Restriction map for
pFN21ASDB0037
(Circular) (Six-base) MAPSORT of: pFN21ASDB0037.seq Check: 748 from: 1 to: 6142
pFN21ASDB0037 6142 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2676 3952 278
Size: 53 83 186 936 1140 1276 2468
Fragments arranged by size:
2468 1276 1140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3312 3485 1937
Size: 1375 173 4594
Fragments arranged by size:
4594 1375 173
Acc65I G'GTAC_C
Cuts at: 3169 3463 3169
Size: 294 5848
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2798 4205 5589 5857 1632
Size: 995 171 1407 1384 268 1917
Fragments arranged by size:
1917 1407 1384 995 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 2991 1166
Size: 1825 4317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 3885 5457 3885
Size: 1572 4570
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2552 2572 3399 3451 3677 3829 5938 1493
Size: 1059 20 827 52 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 827 226 152 52 20
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2757 3467 1870
Size: 73 814 710 4545
Fragments arranged by size:
4545 814 710 73
BamHI G'GATC_C
Cuts at: 3261 3472 3839 3261
Size: 211 367 5564
Fragments arranged by size:
5564 367 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 3169 3463 4918 618
Size: 325 217 627 351 1031 294 1455 1842
Fragments arranged by size:
1842 1455 1031 627 351 325 294 217
BanII G_rGCy'C
Cuts at: 1391 3245 3461 1391
Size: 1854 216 4072
Fragments arranged by size:
4072 1854 216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2668 3153 928
Size: 971 769 485 3917
Fragments arranged by size:
3917 971 769 485
BbvCI CC'TCA_GC
Cuts at: 3025 3025
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2832 6137 2832
Size: 3305 2837
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2859 4203 5359 1790
Size: 1069 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 1069
BmgBI CAC'GTC
Cuts at: 1318 2683 3062 1318
Size: 1365 379 4398
Fragments arranged by size:
4398 1365 379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4802 1239
Size: 3563 2579
Bpu10I CC'TnA_GC
Cuts at: 3025 3025
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 2663 4805 882
Size: 617 930 234 2142 2219
Fragments arranged by size:
2219 2142 930 617 234
BsaAI yAC'GTr
Cuts at: 493 1204 3439 3933 493
Size: 711 2235 494 2702
Fragments arranged by size:
2702 2235 711 494
BsaBI GATnn'nnATC
Cuts at: 2039 2292 3036 6136 2039
Size: 253 744 3100 2045
Fragments arranged by size:
3100 2045 744 253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 3949 4331 4966
Size: 53 83 186 936 1140 1276 382 635
Cuts at: 4966 275
Size: 1451
Fragments arranged by size:
1451 1276 1140 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5917
Fragments arranged by size:
5917 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2978 1186
Size: 1792 4350
BseYI C'CCAG_C
Cuts at: 2225 5345 5841 2225
Size: 3120 496 2526
Fragments arranged by size:
3120 2526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4203 4288 5359 4203
Size: 85 1071 4986
Fragments arranged by size:
4986 1071 85
BsmI GAATG_Cn'
Cuts at: 2520 3653 3746 2520
Size: 1133 93 4916
Fragments arranged by size:
4916 1133 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2859 3245 3461 4203 4288 5359 1391
Size: 399 1069 386 216 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 399 386 216 85
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2102 2769 4637 4811 65
Size: 1183 854 667 1868 174 1396
Fragments arranged by size:
1868 1396 1183 854 667 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 4786 4973 1700
Size: 110 265 2711 187 2869
Fragments arranged by size:
2869 2711 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 2007 4946 2007
Size: 2939 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2832 3261 3295 3472 3839 4224 4241 5693
Size: 860 429 34 177 367 385 17 1452
Cuts at: 5693 5779 5791 6137 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 860 429 385 367 346 177
86 34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4633
Fragments arranged by size:
4633 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3023 487
Size: 683 56 1797 3606
Fragments arranged by size:
3606 1797 683 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 2394 3446 2394
Size: 1052 5090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2073 3589
Size: 54 1425 50 275 18 195 48 1516
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1516 1425 889 275 195 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 2928 4072 1353
Size: 993 582 1144 3423
Fragments arranged by size:
3423 1144 993 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2718 4729 5103 5249 387
Size: 1064 138 1129 2011 374 146 1280
Fragments arranged by size:
2011 1280 1129 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 2798 4205 4802 5589 5857 1239
Size: 393 995 171 1407 597 787 268 1524
Fragments arranged by size:
1524 1407 995 787 597 393 268 171
EcoNI CCTnn'n_nnAGG
Cuts at: 2175 2175
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3035 3035
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 3330 3353 3362
Size: 32 135 32 1339 32 387 23 9
Cuts at: 3362 3385 4783 4815 4857 4889 1373
Size: 23 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 1339 387 135 42 32 32
32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3173 3467 3173
Size: 294 5848
MfeI C'AATT_G
Cuts at: 2580 3741 2580
Size: 1161 4981
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 5256 5440 1305
Size: 1032 2919 184 2007
Fragments arranged by size:
2919 2007 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 10
Size: 54 1425 343 195 4125
Fragments arranged by size:
4125 1425 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2991 4100 4459 4618 518
Size: 648 1278 547 1109 359 159 2042
Fragments arranged by size:
2042 1278 1109 648 547 359 159
MspA1I CmG'CkG
Cuts at: 1606 3558 4235 5383 5628 1606
Size: 1952 677 1148 245 2120
Fragments arranged by size:
2120 1952 1148 677 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4633
Fragments arranged by size:
4633 957 552
NdeI CA'TA_TG
Cuts at: 387 2845 387
Size: 2458 3684
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3500 5045 1132
Size: 97 2271 1545 2229
Fragments arranged by size:
2271 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 1108
Size: 1578 4564
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 2819 4390 6102 2819
Size: 1571 1712 2859
Fragments arranged by size:
2859 1712 1571
SfcI C'TryA_G
Cuts at: 826 1045 3309 3490 4625 5306 5497 826
Size: 219 2264 181 1135 681 191 1471
Fragments arranged by size:
2264 1471 1135 681 219 191 181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4246 5147 5409 820
Size: 197 926 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3439 3933 493
Size: 2946 494 2702
Fragments arranged by size:
2946 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2022 3581 513
Size: 552 957 1559 3074
Fragments arranged by size:
3074 1559 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2817 4388 6100
Size: 274 80 33 51 1443 840 1571 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1712 1571 1443 840 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3238 3270 6102 6134 3238
Size: 32 2832 32 3246
Fragments arranged by size:
3246 2832 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 2713 3478 2713
Size: 765 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2674 3950 276
Size: 53 83 186 936 1140 1276 2468
Fragments arranged by size:
2468 1276 1140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI