Restriction map for
pFN21ASDB0046
(Circular) (Six-base) MAPSORT of: pFN21ASDB0046.seq Check: 5465 from: 1 to: 10654
pFN21ASDB0046 10654 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2812 2812
Size: 10654
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8464 278
Size: 53 83 186 936 6928 2468
Fragments arranged by size:
6928 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7355 7997 1937
Size: 5418 642 4594
Fragments arranged by size:
5418 4594 642
Acc65I G'GTAC_C
Cuts at: 7975 7975
Size: 10654
AclI AA'CG_TT
Cuts at: 1563 8781 9154 1563
Size: 7218 373 3063
Fragments arranged by size:
7218 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2472 2538 3099 3252 3498 3999 4272
Size: 508 332 66 561 153 246 501 273
Cuts at: 4272 5877 5913 6328 6684 7077 7667 7844 7854
Size: 1605 36 415 356 393 590 177 10
Cuts at: 7854 8717 10101 10369 1632
Size: 863 1384 268 1917
Fragments arranged by size:
1917 1605 1384 863 590 561 508 501
415 393 356 332 273 268 246 177
153 66 36 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10457
AflIII A'CryG_T
Cuts at: 1203 2336 2776 3216 9553 10530 1203
Size: 1133 440 440 6337 977 1327
Fragments arranged by size:
6337 1327 1133 977 440 440
AgeI A'CCGG_T
Cuts at: 7157 9485 7157
Size: 2328 8326
AhdI GACnn_n'nnGTC
Cuts at: 9383 9383
Size: 10654
AleI CACnn'nnGTG
Cuts at: 1166 3221 6015 6665 7864 1166
Size: 2055 2794 650 1199 3956
Fragments arranged by size:
3956 2794 2055 1199 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3599 1184
Size: 2415 8239
AlwNI CAG_nnn'CTG
Cuts at: 2312 2927 4022 4496 4511 4687 5534 7382 7645
Size: 615 1095 474 15 176 847 1848 263
Cuts at: 7645 7727 8397 9969 2312
Size: 82 670 1572 2997
Fragments arranged by size:
2997 1848 1572 1095 847 670 615 474
263 176 82 15
ApaLI G'TGCA_C
Cuts at: 8711 9867 8711
Size: 1156 9498
ApoI r'AATT_y
Cuts at: 1493 3109 3532 3710 6429 7322 7332 7605 7963
Size: 1616 423 178 2719 893 10 273 358
Cuts at: 7963 8189 8341 10450 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2719 2109 1697 1616 893 423 358 273
226 178 152 10
AseI AT'TA_AT
Cuts at: 160 1032 9208 160
Size: 872 8176 1606
Fragments arranged by size:
8176 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10654
AvaI C'yCGr_G
Cuts at: 1870 1943 6099 6360 7979 1870
Size: 73 4156 261 1619 4545
Fragments arranged by size:
4545 4156 1619 261 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6595 6628 6595
Size: 33 10621
BamHI G'GATC_C
Cuts at: 5191 7984 8351 5191
Size: 2793 367 7494
Fragments arranged by size:
7494 2793 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4818 7975 9430 618
Size: 325 217 627 3031 3157 1455 1842
Fragments arranged by size:
3157 3031 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4836 5921 6504 7136 7692 7973 1391
Size: 3445 1085 583 632 556 281 4072
Fragments arranged by size:
4072 3445 1085 632 583 556 281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2955 3355 4005 4136 4638 5135 5421
Size: 971 1056 400 650 131 502 497 286
Cuts at: 5421 7430 928
Size: 2009 4152
Fragments arranged by size:
4152 2009 1056 971 650 502 497 400
286 131
BbvCI CC'TCA_GC
Cuts at: 2373 5721 2373
Size: 3348 7306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8845 8879 1903
Size: 34 6908 34 3678
Fragments arranged by size:
6908 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3670 3746 8546 9756 3670
Size: 76 4800 1210 4568
Fragments arranged by size:
4800 4568 1210 76
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10654
BfrBI ATG'CAT
Cuts at: 3478 3478
Size: 10654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6226 6297 9265
Size: 107 122 71 631 605 4554 71 2968
Cuts at: 9265 136
Size: 1525
Fragments arranged by size:
4554 2968 1525 631 605 122 107 71
71
BglII A'GATC_T
Cuts at: 3118 4480 10649 3118
Size: 1362 6169 3123
Fragments arranged by size:
6169 3123 1362
BlpI GC'TnA_GC
Cuts at: 4661 8071 4661
Size: 3410 7244
Bme1580I G_kGCm'C
Cuts at: 1790 4821 6360 7753 8715 9871 1790
Size: 3031 1539 1393 962 1156 2573
Fragments arranged by size:
3031 2573 1539 1393 1156 962
BmgBI CAC'GTC
Cuts at: 1318 3219 4978 1318
Size: 1901 1759 6994
Fragments arranged by size:
6994 1901 1759
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2757 4384 5922
Size: 416 53 83 399 24 1340 1627 1538
Cuts at: 5922 7408 8385 9343 442
Size: 1486 977 958 1753
Fragments arranged by size:
1753 1627 1538 1486 1340 977 958 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10654
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4396 4088
Size: 308 10346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2265 3678 4282 4395 6195 6408 6465
Size: 932 94 1413 604 113 1800 213 57
Cuts at: 6465 6946 7535 7706 9314 1239
Size: 481 589 171 1608 2579
Fragments arranged by size:
2579 1800 1608 1413 932 604 589 481
213 171 113 94 57
Bpu10I CC'TnA_GC
Cuts at: 2373 5721 6025 2373
Size: 3348 304 7002
Fragments arranged by size:
7002 3348 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3649 5226 5367 5539 7613 8779 9644 9942 3649
Size: 1577 141 172 2074 1166 865 298 4361
Fragments arranged by size:
4361 2074 1577 1166 865 298 172 141
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4916 9317 882
Size: 617 3417 4401 2219
Fragments arranged by size:
4401 3417 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7951 8445 493
Size: 711 6747 494 2702
Fragments arranged by size:
6747 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 10648 10648
Size: 10654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6152 8461 8843 9478
Size: 53 83 186 936 4619 2309 382 635
Cuts at: 9478 275
Size: 1451
Fragments arranged by size:
4619 2309 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7157 9087 9485 9759 9906 10317 7157
Size: 1930 398 274 147 411 7494
Fragments arranged by size:
7494 1930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6674 6704 6923 6953 7113
Size: 30 165 30 5271 30 219 30 160
Cuts at: 7113 7143 7679 7709 1178
Size: 30 536 30 4123
Fragments arranged by size:
5271 4123 536 219 165 160 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 3255 4019 5267 5864 5911 5944 6117
Size: 1085 984 764 1248 597 47 33 173
Cuts at: 6117 6179 6466 7331 7610 1186
Size: 62 287 865 279 4230
Fragments arranged by size:
4230 1248 1085 984 865 764 597 287
279 173 62 47 33
BseYI C'CCAG_C
Cuts at: 3393 4687 5477 6092 6572 6943 7365 7580 7810
Size: 1294 790 615 480 371 422 215 230
Cuts at: 7810 9857 10353 3393
Size: 2047 496 3694
Fragments arranged by size:
3694 2047 1294 790 615 496 480 422
371 230 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2862 6720 7740 1163
Size: 573 1126 3858 1020 4077
Fragments arranged by size:
4077 3858 1126 1020 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8104 8865 9893 1540
Size: 275 186 6103 761 1028 2301
Fragments arranged by size:
6103 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3356 4382 5665 7692 8715 8800 9871 3356
Size: 1026 1283 2027 1023 85 1071 4139
Fragments arranged by size:
4139 2027 1283 1071 1026 1023 85
BsmI GAATG_Cn'
Cuts at: 4175 8165 8258 4175
Size: 3990 93 6571
Fragments arranged by size:
6571 3990 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2253 2253
Size: 10654
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3356 4382 4821 4836 5665 5921 6360
Size: 399 1566 1026 439 15 829 256 439
Cuts at: 6360 6504 7136 7692 7753 7973 8715 8800 9871
Size: 144 632 556 61 220 742 85 1071
Cuts at: 9871 1391
Size: 2174
Fragments arranged by size:
2174 1566 1071 1026 829 742 632 556
439 439 399 256 220 144 85 61
15
BspEI T'CCGG_A
Cuts at: 10317 10317
Size: 10654
BspHI T'CATG_A
Cuts at: 3409 7707 8543 10273 3409
Size: 4298 836 1730 3790
Fragments arranged by size:
4298 3790 1730 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2812 8009 844
Size: 1968 5197 3489
Fragments arranged by size:
5197 3489 1968
BsrBI CCG'CTC
Cuts at: 1394 1823 7275 8541 1394
Size: 429 5452 1266 3507
Fragments arranged by size:
5452 3507 1266 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5400 7838 9149 9323 65
Size: 1183 4152 2438 1311 174 1396
Fragments arranged by size:
4152 2438 1396 1311 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7157 9298 9485 1700
Size: 110 5347 2141 187 2869
Fragments arranged by size:
5347 2869 2141 187 110
BsrGI T'GTAC_A
Cuts at: 96 2773 96
Size: 2677 7977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10654
BssSI C'ACGA_G
Cuts at: 5790 6222 6558 7008 8714 9726 5790
Size: 432 336 450 1706 1012 6718
Fragments arranged by size:
6718 1706 1012 450 432 336
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2846 3061 6083 794
Size: 2052 215 3022 5365
Fragments arranged by size:
5365 3022 2052 215
BstBI TT'CG_AA
Cuts at: 2007 9458 2007
Size: 7451 3203
BstEII G'GTnAC_C
Cuts at: 1184 5273 5927 1184
Size: 4089 654 5911
Fragments arranged by size:
5911 4089 654
BstXI CCAn_nnnn'nTGG
Cuts at: 5590 5603 6655 5590
Size: 13 1052 9589
Fragments arranged by size:
9589 1052 13
BstYI r'GATC_y
Cuts at: 1972 3118 3544 4480 5090 5191 7701 7984 8351
Size: 1146 426 936 610 101 2510 283 367
Cuts at: 8351 8736 8753 10205 10291 10303 10649 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2510 1977 1452 1146 936 610 426 385
367 346 283 101 86 17 12
BstZ17I GTA'TAC
Cuts at: 7356 7356
Size: 10654
Bsu36I CC'TnA_GG
Cuts at: 2928 3540 5923 2928
Size: 612 2383 7659
Fragments arranged by size:
7659 2383 612
BtgI C'CryG_G
Cuts at: 513 1065 5505 7017 7242 513
Size: 552 4440 1512 225 3925
Fragments arranged by size:
4440 3925 1512 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9915
Fragments arranged by size:
9915 683 56
BtsI GCAGTG_nn'
Cuts at: 2398 6022 7312 8170 8964 8984 2398
Size: 3624 1290 858 794 20 4068
Fragments arranged by size:
4068 3624 1290 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6755 6790 529
Size: 35 6191 35 4393
Fragments arranged by size:
6191 4393 35 35
DraI TTT'AAA
Cuts at: 7958 7958
Size: 10654
DraIII CAC_nnn'GTG
Cuts at: 4052 4052
Size: 10654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9661 809
Size: 507 8345 1802
Fragments arranged by size:
8345 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5734 7245 7266
Size: 54 1425 50 275 18 3904 1511 21
Cuts at: 7266 8101 8990 10619 8
Size: 835 889 1629 43
Fragments arranged by size:
3904 1629 1511 1425 889 835 275 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 8101 1537
Size: 275 6289 4090
Fragments arranged by size:
6289 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2430 2442 5031 5244 5700 5853 7053 7173
Size: 1077 12 2589 213 456 153 1200 120
Cuts at: 7173 7308 8584 1353
Size: 135 1276 3423
Fragments arranged by size:
3423 2589 1276 1200 1077 456 213 153
135 120 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9241 9615 9761 387
Size: 1064 138 7652 374 146 1280
Fragments arranged by size:
7652 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7690 7690
Size: 10654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2171 2265 2472 2538 3099 3252
Size: 393 508 31 94 207 66 561 153
Cuts at: 3252 3498 3678 3999 4272 4282 4395 5877 5913
Size: 246 180 321 273 10 113 1482 36
Cuts at: 5913 6195 6328 6408 6465 6684 6946 7077 7535
Size: 282 133 80 57 219 262 131 458
Cuts at: 7535 7667 7706 7844 7854 8717 9314 10101 10369
Size: 132 39 138 10 863 597 787 268
Cuts at: 10369 1239
Size: 1524
Fragments arranged by size:
1524 1482 863 787 597 561 508 458
393 321 282 273 268 262 246 219
207 180 153 138 133 132 131 113
94 80 66 57 39 36 31 10
10
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 4767 6129 6680 3089
Size: 1678 1362 551 7063
Fragments arranged by size:
7063 1678 1362 551
EcoO109I rG'GnC_Cy
Cuts at: 3081 5329 5524 7190 3081
Size: 2248 195 1666 6545
Fragments arranged by size:
6545 2248 1666 195
EcoRI G'AATT_C
Cuts at: 3109 3532 7605 7963 8341 3109
Size: 423 4073 358 378 5422
Fragments arranged by size:
5422 4073 423 378 358
EcoRV GAT'ATC
Cuts at: 2713 4777 5941 6886 2713
Size: 2064 1164 945 6481
Fragments arranged by size:
6481 2064 1164 945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7672 7672
Size: 10654
FspI TGC'GCA
Cuts at: 9160 9160
Size: 10654
HaeII r_GCGC'y
Cuts at: 1678 9801 1678
Size: 8123 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2513 2545 2921
Size: 32 135 32 635 32 274 32 376
Cuts at: 2921 2953 2978 3010 3995 4027 4108 4140 4376
Size: 32 25 32 985 32 81 32 236
Cuts at: 4376 4408 4472 4504 4889 4915 4921 4947 5666
Size: 32 64 32 385 26 6 26 719
Cuts at: 5666 5698 5930 5962 6323 6355 6461 6493 7052
Size: 32 232 32 361 32 106 32 559
Cuts at: 7052 7084 7679 7711 9295 9327 9369 9401 1373
Size: 32 595 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1584 985 719 635 595 559 385
376 361 274 236 232 135 106 81
64 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 26 26 25 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 5297 7998 8244 669
Size: 988 281 1002 2357 2701 246 3079
Fragments arranged by size:
3079 2701 2357 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4269 4717 748
Size: 3521 448 6685
Fragments arranged by size:
6685 3521 448
HpaI GTT'AAC
Cuts at: 8244 8244
Size: 10654
KpnI G_GTAC'C
Cuts at: 7979 7979
Size: 10654
MfeI C'AATT_G
Cuts at: 5865 8253 5865
Size: 2388 8266
MluI A'CGCG_T
Cuts at: 10530 10530
Size: 10654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 2193 2433 2727 6547 9768 9952 1305
Size: 735 153 240 294 3820 3221 184 2007
Fragments arranged by size:
3820 3221 2007 735 294 240 184 153
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5736 7247 7268 10
Size: 54 1425 343 3904 1511 21 3396
Fragments arranged by size:
3904 3396 1511 1425 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3221 5138 5736 6015 6665 7746
Size: 648 1581 474 1917 598 279 650 1081
Cuts at: 7746 7864 7935 8612 8971 9130 518
Size: 118 71 677 359 159 2042
Fragments arranged by size:
2042 1917 1581 1081 677 650 648 598
474 359 279 159 118 71
MspA1I CmG'CkG
Cuts at: 1606 2243 6063 6281 6655 7580 7846 8070 8747
Size: 637 3820 218 374 925 266 224 677
Cuts at: 8747 9895 10140 1606
Size: 1148 245 2120
Fragments arranged by size:
3820 2120 1148 925 677 637 374 266
245 224 218
NcoI C'CATG_G
Cuts at: 513 1065 5505 7017 7242 513
Size: 552 4440 1512 225 3925
Fragments arranged by size:
4440 3925 1512 552 225
NdeI CA'TA_TG
Cuts at: 387 2599 387
Size: 2212 8442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10654
NotI GC'GGCC_GC
Cuts at: 8101 8101
Size: 10654
NsiI A_TGCA'T
Cuts at: 3480 3480
Size: 10654
NspI r_CATG'y
Cuts at: 1132 1229 2340 2780 2824 8012 9557 1132
Size: 97 1111 440 44 5188 1545 2229
Fragments arranged by size:
5188 2229 1545 1111 440 97 44
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10654
PciI A'CATG_T
Cuts at: 2336 2776 9553 2336
Size: 440 6777 3437
Fragments arranged by size:
6777 3437 440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4249 4687 4793 5356 5506 1108
Size: 3141 438 106 563 150 6256
Fragments arranged by size:
6256 3141 563 438 150 106
PfoI T'CCnGG_A
Cuts at: 5766 6481 7683 5766
Size: 715 1202 8737
Fragments arranged by size:
8737 1202 715
PmeI GTTT'AAAC
Cuts at: 7958 7958
Size: 10654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3616 3648 8723 8755 10236 10268 3616
Size: 32 5075 32 1481 32 4002
Fragments arranged by size:
5075 4002 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3081 5329 5524 3081
Size: 2248 195 8211
Fragments arranged by size:
8211 2248 195
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10654
PsiI TTA'TAA
Cuts at: 8224 8224
Size: 10654
PstI C_TGCA'G
Cuts at: 830 2907 3066 4501 8006 830
Size: 2077 159 1435 3505 3478
Fragments arranged by size:
3505 3478 2077 1435 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10654
PvuII CAG'CTG
Cuts at: 2243 6281 7580 7846 2243
Size: 4038 1299 266 5051
Fragments arranged by size:
5051 4038 1299 266
SacI G_AGCT'C
Cuts at: 7692 7692
Size: 10654
SalI G'TCGA_C
Cuts at: 1936 7996 1936
Size: 6060 4594
SanDI GG'GwC_CC
Cuts at: 5524 5524
Size: 10654
SapI GCTCTTCn'nnn_
Cuts at: 5853 7173 5853
Size: 1320 9334
SbfI CC_TGCA'GG
Cuts at: 8006 8006
Size: 10654
ScaI AGT'ACT
Cuts at: 4849 5437 7475 8902 10614 4849
Size: 588 2038 1427 1712 4889
Fragments arranged by size:
4889 2038 1712 1427 588
SfcI C'TryA_G
Cuts at: 826 1045 2903 3062 4497 4512 6745 7110 7296
Size: 219 1858 159 1435 15 2233 365 186
Cuts at: 7296 8002 9137 9818 10009 826
Size: 706 1135 681 191 1471
Fragments arranged by size:
2233 1858 1471 1435 1135 706 681 365
219 191 186 159 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10654
SgrAI Cr'CCGG_yG
Cuts at: 1700 7157 1700
Size: 5457 5197
SmaI CCC'GGG
Cuts at: 1872 7981 1872
Size: 6109 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3628 5241 5382 5518 7592 8758
Size: 197 926 1685 1613 141 136 2074 1166
Cuts at: 8758 9659 9921 820
Size: 901 262 1553
Fragments arranged by size:
2074 1685 1613 1553 1166 926 901 262
197 141 136
SnaBI TAC'GTA
Cuts at: 493 7951 8445 493
Size: 7458 494 2702
Fragments arranged by size:
7458 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10654
SphI G_CATG'C
Cuts at: 1132 8012 1132
Size: 6880 3774
SspI AAT'ATT
Cuts at: 5 52 8578 5
Size: 47 8526 2081
Fragments arranged by size:
8526 2081 47
StuI AGG'CCT
Cuts at: 1517 5462 6183 1517
Size: 3945 721 5988
Fragments arranged by size:
5988 3945 721
StyI C'CwwG_G
Cuts at: 513 1065 2509 4132 4449 5505 5958 6184 6294
Size: 552 1444 1623 317 1056 453 226 110
Cuts at: 6294 6804 7017 7242 8093 513
Size: 510 213 225 851 3074
Fragments arranged by size:
3074 1623 1444 1056 851 552 510 453
317 226 225 213 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3009 5221 6383 7858 8681 8698 8851
Size: 1255 1429 2212 1162 1475 823 17 153
Cuts at: 8851 9036 9481 325
Size: 185 445 1498
Fragments arranged by size:
2212 1498 1475 1429 1255 1162 823 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2773 3766 4847
Size: 274 80 33 51 1443 796 993 1081
Cuts at: 4847 5435 5585 6741 7473 8900 10612 96
Size: 588 150 1156 732 1427 1712 138
Fragments arranged by size:
1712 1443 1427 1156 1081 993 796 732
588 274 150 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2394 2409 2426 3005 3037 3348 3369 3380
Size: 17 15 17 579 32 311 21 11
Cuts at: 3380 3401 6988 7020 7113 7145 7489 7521 10614
Size: 21 3587 32 93 32 344 32 3093
Cuts at: 10614 10646 2377
Size: 32 2385
Fragments arranged by size:
3587 3093 2385 579 344 311 93 32
32 32 32 32 21 21 17 17
15 11
Tth111I GACn'n_nGTC
Cuts at: 1316 5134 1316
Size: 3818 6836
XbaI T'CTAG_A
Cuts at: 2131 7990 2131
Size: 5859 4795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6446 7261 6446
Size: 815 9839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10654
XmaI C'CCGG_G
Cuts at: 1870 7979 1870
Size: 6109 4545
XmnI GAAnn'nnTTC
Cuts at: 7475 8783 7475
Size: 1308 9346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8462 276
Size: 53 83 186 936 6928 2468
Fragments arranged by size:
6928 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI