Restriction map for
pFN21ASDB0030
(Circular) (Six-base) MAPSORT of: pFN21ASDB0030.seq Check: 6636 from: 1 to: 9547
pFN21ASDB0030 9547 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5590 5590
Size: 9547
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4958 7357 278
Size: 53 83 186 936 3422 2399 2468
Fragments arranged by size:
3422 2468 2399 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 5897 5975 6314 6890 1937
Size: 346 3614 78 339 576 4594
Fragments arranged by size:
4594 3614 576 346 339 78
Acc65I G'GTAC_C
Cuts at: 5431 6868 5431
Size: 1437 8110
AclI AA'CG_TT
Cuts at: 1563 3874 7674 8047 1563
Size: 2311 3800 373 3063
Fragments arranged by size:
3800 3063 2311 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 3165 3366 4703 4787 5584 5762 6288
Size: 1158 375 201 1337 84 797 178 526
Cuts at: 6288 6416 7610 8994 9262 1632
Size: 128 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1337 1194 1158 797 526 375
268 201 178 128 84
AflII C'TTAA_G
Cuts at: 820 1017 3286 4814 820
Size: 197 2269 1528 5553
Fragments arranged by size:
5553 2269 1528 197
AflIII A'CryG_T
Cuts at: 1203 4494 5458 6048 6471 8446 9423 1203
Size: 3291 964 590 423 1975 977 1327
Fragments arranged by size:
3291 1975 1327 977 964 590 423
AgeI A'CCGG_T
Cuts at: 2745 3043 8378 2745
Size: 298 5335 3914
Fragments arranged by size:
5335 3914 298
AhdI GACnn_n'nnGTC
Cuts at: 8276 8276
Size: 9547
AleI CACnn'nnGTG
Cuts at: 1166 2513 3560 1166
Size: 1347 1047 7153
Fragments arranged by size:
7153 1347 1047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3746 5030 5851 1184
Size: 2562 1284 821 4880
Fragments arranged by size:
4880 2562 1284 821
AlwNI CAG_nnn'CTG
Cuts at: 2255 3056 3572 3716 4378 4550 5378 5848 5944
Size: 801 516 144 662 172 828 470 96
Cuts at: 5944 7290 8862 2255
Size: 1346 1572 2940
Fragments arranged by size:
2940 1572 1346 828 801 662 516 470
172 144 96
ApaI G_GGCC'C
Cuts at: 2212 2644 4092 2212
Size: 432 1448 7667
Fragments arranged by size:
7667 1448 432
ApaLI G'TGCA_C
Cuts at: 7604 8760 7604
Size: 1156 8391
ApoI r'AATT_y
Cuts at: 1493 2453 2586 3114 3535 3783 6257 6856 7082
Size: 960 133 528 421 248 2474 599 226
Cuts at: 7082 7234 9343 1493
Size: 152 2109 1697
Fragments arranged by size:
2474 2109 1697 960 599 528 421 248
226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 8101 160
Size: 872 7069 1606
Fragments arranged by size:
7069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9547
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2218 2235 3809 4704 6872 1870
Size: 73 152 123 17 1574 895 2168 4545
Fragments arranged by size:
4545 2168 1574 895 152 123 73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2384 2417 2384
Size: 33 9514
BamHI G'GATC_C
Cuts at: 2294 4000 6877 7244 2294
Size: 1706 2877 367 4597
Fragments arranged by size:
4597 2877 1706 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 3340 5014 5431 6100
Size: 325 217 627 684 869 1674 417 669
Cuts at: 6100 6868 8323 618
Size: 768 1455 1842
Fragments arranged by size:
1842 1674 1455 869 768 684 669 627
417 325 217
BanII G_rGCy'C
Cuts at: 1391 2212 2422 2644 3265 4092 4470 5738 6487
Size: 821 210 222 621 827 378 1268 749
Cuts at: 6487 6866 1391
Size: 379 4072
Fragments arranged by size:
4072 1268 827 821 749 621 379 378
222 210
BbeI G_GCGC'C
Cuts at: 2475 3344 2475
Size: 869 8678
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3783 4958 928
Size: 971 1884 1175 5517
Fragments arranged by size:
5517 1884 1175 971
BbvCI CC'TCA_GC
Cuts at: 6021 6021
Size: 9547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6556 6590 7738 7772 1903
Size: 34 4619 34 1148 34 3678
Fragments arranged by size:
4619 3678 1148 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6475 7439 8649 6475
Size: 964 1210 7373
Fragments arranged by size:
7373 1210 964
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9547
BfrBI ATG'CAT
Cuts at: 4792 4792
Size: 9547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 5650 6629
Size: 107 122 71 631 605 2454 1524 979
Cuts at: 6629 8158 136
Size: 1529 1525
Fragments arranged by size:
2454 1529 1525 1524 979 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2322 3148 9542 2322
Size: 826 6394 2327
Fragments arranged by size:
6394 2327 826
BlpI GC'TnA_GC
Cuts at: 6245 6753 6964 6245
Size: 508 211 8828
Fragments arranged by size:
8828 508 211
Bme1580I G_kGCm'C
Cuts at: 1790 2212 2644 3222 3735 4092 4527 6105 6355
Size: 422 432 578 513 357 435 1578 250
Cuts at: 6355 7608 8764 1790
Size: 1253 1156 2573
Fragments arranged by size:
2573 1578 1253 1156 578 513 435 432
422 357 250
BmgBI CAC'GTC
Cuts at: 1318 3740 3958 4987 5459 6703 1318
Size: 2422 218 1029 472 1244 4162
Fragments arranged by size:
4162 2422 1244 1029 472 218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2522 2846 2973
Size: 416 53 83 399 24 1105 324 127
Cuts at: 2973 3287 5723 7278 8236 442
Size: 314 2436 1555 958 1753
Fragments arranged by size:
2436 1753 1555 1105 958 416 399 324
314 127 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5670 1056
Size: 4614 4933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5869 5869
Size: 9547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 2970 3062 3351 5133 5325 5526 5924
Size: 1469 262 92 289 1782 192 201 398
Cuts at: 5924 6194 6404 6611 8207 1239
Size: 270 210 207 1596 2579
Fragments arranged by size:
2579 1782 1596 1469 398 289 270 262
210 207 201 192 92
Bpu10I CC'TnA_GC
Cuts at: 3399 4076 5344 6021 3399
Size: 677 1268 677 6925
Fragments arranged by size:
6925 1268 677 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2679 3543 4859 5500 7672 8537 8835 2679
Size: 864 1316 641 2172 865 298 3391
Fragments arranged by size:
3391 2172 1316 865 864 641 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2187 3621 3752 5502 5511 6136 8210
Size: 617 688 1434 131 1750 9 625 2074
Cuts at: 8210 882
Size: 2219
Fragments arranged by size:
2219 2074 1750 1434 688 625 617 131
9
BsaAI yAC'GTr
Cuts at: 493 1204 6049 6844 7338 493
Size: 711 4845 795 494 2702
Fragments arranged by size:
4845 2702 795 711 494
BsaBI GATnn'nnATC
Cuts at: 4278 9541 4278
Size: 5263 4284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2472 2711 3341 4678
Size: 53 83 186 936 939 239 630 1337
Cuts at: 4678 4955 7354 7736 8371 275
Size: 277 2399 382 635 1451
Fragments arranged by size:
2399 1451 1337 939 936 635 630 382
277 239 186 83 53
BsaWI w'CCGG_w
Cuts at: 2745 3043 7980 8378 8652 8799 9210 2745
Size: 298 4937 398 274 147 411 3082
Fragments arranged by size:
4937 3082 411 398 298 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 2618 2648 1178
Size: 30 165 30 918 30 267 30 8077
Fragments arranged by size:
8077 918 267 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 2988 3177 3323 3419 3495 3598 3878
Size: 1453 349 189 146 96 76 103 280
Cuts at: 3878 5485 5529 5630 5774 5777 6341 6380 6836
Size: 1607 44 101 144 3 564 39 456
Cuts at: 6836 6839 1186
Size: 3 3894
Fragments arranged by size:
3894 1607 1453 564 456 349 280 189
146 144 103 101 96 76 44 39
3 3
BseYI C'CCAG_C
Cuts at: 2211 2269 2332 2814 3193 3394 4250 5319 5386
Size: 58 63 482 379 201 856 1069 67
Cuts at: 5386 5640 5963 8750 9246 2211
Size: 254 323 2787 496 2512
Fragments arranged by size:
2787 2512 1069 856 496 482 379 323
254 201 67 63 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 2261 2268 2429 2516 5123 5486
Size: 573 511 14 7 161 87 2607 363
Cuts at: 5486 5861 5930 1163
Size: 375 69 4780
Fragments arranged by size:
4780 2607 573 511 375 363 161 87
69 14 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3883 6997 7758 8786 1540
Size: 275 186 1882 3114 761 1028 2301
Fragments arranged by size:
3114 2301 1882 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5094 7608 7693 8764 5094
Size: 2514 85 1071 5877
Fragments arranged by size:
5877 2514 1071 85
BsmI GAATG_Cn'
Cuts at: 6287 7058 7151 6287
Size: 771 93 8683
Fragments arranged by size:
8683 771 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2702 3580 2702
Size: 878 8669
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2212 2422 2644 3222 3265 3735 4092
Size: 399 422 210 222 578 43 470 357
Cuts at: 4092 4470 4527 5094 5738 6105 6355 6487 6866
Size: 378 57 567 644 367 250 132 379
Cuts at: 6866 7608 7693 8764 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 578 567 470 422
399 379 378 367 357 250 222 210
132 85 57 43
BspEI T'CCGG_A
Cuts at: 9210 9210
Size: 9547
BspHI T'CATG_A
Cuts at: 3118 6489 7436 9166 3118
Size: 3371 947 1730 3499
Fragments arranged by size:
3499 3371 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5590 6672 6902 844
Size: 4746 1082 230 3489
Fragments arranged by size:
4746 3489 1082 230
BsrBI CCG'CTC
Cuts at: 1394 1823 5530 5620 7434 1394
Size: 429 3707 90 1814 3507
Fragments arranged by size:
3707 3507 1814 429 90
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 3222 4188 5916 8042 8216 65
Size: 1183 1515 459 966 1728 2126 174 1396
Fragments arranged by size:
2126 1728 1515 1396 1183 966 459 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 2745 2772 3043 4129 5559 8191
Size: 110 300 635 27 271 1086 1430 2632
Cuts at: 8191 8378 1700
Size: 187 2869
Fragments arranged by size:
2869 2632 1430 1086 635 300 271 187
110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9547
BssSI C'ACGA_G
Cuts at: 5922 7607 8619 5922
Size: 1685 1012 6850
Fragments arranged by size:
6850 1685 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2255 2765 4987 5944 5953 794
Size: 1461 510 2222 957 9 4388
Fragments arranged by size:
4388 2222 1461 957 510 9
BstBI TT'CG_AA
Cuts at: 2007 8351 2007
Size: 6344 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9547
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 3094 3562 6281 2515
Size: 579 468 2719 5781
Fragments arranged by size:
5781 2719 579 468
BstYI r'GATC_y
Cuts at: 1972 2294 2322 2593 3148 4000 4993 5055 6177
Size: 322 28 271 555 852 993 62 1122
Cuts at: 6177 6877 7244 7629 7646 9098 9184 9196 9542
Size: 700 367 385 17 1452 86 12 346
Cuts at: 9542 1972
Size: 1977
Fragments arranged by size:
1977 1452 1122 993 852 700 555 385
367 346 322 271 86 62 28 17
12
BstZ17I GTA'TAC
Cuts at: 6315 6315
Size: 9547
Bsu36I CC'TnA_GG
Cuts at: 3243 3744 5646 3243
Size: 501 1902 7144
Fragments arranged by size:
7144 1902 501
BtgI C'CryG_G
Cuts at: 513 1065 4084 4123 5167 5551 5689 6105 513
Size: 552 3019 39 1044 384 138 416 3955
Fragments arranged by size:
3955 3019 1044 552 416 384 138 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8808
Fragments arranged by size:
8808 683 56
BtsI GCAGTG_nn'
Cuts at: 2230 2646 3730 3986 5131 5917 6555 7063 7857
Size: 416 1084 256 1145 786 638 508 794
Cuts at: 7857 7877 2230
Size: 20 3900
Fragments arranged by size:
3900 1145 1084 794 786 638 508 416
256 20
ClaI AT'CG_AT
Cuts at: 1554 4579 5277 1554
Size: 3025 698 5824
Fragments arranged by size:
5824 3025 698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2365 2400 529
Size: 35 1801 35 7676
Fragments arranged by size:
7676 1801 35 35
DraI TTT'AAA
Cuts at: 6851 6851
Size: 9547
DraIII CAC_nnn'GTG
Cuts at: 3109 3109
Size: 9547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4802 8554 809
Size: 507 3486 3752 1802
Fragments arranged by size:
3752 3486 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2735 2774 3992
Size: 54 1425 50 275 18 905 39 1218
Cuts at: 3992 4063 4401 5115 6343 6994 7883 9512 8
Size: 71 338 714 1228 651 889 1629 43
Fragments arranged by size:
1629 1425 1228 1218 905 889 714 651
338 275 71 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 6994 1537
Size: 275 5182 4090
Fragments arranged by size:
5182 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3423 4683 4692 4824 5873 7477 1353
Size: 2070 1260 9 132 1049 1604 3423
Fragments arranged by size:
3423 2070 1604 1260 1049 132 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8134 8508 8654 387
Size: 1064 138 6545 374 146 1280
Fragments arranged by size:
6545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2708 2790 2970 3062 3165 3351 3366
Size: 393 1076 82 180 92 103 186 15
Cuts at: 3366 4703 4787 5133 5325 5526 5584 5762 5924
Size: 1337 84 346 192 201 58 178 162
Cuts at: 5924 6194 6288 6404 6416 6611 7610 8207 8994
Size: 270 94 116 12 195 999 597 787
Cuts at: 8994 9262 1239
Size: 268 1524
Fragments arranged by size:
1524 1337 1076 999 787 597 393 346
270 268 201 195 192 186 180 178
162 116 103 94 92 84 82 58
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3690 5675 6323 3690
Size: 1985 648 6914
Fragments arranged by size:
6914 1985 648
EcoO109I rG'GnC_Cy
Cuts at: 2048 2346 2460 2640 4089 4142 4163 4318 5036
Size: 298 114 180 1449 53 21 155 718
Cuts at: 5036 5153 6206 6631 6745 2048
Size: 117 1053 425 114 4850
Fragments arranged by size:
4850 1449 1053 718 425 298 180 155
117 114 114 53 21
EcoRI G'AATT_C
Cuts at: 2586 6856 7234 2586
Size: 4270 378 4899
Fragments arranged by size:
4899 4270 378
EcoRV GAT'ATC
Cuts at: 2617 2617
Size: 9547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 4853 5614 6260 3050
Size: 1803 761 646 6337
Fragments arranged by size:
6337 1803 761 646
FspI TGC'GCA
Cuts at: 5657 8053 5657
Size: 2396 7151
HaeII r_GCGC'y
Cuts at: 1678 2111 2475 3344 4153 4517 4726 6617 8694
Size: 433 364 869 809 364 209 1891 2077
Cuts at: 8694 1678
Size: 2531
Fragments arranged by size:
2531 2077 1891 869 809 433 364 364
209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 2732 2764 3152
Size: 32 135 32 1046 32 82 32 388
Cuts at: 3152 3184 4817 4849 5079 5111 5354 5386 5762
Size: 32 1633 32 230 32 243 32 376
Cuts at: 5762 5794 6812 6844 8188 8220 8262 8294 1373
Size: 32 1018 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1633 1344 1046 1018 388 376 243
230 135 82 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4776 6891 7137 669
Size: 988 281 2838 2115 246 3079
Fragments arranged by size:
3079 2838 2115 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9547
HpaI GTT'AAC
Cuts at: 4776 7137 4776
Size: 2361 7186
KasI G'GCGC_C
Cuts at: 2471 3340 2471
Size: 869 8678
KpnI G_GTAC'C
Cuts at: 5435 6872 5435
Size: 1437 8110
MfeI C'AATT_G
Cuts at: 5008 7146 5008
Size: 2138 7409
MluI A'CGCG_T
Cuts at: 9423 9423
Size: 9547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2858 3254 3604 6312 6492 8661 8845 1305
Size: 1553 396 350 2708 180 2169 184 2007
Fragments arranged by size:
2708 2169 2007 1553 396 350 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4403 5117 6345 10
Size: 54 1425 343 2571 714 1228 3212
Fragments arranged by size:
3212 2571 1425 1228 714 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3560 3951 4797 5099 5811 7505
Size: 648 1347 1047 391 846 302 712 1694
Cuts at: 7505 7864 8023 518
Size: 359 159 2042
Fragments arranged by size:
2042 1694 1347 1047 846 712 648 391
359 302 159
MspA1I CmG'CkG
Cuts at: 1606 2273 3278 4378 4399 4503 5337 5941 5953
Size: 667 1005 1100 21 104 834 604 12
Cuts at: 5953 6382 6418 6752 6963 7640 8788 9033 1606
Size: 429 36 334 211 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 1005 834 677 667 604
429 334 245 211 104 36 21 12
NaeI GCC'GGC
Cuts at: 4131 4131
Size: 9547
NarI GG'CG_CC
Cuts at: 2472 3341 2472
Size: 869 8678
NcoI C'CATG_G
Cuts at: 513 1065 6105 513
Size: 552 5040 3955
Fragments arranged by size:
5040 3955 552
NdeI CA'TA_TG
Cuts at: 387 5931 387
Size: 5544 4003
NgoMIV G'CCGG_C
Cuts at: 4129 4129
Size: 9547
NheI G'CTAG_C
Cuts at: 1052 5666 1052
Size: 4614 4933
NotI GC'GGCC_GC
Cuts at: 6994 6994
Size: 9547
NsiI A_TGCA'T
Cuts at: 4794 4794
Size: 9547
NspI r_CATG'y
Cuts at: 1132 1229 4498 6905 8450 1132
Size: 97 3269 2407 1545 2229
Fragments arranged by size:
3269 2407 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2341 6319 1348
Size: 993 3978 4576
Fragments arranged by size:
4576 3978 993
PciI A'CATG_T
Cuts at: 4494 8446 4494
Size: 3952 5595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4100 1108
Size: 2992 6555
PmeI GTTT'AAAC
Cuts at: 6851 6851
Size: 9547
PmlI CAC'GTG
Cuts at: 6049 6049
Size: 9547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7616 7648 9129 9161 7616
Size: 32 1481 32 8002
Fragments arranged by size:
8002 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2460 4142 4163 4318 5036 6206 6745 2460
Size: 1682 21 155 718 1170 539 5262
Fragments arranged by size:
5262 1682 1170 718 539 155 21
PshAI GACnn'nnGTC
Cuts at: 1613 5133 6497 1613
Size: 3520 1364 4663
Fragments arranged by size:
4663 3520 1364
PsiI TTA'TAA
Cuts at: 7117 7117
Size: 9547
PspOMI G'GGCC_C
Cuts at: 2208 2640 4088 2208
Size: 432 1448 7667
Fragments arranged by size:
7667 1448 432
PstI C_TGCA'G
Cuts at: 830 2190 3694 4983 6090 6579 6899 830
Size: 1360 1504 1289 1107 489 320 3478
Fragments arranged by size:
3478 1504 1360 1289 1107 489 320
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9547
PvuII CAG'CTG
Cuts at: 2273 3278 4378 4399 5941 5953 6382 2273
Size: 1005 1100 21 1542 12 429 5438
Fragments arranged by size:
5438 1542 1100 1005 429 21 12
SalI G'TCGA_C
Cuts at: 1936 6889 1936
Size: 4953 4594
SanDI GG'GwC_CC
Cuts at: 4142 4163 5036 4142
Size: 21 873 8653
Fragments arranged by size:
8653 873 21
SapI GCTCTTCn'nnn_
Cuts at: 4683 5873 4683
Size: 1190 8357
SbfI CC_TGCA'GG
Cuts at: 3694 6899 3694
Size: 3205 6342
ScaI AGT'ACT
Cuts at: 5398 5987 7795 9507 5398
Size: 589 1808 1712 5438
Fragments arranged by size:
5438 1808 1712 589
SfcI C'TryA_G
Cuts at: 826 1045 2186 2448 2931 3690 4979 6086 6575
Size: 219 1141 262 483 759 1289 1107 489
Cuts at: 6575 6608 6724 6895 8030 8711 8902 826
Size: 33 116 171 1135 681 191 1471
Fragments arranged by size:
1471 1289 1141 1135 1107 759 681 489
483 262 219 191 171 116 33
SfoI GGC'GCC
Cuts at: 2473 3342 2473
Size: 869 8678
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9547
SgrAI Cr'CCGG_yG
Cuts at: 1700 2110 1700
Size: 410 9137
SmaI CCC'GGG
Cuts at: 1872 2097 3811 6874 1872
Size: 225 1714 3063 4545
Fragments arranged by size:
4545 3063 1714 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2694 3286 3558 4814 4838 5479
Size: 197 926 751 592 272 1256 24 641
Cuts at: 5479 7651 8552 8814 820
Size: 2172 901 262 1553
Fragments arranged by size:
2172 1553 1256 926 901 751 641 592
272 262 197 24
SnaBI TAC'GTA
Cuts at: 493 6844 7338 493
Size: 6351 494 2702
Fragments arranged by size:
6351 2702 494
SpeI A'CTAG_T
Cuts at: 152 5393 152
Size: 5241 4306
SphI G_CATG'C
Cuts at: 1132 6905 1132
Size: 5773 3774
SspI AAT'ATT
Cuts at: 5 52 7471 5
Size: 47 7419 2081
Fragments arranged by size:
7419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9547
StyI C'CwwG_G
Cuts at: 513 1065 2488 2602 3318 4060 4094 5118 5781
Size: 552 1423 114 716 742 34 1024 663
Cuts at: 5781 5992 6105 6986 513
Size: 211 113 881 3074
Fragments arranged by size:
3074 1423 1024 881 742 716 663 552
211 114 113 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2674 4223 5002 5526 6094 7574 7591
Size: 1255 1094 1549 779 524 568 1480 17
Cuts at: 7591 7744 7929 8374 325
Size: 153 185 445 1498
Fragments arranged by size:
1549 1498 1480 1255 1094 779 568 524
445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5396 5985 7793
Size: 274 80 33 51 1443 3419 589 1808
Cuts at: 7793 9505 96
Size: 1712 138
Fragments arranged by size:
3419 1808 1712 1443 589 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9507 9539 9507
Size: 32 9515
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9547
XbaI T'CTAG_A
Cuts at: 6883 6883
Size: 9547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4477 4903 4477
Size: 426 9121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9547
XmaI C'CCGG_G
Cuts at: 1870 2095 3809 6872 1870
Size: 225 1714 3063 4545
Fragments arranged by size:
4545 3063 1714 225
XmnI GAAnn'nnTTC
Cuts at: 5408 7676 5408
Size: 2268 7279
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4956 7355 276
Size: 53 83 186 936 3422 2399 2468
Fragments arranged by size:
3422 2468 2399 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BsiWI EcoICRI FseI FspAI
NruI PacI PfoI PspXI PsrI RsrII SacI SacII
SexAI SfiI SrfI SwaI