Restriction map for
pFN21ASDB0060
(Circular) (Six-base) MAPSORT of: pFN21ASDB0060.seq Check: 3949 from: 1 to: 7450
pFN21ASDB0060 7450 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5260 278
Size: 53 83 186 936 3724 2468
Fragments arranged by size:
3724 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 4793 1937
Size: 1476 1380 4594
Fragments arranged by size:
4594 1476 1380
Acc65I G'GTAC_C
Cuts at: 3714 4715 4771 3714
Size: 1001 56 6393
Fragments arranged by size:
6393 1001 56
AclI AA'CG_TT
Cuts at: 1563 2052 5577 5950 1563
Size: 489 3525 373 3063
Fragments arranged by size:
3525 3063 489 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3176 3919 4361 4639 5513 6897 7165 1632
Size: 1544 743 442 278 874 1384 268 1917
Fragments arranged by size:
1917 1544 1384 874 743 442 278 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7253
AflIII A'CryG_T
Cuts at: 1203 4074 4740 6349 7326 1203
Size: 2871 666 1609 977 1327
Fragments arranged by size:
2871 1609 1327 977 666
AgeI A'CCGG_T
Cuts at: 6281 6281
Size: 7450
AhdI GACnn_n'nnGTC
Cuts at: 2041 6179 2041
Size: 4138 3312
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7450
AlwNI CAG_nnn'CTG
Cuts at: 2068 2234 5193 6765 2068
Size: 166 2959 1572 2753
Fragments arranged by size:
2959 2753 1572 166
ApaLI G'TGCA_C
Cuts at: 5507 6663 5507
Size: 1156 6294
ApoI r'AATT_y
Cuts at: 1493 2174 3085 3356 3493 3819 4526 4759 4985
Size: 681 911 271 137 326 707 233 226
Cuts at: 4985 5137 7246 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 911 707 681 326 271 233
226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 6004 160
Size: 872 4972 1606
Fragments arranged by size:
4972 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7450
AvaI C'yCGr_G
Cuts at: 1870 1943 3981 4775 1870
Size: 73 2038 794 4545
Fragments arranged by size:
4545 2038 794 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4573 4606 4573
Size: 33 7417
BamHI G'GATC_C
Cuts at: 2079 4780 5147 2079
Size: 2701 367 4382
Fragments arranged by size:
4382 2701 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3117 3714 4715 4771 6226
Size: 325 217 627 1330 597 1001 56 1455
Cuts at: 6226 618
Size: 1842
Fragments arranged by size:
1842 1455 1330 1001 627 597 325 217
56
BanII G_rGCy'C
Cuts at: 1391 2483 4695 4769 1391
Size: 1092 2212 74 4072
Fragments arranged by size:
4072 2212 1092 74
BbeI G_GCGC'C
Cuts at: 3121 3121
Size: 7450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3164 3925 4631 928
Size: 971 1265 761 706 3747
Fragments arranged by size:
3747 1265 971 761 706
BbvCI CC'TCA_GC
Cuts at: 3214 3278 3214
Size: 64 7386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2038 2072 2131 2165 5641 5675 1903
Size: 34 101 34 59 34 3476 34 3678
Fragments arranged by size:
3678 3476 101 59 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2728 5342 6552 2728
Size: 2614 1210 3626
Fragments arranged by size:
3626 2614 1210
BclI T'GATC_A
Cuts at: 1548 3517 4360 1548
Size: 1969 843 4638
Fragments arranged by size:
4638 1969 843
BfrBI ATG'CAT
Cuts at: 3608 3608
Size: 7450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6061 136
Size: 107 122 71 631 605 4389 1525
Fragments arranged by size:
4389 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7445 7445
Size: 7450
BlpI GC'TnA_GC
Cuts at: 2315 4867 2315
Size: 2552 4898
Bme1580I G_kGCm'C
Cuts at: 1790 2467 2530 4333 5511 6667 1790
Size: 677 63 1803 1178 1156 2573
Fragments arranged by size:
2573 1803 1178 1156 677 63
BmgBI CAC'GTC
Cuts at: 1318 3889 1318
Size: 2571 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 2685 5181
Size: 416 53 83 399 24 1044 224 2496
Cuts at: 5181 6139 442
Size: 958 1753
Fragments arranged by size:
2496 1753 1044 958 416 399 224 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7450
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 7450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2571 3894 3920 4045 4309 6110 1239
Size: 1332 1323 26 125 264 1801 2579
Fragments arranged by size:
2579 1801 1332 1323 264 125 26
Bpu10I CC'TnA_GC
Cuts at: 2379 2444 3214 3278 2379
Size: 65 770 64 6551
Fragments arranged by size:
6551 770 65 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3359 4371 5575 6440 6738 3116
Size: 243 1012 1204 865 298 3828
Fragments arranged by size:
3828 1204 1012 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3237 3264 3635 3910 6113 882
Size: 617 1738 27 371 275 2203 2219
Fragments arranged by size:
2219 2203 1738 617 371 275 27
BsaAI yAC'GTr
Cuts at: 493 1204 4743 4747 5241 493
Size: 711 3539 4 494 2702
Fragments arranged by size:
3539 2702 711 494 4
BsaBI GATnn'nnATC
Cuts at: 4254 7444 4254
Size: 3190 4260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3118 5257 5639 6274
Size: 53 83 186 936 1585 2139 382 635
Cuts at: 6274 275
Size: 1451
Fragments arranged by size:
2139 1585 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5883 6281 6555 6702 7113 5883
Size: 398 274 147 411 6220
Fragments arranged by size:
6220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2544 2574 4307 4337 1178
Size: 30 165 30 1141 30 1733 30 4291
Fragments arranged by size:
4291 1733 1141 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 2968 4517 1186
Size: 1366 416 1549 4119
Fragments arranged by size:
4119 1549 1366 416
BseYI C'CCAG_C
Cuts at: 2109 6653 7149 2109
Size: 4544 496 2410
Fragments arranged by size:
4544 2410 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 4109 4254 4616 1163
Size: 573 1551 822 145 362 3997
Fragments arranged by size:
3997 1551 822 573 362 145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4900 5661 6689 1540
Size: 275 186 2899 761 1028 2301
Fragments arranged by size:
2899 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2483 4556 5511 5596 6667 2483
Size: 2073 955 85 1071 3266
Fragments arranged by size:
3266 2073 1071 955 85
BsiWI C'GTAC_G
Cuts at: 4743 4743
Size: 7450
BsmI GAATG_Cn'
Cuts at: 4387 4961 5054 4387
Size: 574 93 6783
Fragments arranged by size:
6783 574 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4420 4420
Size: 7450
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2467 2483 2530 4333 4556 4695 4769
Size: 399 677 16 47 1803 223 139 74
Cuts at: 4769 5511 5596 6667 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1803 1071 742 677 399 223 139
85 74 47 16
BspEI T'CCGG_A
Cuts at: 7113 7113
Size: 7450
BspHI T'CATG_A
Cuts at: 3514 5339 7069 3514
Size: 1825 1730 3895
Fragments arranged by size:
3895 1825 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3726 4805 844
Size: 2882 1079 3489
Fragments arranged by size:
3489 2882 1079
BsrBI CCG'CTC
Cuts at: 1394 1823 2967 5337 1394
Size: 429 1144 2370 3507
Fragments arranged by size:
3507 2370 1144 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3711 4087 4263 5945 6119 65
Size: 1183 2463 376 176 1682 174 1396
Fragments arranged by size:
2463 1682 1396 1183 376 176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6094 6281 1700
Size: 110 4284 187 2869
Fragments arranged by size:
4284 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7450
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7450
BssSI C'ACGA_G
Cuts at: 3284 4274 5510 6522 3284
Size: 990 1236 1012 4212
Fragments arranged by size:
4212 1236 1012 990
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2234 794
Size: 1440 6010
BstBI TT'CG_AA
Cuts at: 2007 6254 2007
Size: 4247 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7450
BstYI r'GATC_y
Cuts at: 1972 2079 3373 3870 4780 5147 5532 5549 7001
Size: 107 1294 497 910 367 385 17 1452
Cuts at: 7001 7087 7099 7445 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1294 910 497 385 367 346
107 86 17 12
BstZ17I GTA'TAC
Cuts at: 3414 3414
Size: 7450
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5941
Fragments arranged by size:
5941 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6711
Fragments arranged by size:
6711 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 3181 4966 5760 5780 2506
Size: 675 1785 794 20 4176
Fragments arranged by size:
4176 1785 794 675 20
ClaI AT'CG_AT
Cuts at: 1554 3031 3428 1554
Size: 1477 397 5576
Fragments arranged by size:
5576 1477 397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7415
DraI TTT'AAA
Cuts at: 4338 4531 4754 4338
Size: 193 223 7034
Fragments arranged by size:
7034 223 193
DraIII CAC_nnn'GTG
Cuts at: 4279 4279
Size: 7450
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2894 6457 809
Size: 507 1578 3563 1802
Fragments arranged by size:
3563 1802 1578 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4897 5786 7415
Size: 54 1425 50 275 18 3067 889 1629
Cuts at: 7415 8
Size: 43
Fragments arranged by size:
3067 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4897 1537
Size: 275 3085 4090
Fragments arranged by size:
4090 3085 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2215 4437 4494 5380 1353
Size: 862 2222 57 886 3423
Fragments arranged by size:
3423 2222 886 862 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6037 6411 6557 387
Size: 1064 138 4448 374 146 1280
Fragments arranged by size:
4448 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2481 2481
Size: 7450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2571 3176 3894 3919 3920 4045 4309
Size: 393 939 605 718 25 1 125 264
Cuts at: 4309 4361 4639 5513 6110 6897 7165 1239
Size: 52 278 874 597 787 268 1524
Fragments arranged by size:
1524 939 874 787 718 605 597 393
278 268 264 125 52 25 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 7450
EcoO109I rG'GnC_Cy
Cuts at: 2329 3469 2329
Size: 1140 6310
EcoRI G'AATT_C
Cuts at: 3819 4759 5137 3819
Size: 940 378 6132
Fragments arranged by size:
6132 940 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2895 3700 2895
Size: 805 6645
FspI TGC'GCA
Cuts at: 5956 5956
Size: 7450
HaeII r_GCGC'y
Cuts at: 1678 3121 6597 1678
Size: 1443 3476 2531
Fragments arranged by size:
3476 2531 1443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2387 2419 3124
Size: 32 135 32 455 32 328 32 705
Cuts at: 3124 3156 3439 3471 3784 3816 3915 3947 4307
Size: 32 283 32 313 32 99 32 360
Cuts at: 4307 4339 6091 6123 6165 6197 1373
Size: 32 1752 32 42 32 2626
Fragments arranged by size:
2626 1752 705 455 360 328 313 283
135 99 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3955 4794 5040 669
Size: 988 281 2017 839 246 3079
Fragments arranged by size:
3079 2017 988 839 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7450
HpaI GTT'AAC
Cuts at: 5040 5040
Size: 7450
KasI G'GCGC_C
Cuts at: 3117 3117
Size: 7450
KpnI G_GTAC'C
Cuts at: 3718 4719 4775 3718
Size: 1001 56 6393
Fragments arranged by size:
6393 1001 56
MfeI C'AATT_G
Cuts at: 3963 5049 3963
Size: 1086 6364
MluI A'CGCG_T
Cuts at: 7326 7326
Size: 7450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3401 3639 6564 6748 1305
Size: 2096 238 2925 184 2007
Fragments arranged by size:
2925 2096 2007 238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5628
Fragments arranged by size:
5628 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 5408 5767 5926 518
Size: 648 1656 2586 359 159 2042
Fragments arranged by size:
2586 2042 1656 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3218 3318 3833 4305 4866 5543 6691 6936
Size: 1612 100 515 472 561 677 1148 245
Cuts at: 6936 1606
Size: 2120
Fragments arranged by size:
2120 1612 1148 677 561 515 472 245
100
NarI GG'CG_CC
Cuts at: 3118 3118
Size: 7450
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5941
Fragments arranged by size:
5941 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7450
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7450
NotI GC'GGCC_GC
Cuts at: 4897 4897
Size: 7450
NsiI A_TGCA'T
Cuts at: 3610 3610
Size: 7450
NspI r_CATG'y
Cuts at: 1132 1229 3256 3700 4078 4105 4808 6353 1132
Size: 97 2027 444 378 27 703 1545 2229
Fragments arranged by size:
2229 2027 1545 703 444 378 97 27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7450
PciI A'CATG_T
Cuts at: 4074 6349 4074
Size: 2275 5175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4252 1108
Size: 3144 4306
PfoI T'CCnGG_A
Cuts at: 2587 2720 2587
Size: 133 7317
PmeI GTTT'AAAC
Cuts at: 4754 4754
Size: 7450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5519 5551 7032 7064 5519
Size: 32 1481 32 5905
Fragments arranged by size:
5905 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 3469 2329
Size: 1140 6310
PshAI GACnn'nnGTC
Cuts at: 1613 4635 1613
Size: 3022 4428
PsiI TTA'TAA
Cuts at: 3743 5020 3743
Size: 1277 6173
PstI C_TGCA'G
Cuts at: 830 2064 2078 3562 3583 4802 830
Size: 1234 14 1484 21 1219 3478
Fragments arranged by size:
3478 1484 1234 1219 21 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7450
PvuII CAG'CTG
Cuts at: 3218 3218
Size: 7450
SacI G_AGCT'C
Cuts at: 2483 2483
Size: 7450
SalI G'TCGA_C
Cuts at: 1936 4792 1936
Size: 2856 4594
SbfI CC_TGCA'GG
Cuts at: 4802 4802
Size: 7450
ScaI AGT'ACT
Cuts at: 5698 7410 5698
Size: 1712 5738
SfcI C'TryA_G
Cuts at: 826 1045 2060 2074 3558 3579 4798 5933 6614
Size: 219 1015 14 1484 21 1219 1135 681
Cuts at: 6614 6805 826
Size: 191 1471
Fragments arranged by size:
1484 1471 1219 1135 1015 681 219 191
21 14
SfoI GGC'GCC
Cuts at: 3119 3119
Size: 7450
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7450
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7450
SmaI CCC'GGG
Cuts at: 1872 4777 1872
Size: 2905 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3095 3338 3981 4386 5554 6455
Size: 197 926 1152 243 643 405 1168 901
Cuts at: 6455 6717 820
Size: 262 1553
Fragments arranged by size:
1553 1168 1152 926 901 643 405 262
243 197
SnaBI TAC'GTA
Cuts at: 493 4747 5241 493
Size: 4254 494 2702
Fragments arranged by size:
4254 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7450
SphI G_CATG'C
Cuts at: 1132 4808 1132
Size: 3676 3774
SspI AAT'ATT
Cuts at: 5 52 4569 5374 5
Size: 47 4517 805 2081
Fragments arranged by size:
4517 2081 805 47
StuI AGG'CCT
Cuts at: 1517 2384 2428 1517
Size: 867 44 6539
Fragments arranged by size:
6539 867 44
StyI C'CwwG_G
Cuts at: 513 1065 2022 3187 4889 513
Size: 552 957 1165 1702 3074
Fragments arranged by size:
3074 1702 1165 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5477 5494 5647 5832 6277 325
Size: 1255 3897 17 153 185 445 1498
Fragments arranged by size:
3897 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3678 5696 7408
Size: 274 80 33 51 1443 1701 2018 1712
Cuts at: 7408 96
Size: 138
Fragments arranged by size:
2018 1712 1701 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2544 2576 3187 3219 7410 7442 2544
Size: 32 611 32 4191 32 2552
Fragments arranged by size:
4191 2552 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2716 3553 1316
Size: 1400 837 5213
Fragments arranged by size:
5213 1400 837
XbaI T'CTAG_A
Cuts at: 3823 4786 3823
Size: 963 6487
XhoI C'TCGA_G
Cuts at: 1943 3981 1943
Size: 2038 5412
XmaI C'CCGG_G
Cuts at: 1870 4775 1870
Size: 2905 4545
XmnI GAAnn'nnTTC
Cuts at: 5579 5579
Size: 7450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5258 276
Size: 53 83 186 936 3724 2468
Fragments arranged by size:
3724 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BstXI Bsu36I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI