cuts at: 2231 7541 8037 2231
Size: 5310 496 2532
Fragments arranged by size:
5310 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5029 1163
Size: 573 3293 4472
Fragments arranged by size:
4472 3293 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5788 6549 7577 1540
Size: 275 186 3787 761 1028 2301
Fragments arranged by size:
3787 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5309 5625 6399 6484 7555 5309
Size: 316 774 85 1071 6092
Fragments arranged by size:
6092 1071 774 316 85
BsmI GAATG_Cn'
Cuts at: 2818 3559 5849 5942 2818
Size: 741 2290 93 5214
Fragments arranged by size:
5214 2290 741 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2902 3317 4086 4787 5042 5309
Size: 399 349 763 415 769 701 255 267
Cuts at: 5309 5451 5625 5657 6399 6484 7555 1391
Size: 142 174 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 769 763 742 701 415 399
349 267 255 174 142 85 32
BspEI T'CCGG_A
Cuts at: 4200 5215 8001 4200
Size: 1015 2786 4537
Fragments arranged by size:
4537 2786 1015
BspHI T'CATG_A
Cuts at: 4467 4711 6227 7957 4467
Size: 244 1516 1730 4848
Fragments arranged by size:
4848 1730 1516 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2728 5693 844
Size: 1884 2965 3489
Fragments arranged by size:
3489 2965 1884
BsrBI CCG'CTC
Cuts at: 1394 1823 6225 1394
Size: 429 4402 3507
Fragments arranged by size:
4402 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6833 7007 65
Size: 1183 5585 174 1396
Fragments arranged by size:
5585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4484 4741 5137 6982 7169 1700
Size: 110 2674 257 396 1845 187 2869
Fragments arranged by size:
2869 2674 1845 396 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 2548 96
Size: 2452 5886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8338
BssSI C'ACGA_G
Cuts at: 6398 7410 6398
Size: 1012 7326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5600 794
Size: 4806 3532
BstBI TT'CG_AA
Cuts at: 2007 7142 2007
Size: 5135 3203
BstEII G'GTnAC_C
Cuts at: 1184 3052 1184
Size: 1868 6470
BstYI r'GATC_y
Cuts at: 1972 2158 2524 3151 3883 5668 6035 6420 6437
Size: 186 366 627 732 1785 367 385 17
Cuts at: 6437 7889 7975 7987 8333 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1785 1452 732 627 385 367 366
346 186 86 17 12
BstZ17I GTA'TAC
Cuts at: 4167 4167
Size: 8338
Bsu36I CC'TnA_GG
Cuts at: 2030 3101 5616 2030
Size: 1071 2515 4752
Fragments arranged by size:
4752 2515 1071
BtgI C'CryG_G
Cuts at: 513 1065 2022 4107 4491 513
Size: 552 957 2085 384 4360
Fragments arranged by size:
4360 2085 957 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4465 487
Size: 683 56 3239 4360
Fragments arranged by size:
4360 3239 683 56
BtsI GCAGTG_nn'
Cuts at: 4134 4350 5116 5854 6648 6668 4134
Size: 216 766 738 794 20 5804
Fragments arranged by size:
5804 794 766 738 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3454 3489 529
Size: 35 2890 35 5378
Fragments arranged by size:
5378 2890 35 35
DraI TTT'AAA
Cuts at: 2779 2937 3334 3369 5642 2779
Size: 158 397 35 2273 5475
Fragments arranged by size:
5475 2273 397 158 35
DraIII CAC_nnn'GTG
Cuts at: 3004 4909 3004
Size: 1905 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7345 809
Size: 507 6029 1802
Fragments arranged by size:
6029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2624 2727 4827
Size: 54 1425 50 275 18 794 103 2100
Cuts at: 4827 5785 6674 8303 8
Size: 958 889 1629 43
Fragments arranged by size:
2100 1629 1425 958 889 794 275 103
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5785 1537
Size: 275 3973 4090
Fragments arranged by size:
4090 3973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3298 4680 4978 5175 6268 1353
Size: 1945 1382 298 197 1093 3423
Fragments arranged by size:
3423 1945 1382 1093 298 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6925 7299 7445 387
Size: 1064 138 5336 374 146 1280
Fragments arranged by size:
5336 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2592 3586 3846 3915 4772 4869
Size: 393 742 218 994 260 69 857 97
Cuts at: 4869 4978 5250 5310 5597 6401 6998 7785 8053
Size: 109 272 60 287 804 597 787 268
Cuts at: 8053 1239
Size: 1524
Fragments arranged by size:
1524 994 857 804 787 742 597 393
287 272 268 260 218 109 97 69
60
EcoNI CCTnn'n_nnAGG
Cuts at: 2068 2532 2068
Size: 464 7874
EcoO109I rG'GnC_Cy
Cuts at: 2970 3241 3666 4783 5038 5039 5273 2970
Size: 271 425 1117 255 1 234 6035
Fragments arranged by size:
6035 1117 425 271 255 234 1
EcoRI G'AATT_C
Cuts at: 3323 5195 5647 6025 3323
Size: 1872 452 378 5636
Fragments arranged by size:
5636 1872 452 378
EcoRV GAT'ATC
Cuts at: 2596 2596
Size: 8338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5059 5059
Size: 8338
FspI TGC'GCA
Cuts at: 6844 6844
Size: 8338
HaeII r_GCGC'y
Cuts at: 1678 2202 2325 5068 7485 1678
Size: 524 123 2743 2417 2531
Fragments arranged by size:
2743 2531 2417 524 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2580 2612 3014 3046 5195
Size: 32 135 32 1008 32 402 32 2149
Cuts at: 5195 5227 5228 5240 5260 5272 5293 5325 6979
Size: 32 1 12 20 12 21 32 1654
Cuts at: 6979 7011 7053 7085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2149 1654 1008 402 135 42 32
32 32 32 32 32 32 32 21
20 12 12 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4138 4282 4345 5682 5928 669
Size: 988 281 2200 144 63 1337 246 3079
Fragments arranged by size:
3079 2200 1337 988 281 246 144 63
HindIII A'AGCT_T
Cuts at: 748 3395 5435 748
Size: 2647 2040 3651
Fragments arranged by size:
3651 2647 2040
HpaI GTT'AAC
Cuts at: 4345 5928 4345
Size: 1583 6755
KasI G'GCGC_C
Cuts at: 2321 2321
Size: 8338
KpnI G_GTAC'C
Cuts at: 2829 4303 5663 2829
Size: 1474 1360 5504
Fragments arranged by size:
5504 1474 1360
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 3282 3861 4389 4820 7452 7636 1305
Size: 1008 969 579 528 431 2632 184 2007
Fragments arranged by size:
2632 2007 1008 969 579 528 431 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2626 4829 10
Size: 54 1425 343 794 2203 3519
Fragments arranged by size:
3519 2203 1425 794 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 3203 4145 4322 4505 4995 6296
Size: 648 1012 1025 942 177 183 490 1301
Cuts at: 6296 6655 6814 518
Size: 359 159 2042
Fragments arranged by size:
2042 1301 1025 1012 942 648 490 359
183 177 159
MspA1I CmG'CkG
Cuts at: 1606 5754 6431 7579 7824 1606
Size: 4148 677 1148 245 2120
Fragments arranged by size:
4148 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 4743 4743
Size: 8338
NarI GG'CG_CC
Cuts at: 2322 2322
Size: 8338
NcoI C'CATG_G
Cuts at: 513 1065 2022 4491 513
Size: 552 957 2469 4360
Fragments arranged by size:
4360 2469 957 552
NdeI CA'TA_TG
Cuts at: 387 2329 387
Size: 1942 6396
NgoMIV G'CCGG_C
Cuts at: 4741 4741
Size: 8338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NsiI A_TGCA'T
Cuts at: 4546 4546
Size: 8338
NspI r_CATG'y
Cuts at: 1132 1229 5696 7241 1132
Size: 97 4467 1545 2229
Fragments arranged by size:
4467 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8338
