Restriction map for
pFN21ASDB0029
(Circular) (Six-base) MAPSORT of: pFN21ASDB0029.seq Check: 5822 from: 1 to: 6439
pFN21ASDB0029 6439 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2306 2588 2306
Size: 282 6157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4249 278
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3456 3782 1937
Size: 1519 326 4594
Fragments arranged by size:
4594 1519 326
Acc65I G'GTAC_C
Cuts at: 3760 3760
Size: 6439
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3013 4502 5886 6154 1632
Size: 1381 1489 1384 268 1917
Fragments arranged by size:
1917 1489 1384 1381 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 5338 6315 1203
Size: 4135 977 1327
Fragments arranged by size:
4135 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 5168 5168
Size: 6439
AleI CACnn'nnGTG
Cuts at: 1166 3653 1166
Size: 2487 3952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3395 1184
Size: 2211 4228
AlwNI CAG_nnn'CTG
Cuts at: 3269 3575 4182 5754 3269
Size: 306 607 1572 3954
Fragments arranged by size:
3954 1572 607 306
ApaLI G'TGCA_C
Cuts at: 4496 5652 4496
Size: 1156 5283
ApoI r'AATT_y
Cuts at: 1493 3748 3974 4126 6235 1493
Size: 2255 226 152 2109 1697
Fragments arranged by size:
2255 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 3579 3764 1870
Size: 73 1636 185 4545
Fragments arranged by size:
4545 1636 185 73
BamHI G'GATC_C
Cuts at: 3259 3769 4136 3259
Size: 510 367 5562
Fragments arranged by size:
5562 510 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2321 2347 3083 3560 3647
Size: 325 217 627 534 26 736 477 87
Cuts at: 3647 3694 3760 5215 618
Size: 47 66 1455 1842
Fragments arranged by size:
1842 1455 736 627 534 477 325 217
87 66 47 26
BanII G_rGCy'C
Cuts at: 1391 2565 2856 2948 3349 3587 3758 1391
Size: 1174 291 92 401 238 171 4072
Fragments arranged by size:
4072 1174 401 291 238 171 92
BbeI G_GCGC'C
Cuts at: 3698 3698
Size: 6439
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 928
Size: 971 296 5172
Fragments arranged by size:
5172 971 296
BbvCI CC'TCA_GC
Cuts at: 2721 2721
Size: 6439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 4630 4664 1903
Size: 34 1733 34 926 34 3678
Fragments arranged by size:
3678 1733 926 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3216 4331 5541 3216
Size: 1115 1210 4114
Fragments arranged by size:
4114 1210 1115
BclI T'GATC_A
Cuts at: 1548 2104 2331 1548
Size: 556 227 5656
Fragments arranged by size:
5656 556 227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2685 2915 3645
Size: 107 122 71 631 605 1013 230 730
Cuts at: 3645 5050 136
Size: 1405 1525
Fragments arranged by size:
1525 1405 1013 730 631 605 230 122
107 71
BglII A'GATC_T
Cuts at: 2571 3192 6434 2571
Size: 621 3242 2576
Fragments arranged by size:
3242 2576 621
BlpI GC'TnA_GC
Cuts at: 3673 3856 3673
Size: 183 6256
Bme1580I G_kGCm'C
Cuts at: 1790 3019 3086 4500 5656 1790
Size: 1229 67 1414 1156 2573
Fragments arranged by size:
2573 1414 1229 1156 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2914 4170 5128
Size: 416 53 83 399 24 1497 1256 958
Cuts at: 5128 442
Size: 1753
Fragments arranged by size:
1753 1497 1256 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2244 5099 1239
Size: 960 45 2855 2579
Fragments arranged by size:
2855 2579 960 45
Bpu10I CC'TnA_GC
Cuts at: 2311 2352 2721 2942 2311
Size: 41 369 221 5808
Fragments arranged by size:
5808 369 221 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2592 4564 5429 5727 2592
Size: 1972 865 298 3304
Fragments arranged by size:
3304 1972 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5102 882
Size: 617 3603 2219
Fragments arranged by size:
3603 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3403 3736 4230 493
Size: 711 2199 333 494 2702
Fragments arranged by size:
2702 2199 711 494 333
BsaBI GATnn'nnATC
Cuts at: 2216 2541 6433 2216
Size: 325 3892 2222
Fragments arranged by size:
3892 2222 325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3695 4246 4628 5263
Size: 53 83 186 936 2162 551 382 635
Cuts at: 5263 275
Size: 1451
Fragments arranged by size:
2162 1451 936 635 551 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2962 4872 5270 5544 5691 6102 2962
Size: 1910 398 274 147 411 3299
Fragments arranged by size:
3299 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2960 2990 3092 3122 1178
Size: 30 165 30 1557 30 102 30 4495
Fragments arranged by size:
4495 1557 165 102 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 3113 3339 1186
Size: 1010 917 226 4286
Fragments arranged by size:
4286 1010 917 226
BseYI C'CCAG_C
Cuts at: 2593 5642 6138 2593
Size: 3049 496 2894
Fragments arranged by size:
3049 2894 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 2820 3007 3383 1163
Size: 573 762 322 187 376 4219
Fragments arranged by size:
4219 762 573 376 322 187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3599 3709 3889 4650 5678 1540
Size: 275 186 1598 110 180 761 1028 2301
Fragments arranged by size:
2301 1598 1028 761 275 186 180 110
BsiHKAI G_wGCw'C
Cuts at: 2565 4500 4585 5656 2565
Size: 1935 85 1071 3348
Fragments arranged by size:
3348 1935 1071 85
BsmI GAATG_Cn'
Cuts at: 3950 4043 3950
Size: 93 6346
BsmBI CGTCTCn'nnnn_
Cuts at: 2075 3286 2075
Size: 1211 5228
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2565 2856 2948 3019 3086 3349 3587
Size: 399 775 291 92 71 67 263 238
Cuts at: 3587 3758 4500 4585 5656 1391
Size: 171 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 399 291 263 238
171 92 85 71 67
BspEI T'CCGG_A
Cuts at: 2962 6102 2962
Size: 3140 3299
BspHI T'CATG_A
Cuts at: 2787 4328 6058 2787
Size: 1541 1730 3168
Fragments arranged by size:
3168 1730 1541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 2588 3405 3501 3794 844
Size: 1462 282 817 96 293 3489
Fragments arranged by size:
3489 1462 817 293 282 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3103 4326 1394
Size: 429 1280 1223 3507
Fragments arranged by size:
3507 1280 1223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 2634 2745 4934 5108 65
Size: 1183 1353 33 111 2189 174 1396
Fragments arranged by size:
2189 1396 1353 1183 174 111 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 5083 5270 1700
Size: 110 3273 187 2869
Fragments arranged by size:
3273 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6439
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6439
BssSI C'ACGA_G
Cuts at: 2059 4499 5511 2059
Size: 2440 1012 2987
Fragments arranged by size:
2987 2440 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2476 3575 794
Size: 1682 1099 3658
Fragments arranged by size:
3658 1682 1099
BstBI TT'CG_AA
Cuts at: 2007 5243 2007
Size: 3236 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6439
BstXI CCAn_nnnn'nTGG
Cuts at: 3094 3416 3094
Size: 322 6117
BstYI r'GATC_y
Cuts at: 1972 2571 2995 3192 3259 3769 4136 4521 4538
Size: 599 424 197 67 510 367 385 17
Cuts at: 4538 5990 6076 6088 6434 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 599 510 424 385 367 346
197 86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 3078 3078
Size: 6439
BtgI C'CryG_G
Cuts at: 513 1065 2051 513
Size: 552 986 4901
Fragments arranged by size:
4901 986 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5700
Fragments arranged by size:
5700 683 56
BtsI GCAGTG_nn'
Cuts at: 2859 3019 3033 3955 4749 4769 2859
Size: 160 14 922 794 20 4529
Fragments arranged by size:
4529 922 794 160 20 14
ClaI AT'CG_AT
Cuts at: 1554 2232 1554
Size: 678 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6404
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 809
Size: 507 4130 1802
Fragments arranged by size:
4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2985 3132 3418
Size: 54 1425 50 275 18 1155 147 286
Cuts at: 3418 3596 3706 3886 4775 6404 8
Size: 178 110 180 889 1629 43
Fragments arranged by size:
1629 1425 1155 889 286 275 180 178
147 110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3596 3706 3886 1537
Size: 275 1784 110 180 4090
Fragments arranged by size:
4090 1784 275 180 110
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2703 4369 1353
Size: 1052 298 1666 3423
Fragments arranged by size:
3423 1666 1052 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5026 5400 5546 387
Size: 1064 138 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2563 2563
Size: 6439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2244 3013 4502 5099 5886 6154
Size: 393 567 45 769 1489 597 787 268
Cuts at: 6154 1239
Size: 1524
Fragments arranged by size:
1524 1489 787 769 597 567 393 268
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2372 2489 2078
Size: 294 117 6028
Fragments arranged by size:
6028 294 117
EcoRI G'AATT_C
Cuts at: 3748 4126 3748
Size: 378 6061
EcoRV GAT'ATC
Cuts at: 2632 2632
Size: 6439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 3079 2326
Size: 753 5686
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 3698 5586 1678
Size: 2020 1888 2531
Fragments arranged by size:
2531 2020 1888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2076 2085 2093 2108
Size: 32 135 32 481 23 9 8 15
Cuts at: 2108 2125 2345 2377 2798 2830 3092 3124 5080
Size: 17 220 32 421 32 262 32 1956
Cuts at: 5080 5112 5154 5186 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1956 481 421 262 220 135 42
32 32 32 32 32 32 32 23
17 15 9 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3783 4029 669
Size: 988 281 568 1277 246 3079
Fragments arranged by size:
3079 1277 988 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6439
HpaI GTT'AAC
Cuts at: 2506 4029 2506
Size: 1523 4916
KasI G'GCGC_C
Cuts at: 3694 3694
Size: 6439
KpnI G_GTAC'C
Cuts at: 3764 3764
Size: 6439
MfeI C'AATT_G
Cuts at: 2761 3048 4038 2761
Size: 287 990 5162
Fragments arranged by size:
5162 990 287
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 3471 5553 5737 1305
Size: 1335 831 2082 184 2007
Fragments arranged by size:
2082 2007 1335 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2987 3134 10
Size: 54 1425 343 1155 147 3315
Fragments arranged by size:
3315 1425 1155 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2454 3653 4397 4756 4915 518
Size: 648 1161 127 1199 744 359 159 2042
Fragments arranged by size:
2042 1199 1161 744 648 359 159 127
MspA1I CmG'CkG
Cuts at: 1606 2053 2804 2991 3855 4532 5680 5925 1606
Size: 447 751 187 864 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 751 677 447 245 187
NarI GG'CG_CC
Cuts at: 3695 3695
Size: 6439
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5887
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 2438 2659 3500 3517 3797 5342 1132
Size: 97 1209 221 841 17 280 1545 2229
Fragments arranged by size:
2229 1545 1209 841 280 221 97 17
PasI CC'CwG_GG
Cuts at: 1348 3409 1348
Size: 2061 4378
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3163 1108
Size: 2055 4384
PfoI T'CCnGG_A
Cuts at: 2176 2176
Size: 6439
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PmlI CAC'GTG
Cuts at: 3403 3403
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 4508 4540 6021 6053 2714
Size: 32 1762 32 1481 32 3100
Fragments arranged by size:
3100 1762 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6439
PsiI TTA'TAA
Cuts at: 2532 4009 2532
Size: 1477 4962
PstI C_TGCA'G
Cuts at: 830 3003 3437 3791 830
Size: 2173 434 354 3478
Fragments arranged by size:
3478 2173 434 354
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 2804 2804
Size: 6439
SacI G_AGCT'C
Cuts at: 2565 2565
Size: 6439
SacII CC_GC'GG
Cuts at: 2054 2054
Size: 6439
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SapI GCTCTTCn'nnn_
Cuts at: 2703 2703
Size: 6439
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SfcI C'TryA_G
Cuts at: 826 1045 2999 3433 3787 4922 5603 5794 826
Size: 219 1954 434 354 1135 681 191 1471
Fragments arranged by size:
1954 1471 1135 681 434 354 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2685 2685
Size: 6439
SfoI GGC'GCC
Cuts at: 3696 3696
Size: 6439
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 3766 1872
Size: 1894 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2607 4543 5444 5706 820
Size: 197 926 664 1936 901 262 1553
Fragments arranged by size:
1936 1553 926 901 664 262 197
SnaBI TAC'GTA
Cuts at: 493 3736 4230 493
Size: 3243 494 2702
Fragments arranged by size:
3243 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3500 3797 1132
Size: 2368 297 3774
Fragments arranged by size:
3774 2368 297
SspI AAT'ATT
Cuts at: 5 52 4363 5
Size: 47 4311 2081
Fragments arranged by size:
4311 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 1517
Size: 861 5578
StyI C'CwwG_G
Cuts at: 513 1065 2032 2293 2910 3506 3878 513
Size: 552 967 261 617 596 372 3074
Fragments arranged by size:
3074 967 617 596 552 372 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 3665 4466 4483 4636 4821 5266
Size: 1255 882 1203 801 17 153 185 445
Cuts at: 5266 325
Size: 1498
Fragments arranged by size:
1498 1255 1203 882 801 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2692 3312 4685
Size: 274 80 33 51 1443 715 620 1373
Cuts at: 4685 6397 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1373 715 620 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2047 2079 3058 3090 6399 6431 2047
Size: 32 979 32 3309 32 2055
Fragments arranged by size:
3309 2055 979 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2462 3248 3452 1316
Size: 1146 786 204 4303
Fragments arranged by size:
4303 1146 786 204
XbaI T'CTAG_A
Cuts at: 3775 3775
Size: 6439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 3047 3142 2209
Size: 838 95 5506
Fragments arranged by size:
5506 838 95
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6439
XmaI C'CCGG_G
Cuts at: 1870 3764 1870
Size: 1894 4545
XmnI GAAnn'nnTTC
Cuts at: 4568 4568
Size: 6439
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4247 276
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoO109I FseI FspAI NaeI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SanDI SexAI SrfI SwaI