Restriction map for
pFN21ASDB0034
(Circular) (Six-base) MAPSORT of: pFN21ASDB0034.seq Check: 9239 from: 1 to: 6838
pFN21ASDB0034 6838 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2770 3639 3745 3832 4048 2770
Size: 869 106 87 216 5560
Fragments arranged by size:
5560 869 216 106 87
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 4181 1937
Size: 1293 951 4594
Fragments arranged by size:
4594 1293 951
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2143 2660 2684 3113 3344 4901 6285 6553
Size: 511 517 24 429 231 1557 1384 268
Cuts at: 6553 1632
Size: 1917
Fragments arranged by size:
1917 1557 1384 517 511 429 268 231
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 3498 5737 6714 1203
Size: 2295 2239 977 1327
Fragments arranged by size:
2295 2239 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 2273 2972 3173 1166
Size: 1107 699 201 4831
Fragments arranged by size:
4831 1107 699 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 2406 2908 3368 3620 1184
Size: 882 340 502 460 252 4402
Fragments arranged by size:
4402 882 502 460 340 252
AlwNI CAG_nnn'CTG
Cuts at: 2602 3259 3457 4581 6153 2602
Size: 657 198 1124 1572 3287
Fragments arranged by size:
3287 1572 1124 657 198
ApaI G_GGCC'C
Cuts at: 2513 2633 3273 3922 4026 2513
Size: 120 640 649 104 5325
Fragments arranged by size:
5325 649 640 120 104
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 4147 4373 4525 6634 1493
Size: 2654 226 152 2109 1697
Fragments arranged by size:
2654 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3745 3745
Size: 6838
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 3956 4163 1870
Size: 73 164 1849 207 4545
Fragments arranged by size:
4545 1849 207 164 73
BamHI G'GATC_C
Cuts at: 2697 4168 4535 2697
Size: 1471 367 5000
Fragments arranged by size:
5000 1471 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2564 3644 4159 5614
Size: 325 217 627 293 484 1080 515 1455
Cuts at: 5614 618
Size: 1842
Fragments arranged by size:
1842 1455 1080 627 515 484 325 293
217
BanII G_rGCy'C
Cuts at: 1391 2295 2513 2633 2652 3193 3273 3890 3922
Size: 904 218 120 19 541 80 617 32
Cuts at: 3922 4026 4157 1391
Size: 104 131 4072
Fragments arranged by size:
4072 904 617 541 218 131 120 104
80 32 19
BbeI G_GCGC'C
Cuts at: 3648 3648
Size: 6838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 2977 928
Size: 971 196 882 4789
Fragments arranged by size:
4789 971 882 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3455 3489 4091 4125 5029 5063 1903
Size: 34 1518 34 602 34 904 34 3678
Fragments arranged by size:
3678 1518 904 602 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 3959 5449
Size: 107 122 71 631 605 1126 1161 1490
Cuts at: 5449 136
Size: 1525
Fragments arranged by size:
1525 1490 1161 1126 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2703 6833 2703
Size: 4130 2708
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2172 2341 2357 2513 2633 3273 3922
Size: 255 127 169 16 156 120 640 649
Cuts at: 3922 4026 4899 6055 1790
Size: 104 873 1156 2573
Fragments arranged by size:
2573 1156 873 649 640 255 169 156
127 120 104 16
BmgBI CAC'GTC
Cuts at: 1318 2271 3171 1318
Size: 953 900 4985
Fragments arranged by size:
4985 953 900
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3667 4569 5527
Size: 416 53 83 399 24 2250 902 958
Cuts at: 5527 442
Size: 1753
Fragments arranged by size:
2250 1753 958 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3807 1056
Size: 2751 4087
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3982 3982
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 2954 3332 3971 4071 5498 1239
Size: 1070 645 378 639 100 1427 2579
Fragments arranged by size:
2579 1427 1070 645 639 378 100
Bpu10I CC'TnA_GC
Cuts at: 2603 2634 2603
Size: 31 6807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3392 4963 5828 6126 2333
Size: 1059 1571 865 298 3045
Fragments arranged by size:
3045 1571 1059 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3233 3766 5501 882
Size: 617 1734 533 1735 2219
Fragments arranged by size:
2219 1735 1734 617 533
BsaAI yAC'GTr
Cuts at: 493 1204 3501 4135 4629 493
Size: 711 2297 634 494 2702
Fragments arranged by size:
2702 2297 711 634 494
BsaBI GATnn'nnATC
Cuts at: 2702 3392 6832 2702
Size: 690 3440 2708
Fragments arranged by size:
3440 2708 690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3645 4645 5027
Size: 53 83 186 936 1179 933 1000 382
Cuts at: 5027 5662 275
Size: 635 1451
Fragments arranged by size:
1451 1179 1000 936 933 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5271 5669 5943 6090 6501 5271
Size: 398 274 147 411 5608
Fragments arranged by size:
5608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2564 2594 2750 2780 3053
Size: 30 165 30 1161 30 156 30 273
Cuts at: 3053 3083 3272 3302 4044 4074 1178
Size: 30 189 30 742 30 3942
Fragments arranged by size:
3942 1161 742 273 189 165 156 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 2771 3296 3521 3680 3794 3955 1186
Size: 1450 135 525 225 159 114 161 4069
Fragments arranged by size:
4069 1450 525 225 161 159 135 114
BseYI C'CCAG_C
Cuts at: 2061 2626 3046 3259 3698 3704 3926 3939 4034
Size: 565 420 213 439 6 222 13 95
Cuts at: 4034 4070 6041 6537 2061
Size: 36 1971 496 2362
Fragments arranged by size:
2362 1971 565 496 439 420 222 213
95 36 13 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 2615 2795 2804 3233 3587 3611
Size: 573 535 344 180 9 429 354 24
Cuts at: 3611 4131 4139 1163
Size: 520 8 3862
Fragments arranged by size:
3862 573 535 520 429 354 344 180
24 9 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2295 2652 2847 4899 4984 6055 2193
Size: 102 357 195 2052 85 1071 2976
Fragments arranged by size:
2976 2052 1071 357 195 102 85
BsiWI C'GTAC_G
Cuts at: 3501 3501
Size: 6838
BsmI GAATG_Cn'
Cuts at: 4349 4442 4349
Size: 93 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2172 2193 2295 2341 2357 2513
Size: 399 255 127 21 102 46 16 156
Cuts at: 2513 2633 2652 2847 3193 3273 3890 3922 4026
Size: 120 19 195 346 80 617 32 104
Cuts at: 4026 4157 4899 4984 6055 1391
Size: 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 399 346 255 195
156 131 127 120 104 102 85 80
46 32 21 19 16
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 3528 4727 6457 3528
Size: 1199 1730 3909
Fragments arranged by size:
3909 1730 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2212 2770 3434 3633 3639 3745 3832 4048
Size: 1368 558 664 199 6 106 87 216
Cuts at: 4048 4193 844
Size: 145 3489
Fragments arranged by size:
3489 1368 664 558 216 199 145 106
87 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 2371 2683 3316 4725 1394
Size: 429 395 153 312 633 1409 3507
Fragments arranged by size:
3507 1409 633 429 395 312 153
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2788 3899 5482 5669 1700
Size: 110 978 1111 1583 187 2869
Fragments arranged by size:
2869 1583 1111 978 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 2747 3745 1925
Size: 822 998 5018
Fragments arranged by size:
5018 998 822
BssSI C'ACGA_G
Cuts at: 2165 2232 4898 5910 2165
Size: 67 2666 1012 3093
Fragments arranged by size:
3093 2666 1012 67
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2449 2782 3997 794
Size: 1655 333 1215 3635
Fragments arranged by size:
3635 1655 1215 333
BstBI TT'CG_AA
Cuts at: 2007 5642 2007
Size: 3635 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstXI CCAn_nnnn'nTGG
Cuts at: 3757 4049 3757
Size: 292 6546
BstYI r'GATC_y
Cuts at: 1972 2598 2697 2703 2859 3195 3849 4168 4535
Size: 626 99 6 156 336 654 319 367
Cuts at: 4535 4920 4937 6389 6475 6487 6833 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 654 626 385 367 346 336
319 156 99 86 17 12 6
BstZ17I GTA'TAC
Cuts at: 3231 3231
Size: 6838
Bsu36I CC'TnA_GG
Cuts at: 2490 3945 2490
Size: 1455 5383
BtgI C'CryG_G
Cuts at: 513 1065 2274 2319 2657 3561 513
Size: 552 1209 45 338 904 3790
Fragments arranged by size:
3790 1209 904 552 338 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 2470 2699 4354 5148 5168 2341
Size: 129 229 1655 794 20 4011
Fragments arranged by size:
4011 1655 794 229 129 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3599 5845 809
Size: 507 2283 2246 1802
Fragments arranged by size:
2283 2246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2790 3636 4285
Size: 54 1425 50 275 18 960 846 649
Cuts at: 4285 5174 6803 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 960 889 846 649 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 3353 4768 1353
Size: 770 1230 1415 3423
Fragments arranged by size:
3423 1415 1230 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2650 2293
Size: 357 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2309 2660 2684 2954 3113 3332
Size: 393 511 166 351 24 270 159 219
Cuts at: 3332 3344 3971 4071 4901 5498 6285 6553 1239
Size: 12 627 100 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 627 597 511 393 351
270 268 219 166 159 100 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2879 3251 2879
Size: 372 6466
EcoO109I rG'GnC_Cy
Cuts at: 2224 2509 2510 2630 2938 3269 3307 3918 3919
Size: 285 1 120 308 331 38 611 1
Cuts at: 3919 4023 2224
Size: 104 5039
Fragments arranged by size:
5039 611 331 308 285 120 104 38
1 1
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 3382 2767
Size: 615 6223
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 3380 3648 5985 1678
Size: 1702 268 2337 2531
Fragments arranged by size:
2531 2337 1702 268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 2750 2782 2856
Size: 32 135 32 654 32 492 32 74
Cuts at: 2856 2888 3053 3085 3180 3212 3332 3364 4046
Size: 32 165 32 95 32 120 32 682
Cuts at: 4046 4078 5479 5511 5553 5585 1373
Size: 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 682 654 492 165 135 120
95 74 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KasI G'GCGC_C
Cuts at: 3644 3644
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3414 5952 6136 1305
Size: 2109 2538 184 2007
Fragments arranged by size:
2538 2109 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5016
Fragments arranged by size:
5016 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 2388 2534 2972 3005 3173 3488
Size: 648 1107 115 146 438 33 168 315
Cuts at: 3488 3602 4796 5155 5314 518
Size: 114 1194 359 159 2042
Fragments arranged by size:
2042 1194 1107 648 438 359 315 168
159 146 115 114 33
MspA1I CmG'CkG
Cuts at: 1606 2065 2659 2689 3304 3563 3681 3926 4254
Size: 459 594 30 615 259 118 245 328
Cuts at: 4254 4931 6079 6324 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 594 459 328 259
245 245 118 30
NaeI GCC'GGC
Cuts at: 2790 3901 2790
Size: 1111 5727
NarI GG'CG_CC
Cuts at: 3645 3645
Size: 6838
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6286
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NgoMIV G'CCGG_C
Cuts at: 2788 3899 2788
Size: 1111 5727
NheI G'CTAG_C
Cuts at: 1052 3803 1052
Size: 2751 4087
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2539 3205 4196 5741 1132
Size: 97 1310 666 991 1545 2229
Fragments arranged by size:
2229 1545 1310 991 666 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6838
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 3259 3657 1108
Size: 1179 972 398 4289
Fragments arranged by size:
4289 1179 972 398
PfoI T'CCnGG_A
Cuts at: 2931 3309 3369 3450 3771 2931
Size: 378 60 81 321 5998
Fragments arranged by size:
5998 378 321 81 60
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 3307 2224
Size: 1083 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspOMI G'GGCC_C
Cuts at: 2509 2629 3269 3918 4022 2509
Size: 120 640 649 104 5325
Fragments arranged by size:
5325 649 640 120 104
PstI C_TGCA'G
Cuts at: 830 2457 3147 3387 3522 3546 4190 830
Size: 1627 690 240 135 24 644 3478
Fragments arranged by size:
3478 1627 690 644 240 135 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2065 2065
Size: 6838
SacI G_AGCT'C
Cuts at: 2295 2652 2295
Size: 357 6481
SacII CC_GC'GG
Cuts at: 2660 3564 2660
Size: 904 5934
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SbfI CC_TGCA'GG
Cuts at: 3546 4190 3546
Size: 644 6194
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SexAI A'CCwGG_T
Cuts at: 2610 2610
Size: 6838
SfcI C'TryA_G
Cuts at: 826 1045 2453 3143 3383 3518 3542 4186 5321
Size: 219 1408 690 240 135 24 644 1135
Cuts at: 5321 6002 6193 826
Size: 681 191 1471
Fragments arranged by size:
1471 1408 1135 690 681 644 240 219
191 135 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2798 2798
Size: 6838
SfoI GGC'GCC
Cuts at: 3646 3646
Size: 6838
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3407 4942 5843 6105 820
Size: 197 926 405 1059 1535 901 262 1553
Fragments arranged by size:
1553 1535 1059 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 4135 4629 493
Size: 3642 494 2702
Fragments arranged by size:
3642 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 3858 3962 1517
Size: 2341 104 4393
Fragments arranged by size:
4393 2341 104
StyI C'CwwG_G
Cuts at: 513 1065 2499 2550 2742 2769 2838 2880 3276
Size: 552 1434 51 192 27 69 42 396
Cuts at: 3276 3868 4018 4277 513
Size: 592 150 259 3074
Fragments arranged by size:
3074 1434 592 552 396 259 192 150
69 51 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4865 4882 5035 5220 5665 325
Size: 1255 3285 17 153 185 445 1498
Fragments arranged by size:
3285 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3054 3669 5084
Size: 274 80 33 51 1443 1077 615 1415
Cuts at: 5084 6796 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1415 1077 615 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 3272 3304 3996 4028 4044 4076 6798
Size: 32 676 32 692 32 16 32 2722
Cuts at: 6798 6830 2564
Size: 32 2572
Fragments arranged by size:
2722 2572 692 676 32 32 32 32
32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 3839 1316
Size: 2523 4315
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3484 3484
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 2117 3362 4967 2117
Size: 1245 1605 3988
Fragments arranged by size:
3988 1605 1245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BsmBI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SapI SrfI SwaI