Restriction map for
pFN21ASDB0064
(Circular) (Six-base) MAPSORT of: pFN21ASDB0064.seq Check: 6055 from: 1 to: 7225
pFN21ASDB0064 7225 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5035 278
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4568 1937
Size: 2631 4594
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 4044 5352 5725 1563
Size: 2481 1308 373 3063
Fragments arranged by size:
3063 2481 1308 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2979 4389 5288 6672 6940 1632
Size: 1347 1410 899 1384 268 1917
Fragments arranged by size:
1917 1410 1384 1347 899 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7028
AflIII A'CryG_T
Cuts at: 1203 2998 3901 6124 7101 1203
Size: 1795 903 2223 977 1327
Fragments arranged by size:
2223 1795 1327 977 903
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 5954 5954
Size: 7225
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4157 1184
Size: 2973 4252
AlwNI CAG_nnn'CTG
Cuts at: 4210 4968 6540 4210
Size: 758 1572 4895
Fragments arranged by size:
4895 1572 758
ApaI G_GGCC'C
Cuts at: 3329 3329
Size: 7225
ApaLI G'TGCA_C
Cuts at: 5282 6438 5282
Size: 1156 6069
ApoI r'AATT_y
Cuts at: 1493 2118 2381 3883 3965 3985 4534 4760 4912
Size: 625 263 1502 82 20 549 226 152
Cuts at: 4912 7021 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1502 625 549 263 226 152
82 20
AseI AT'TA_AT
Cuts at: 160 1032 2724 5779 160
Size: 872 1692 3055 1606
Fragments arranged by size:
3055 1692 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 2515 3322 4550 1870
Size: 73 360 212 807 1228 4545
Fragments arranged by size:
4545 1228 807 360 212 73
BamHI G'GATC_C
Cuts at: 2825 4555 4922 2825
Size: 1730 367 5128
Fragments arranged by size:
5128 1730 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2221 4546 6001 618
Size: 325 217 627 434 2325 1455 1842
Fragments arranged by size:
2325 1842 1455 627 434 325 217
BanII G_rGCy'C
Cuts at: 1391 2185 3329 3703 4544 1391
Size: 794 1144 374 841 4072
Fragments arranged by size:
4072 1144 841 794 374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2678 928
Size: 971 251 528 5475
Fragments arranged by size:
5475 971 528 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 5416 5450 1903
Size: 34 1826 34 1619 34 3678
Fragments arranged by size:
3678 1826 1619 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6327 5117
Size: 1210 6015
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7225
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3090 3127 5836
Size: 107 122 71 631 605 1418 37 2709
Cuts at: 5836 136
Size: 1525
Fragments arranged by size:
2709 1525 1418 631 605 122 107 71
37
BglII A'GATC_T
Cuts at: 7220 7220
Size: 7225
BlpI GC'TnA_GC
Cuts at: 3186 4642 3186
Size: 1456 5769
Bme1580I G_kGCm'C
Cuts at: 1790 2224 3329 5286 6442 1790
Size: 434 1105 1957 1156 2573
Fragments arranged by size:
2573 1957 1156 1105 434
BmgBI CAC'GTC
Cuts at: 1318 3904 1318
Size: 2586 4639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4956 5914 442
Size: 416 53 83 399 24 3539 958 1753
Fragments arranged by size:
3539 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2565 2589 3063 3435 3483 4226 5885 1239
Size: 1326 24 474 372 48 743 1659 2579
Fragments arranged by size:
2579 1659 1326 743 474 372 48 24
Bpu10I CC'TnA_GC
Cuts at: 2271 3532 3702 2271
Size: 1261 170 5794
Fragments arranged by size:
5794 1261 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 5350 6215 6513 3598
Size: 1752 865 298 4310
Fragments arranged by size:
4310 1752 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5888 882
Size: 617 4389 2219
Fragments arranged by size:
4389 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4522 5016 493
Size: 711 3318 494 2702
Fragments arranged by size:
3318 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7219 7219
Size: 7225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5032 5414 6049 275
Size: 53 83 186 936 3499 382 635 1451
Fragments arranged by size:
3499 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3007 3794 5658 6056 6330 6477 6888 3007
Size: 787 1864 398 274 147 411 3344
Fragments arranged by size:
3344 1864 787 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2563 2593 3253 3283 3394
Size: 30 165 30 1160 30 660 30 111
Cuts at: 3394 3424 3789 3819 1178
Size: 30 365 30 4584
Fragments arranged by size:
4584 1160 660 365 165 111 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2619 3123 3408 3507 4242 1186
Size: 961 472 504 285 99 735 4169
Fragments arranged by size:
4169 961 735 504 472 285 99
BseYI C'CCAG_C
Cuts at: 3061 3073 3680 3856 6428 6924 3061
Size: 12 607 176 2572 496 3362
Fragments arranged by size:
3362 2572 607 496 176 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4223 4421 1163
Size: 573 2487 198 3967
Fragments arranged by size:
3967 2487 573 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4675 5436 6464 1540
Size: 275 186 2674 761 1028 2301
Fragments arranged by size:
2674 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3703 4254 5286 5371 6442 3703
Size: 551 1032 85 1071 4486
Fragments arranged by size:
4486 1071 1032 551 85
BsmI GAATG_Cn'
Cuts at: 2434 4080 4276 4736 4829 2434
Size: 1646 196 460 93 4830
Fragments arranged by size:
4830 1646 460 196 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2185 2224 3329 3703 4254 4544 5286
Size: 399 395 39 1105 374 551 290 742
Cuts at: 5286 5371 6442 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 551 399 395 374
290 85 39
BspEI T'CCGG_A
Cuts at: 3007 6888 3007
Size: 3881 3344
BspHI T'CATG_A
Cuts at: 5114 6844 5114
Size: 1730 5495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3882 4149 4580 844
Size: 3038 267 431 3489
Fragments arranged by size:
3489 3038 431 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3154 5112 1394
Size: 429 1331 1958 3507
Fragments arranged by size:
3507 1958 1331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2221 2402 3145 4179 5720 5894 65
Size: 1183 973 181 743 1034 1541 174 1396
Fragments arranged by size:
1541 1396 1183 1034 973 743 181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5869 6056 1700
Size: 110 4059 187 2869
Fragments arranged by size:
4059 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7225
BssSI C'ACGA_G
Cuts at: 5285 6297 5285
Size: 1012 6213
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7225
BstBI TT'CG_AA
Cuts at: 2007 6029 2007
Size: 4022 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7225
BstXI CCAn_nnnn'nTGG
Cuts at: 3382 4266 3382
Size: 884 6341
BstYI r'GATC_y
Cuts at: 1972 2825 4555 4922 5307 5324 6776 6862 6874
Size: 853 1730 367 385 17 1452 86 12
Cuts at: 6874 7220 1972
Size: 346 1977
Fragments arranged by size:
1977 1730 1452 853 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3693 513
Size: 552 2628 4045
Fragments arranged by size:
4045 2628 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 4132 487
Size: 683 56 1229 1677 3580
Fragments arranged by size:
3580 1677 1229 683 56
BtsI GCAGTG_nn'
Cuts at: 2783 4113 4254 4741 5535 5555 2783
Size: 1330 141 487 794 20 4453
Fragments arranged by size:
4453 1330 794 487 141 20
ClaI AT'CG_AT
Cuts at: 1554 4465 1554
Size: 2911 4314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7190
DraI TTT'AAA
Cuts at: 4529 4529
Size: 7225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4229 6232 809
Size: 507 2913 2003 1802
Fragments arranged by size:
2913 2003 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3437 4672 5561
Size: 54 1425 50 275 18 1607 1235 889
Cuts at: 5561 7190 8
Size: 1629 43
Fragments arranged by size:
1629 1607 1425 1235 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4672 1537
Size: 275 2860 4090
Fragments arranged by size:
4090 2860 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5155 1353
Size: 3802 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5812 6186 6332 387
Size: 1064 138 4223 374 146 1280
Fragments arranged by size:
4223 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3701 3701
Size: 7225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2565 2589 2979 3063 3435 3483 4226
Size: 393 933 24 390 84 372 48 743
Cuts at: 4226 4389 5288 5885 6672 6940 1239
Size: 163 899 597 787 268 1524
Fragments arranged by size:
1524 933 899 787 743 597 393 390
372 268 163 84 48 24
EcoO109I rG'GnC_Cy
Cuts at: 3083 3101 3110 3119 3326 3677 3083
Size: 18 9 9 207 351 6631
Fragments arranged by size:
6631 351 207 18 9 9
EcoRI G'AATT_C
Cuts at: 2118 3965 4534 4912 2118
Size: 1847 569 378 4431
Fragments arranged by size:
4431 1847 569 378
EcoRV GAT'ATC
Cuts at: 4309 4309
Size: 7225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2438 3570 4401 2438
Size: 1132 831 5262
Fragments arranged by size:
5262 1132 831
FspI TGC'GCA
Cuts at: 4145 5731 4145
Size: 1586 5639
HaeII r_GCGC'y
Cuts at: 1678 6372 1678
Size: 4694 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 4458 4490 5866
Size: 32 135 32 913 32 1941 32 1376
Cuts at: 5866 5898 5940 5972 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1941 1376 913 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2193 2751 3015 3567 4048 4569
Size: 988 281 255 558 264 552 481 521
Cuts at: 4569 4815 669
Size: 246 3079
Fragments arranged by size:
3079 988 558 552 521 481 281 264
255 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7225
HpaI GTT'AAC
Cuts at: 2193 2751 4815 2193
Size: 558 2064 4603
Fragments arranged by size:
4603 2064 558
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 2838 4824 2838
Size: 1986 5239
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3448 4432 6339 6523 1305
Size: 2143 984 1907 184 2007
Fragments arranged by size:
2143 2007 1907 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5403
Fragments arranged by size:
5403 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5183 5542 5701 518
Size: 648 4017 359 159 2042
Fragments arranged by size:
4017 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3065 3860 4641 5318 6466 6711 1606
Size: 1459 795 781 677 1148 245 2120
Fragments arranged by size:
2120 1459 1148 795 781 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3693 513
Size: 552 2628 4045
Fragments arranged by size:
4045 2628 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7225
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NspI r_CATG'y
Cuts at: 1132 1229 3002 3982 4054 4583 6128 1132
Size: 97 1773 980 72 529 1545 2229
Fragments arranged by size:
2229 1773 1545 980 529 97 72
PasI CC'CwG_GG
Cuts at: 1348 3116 4075 1348
Size: 1768 959 4498
Fragments arranged by size:
4498 1768 959
PciI A'CATG_T
Cuts at: 2998 6124 2998
Size: 3126 4099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7225
PfoI T'CCnGG_A
Cuts at: 3451 3451
Size: 7225
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5294 5326 6807 6839 5294
Size: 32 1481 32 5680
Fragments arranged by size:
5680 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3101 3677 3101
Size: 576 6649
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7225
PsiI TTA'TAA
Cuts at: 2591 4041 4795 2591
Size: 1450 754 5021
Fragments arranged by size:
5021 1450 754
PspOMI G'GGCC_C
Cuts at: 3325 3325
Size: 7225
PstI C_TGCA'G
Cuts at: 830 4498 4577 830
Size: 3668 79 3478
Fragments arranged by size:
3668 3478 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 3065 3860 3065
Size: 795 6430
SacI G_AGCT'C
Cuts at: 3703 3703
Size: 7225
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SanDI GG'GwC_CC
Cuts at: 3677 3677
Size: 7225
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 5473 7185 5473
Size: 1712 5513
SfcI C'TryA_G
Cuts at: 826 1045 2094 2290 2439 3289 4494 4573 5708
Size: 219 1049 196 149 850 1205 79 1135
Cuts at: 5708 6389 6580 826
Size: 681 191 1471
Fragments arranged by size:
1471 1205 1135 1049 850 681 219 196
191 149 79
SfiI GGCCn_nnn'nGGCC
Cuts at: 3090 3127 3090
Size: 37 7188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7225
SmaI CCC'GGG
Cuts at: 1872 2517 4552 1872
Size: 645 2035 4545
Fragments arranged by size:
4545 2035 645
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 5329 6230 6492 820
Size: 197 926 1634 1752 901 262 1553
Fragments arranged by size:
1752 1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4522 5016 493
Size: 4029 494 2702
Fragments arranged by size:
4029 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 4583 1132
Size: 3451 3774
SspI AAT'ATT
Cuts at: 5 52 4290 5149 5
Size: 47 4238 859 2081
Fragments arranged by size:
4238 2081 859 47
StuI AGG'CCT
Cuts at: 1517 2057 2758 1517
Size: 540 701 5984
Fragments arranged by size:
5984 701 540
StyI C'CwwG_G
Cuts at: 513 1065 3020 3693 3960 4664 513
Size: 552 1955 673 267 704 3074
Fragments arranged by size:
3074 1955 704 673 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3298 5252 5269 5422 5607 6052 325
Size: 1255 1718 1954 17 153 185 445 1498
Fragments arranged by size:
1954 1718 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3142 5471 7183
Size: 274 80 33 51 1443 1165 2329 1712
Cuts at: 7183 96
Size: 138
Fragments arranged by size:
2329 1712 1443 1165 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2563 2595 3331 3363 3394 3426 3789 3821 7185
Size: 32 736 32 31 32 363 32 3364
Cuts at: 7185 7217 2563
Size: 32 2571
Fragments arranged by size:
3364 2571 736 363 32 32 32 32
32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7225
XbaI T'CTAG_A
Cuts at: 4561 4561
Size: 7225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2902 4263 2548
Size: 354 1361 5510
Fragments arranged by size:
5510 1361 354
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7225
XmaI C'CCGG_G
Cuts at: 1870 2515 4550 1870
Size: 645 2035 4545
Fragments arranged by size:
4545 2035 645
XmnI GAAnn'nnTTC
Cuts at: 5354 5354
Size: 7225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5033 276
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfoI SrfI SwaI