Restriction map for
pFN21ASDB0061
(Circular) (Six-base) MAPSORT of: pFN21ASDB0061.seq Check: 6156 from: 1 to: 8023
pFN21ASDB0061 8023 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3996 3996
Size: 8023
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3561 5833 278
Size: 53 83 186 936 2025 2272 2468
Fragments arranged by size:
2468 2272 2025 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5366 1937
Size: 3429 4594
Acc65I G'GTAC_C
Cuts at: 5344 5344
Size: 8023
AclI AA'CG_TT
Cuts at: 1563 6150 6523 1563
Size: 4587 373 3063
Fragments arranged by size:
4587 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3299 3872 4618 6086 7470 7738 1632
Size: 1667 573 746 1468 1384 268 1917
Fragments arranged by size:
1917 1667 1468 1384 746 573 268
AfeI AGC'GCT
Cuts at: 2039 2141 4595 2039
Size: 102 2454 5467
Fragments arranged by size:
5467 2454 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7826
AflIII A'CryG_T
Cuts at: 1203 2298 3411 6922 7899 1203
Size: 1095 1113 3511 977 1327
Fragments arranged by size:
3511 1327 1113 1095 977
AgeI A'CCGG_T
Cuts at: 6854 6854
Size: 8023
AhdI GACnn_n'nnGTC
Cuts at: 5148 6752 5148
Size: 1604 6419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3188 3939 4910 1184
Size: 2004 751 971 4297
Fragments arranged by size:
4297 2004 971 751
AlwNI CAG_nnn'CTG
Cuts at: 2662 2695 2836 2886 3009 3457 3469 3988 4288
Size: 33 141 50 123 448 12 519 300
Cuts at: 4288 4477 5111 5766 7338 2662
Size: 189 634 655 1572 3347
Fragments arranged by size:
3347 1572 655 634 519 448 300 189
141 123 50 33 12
ApaI G_GGCC'C
Cuts at: 4919 4973 5167 4919
Size: 54 194 7775
Fragments arranged by size:
7775 194 54
ApaLI G'TGCA_C
Cuts at: 3751 6080 7236 3751
Size: 2329 1156 4538
Fragments arranged by size:
4538 2329 1156
ApoI r'AATT_y
Cuts at: 1493 3908 5039 5332 5558 5710 7819 1493
Size: 2415 1131 293 226 152 2109 1697
Fragments arranged by size:
2415 2109 1697 1131 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6577 160
Size: 872 5545 1606
Fragments arranged by size:
5545 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
AvaI C'yCGr_G
Cuts at: 1870 1943 2474 2907 3656 3801 3938 4271 4851
Size: 73 531 433 749 145 137 333 580
Cuts at: 4851 4965 5007 5248 5348 1870
Size: 114 42 241 100 4545
Fragments arranged by size:
4545 749 580 531 433 333 241 145
137 114 100 73 42
BamHI G'GATC_C
Cuts at: 2814 5353 5720 2814
Size: 2539 367 5117
Fragments arranged by size:
5117 2539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3684 4137 4685 4894 4949
Size: 325 217 627 1897 453 548 209 55
Cuts at: 4949 5157 5344 6799 618
Size: 208 187 1455 1842
Fragments arranged by size:
1897 1842 1455 627 548 453 325 217
209 208 187 55
BanII G_rGCy'C
Cuts at: 1391 3093 3383 4014 4790 4919 4973 5167 5342
Size: 1702 290 631 776 129 54 194 175
Cuts at: 5342 1391
Size: 4072
Fragments arranged by size:
4072 1702 776 631 290 194 175 129
54
BbeI G_GCGC'C
Cuts at: 3688 4141 3688
Size: 453 7570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 2375 2898 4552 928
Size: 971 219 257 523 1654 4399
Fragments arranged by size:
4399 1654 971 523 257 219
BbvCI CC'TCA_GC
Cuts at: 4493 4493
Size: 8023
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3217 3251 3974 4008 4091 4125 4579
Size: 34 1280 34 723 34 83 34 454
Cuts at: 4579 4613 6214 6248 1903
Size: 34 1601 34 3678
Fragments arranged by size:
3678 1601 1280 723 454 83 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5915 7125 5915
Size: 1210 6813
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8023
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 3012 3387
Size: 107 122 71 631 605 854 486 375
Cuts at: 3387 3955 5283 6634 136
Size: 568 1328 1351 1525
Fragments arranged by size:
1525 1351 1328 854 631 605 568 486
375 122 107 71
BglII A'GATC_T
Cuts at: 2580 3195 5313 8018 2580
Size: 615 2118 2705 2585
Fragments arranged by size:
2705 2585 2118 615
BlpI GC'TnA_GC
Cuts at: 3377 5201 5440 3377
Size: 1824 239 5960
Fragments arranged by size:
5960 1824 239
Bme1580I G_kGCm'C
Cuts at: 1790 3755 4899 4919 4952 4973 5014 5167 6084
Size: 1965 1144 20 33 21 41 153 917
Cuts at: 6084 7240 1790
Size: 1156 2573
Fragments arranged by size:
2573 1965 1156 1144 917 153 41 33
21 20
BmgBI CAC'GTC
Cuts at: 1318 2440 3412 3945 5015 1318
Size: 1122 972 533 1070 4326
Fragments arranged by size:
4326 1122 1070 972 533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2251 3817 5754
Size: 416 53 83 399 24 834 1566 1937
Cuts at: 5754 6712 442
Size: 958 1753
Fragments arranged by size:
1937 1753 1566 958 834 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 4508 3608
Size: 900 7123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 3529 4097 4280 4571 4949 4962 6683
Size: 1379 911 568 183 291 378 13 1721
Cuts at: 6683 1239
Size: 2579
Fragments arranged by size:
2579 1721 1379 911 568 378 291 183
13
Bpu10I CC'TnA_GC
Cuts at: 4493 4493
Size: 8023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4718 6148 7013 7311 4718
Size: 1430 865 298 5430
Fragments arranged by size:
5430 1430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3553 3782 6686 882
Size: 617 2054 229 2904 2219
Fragments arranged by size:
2904 2219 2054 617 229
BsaAI yAC'GTr
Cuts at: 493 1204 4144 5179 5305 5320 5814 493
Size: 711 2940 1035 126 15 494 2702
Fragments arranged by size:
2940 2702 1035 711 494 126 15
BsaBI GATnn'nnATC
Cuts at: 8017 8017
Size: 8023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3558 3685 3978
Size: 53 83 186 936 582 1443 127 293
Cuts at: 3978 4138 4376 5830 6212 6847 275
Size: 160 238 1454 382 635 1451
Fragments arranged by size:
1454 1451 1443 936 635 582 382 293
238 186 160 127 83 53
BsaWI w'CCGG_w
Cuts at: 2133 2304 2710 3642 4152 6456 6854 7128 7275
Size: 171 406 932 510 2304 398 274 147
Cuts at: 7275 7686 2133
Size: 411 2470
Fragments arranged by size:
2470 2304 932 510 411 406 398 274
171 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4922 4952 1178
Size: 30 165 30 3519 30 4249
Fragments arranged by size:
4249 3519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3308 3827 4094 4178 4277 4616 1186
Size: 2122 519 267 84 99 339 4593
Fragments arranged by size:
4593 2122 519 339 267 99 84
BseYI C'CCAG_C
Cuts at: 2363 2822 2950 3009 3689 4671 5133 7226 7722
Size: 459 128 59 680 982 462 2093 496
Cuts at: 7722 2363
Size: 2664
Fragments arranged by size:
2664 2093 982 680 496 462 459 128
59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3323 1163
Size: 573 1587 5863
Fragments arranged by size:
5863 1587 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2227 4339 4747 5473 6234 7262
Size: 275 186 226 2112 408 726 761 1028
Cuts at: 7262 1540
Size: 2301
Fragments arranged by size:
2301 2112 1028 761 726 408 275 226
186
BsiHKAI G_wGCw'C
Cuts at: 2184 3755 4037 4734 4790 5031 6084 6169 7240
Size: 1571 282 697 56 241 1053 85 1071
Cuts at: 7240 2184
Size: 2967
Fragments arranged by size:
2967 1571 1071 1053 697 282 241 85
56
BsmI GAATG_Cn'
Cuts at: 5534 5627 5534
Size: 93 7930
BsmBI CGTCTCn'nnnn_
Cuts at: 3579 3935 3579
Size: 356 7667
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 3093 3383 3755 4014 4037 4734
Size: 399 394 909 290 372 259 23 697
Cuts at: 4734 4790 4899 4919 4952 4973 5014 5031 5167
Size: 56 109 20 33 21 41 17 136
Cuts at: 5167 5342 6084 6169 7240 1391
Size: 175 742 85 1071 2174
Fragments arranged by size:
2174 1071 909 742 697 399 394 372
290 259 175 136 109 85 56 41
33 23 21 20 17
BspEI T'CCGG_A
Cuts at: 2133 3642 4152 7686 2133
Size: 1509 510 3534 2470
Fragments arranged by size:
3534 2470 1509 510
BspHI T'CATG_A
Cuts at: 2703 3393 5912 7642 2703
Size: 690 2519 1730 3084
Fragments arranged by size:
3084 2519 1730 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2794 2955 3996 5378 844
Size: 1950 161 1041 1382 3489
Fragments arranged by size:
3489 1950 1382 1041 161
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 4210 4267 4303 5910 1394
Size: 429 250 2137 57 36 1607 3507
Fragments arranged by size:
3507 2137 1607 429 250 57 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 4894 6518 6692 65
Size: 1183 870 2776 1624 174 1396
Fragments arranged by size:
2776 1624 1396 1183 870 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3515 3698 3878 5069 5265 6667 6854
Size: 110 1705 183 180 1191 196 1402 187
Cuts at: 6854 1700
Size: 2869
Fragments arranged by size:
2869 1705 1402 1191 196 187 183 180
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8023
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8023
BssSI C'ACGA_G
Cuts at: 2849 3116 3491 6083 7095 2849
Size: 267 375 2592 1012 3777
Fragments arranged by size:
3777 2592 1012 375 267
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3469 794
Size: 2675 5348
BstBI TT'CG_AA
Cuts at: 2007 6827 2007
Size: 4820 3203
BstEII G'GTnAC_C
Cuts at: 1184 4324 1184
Size: 3140 4883
BstXI CCAn_nnnn'nTGG
Cuts at: 3209 3539 3730 4879 4987 3209
Size: 330 191 1149 108 6245
Fragments arranged by size:
6245 1149 330 191 108
BstYI r'GATC_y
Cuts at: 1972 2541 2580 2814 2859 3195 5229 5242 5313
Size: 569 39 234 45 336 2034 13 71
Cuts at: 5313 5353 5720 6105 6122 7574 7660 7672 8018
Size: 40 367 385 17 1452 86 12 346
Cuts at: 8018 1972
Size: 1977
Fragments arranged by size:
2034 1977 1452 569 385 367 346 336
234 86 71 45 40 39 17 13
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3432 3950 4708 513
Size: 552 957 1410 518 758 3828
Fragments arranged by size:
3828 1410 957 758 552 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4785 487
Size: 683 56 3559 3725
Fragments arranged by size:
3725 3559 683 56
BtsI GCAGTG_nn'
Cuts at: 3455 4734 5539 6333 6353 3455
Size: 1279 805 794 20 5125
Fragments arranged by size:
5125 1279 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7988
DraI TTT'AAA
Cuts at: 5327 5327
Size: 8023
DraIII CAC_nnn'GTG
Cuts at: 2062 3835 2062
Size: 1773 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7030 809
Size: 507 5714 1802
Fragments arranged by size:
5714 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3081 3735 3947
Size: 54 1425 50 275 18 1251 654 212
Cuts at: 3947 4812 5067 5102 5275 5470 6359 7988 8
Size: 865 255 35 173 195 889 1629 43
Fragments arranged by size:
1629 1425 1251 889 865 654 275 255
212 195 173 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 5470 1537
Size: 275 3658 4090
Fragments arranged by size:
4090 3658 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 3150 3599 3656 5953 1353
Size: 1757 40 449 57 2297 3423
Fragments arranged by size:
3423 2297 1757 449 57 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2433 5007 6610 6984 7130 387
Size: 1064 138 844 2574 1603 374 146 1280
Fragments arranged by size:
2574 1603 1280 1064 844 374 146 138
EcoICRI GAG'CTC
Cuts at: 4788 4788
Size: 8023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 3299 3529 3872 4097 4280 4571
Size: 393 986 681 230 343 225 183 291
Cuts at: 4571 4618 4949 4962 6086 6683 7470 7738 1239
Size: 47 331 13 1124 597 787 268 1524
Fragments arranged by size:
1524 1124 986 787 681 597 393 343
331 291 268 230 225 183 47 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 3083 3119 4076 4079 4526 2243
Size: 840 36 957 3 447 5740
Fragments arranged by size:
5740 957 840 447 36 3
EcoO109I rG'GnC_Cy
Cuts at: 2670 3486 3798 4421 4617 4915 4916 4969 4970
Size: 816 312 623 196 298 1 53 1
Cuts at: 4970 5164 5284 2670
Size: 194 120 5409
Fragments arranged by size:
5409 816 623 312 298 196 194 120
53 1 1
EcoRI G'AATT_C
Cuts at: 5039 5332 5710 5039
Size: 293 378 7352
Fragments arranged by size:
7352 378 293
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 8023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 4381 2056
Size: 2325 5698
FspI TGC'GCA
Cuts at: 2504 3872 6529 2504
Size: 1368 2657 3998
Fragments arranged by size:
3998 2657 1368
HaeII r_GCGC'y
Cuts at: 1678 2041 2143 3516 3688 4141 4597 7170 1678
Size: 363 102 1373 172 453 456 2573 2531
Fragments arranged by size:
2573 2531 1373 456 453 363 172 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4922 4954 5134 5166 6664
Size: 32 135 32 3350 32 180 32 1498
Cuts at: 6664 6696 6738 6770 1373
Size: 32 42 32 2626
Fragments arranged by size:
3350 2626 1498 180 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4003 5367 5613 669
Size: 988 281 2065 1364 246 3079
Fragments arranged by size:
3079 2065 1364 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4179 748
Size: 3431 4592
HpaI GTT'AAC
Cuts at: 5613 5613
Size: 8023
KasI G'GCGC_C
Cuts at: 3684 4137 3684
Size: 453 7570
KpnI G_GTAC'C
Cuts at: 5348 5348
Size: 8023
MfeI C'AATT_G
Cuts at: 5622 5622
Size: 8023
MluI A'CGCG_T
Cuts at: 7899 7899
Size: 8023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2610 3624 4209 4727 7137 7321 1305
Size: 1182 123 1014 585 518 2410 184 2007
Fragments arranged by size:
2410 2007 1182 1014 585 518 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3083 3737 4814 10
Size: 54 1425 343 1251 654 1077 3219
Fragments arranged by size:
3219 1425 1251 1077 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3170 3743 3866 5981 6340 6499
Size: 648 1869 135 573 123 2115 359 159
Cuts at: 6499 518
Size: 2042
Fragments arranged by size:
2115 2042 1869 648 573 359 159 135
123
MspA1I CmG'CkG
Cuts at: 1606 2470 2659 2836 2964 3009 3318 3507 3564
Size: 864 189 177 128 45 309 189 57
Cuts at: 3564 3771 3952 4710 5439 6116 7264 7509 1606
Size: 207 181 758 729 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 758 729 677 309 245
207 189 189 181 177 128 57 45
NaeI GCC'GGC
Cuts at: 3517 3700 3880 5071 5267 3517
Size: 183 180 1191 196 6273
Fragments arranged by size:
6273 1191 196 183 180
NarI GG'CG_CC
Cuts at: 3685 4138 3685
Size: 453 7570
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6514
Fragments arranged by size:
6514 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8023
NgoMIV G'CCGG_C
Cuts at: 3515 3698 3878 5069 5265 3515
Size: 183 180 1191 196 6273
Fragments arranged by size:
6273 1191 196 183 180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8023
NotI GC'GGCC_GC
Cuts at: 5470 5470
Size: 8023
NspI r_CATG'y
Cuts at: 1132 1229 2302 2314 5077 5381 6926 1132
Size: 97 1073 12 2763 304 1545 2229
Fragments arranged by size:
2763 2229 1545 1073 304 97 12
PasI CC'CwG_GG
Cuts at: 1348 4987 1348
Size: 3639 4384
PciI A'CATG_T
Cuts at: 2298 6922 2298
Size: 4624 3399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 2836 5065 5111 5179 5191 1108
Size: 1554 174 2229 46 68 12 3940
Fragments arranged by size:
3940 2229 1554 174 68 46 12
PfoI T'CCnGG_A
Cuts at: 4074 4548 4074
Size: 474 7549
PmeI GTTT'AAAC
Cuts at: 5327 5327
Size: 8023
PmlI CAC'GTG
Cuts at: 4144 4144
Size: 8023
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 4082 4114 6092 6124 7605 7637 2531
Size: 32 1519 32 1978 32 1481 32 2917
Fragments arranged by size:
2917 1978 1519 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3486 4421 3486
Size: 935 7088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8023
PsiI TTA'TAA
Cuts at: 5593 5593
Size: 8023
PspOMI G'GGCC_C
Cuts at: 4915 4969 5163 4915
Size: 54 194 7775
Fragments arranged by size:
7775 194 54
PspXI vC'TCGA_Gb
Cuts at: 2474 2474
Size: 8023
PstI C_TGCA'G
Cuts at: 830 2460 2847 2976 3255 3465 3474 3918 5375
Size: 1630 387 129 279 210 9 444 1457
Cuts at: 5375 830
Size: 3478
Fragments arranged by size:
3478 1630 1457 444 387 279 210 129
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8023
PvuII CAG'CTG
Cuts at: 2659 2836 3507 2659
Size: 177 671 7175
Fragments arranged by size:
7175 671 177
SacI G_AGCT'C
Cuts at: 4790 4790
Size: 8023
SacII CC_GC'GG
Cuts at: 3953 4711 3953
Size: 758 7265
SalI G'TCGA_C
Cuts at: 1936 5365 1936
Size: 3429 4594
SanDI GG'GwC_CC
Cuts at: 3486 3486
Size: 8023
SapI GCTCTTCn'nnn_
Cuts at: 3656 3656
Size: 8023
SbfI CC_TGCA'GG
Cuts at: 5375 5375
Size: 8023
ScaI AGT'ACT
Cuts at: 2534 3335 6271 7983 2534
Size: 801 2936 1712 2574
Fragments arranged by size:
2936 2574 1712 801
SexAI A'CCwGG_T
Cuts at: 2748 3729 3831 2748
Size: 981 102 6940
Fragments arranged by size:
6940 981 102
SfcI C'TryA_G
Cuts at: 826 1045 2456 2843 2972 3251 3461 3470 3914
Size: 219 1411 387 129 279 210 9 444
Cuts at: 3914 4646 5371 6506 7187 7378 826
Size: 732 725 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 732 725 681 444 387
279 219 210 191 129 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5283 5283
Size: 8023
SfoI GGC'GCC
Cuts at: 3686 4139 3686
Size: 453 7570
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8023
SmaI CCC'GGG
Cuts at: 1872 4853 4967 5009 5350 1872
Size: 2981 114 42 341 4545
Fragments arranged by size:
4545 2981 341 114 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 4733 6127 7028 7290 820
Size: 197 926 531 2259 1394 901 262 1553
Fragments arranged by size:
2259 1553 1394 926 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 5320 5814 493
Size: 4827 494 2702
Fragments arranged by size:
4827 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8023
SphI G_CATG'C
Cuts at: 1132 5077 5381 1132
Size: 3945 304 3774
Fragments arranged by size:
3945 3774 304
SspI AAT'ATT
Cuts at: 5 52 5947 5
Size: 47 5895 2081
Fragments arranged by size:
5895 2081 47
StuI AGG'CCT
Cuts at: 1517 2480 3635 4130 4960 1517
Size: 963 1155 495 830 4580
Fragments arranged by size:
4580 1155 963 830 495
StyI C'CwwG_G
Cuts at: 513 1065 2022 2406 3084 4038 4815 5462 513
Size: 552 957 384 678 954 777 647 3074
Fragments arranged by size:
3074 957 954 777 678 647 552 384
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4312 4583 4967 6050 6067 6220 6405
Size: 1255 2732 271 384 1083 17 153 185
Cuts at: 6405 6850 325
Size: 445 1498
Fragments arranged by size:
2732 1498 1255 1083 445 384 271 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 3159 3333
Size: 274 80 33 51 1443 555 627 174
Cuts at: 3333 6269 7981 96
Size: 2936 1712 138
Fragments arranged by size:
2936 1712 1443 627 555 274 174 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 5289 5321 7983 8015 3398
Size: 32 1859 32 2662 32 3406
Fragments arranged by size:
3406 2662 1859 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8023
XbaI T'CTAG_A
Cuts at: 5359 5359
Size: 8023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2593 2995 3536 3730 2389
Size: 204 402 541 194 6682
Fragments arranged by size:
6682 541 402 204 194
XhoI C'TCGA_G
Cuts at: 1943 2474 1943
Size: 531 7492
XmaI C'CCGG_G
Cuts at: 1870 4851 4965 5007 5348 1870
Size: 2981 114 42 341 4545
Fragments arranged by size:
4545 2981 341 114 42
XmnI GAAnn'nnTTC
Cuts at: 2141 6152 2141
Size: 4011 4012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3559 5831 276
Size: 53 83 186 936 2025 2272 2468
Fragments arranged by size:
2468 2272 2025 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
FseI FspAI NruI NsiI PacI PsrI RsrII SrfI
SwaI