PciI A'CATG_T
Cuts at: 7237 7237
Size: 8338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5466 1108
Size: 4358 3980
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PmlI CAC'GTG
Cuts at: 4909 4909
Size: 8338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6407 6439 7920 7952 6407
Size: 32 1481 32 6793
Fragments arranged by size:
6793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2970 3241 3666 5273 2970
Size: 271 425 1607 6035
Fragments arranged by size:
6035 1607 425 271
PshAI GACnn'nnGTC
Cuts at: 1613 5118 1613
Size: 3505 4833
PsiI TTA'TAA
Cuts at: 3113 5586 5908 3113
Size: 2473 322 5543
Fragments arranged by size:
5543 2473 322
PspOMI G'GGCC_C
Cuts at: 4783 5038 4783
Size: 255 8083
PspXI vC'TCGA_Gb
Cuts at: 3042 3042
Size: 8338
PstI C_TGCA'G
Cuts at: 830 2739 5111 5690 830
Size: 1909 2372 579 3478
Fragments arranged by size:
3478 2372 1909 579
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8338
SalI G'TCGA_C
Cuts at: 1936 5680 1936
Size: 3744 4594
SanDI GG'GwC_CC
Cuts at: 3666 5273 3666
Size: 1607 6731
SapI GCTCTTCn'nnn_
Cuts at: 4978 4978
Size: 8338
SbfI CC_TGCA'GG
Cuts at: 5690 5690
Size: 8338
ScaI AGT'ACT
Cuts at: 3790 6586 8298 3790
Size: 2796 1712 3830
Fragments arranged by size:
3830 2796 1712
SfcI C'TryA_G
Cuts at: 826 1045 2212 2735 4170 5107 5295 5686 6821
Size: 219 1167 523 1435 937 188 391 1135
Cuts at: 6821 7502 7693 826
Size: 681 191 1471
Fragments arranged by size:
1471 1435 1167 1135 937 681 523 391
219 191 188
SfoI GGC'GCC
Cuts at: 2323 2323
Size: 8338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 4484 1700
Size: 2784 5554
SmaI CCC'GGG
Cuts at: 1872 5665 1872
Size: 3793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2091 2360 3042 3398 5310 6442
Size: 197 926 148 269 682 356 1912 1132
Cuts at: 6442 7343 7605 820
Size: 901 262 1553
Fragments arranged by size:
1912 1553 1132 926 901 682 356 269
262 197 148
SnaBI TAC'GTA
Cuts at: 493 5635 6129 493
Size: 5142 494 2702
Fragments arranged by size:
5142 2702 494
SpeI A'CTAG_T
Cuts at: 152 4979 152
Size: 4827 3511
SphI G_CATG'C
Cuts at: 1132 5696 1132
Size: 4564 3774
SspI AAT'ATT
Cuts at: 5 52 2467 3278 6262 5
Size: 47 2415 811 2984 2081
Fragments arranged by size:
2984 2415 2081 811 47
StuI AGG'CCT
Cuts at: 1517 5210 1517
Size: 3693 4645
StyI C'CwwG_G
Cuts at: 513 1065 2022 4491 5017 5777 513
Size: 552 957 2469 526 760 3074
Fragments arranged by size:
3074 2469 957 760 552 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3565 3591 5410 6365 6382 6535 6720
Size: 1255 1985 26 1819 955 17 153 185
Cuts at: 6720 7165 325
Size: 445 1498
Fragments arranged by size:
1985 1819 1498 1255 955 445 185 153
26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2548 3788 6584
Size: 274 80 33 51 1443 571 1240 2796
Cuts at: 6584 8296 96
Size: 1712 138
Fragments arranged by size:
2796 1712 1443 1240 571 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 8298 8330 2330
Size: 32 5936 32 2338
Fragments arranged by size:
5936 2338 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8338
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5537 5537
Size: 8338
XhoI C'TCGA_G
Cuts at: 1943 3042 1943
Size: 1099 7239
XmaI C'CCGG_G
Cuts at: 1870 5663 1870
Size: 3793 4545
XmnI GAAnn'nnTTC
Cuts at: 5492 6467 5492
Size: 975 7363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6146 276
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BsiWI BsmBI BstXI
EcoICRI FseI FspAI NruI PacI PfoI PsrI PvuII
RsrII SacI SacII SexAI SfiI SrfI SwaI