Restriction map for
pFN21ASDB0036
(Circular) (Six-base) MAPSORT of: pFN21ASDB0036.seq Check: 3438 from: 1 to: 6664
pFN21ASDB0036 6664 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2384 4007 1937
Size: 447 1623 4594
Fragments arranged by size:
4594 1623 447
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3342 3429 4727 6111 6379 1632
Size: 1710 87 1298 1384 268 1917
Fragments arranged by size:
1917 1710 1384 1298 268 87
AflII C'TTAA_G
Cuts at: 820 1017 2338 820
Size: 197 1321 5146
Fragments arranged by size:
5146 1321 197
AflIII A'CryG_T
Cuts at: 1203 2325 2379 5563 6540 1203
Size: 1122 54 3184 977 1327
Fragments arranged by size:
3184 1327 1122 977 54
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 2462 1166
Size: 1296 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6664
AlwNI CAG_nnn'CTG
Cuts at: 2038 4407 5979 2038
Size: 2369 1572 2723
Fragments arranged by size:
2723 2369 1572
ApaI G_GGCC'C
Cuts at: 2133 2133
Size: 6664
ApaLI G'TGCA_C
Cuts at: 2164 4721 5877 2164
Size: 2557 1156 2951
Fragments arranged by size:
2951 2557 1156
ApoI r'AATT_y
Cuts at: 1493 3837 3973 4199 4351 6460 1493
Size: 2344 136 226 152 2109 1697
Fragments arranged by size:
2344 2109 1697 226 152 136
AseI AT'TA_AT
Cuts at: 160 1032 2670 5218 160
Size: 872 1638 2548 1606
Fragments arranged by size:
2548 1638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 3989 1870
Size: 73 2046 4545
Fragments arranged by size:
4545 2046 73
BamHI G'GATC_C
Cuts at: 3994 4361 3994
Size: 367 6297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3781 3985 5440 618
Size: 325 217 627 1994 204 1455 1842
Fragments arranged by size:
1994 1842 1455 627 325 217 204
BanII G_rGCy'C
Cuts at: 1391 2133 3983 1391
Size: 742 1850 4072
Fragments arranged by size:
4072 1850 742
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 2834 2934 928
Size: 971 340 595 100 4658
Fragments arranged by size:
4658 971 595 340 100
BbvCI CC'TCA_GC
Cuts at: 3112 3112
Size: 6664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4855 4889 1903
Size: 34 2918 34 3678
Fragments arranged by size:
3678 2918 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2098 3115 4556 5766 2098
Size: 1017 1441 1210 2996
Fragments arranged by size:
2996 1441 1210 1017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6659 6659
Size: 6664
BlpI GC'TnA_GC
Cuts at: 4081 4081
Size: 6664
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2168 4725 5881 1790
Size: 343 35 2557 1156 2573
Fragments arranged by size:
2573 2557 1156 343 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3729 4395 5353
Size: 416 53 83 399 24 2312 666 958
Cuts at: 5353 442
Size: 1753
Fragments arranged by size:
2312 1753 958 666 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3408 5324 1239
Size: 2169 1916 2579
Fragments arranged by size:
2579 2169 1916
Bpu10I CC'TnA_GC
Cuts at: 3112 3112
Size: 6664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4789 5654 5952 4789
Size: 865 298 5501
Fragments arranged by size:
5501 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3901 5327 882
Size: 617 2402 1426 2219
Fragments arranged by size:
2402 2219 1426 617
BsaAI yAC'GTr
Cuts at: 493 1204 3961 4455 493
Size: 711 2757 494 2702
Fragments arranged by size:
2757 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5097 5495 5769 5916 6327 5097
Size: 398 274 147 411 5434
Fragments arranged by size:
5434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3270 3300 3406 3436 1178
Size: 30 165 30 1867 30 106 30 4406
Fragments arranged by size:
4406 1867 165 106 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6664
BseYI C'CCAG_C
Cuts at: 2061 5867 6363 2061
Size: 3806 496 2362
Fragments arranged by size:
3806 2362 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 1163
Size: 573 1381 4710
Fragments arranged by size:
4710 1381 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 3943 4725 4810 5881 2168
Size: 1775 782 85 1071 2951
Fragments arranged by size:
2951 1775 1071 782 85
BsmI GAATG_Cn'
Cuts at: 3772 4175 4268 3772
Size: 403 93 6168
Fragments arranged by size:
6168 403 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2168 3943 3983 4725 4810 5881
Size: 399 343 35 1775 40 742 85 1071
Cuts at: 5881 1391
Size: 2174
Fragments arranged by size:
2174 1775 1071 742 399 343 85 40
35
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 3394 3903 4553 6283 3394
Size: 509 650 1730 3775
Fragments arranged by size:
3775 1730 650 509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4019 844
Size: 3175 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4551 1394
Size: 429 2728 3507
Fragments arranged by size:
3507 2728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2584 5159 5333 65
Size: 1183 1336 2575 174 1396
Fragments arranged by size:
2575 1396 1336 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 5308 5495 1700
Size: 110 265 3233 187 2869
Fragments arranged by size:
3233 2869 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 3235 96
Size: 3139 3525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 2117 4724 5736 2117
Size: 2607 1012 3045
Fragments arranged by size:
3045 2607 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6664
BstBI TT'CG_AA
Cuts at: 2007 5468 2007
Size: 3461 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 3643 3643
Size: 6664
BstYI r'GATC_y
Cuts at: 1972 3725 3994 4361 4746 4763 6215 6301 6313
Size: 1753 269 367 385 17 1452 86 12
Cuts at: 6313 6659 1972
Size: 346 1977
Fragments arranged by size:
1977 1753 1452 385 367 346 269 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5925
Fragments arranged by size:
5925 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2943 4180 4974 4994 2052
Size: 891 1237 794 20 3722
Fragments arranged by size:
3722 1237 891 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6629
DraI TTT'AAA
Cuts at: 2415 3036 3968 2415
Size: 621 932 5111
Fragments arranged by size:
5111 932 621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 4111 5000
Size: 54 1425 50 275 18 199 2082 889
Cuts at: 5000 6629 8
Size: 1629 43
Fragments arranged by size:
2082 1629 1425 889 275 199 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2462 4594 1353
Size: 1109 2132 3423
Fragments arranged by size:
3423 2132 1109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5251 5625 5771 387
Size: 1064 138 3662 374 146 1280
Fragments arranged by size:
3662 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3342 3408 3429 4727 5324 6111 6379
Size: 393 1710 66 21 1298 597 787 268
Cuts at: 6379 1239
Size: 1524
Fragments arranged by size:
1710 1524 1298 787 597 393 268 66
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 3933 2825
Size: 1108 5556
EcoO109I rG'GnC_Cy
Cuts at: 2129 2830 2129
Size: 701 5963
EcoRI G'AATT_C
Cuts at: 3837 3973 4351 3837
Size: 136 378 6150
Fragments arranged by size:
6150 378 136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6664
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 3049 5811 1678
Size: 1371 2762 2531
Fragments arranged by size:
2762 2531 1371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3376 3408 3455 3487 5305
Size: 32 135 32 1804 32 47 32 1818
Cuts at: 5305 5337 5379 5411 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1818 1804 135 47 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3463 3698 3717 4008 4254
Size: 988 281 1311 214 235 19 291 246
Cuts at: 4254 669
Size: 3079
Fragments arranged by size:
3079 1311 988 291 281 246 235 214
19
HindIII A'AGCT_T
Cuts at: 748 3030 3304 748
Size: 2282 274 4108
Fragments arranged by size:
4108 2282 274
HpaI GTT'AAC
Cuts at: 3717 4254 3717
Size: 537 6127
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2658 2773 3388 5778 5962 1305
Size: 1353 115 615 2390 184 2007
Fragments arranged by size:
2390 2007 1353 615 184 115
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4842
Fragments arranged by size:
4842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 4622 4981 5140 518
Size: 648 1296 2160 359 159 2042
Fragments arranged by size:
2160 2042 1296 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2906 3449 4080 4757 5905 6150 1606
Size: 1300 543 631 677 1148 245 2120
Fragments arranged by size:
2120 1300 1148 677 631 543 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6112
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 2383 4022 5567 1132
Size: 97 1154 1639 1545 2229
Fragments arranged by size:
2229 1639 1545 1154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6664
PciI A'CATG_T
Cuts at: 2379 5563 2379
Size: 3184 3480
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6664
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2129 2129
Size: 6664
PstI C_TGCA'G
Cuts at: 830 3094 4016 830
Size: 2264 922 3478
Fragments arranged by size:
3478 2264 922
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2906 3449 2906
Size: 543 6121
RsrII CG'GwC_CG
Cuts at: 2044 2044
Size: 6664
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 4912 6624 4912
Size: 1712 4952
SexAI A'CCwGG_T
Cuts at: 3700 3700
Size: 6664
SfcI C'TryA_G
Cuts at: 826 1045 2901 3090 4012 5147 5828 6019 826
Size: 219 1856 189 922 1135 681 191 1471
Fragments arranged by size:
1856 1471 1135 922 681 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 4768 5669 5931 820
Size: 197 926 395 2430 901 262 1553
Fragments arranged by size:
2430 1553 926 901 395 262 197
SnaBI TAC'GTA
Cuts at: 493 3961 4455 493
Size: 3468 494 2702
Fragments arranged by size:
3468 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 2687 4588 5
Size: 47 2635 1901 2081
Fragments arranged by size:
2635 2081 1901 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6664
StyI C'CwwG_G
Cuts at: 513 1065 2874 4103 513
Size: 552 1809 1229 3074
Fragments arranged by size:
3074 1809 1229 552
SwaI ATTT'AAAT
Cuts at: 2415 2415
Size: 6664
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 2227 3243 4691 4708 4861 5046
Size: 1255 481 166 1016 1448 17 153 185
Cuts at: 5046 5491 325
Size: 445 1498
Fragments arranged by size:
1498 1448 1255 1016 481 445 185 166
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3235 3269 4910
Size: 274 80 33 51 1443 1258 34 1641
Cuts at: 4910 6622 96
Size: 1712 138
Fragments arranged by size:
1712 1641 1443 1258 274 138 80 51
34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3406 3438 6624 6656 3406
Size: 32 3186 32 3414
Fragments arranged by size:
3414 3186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3759 3759
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 4793 4793
Size: 6664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII
SanDI SapI SfiI SfoI SrfI