Restriction map for
pFN21ASDB0057
(Circular) (Six-base) MAPSORT of: pFN21ASDB0057.seq Check: 2776 from: 1 to: 7108
pFN21ASDB0057 7108 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4918 278
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 4451 1937
Size: 610 1904 4594
Fragments arranged by size:
4594 1904 610
Acc65I G'GTAC_C
Cuts at: 3282 4429 3282
Size: 1147 5961
AclI AA'CG_TT
Cuts at: 1563 5235 5608 1563
Size: 3672 373 3063
Fragments arranged by size:
3672 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 3665 5171 6555 6823 1632
Size: 1580 453 1506 1384 268 1917
Fragments arranged by size:
1917 1580 1506 1384 453 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6911
AflIII A'CryG_T
Cuts at: 1203 6007 6984 1203
Size: 4804 977 1327
Fragments arranged by size:
4804 1327 977
AgeI A'CCGG_T
Cuts at: 5939 5939
Size: 7108
AhdI GACnn_n'nnGTC
Cuts at: 5837 5837
Size: 7108
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3675 1184
Size: 2491 4617
AlwNI CAG_nnn'CTG
Cuts at: 2119 2440 3487 3868 4012 4308 4851 6423 2119
Size: 321 1047 381 144 296 543 1572 2804
Fragments arranged by size:
2804 1572 1047 543 381 321 296 144
ApaI G_GGCC'C
Cuts at: 3296 3296
Size: 7108
ApaLI G'TGCA_C
Cuts at: 5165 6321 5165
Size: 1156 5952
ApoI r'AATT_y
Cuts at: 1493 3167 4196 4417 4643 4795 6904 1493
Size: 1674 1029 221 226 152 2109 1697
Fragments arranged by size:
2109 1697 1674 1029 226 221 152
AseI AT'TA_AT
Cuts at: 160 1032 5662 160
Size: 872 4630 1606
Fragments arranged by size:
4630 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 2363 2534 2625 2827 3129 4098
Size: 73 230 190 171 91 202 302 969
Cuts at: 4098 4433 1870
Size: 335 4545
Fragments arranged by size:
4545 969 335 302 230 202 190 171
91 73
BamHI G'GATC_C
Cuts at: 4438 4805 4438
Size: 367 6741
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 3282 3737 4015 4311
Size: 325 217 627 283 1212 455 278 296
Cuts at: 4311 4429 5884 618
Size: 118 1455 1842
Fragments arranged by size:
1842 1455 1212 627 455 325 296 283
278 217 118
BanII G_rGCy'C
Cuts at: 1391 2364 3296 3732 4427 1391
Size: 973 932 436 695 4072
Fragments arranged by size:
4072 973 932 695 436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6137
BbvCI CC'TCA_GC
Cuts at: 2081 4323 2081
Size: 2242 4866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3656 3690 5299 5333 1903
Size: 34 1719 34 1609 34 3678
Fragments arranged by size:
3678 1719 1609 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5000 6210 5000
Size: 1210 5898
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7108
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2319 3314
Size: 107 122 71 631 605 376 271 995
Cuts at: 3314 4358 5719 136
Size: 1044 1361 1525
Fragments arranged by size:
1525 1361 1044 995 631 605 376 271
122 107 71
BglII A'GATC_T
Cuts at: 2463 7103 2463
Size: 4640 2468
BlpI GC'TnA_GC
Cuts at: 4525 4525
Size: 7108
Bme1580I G_kGCm'C
Cuts at: 1790 2324 3296 3740 5169 6325 1790
Size: 534 972 444 1429 1156 2573
Fragments arranged by size:
2573 1429 1156 972 534 444
BmgBI CAC'GTC
Cuts at: 1318 2131 4284 1318
Size: 813 2153 4142
Fragments arranged by size:
4142 2153 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 3056 3290
Size: 416 53 83 399 24 730 909 234
Cuts at: 3290 4839 5797 442
Size: 1549 958 1753
Fragments arranged by size:
1753 1549 958 909 730 416 399 234
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2677 3097 2507
Size: 170 420 6518
Fragments arranged by size:
6518 420 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2984 3224 3389 3446 3512 3533 3890
Size: 1025 720 240 165 57 66 21 357
Cuts at: 3890 4161 4178 5768 1239
Size: 271 17 1590 2579
Fragments arranged by size:
2579 1590 1025 720 357 271 240 165
66 57 21 17
Bpu10I CC'TnA_GC
Cuts at: 2081 4323 2081
Size: 2242 4866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 3770 3887 3893 5233 6098 6396 2039
Size: 1731 117 6 1340 865 298 2751
Fragments arranged by size:
2751 1731 1340 865 298 117 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2478 5771 882
Size: 617 979 3293 2219
Fragments arranged by size:
3293 2219 979 617
BsaAI yAC'GTr
Cuts at: 493 1204 4405 4899 493
Size: 711 3201 494 2702
Fragments arranged by size:
3201 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7102 7102
Size: 7108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 2544 4026 4915
Size: 53 83 186 936 672 339 1482 889
Cuts at: 4915 5297 5932 275
Size: 382 635 1451
Fragments arranged by size:
1482 1451 936 889 672 635 382 339
186 83 53
BsaWI w'CCGG_w
Cuts at: 5541 5939 6213 6360 6771 5541
Size: 398 274 147 411 5878
Fragments arranged by size:
5878 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 2549 2579 2963
Size: 30 165 30 1074 30 42 30 384
Cuts at: 2963 2993 3980 4010 1178
Size: 30 987 30 4276
Fragments arranged by size:
4276 1074 987 384 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2554 3191 3338 3443 3446 3488 3509
Size: 922 446 637 147 105 3 42 21
Cuts at: 3509 3575 3731 3887 3959 4001 4004 1186
Size: 66 156 156 72 42 3 4290
Fragments arranged by size:
4290 922 637 446 156 156 147 105
72 66 42 42 21 3 3
BseYI C'CCAG_C
Cuts at: 3198 3220 3357 6311 6807 3198
Size: 22 137 2954 496 3499
Fragments arranged by size:
3499 2954 496 137 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2719 1163
Size: 573 983 5552
Fragments arranged by size:
5552 983 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4558 5319 6347 1540
Size: 275 186 2557 761 1028 2301
Fragments arranged by size:
2557 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2280 2400 2960 3732 5169 5254 6325 2280
Size: 120 560 772 1437 85 1071 3063
Fragments arranged by size:
3063 1437 1071 772 560 120 85
BsmI GAATG_Cn'
Cuts at: 2392 3025 4619 4712 2392
Size: 633 1594 93 4788
Fragments arranged by size:
4788 1594 633 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2324 2364 2400 2960 3296 3732
Size: 399 490 44 40 36 560 336 436
Cuts at: 3732 3740 4427 5169 5254 6325 1391
Size: 8 687 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 560 490 436 399
336 85 44 40 36 8
BspEI T'CCGG_A
Cuts at: 6771 6771
Size: 7108
BspHI T'CATG_A
Cuts at: 3960 4997 6727 3960
Size: 1037 1730 4341
Fragments arranged by size:
4341 1730 1037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2496 3411 3528 4463 844
Size: 1652 915 117 935 3489
Fragments arranged by size:
3489 1652 935 915 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2421 2667 3421 3682 4995 1394
Size: 429 598 246 754 261 1313 3507
Fragments arranged by size:
3507 1313 754 598 429 261 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 4119 5603 5777 65
Size: 1183 2871 1484 174 1396
Fragments arranged by size:
2871 1484 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3966 4022 5752 5939 1700
Size: 110 2156 56 1730 187 2869
Fragments arranged by size:
2869 2156 1730 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7108
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7108
BssSI C'ACGA_G
Cuts at: 2108 2753 4224 5168 6180 2108
Size: 645 1471 944 1012 3036
Fragments arranged by size:
3036 1471 1012 944 645
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3487 794
Size: 2693 4415
BstBI TT'CG_AA
Cuts at: 2007 5912 2007
Size: 3905 3203
BstEII G'GTnAC_C
Cuts at: 1184 2146 1184
Size: 962 6146
BstXI CCAn_nnnn'nTGG
Cuts at: 4114 4114
Size: 7108
BstYI r'GATC_y
Cuts at: 1972 2115 2463 3825 4304 4438 4805 5190 5207
Size: 143 348 1362 479 134 367 385 17
Cuts at: 5207 6659 6745 6757 7103 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1362 479 385 367 348 346
143 134 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2883 3288 2883
Size: 405 6703
BtgI C'CryG_G
Cuts at: 513 1065 2022 2124 3570 4107 513
Size: 552 957 102 1446 537 3514
Fragments arranged by size:
3514 1446 957 552 537 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6369
Fragments arranged by size:
6369 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3155 4001 4071 4624 5418 5438 2860
Size: 295 846 70 553 794 20 4530
Fragments arranged by size:
4530 846 794 553 295 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7073
DraI TTT'AAA
Cuts at: 4412 4412
Size: 7108
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6115 809
Size: 507 4799 1802
Fragments arranged by size:
4799 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2311 2719
Size: 54 1425 50 275 18 195 286 408
Cuts at: 2719 3555 4052 4555 5444 7073 8
Size: 836 497 503 889 1629 43
Fragments arranged by size:
1629 1425 889 836 503 497 408 286
275 195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4555 1537
Size: 275 2743 4090
Fragments arranged by size:
4090 2743 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3203 3236 3315 3641 5038 1353
Size: 1116 734 33 79 326 1397 3423
Fragments arranged by size:
3423 1397 1116 734 326 79 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3259 3836 4406 5695 6069 6215
Size: 1064 138 1670 577 570 1289 374 146
Cuts at: 6215 387
Size: 1280
Fragments arranged by size:
1670 1289 1280 1064 577 570 374 146
138
EcoICRI GAG'CTC
Cuts at: 3730 3730
Size: 7108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2984 3212 3224 3389 3446 3512
Size: 393 632 720 228 12 165 57 66
Cuts at: 3512 3533 3665 3890 4161 4178 5171 5768 6555
Size: 21 132 225 271 17 993 597 787
Cuts at: 6555 6823 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 720 632 597 393 271
268 228 225 165 132 66 57 21
17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3755 3872 3755
Size: 117 6991
EcoO109I rG'GnC_Cy
Cuts at: 2050 2259 2349 2557 2792 3142 3292 3517 3865
Size: 209 90 208 235 350 150 225 348
Cuts at: 3865 4119 2050
Size: 254 5039
Fragments arranged by size:
5039 350 348 254 235 225 209 208
150 90
EcoRI G'AATT_C
Cuts at: 3167 4196 4417 4795 3167
Size: 1029 221 378 5480
Fragments arranged by size:
5480 1029 378 221
FspI TGC'GCA
Cuts at: 5614 5614
Size: 7108
HaeII r_GCGC'y
Cuts at: 1678 6255 1678
Size: 4577 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2477 2509 2549
Size: 32 135 32 635 32 238 32 40
Cuts at: 2549 2581 5749 5781 5823 5855 1373
Size: 32 3168 32 42 32 2626
Fragments arranged by size:
3168 2626 635 238 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4452 4698 669
Size: 988 281 2514 246 3079
Fragments arranged by size:
3079 2514 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7108
HpaI GTT'AAC
Cuts at: 4698 4698
Size: 7108
KpnI G_GTAC'C
Cuts at: 3286 4433 3286
Size: 1147 5961
MfeI C'AATT_G
Cuts at: 4707 4707
Size: 7108
MluI A'CGCG_T
Cuts at: 6984 6984
Size: 7108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3618 4143 6222 6406 1305
Size: 2313 525 2079 184 2007
Fragments arranged by size:
2313 2079 2007 525 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2313 2721 10
Size: 54 1425 343 195 286 408 4397
Fragments arranged by size:
4397 1425 408 343 286 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2645 2649 2690 2735 4112 5066
Size: 648 963 516 4 41 45 1377 954
Cuts at: 5066 5425 5584 518
Size: 359 159 2042
Fragments arranged by size:
2042 1377 963 954 648 516 359 159
45 41 4
MspA1I CmG'CkG
Cuts at: 1606 2804 3202 4181 4327 4524 5201 6349 6594
Size: 1198 398 979 146 197 677 1148 245
Cuts at: 6594 1606
Size: 2120
Fragments arranged by size:
2120 1198 1148 979 677 398 245 197
146
NaeI GCC'GGC
Cuts at: 4024 4024
Size: 7108
NcoI C'CATG_G
Cuts at: 513 1065 2022 2124 3570 4107 513
Size: 552 957 102 1446 537 3514
Fragments arranged by size:
3514 1446 957 552 537 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7108
NgoMIV G'CCGG_C
Cuts at: 4022 4022
Size: 7108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7108
NotI GC'GGCC_GC
Cuts at: 4555 4555
Size: 7108
NspI r_CATG'y
Cuts at: 1132 1229 2603 4352 4466 6011 1132
Size: 97 1374 1749 114 1545 2229
Fragments arranged by size:
2229 1749 1545 1374 114 97
PasI CC'CwG_GG
Cuts at: 1348 2920 3010 1348
Size: 1572 90 5446
Fragments arranged by size:
5446 1572 90
PciI A'CATG_T
Cuts at: 6007 6007
Size: 7108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4012 4308 1108
Size: 2904 296 3908
Fragments arranged by size:
3908 2904 296
PfoI T'CCnGG_A
Cuts at: 3000 3366 3447 3708 3828 3975 3000
Size: 366 81 261 120 147 6133
Fragments arranged by size:
6133 366 261 147 120 81
PmeI GTTT'AAAC
Cuts at: 4412 4412
Size: 7108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3891 3923 5177 5209 6690 6722 3891
Size: 32 1254 32 1481 32 4277
Fragments arranged by size:
4277 1481 1254 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2349 2557 3142 3517 3865 4119 2349
Size: 208 585 375 348 254 5338
Fragments arranged by size:
5338 585 375 348 254 208
PshAI GACnn'nnGTC
Cuts at: 1613 2543 1613
Size: 930 6178
PsiI TTA'TAA
Cuts at: 4678 4678
Size: 7108
PspOMI G'GGCC_C
Cuts at: 3292 3292
Size: 7108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2420 2452 2420
Size: 32 7076
PstI C_TGCA'G
Cuts at: 830 3408 3756 3810 4066 4460 830
Size: 2578 348 54 256 394 3478
Fragments arranged by size:
3478 2578 394 348 256 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7108
PvuII CAG'CTG
Cuts at: 3202 4181 3202
Size: 979 6129
SacI G_AGCT'C
Cuts at: 3732 3732
Size: 7108
SalI G'TCGA_C
Cuts at: 1936 4450 1936
Size: 2514 4594
SanDI GG'GwC_CC
Cuts at: 4119 4119
Size: 7108
SapI GCTCTTCn'nnn_
Cuts at: 3236 3236
Size: 7108
SbfI CC_TGCA'GG
Cuts at: 3408 4460 3408
Size: 1052 6056
ScaI AGT'ACT
Cuts at: 5356 7068 5356
Size: 1712 5396
SexAI A'CCwGG_T
Cuts at: 2439 2439
Size: 7108
SfcI C'TryA_G
Cuts at: 826 1045 3404 3752 3806 4062 4456 5591 6272
Size: 219 2359 348 54 256 394 1135 681
Cuts at: 6272 6463 826
Size: 191 1471
Fragments arranged by size:
2359 1471 1135 681 394 348 256 219
191 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2048
Size: 7108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7108
SmaI CCC'GGG
Cuts at: 1872 2175 2627 3131 4435 1872
Size: 303 452 504 1304 4545
Fragments arranged by size:
4545 1304 504 452 303
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 3785 3902 3908 5212 6113
Size: 197 926 111 1731 117 6 1304 901
Cuts at: 6113 6375 820
Size: 262 1553
Fragments arranged by size:
1731 1553 1304 926 901 262 197 117
111 6
SnaBI TAC'GTA
Cuts at: 493 4405 4899 493
Size: 3912 494 2702
Fragments arranged by size:
3912 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7108
SphI G_CATG'C
Cuts at: 1132 4466 1132
Size: 3334 3774
SspI AAT'ATT
Cuts at: 5 52 5032 5
Size: 47 4980 2081
Fragments arranged by size:
4980 2081 47
StuI AGG'CCT
Cuts at: 1517 2881 3308 4081 1517
Size: 1364 427 773 4544
Fragments arranged by size:
4544 1364 773 427
StyI C'CwwG_G
Cuts at: 513 1065 2022 2037 2124 2496 3147 3378 3570
Size: 552 957 15 87 372 651 231 192
Cuts at: 3570 4107 4547 513
Size: 537 440 3074
Fragments arranged by size:
3074 957 651 552 537 440 372 231
192 87 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2423 2657 2975 3301 5135 5152
Size: 1255 764 79 234 318 326 1834 17
Cuts at: 5152 5305 5490 5935 325
Size: 153 185 445 1498
Fragments arranged by size:
1834 1498 1255 764 445 326 318 234
185 153 79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3561 5354 7066
Size: 274 80 33 51 1443 1584 1793 1712
Cuts at: 7066 96
Size: 138
Fragments arranged by size:
1793 1712 1584 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2950 2982 3980 4012 7068 7100 2950
Size: 32 998 32 3056 32 2958
Fragments arranged by size:
3056 2958 998 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7108
XbaI T'CTAG_A
Cuts at: 4444 4444
Size: 7108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2288 2321 3031 2288
Size: 33 710 6365
Fragments arranged by size:
6365 710 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7108
XmaI C'CCGG_G
Cuts at: 1870 2173 2625 3129 4433 1870
Size: 303 452 504 1304 4545
Fragments arranged by size:
4545 1304 504 452 303
XmnI GAAnn'nnTTC
Cuts at: 2478 5237 2478
Size: 2759 4349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4916 276
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI RsrII
SacII SfoI SrfI SwaI