Restriction map for
pFN21ASDB0035
(Circular) (Six-base) MAPSORT of: pFN21ASDB0035.seq Check: 1835 from: 1 to: 6136
pFN21ASDB0035 6136 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 2259 2662 3055 3457 2259
Size: 403 393 402 4938
Fragments arranged by size:
4938 403 402 393
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3186 4199 5583 5851 1632
Size: 1554 1013 1384 268 1917
Fragments arranged by size:
1917 1554 1384 1013 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 2269 1166
Size: 1103 5033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 2449
Size: 32 6104
AlwNI CAG_nnn'CTG
Cuts at: 2828 3040 3086 3110 3879 5451 2828
Size: 212 46 24 769 1572 3513
Fragments arranged by size:
3513 1572 769 212 46 24
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 2113 2296 2439 3273 3302 3329 3445 3671
Size: 620 183 143 834 29 27 116 226
Cuts at: 3671 3823 5932 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 834 620 226 183 152 143
116 29 27
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2736 3461 1870
Size: 73 793 725 4545
Fragments arranged by size:
4545 793 725 73
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2662 2780 3055 3170
Size: 325 217 627 472 403 118 275 115
Cuts at: 3170 3457 4912 618
Size: 287 1455 1842
Fragments arranged by size:
1842 1455 627 472 403 325 287 275
217 118 115
BanII G_rGCy'C
Cuts at: 1391 2646 3455 1391
Size: 1255 809 4072
Fragments arranged by size:
4072 1255 809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2556 2749 928
Size: 971 317 340 193 4315
Fragments arranged by size:
4315 971 340 317 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2783 3175 4197 5353 1790
Size: 993 392 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 993 392
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2793 3867 4825
Size: 416 53 83 399 24 1376 1074 958
Cuts at: 4825 442
Size: 1753
Fragments arranged by size:
1753 1376 1074 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2850 3082 4796 1239
Size: 1611 232 1714 2579
Fragments arranged by size:
2579 1714 1611 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 4261 5126 5424 2921
Size: 1340 865 298 3633
Fragments arranged by size:
3633 1340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4799 882
Size: 617 3300 2219
Fragments arranged by size:
3300 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3433 3927 493
Size: 711 2229 494 2702
Fragments arranged by size:
2702 2229 711 494
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4325 4960 275
Size: 53 83 186 936 2410 382 635 1451
Fragments arranged by size:
2410 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3154 4569 4967 5241 5388 5799 3154
Size: 1415 398 274 147 411 3491
Fragments arranged by size:
3491 1415 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2912 2942 1178
Size: 30 165 30 1509 30 4372
Fragments arranged by size:
4372 1509 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2715 2736 1186
Size: 1529 21 4586
Fragments arranged by size:
4586 1529 21
BseYI C'CCAG_C
Cuts at: 5339 5835 5339
Size: 496 5640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5563
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 4197 4282 5353 2646
Size: 1551 85 1071 3429
Fragments arranged by size:
3429 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 2053 3647 3740 2053
Size: 1594 93 4449
Fragments arranged by size:
4449 1594 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2646 2783 3175 3455 4197 4282 5353
Size: 399 856 137 392 280 742 85 1071
Cuts at: 5353 1391
Size: 2174
Fragments arranged by size:
2174 1071 856 742 399 392 280 137
85
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3223 4631 4805 65
Size: 1183 1975 1408 174 1396
Fragments arranged by size:
1975 1408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 2007 4940 2007
Size: 2933 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2271 2451 2271
Size: 180 5956
BstYI r'GATC_y
Cuts at: 1972 2457 2624 2886 3466 3833 4218 4235 5687
Size: 485 167 262 580 367 385 17 1452
Cuts at: 5687 5773 5785 6131 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 580 485 385 367 346 262
167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3051 3365 3051
Size: 314 5822
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3652 4446 4466 3196
Size: 456 794 20 4866
Fragments arranged by size:
4866 794 456 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DraIII CAC_nnn'GTG
Cuts at: 2207 2207
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3005 5143 809
Size: 507 1689 2138 1802
Fragments arranged by size:
2138 1802 1689 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3264 3583 4472
Size: 54 1425 50 275 18 1434 319 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1434 1425 889 319 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4066 1353
Size: 2713 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2899 4723 5097 5243 387
Size: 1064 138 1310 1824 374 146 1280
Fragments arranged by size:
1824 1310 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2644 2644
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2850 3082 3186 4199 4796 5583 5851
Size: 393 1218 232 104 1013 597 787 268
Cuts at: 5851 1239
Size: 1524
Fragments arranged by size:
1524 1218 1013 787 597 393 268 232
104
EcoRI G'AATT_C
Cuts at: 2296 2439 3445 3823 2296
Size: 143 1006 378 4609
Fragments arranged by size:
4609 1006 378 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2397 3191 2397
Size: 794 5342
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2537 5283 1678
Size: 859 2746 2531
Fragments arranged by size:
2746 2531 859
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3104 3136 4777 4809 4851
Size: 32 135 32 1532 32 1641 32 42
Cuts at: 4851 4883 1373
Size: 32 2626
Fragments arranged by size:
2626 1641 1532 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2404 3480 3726 669
Size: 988 281 466 1076 246 3079
Fragments arranged by size:
3079 1076 988 466 281 246
HindIII A'AGCT_T
Cuts at: 748 3376 748
Size: 2628 3508
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 2263 2666 3059 3461 2263
Size: 403 393 402 4938
Fragments arranged by size:
4938 403 402 393
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2332 2955 5250 5434 1305
Size: 1027 623 2295 184 2007
Fragments arranged by size:
2295 2007 1027 623 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4314
Fragments arranged by size:
4314 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2269 2474 4094 4453 4612 518
Size: 648 1103 205 1620 359 159 2042
Fragments arranged by size:
2042 1620 1103 648 359 205 159
MspA1I CmG'CkG
Cuts at: 1606 2621 2791 3552 4229 5377 5622 1606
Size: 1015 170 761 677 1148 245 2120
Fragments arranged by size:
2120 1148 1015 761 677 245 170
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5584
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 2487 3095 3494 5039 1132
Size: 97 1258 608 399 1545 2229
Fragments arranged by size:
2229 1545 1258 608 399 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PfoI T'CCnGG_A
Cuts at: 3009 3009
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2220 3252 1613
Size: 607 1032 4497
Fragments arranged by size:
4497 1032 607
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PstI C_TGCA'G
Cuts at: 830 3247 3488 830
Size: 2417 241 3478
Fragments arranged by size:
3478 2417 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
SacI G_AGCT'C
Cuts at: 2646 2646
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 3243 3484 4619 5300 5491 826
Size: 219 2198 241 1135 681 191 1471
Fragments arranged by size:
2198 1471 1135 681 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 2738 3463 1872
Size: 866 725 4545
Fragments arranged by size:
4545 866 725
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 4240 5141 5403 820
Size: 197 926 957 1340 901 262 1553
Fragments arranged by size:
1553 1340 957 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3433 3927 493
Size: 2940 494 2702
Fragments arranged by size:
2940 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 3422 1517
Size: 1905 4231
StyI C'CwwG_G
Cuts at: 513 1065 3575 513
Size: 552 2510 3074
Fragments arranged by size:
3074 2510 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4163 4180 4333 4518 4963 325
Size: 1255 2583 17 153 185 445 1498
Fragments arranged by size:
2583 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 2857 3472 2857
Size: 615 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 2736 3461 1870
Size: 866 725 4545
Fragments arranged by size:
4545 866 725
XmnI GAAnn'nnTTC
Cuts at: 2691 4265 2691
Size: 1574 4562
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI