Restriction map for
pFN21ASDB0055
(Circular) (Six-base) MAPSORT of: pFN21ASDB0055.seq Check: 8942 from: 1 to: 8725
pFN21ASDB0055 8725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2298 3561 6535 278
Size: 53 83 186 936 762 1263 2974 2468
Fragments arranged by size:
2974 2468 1263 936 762 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 4437 5310 5730 6068 1937
Size: 217 2283 873 420 338 4594
Fragments arranged by size:
4594 2283 873 420 338 217
Acc65I G'GTAC_C
Cuts at: 5491 6046 5491
Size: 555 8170
AclI AA'CG_TT
Cuts at: 1563 5431 6852 7225 1563
Size: 3868 1421 373 3063
Fragments arranged by size:
3868 3063 1421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 3019 4665 4967 6788 8172 8440 1632
Size: 1298 89 1646 302 1821 1384 268 1917
Fragments arranged by size:
1917 1821 1646 1384 1298 302 268 89
AflII C'TTAA_G
Cuts at: 820 1017 4793 820
Size: 197 3776 4752
Fragments arranged by size:
4752 3776 197
AflIII A'CryG_T
Cuts at: 1203 5022 5093 7624 8601 1203
Size: 3819 71 2531 977 1327
Fragments arranged by size:
3819 2531 1327 977 71
AgeI A'CCGG_T
Cuts at: 7556 7556
Size: 8725
AhdI GACnn_n'nnGTC
Cuts at: 7454 7454
Size: 8725
AleI CACnn'nnGTG
Cuts at: 1166 4121 1166
Size: 2955 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2598 4267 5229 1184
Size: 1414 1669 962 4680
Fragments arranged by size:
4680 1669 1414 962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 3807 3839 2513
Size: 32 1262 32 7399
Fragments arranged by size:
7399 1262 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2288 2419 3042 6468 8040 2288
Size: 131 623 3426 1572 2973
Fragments arranged by size:
3426 2973 1572 623 131
ApaLI G'TGCA_C
Cuts at: 3262 6782 7938 3262
Size: 3520 1156 4049
Fragments arranged by size:
4049 3520 1156
ApoI r'AATT_y
Cuts at: 1493 5838 5863 6034 6260 6412 8521 1493
Size: 4345 25 171 226 152 2109 1697
Fragments arranged by size:
4345 2109 1697 226 171 152 25
AseI AT'TA_AT
Cuts at: 160 1032 7279 160
Size: 872 6247 1606
Fragments arranged by size:
6247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
AvaI C'yCGr_G
Cuts at: 1870 1943 6050 1870
Size: 73 4107 4545
Fragments arranged by size:
4545 4107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4958 4991 4958
Size: 33 8692
BamHI G'GATC_C
Cuts at: 4450 4759 6055 6422 4450
Size: 309 1296 367 6753
Fragments arranged by size:
6753 1296 367 309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4709 5060 5491 6046 7501
Size: 325 217 627 2922 351 431 555 1455
Cuts at: 7501 618
Size: 1842
Fragments arranged by size:
2922 1842 1455 627 555 431 351 325
217
BanII G_rGCy'C
Cuts at: 1391 2677 4192 5043 5454 6044 1391
Size: 1286 1515 851 411 590 4072
Fragments arranged by size:
4072 1515 1286 851 590 411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2946 3223 3553 3561 4409 4565 5939
Size: 971 1047 277 330 8 848 156 1374
Cuts at: 5939 928
Size: 3714
Fragments arranged by size:
3714 1374 1047 971 848 330 277 156
8
BbvCI CC'TCA_GC
Cuts at: 4871 4871
Size: 8725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6916 6950 1903
Size: 34 4979 34 3678
Fragments arranged by size:
4979 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6617 7827 6617
Size: 1210 7515
BclI T'GATC_A
Cuts at: 1548 4291 4696 1548
Size: 2743 405 5577
Fragments arranged by size:
5577 2743 405
BfrBI ATG'CAT
Cuts at: 2575 4254 2575
Size: 1679 7046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5038 5471 7336
Size: 107 122 71 631 605 3366 433 1865
Cuts at: 7336 136
Size: 1525
Fragments arranged by size:
3366 1865 1525 631 605 433 122 107
71
BglII A'GATC_T
Cuts at: 8720 8720
Size: 8725
BlpI GC'TnA_GC
Cuts at: 5958 6005 6142 5958
Size: 47 137 8541
Fragments arranged by size:
8541 137 47
Bme1580I G_kGCm'C
Cuts at: 1790 3266 4128 5065 5242 6786 7942 1790
Size: 1476 862 937 177 1544 1156 2573
Fragments arranged by size:
2573 1544 1476 1156 937 862 177
BmgBI CAC'GTC
Cuts at: 1318 3948 1318
Size: 2630 6095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2750 3425 5989
Size: 416 53 83 399 24 1333 675 2564
Cuts at: 5989 6456 7414 442
Size: 467 958 1753
Fragments arranged by size:
2564 1753 1333 958 675 467 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2310 2348 2474 5566 5681 5699 7385
Size: 833 238 38 126 3092 115 18 1686
Cuts at: 7385 1239
Size: 2579
Fragments arranged by size:
3092 2579 1686 833 238 126 115 38
18
Bpu10I CC'TnA_GC
Cuts at: 4052 4871 4052
Size: 819 7906
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3002 5438 6850 7715 8013 2149
Size: 853 2436 1412 865 298 2861
Fragments arranged by size:
2861 2436 1412 865 853 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7388 882
Size: 617 5889 2219
Fragments arranged by size:
5889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6022 6516 493
Size: 711 4818 494 2702
Fragments arranged by size:
4818 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8719 8719
Size: 8725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 3558 6532 6914
Size: 53 83 186 936 762 1263 2974 382
Cuts at: 6914 7549 275
Size: 635 1451
Fragments arranged by size:
2974 1451 1263 936 762 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3156 7158 7556 7830 7977 8388 3156
Size: 4002 398 274 147 411 3493
Fragments arranged by size:
4002 3493 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2513 2543 5009
Size: 30 165 30 852 30 228 30 2466
Cuts at: 5009 5039 1178
Size: 30 4864
Fragments arranged by size:
4864 2466 852 228 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3450 1186
Size: 2264 6461
BseYI C'CCAG_C
Cuts at: 5130 5466 7928 8424 5130
Size: 336 2462 496 5431
Fragments arranged by size:
5431 2462 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4401 5720 1163
Size: 573 2665 1319 4168
Fragments arranged by size:
4168 2665 1319 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6175 6936 7964 1540
Size: 275 186 4174 761 1028 2301
Fragments arranged by size:
4174 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 3266 5007 5022 5265 5454 6786 6871 7942
Size: 1142 1741 15 243 189 1332 85 1071
Cuts at: 7942 2124
Size: 2907
Fragments arranged by size:
2907 1741 1332 1142 1071 243 189 85
15
BsmI GAATG_Cn'
Cuts at: 2911 4256 4907 6236 6329 2911
Size: 1345 651 1329 93 5307
Fragments arranged by size:
5307 1345 1329 651 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2286 2286
Size: 8725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2677 3266 4128 4192 5007 5022
Size: 399 334 553 589 862 64 815 15
Cuts at: 5022 5043 5065 5242 5265 5454 6044 6786 6871
Size: 21 22 177 23 189 590 742 85
Cuts at: 6871 7942 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 862 815 742 590 589 553
399 334 189 177 85 64 23 22
21 15
BspEI T'CCGG_A
Cuts at: 8388 8388
Size: 8725
BspHI T'CATG_A
Cuts at: 6614 8344 6614
Size: 1730 6995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4007 5465 6080 844
Size: 3163 1458 615 3489
Fragments arranged by size:
3489 3163 1458 615
BsrBI CCG'CTC
Cuts at: 1394 1823 5626 6612 1394
Size: 429 3803 986 3507
Fragments arranged by size:
3803 3507 986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4989 7220 7394 65
Size: 1183 3741 2231 174 1396
Fragments arranged by size:
3741 2231 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3093 4046 7369 7556 1700
Size: 110 1283 953 3323 187 2869
Fragments arranged by size:
3323 2869 1283 953 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8725
BssSI C'ACGA_G
Cuts at: 4598 5066 6785 7797 4598
Size: 468 1719 1012 5526
Fragments arranged by size:
5526 1719 1012 468
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5011 794
Size: 4217 4508
BstBI TT'CG_AA
Cuts at: 2007 7529 2007
Size: 5522 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8725
BstXI CCAn_nnnn'nTGG
Cuts at: 5227 5841 5227
Size: 614 8111
BstYI r'GATC_y
Cuts at: 1972 2463 4450 4759 6055 6422 6807 6824 8276
Size: 491 1987 309 1296 367 385 17 1452
Cuts at: 8276 8362 8374 8720 1972
Size: 86 12 346 1977
Fragments arranged by size:
1987 1977 1452 1296 491 385 367 346
309 86 17 12
BstZ17I GTA'TAC
Cuts at: 5311 5731 5311
Size: 420 8305
Bsu36I CC'TnA_GG
Cuts at: 2767 4016 4302 2767
Size: 1249 286 7190
Fragments arranged by size:
7190 1249 286
BtgI C'CryG_G
Cuts at: 513 1065 2533 3797 4072 5220 5497 513
Size: 552 1468 1264 275 1148 277 3741
Fragments arranged by size:
3741 1468 1264 1148 552 277 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4497 487
Size: 683 56 3271 4715
Fragments arranged by size:
4715 3271 683 56
BtsI GCAGTG_nn'
Cuts at: 4438 5539 5554 6241 7035 7055 4438
Size: 1101 15 687 794 20 6108
Fragments arranged by size:
6108 1101 794 687 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8690
DraI TTT'AAA
Cuts at: 3442 4033 4237 5742 6029 3442
Size: 591 204 1505 287 6138
Fragments arranged by size:
6138 1505 591 287 204
DraIII CAC_nnn'GTG
Cuts at: 3292 5690 3292
Size: 2398 6327
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7732 809
Size: 507 6416 1802
Fragments arranged by size:
6416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 2277 3748
Size: 54 1425 50 275 18 397 50 1471
Cuts at: 3748 3858 4864 5217 6172 7061 8690 8
Size: 110 1006 353 955 889 1629 43
Fragments arranged by size:
1629 1471 1425 1006 955 889 397 353
275 110 54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6172 1537
Size: 275 4360 4090
Fragments arranged by size:
4360 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3044 3636 3867 4641 5226 5522 6655 1353
Size: 1691 592 231 774 585 296 1133 3423
Fragments arranged by size:
3423 1691 1133 774 592 585 296 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3240 7312 7686 7832 387
Size: 1064 138 1651 4072 374 146 1280
Fragments arranged by size:
4072 1651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5452 5452
Size: 8725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2310 2348 2474 2930 3019 4665
Size: 393 440 238 38 126 456 89 1646
Cuts at: 4665 4967 5566 5681 5699 6788 7385 8172 8440
Size: 302 599 115 18 1089 597 787 268
Cuts at: 8440 1239
Size: 1524
Fragments arranged by size:
1646 1524 1089 787 599 597 456 440
393 302 268 238 126 115 89 38
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2733 5012 5678 2733
Size: 2279 666 5780
Fragments arranged by size:
5780 2279 666
EcoO109I rG'GnC_Cy
Cuts at: 3076 5665 5885 3076
Size: 2589 220 5916
Fragments arranged by size:
5916 2589 220
EcoRI G'AATT_C
Cuts at: 5863 6034 6412 5863
Size: 171 378 8176
Fragments arranged by size:
8176 378 171
EcoRV GAT'ATC
Cuts at: 4984 5589 4984
Size: 605 8120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3467 4790 5767 3467
Size: 1323 977 6425
Fragments arranged by size:
6425 1323 977
FseI GG_CCGG'CC
Cuts at: 4050 4050
Size: 8725
FspI TGC'GCA
Cuts at: 7231 7231
Size: 8725
HaeII r_GCGC'y
Cuts at: 1678 7872 1678
Size: 6194 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2249 2281 2359
Size: 32 135 32 575 32 70 32 78
Cuts at: 2359 2391 3049 3081 3468 3500 3920 3952 4045
Size: 32 658 32 387 32 420 32 93
Cuts at: 4045 4077 4258 4276 4290 4308 4498 4530 5504
Size: 32 181 18 14 18 190 32 974
Cuts at: 5504 5536 7366 7398 7440 7472 1373
Size: 32 1830 32 42 32 2626
Fragments arranged by size:
2626 1830 974 658 575 420 387 190
181 135 93 78 70 42 32 32
32 32 32 32 32 32 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2411 2718 3981 6069 6315 669
Size: 988 281 473 307 1263 2088 246 3079
Fragments arranged by size:
3079 2088 1263 988 473 307 281 246
HindIII A'AGCT_T
Cuts at: 748 4139 5295 748
Size: 3391 1156 4178
Fragments arranged by size:
4178 3391 1156
HpaI GTT'AAC
Cuts at: 6315 6315
Size: 8725
KpnI G_GTAC'C
Cuts at: 5495 6050 5495
Size: 555 8170
MfeI C'AATT_G
Cuts at: 6324 6324
Size: 8725
MluI A'CGCG_T
Cuts at: 8601 8601
Size: 8725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2351 2485 2977 4200 7839 8023 1305
Size: 1046 134 492 1223 3639 184 2007
Fragments arranged by size:
3639 2007 1223 1046 492 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 3750 3860 4866 10
Size: 54 1425 343 447 1471 110 1006 3869
Fragments arranged by size:
3869 1471 1425 1006 447 343 110 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 4121 4249 5025 5211 6683 7042
Size: 648 1306 1649 128 776 186 1472 359
Cuts at: 7042 7201 518
Size: 159 2042
Fragments arranged by size:
2042 1649 1472 1306 776 648 359 186
159 128
MspA1I CmG'CkG
Cuts at: 1606 2326 3523 3677 3739 4819 5036 5155 5659
Size: 720 1197 154 62 1080 217 119 504
Cuts at: 5659 6141 6818 7966 8211 1606
Size: 482 677 1148 245 2120
Fragments arranged by size:
2120 1197 1148 1080 720 677 504 482
245 217 154 119 62
NaeI GCC'GGC
Cuts at: 4048 4048
Size: 8725
NcoI C'CATG_G
Cuts at: 513 1065 2533 3797 4072 5220 513
Size: 552 1468 1264 275 1148 4018
Fragments arranged by size:
4018 1468 1264 1148 552 275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8725
NgoMIV G'CCGG_C
Cuts at: 4046 4046
Size: 8725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8725
NotI GC'GGCC_GC
Cuts at: 6172 6172
Size: 8725
NsiI A_TGCA'T
Cuts at: 2577 4256 2577
Size: 1679 7046
NspI r_CATG'y
Cuts at: 1132 1229 3104 5026 5097 6083 7628 1132
Size: 97 1875 1922 71 986 1545 2229
Fragments arranged by size:
2229 1922 1875 1545 986 97 71
PasI CC'CwG_GG
Cuts at: 1348 2592 1348
Size: 1244 7481
PciI A'CATG_T
Cuts at: 5022 5093 7624 5022
Size: 71 2531 6123
Fragments arranged by size:
6123 2531 71
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2219 2389 5158 1108
Size: 1111 170 2769 4675
Fragments arranged by size:
4675 2769 1111 170
PfoI T'CCnGG_A
Cuts at: 2451 5582 5676 2451
Size: 3131 94 5500
Fragments arranged by size:
5500 3131 94
PmeI GTTT'AAAC
Cuts at: 6029 6029
Size: 8725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2513 2545 6794 6826 8307 8339 2513
Size: 32 4249 32 1481 32 2899
Fragments arranged by size:
4249 2899 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3076 5665 5885 3076
Size: 2589 220 5916
Fragments arranged by size:
5916 2589 220
PshAI GACnn'nnGTC
Cuts at: 1613 3903 1613
Size: 2290 6435
PsiI TTA'TAA
Cuts at: 3375 6295 3375
Size: 2920 5805
PstI C_TGCA'G
Cuts at: 830 2309 3622 3676 5814 6077 830
Size: 1479 1313 54 2138 263 3478
Fragments arranged by size:
3478 2138 1479 1313 263 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8725
PvuII CAG'CTG
Cuts at: 2326 3739 5155 2326
Size: 1413 1416 5896
Fragments arranged by size:
5896 1416 1413
SacI G_AGCT'C
Cuts at: 5454 5454
Size: 8725
SalI G'TCGA_C
Cuts at: 1936 6067 1936
Size: 4131 4594
SanDI GG'GwC_CC
Cuts at: 3076 3076
Size: 8725
SapI GCTCTTCn'nnn_
Cuts at: 3044 3636 3044
Size: 592 8133
SbfI CC_TGCA'GG
Cuts at: 6077 6077
Size: 8725
ScaI AGT'ACT
Cuts at: 4892 6973 8685 4892
Size: 2081 1712 4932
Fragments arranged by size:
4932 2081 1712
SfcI C'TryA_G
Cuts at: 826 1045 2305 3405 3618 3672 4827 5810 6073
Size: 219 1260 1100 213 54 1155 983 263
Cuts at: 6073 7208 7889 8080 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1260 1155 1135 1100 983 681 263
219 213 191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8725
SmaI CCC'GGG
Cuts at: 1872 6052 1872
Size: 4180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2164 3017 4793 5453 6829 7730
Size: 197 926 221 853 1776 660 1376 901
Cuts at: 7730 7992 820
Size: 262 1553
Fragments arranged by size:
1776 1553 1376 926 901 853 660 262
221 197
SnaBI TAC'GTA
Cuts at: 493 6022 6516 493
Size: 5529 494 2702
Fragments arranged by size:
5529 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8725
SphI G_CATG'C
Cuts at: 1132 6083 1132
Size: 4951 3774
SspI AAT'ATT
Cuts at: 5 52 4661 6649 5
Size: 47 4609 1988 2081
Fragments arranged by size:
4609 2081 1988 47
StuI AGG'CCT
Cuts at: 1517 3534 4652 5795 1517
Size: 2017 1118 1143 4447
Fragments arranged by size:
4447 2017 1143 1118
StyI C'CwwG_G
Cuts at: 513 1065 2533 3797 3886 4072 4157 5220 6164
Size: 552 1468 1264 89 186 85 1063 944
Cuts at: 6164 513
Size: 3074
Fragments arranged by size:
3074 1468 1264 1063 944 552 186 89
85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3860 3866 6752 6769 6922 7107
Size: 1255 1836 444 6 2886 17 153 185
Cuts at: 7107 7552 325
Size: 445 1498
Fragments arranged by size:
2886 1836 1498 1255 445 444 185 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4582 4594 4890
Size: 274 80 33 51 1443 2605 12 296
Cuts at: 4890 6971 8683 96
Size: 2081 1712 138
Fragments arranged by size:
2605 2081 1712 1443 296 274 138 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2850 2882 3201 3233 8685 8717 2255
Size: 32 563 32 319 32 5452 32 2263
Fragments arranged by size:
5452 2263 563 319 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 3721 4475 1316
Size: 843 1562 754 5566
Fragments arranged by size:
5566 1562 843 754
XbaI T'CTAG_A
Cuts at: 3346 6061 3346
Size: 2715 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2616 2728 2754 5152 2616
Size: 112 26 2398 6189
Fragments arranged by size:
6189 2398 112 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8725
XmaI C'CCGG_G
Cuts at: 1870 6050 1870
Size: 4180 4545
XmnI GAAnn'nnTTC
Cuts at: 2205 2943 4176 4773 6854 2205
Size: 738 1233 597 2081 4076
Fragments arranged by size:
4076 2081 1233 738 597
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2296 3559 6533 276
Size: 53 83 186 936 762 1263 2974 2468
Fragments arranged by size:
2974 2468 1263 936 762 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI
FspAI KasI NarI NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI