Restriction map for
pFN21ASDB0028
(Circular) (Six-base) MAPSORT of: pFN21ASDB0028.seq Check: 3046 from: 1 to: 6394
pFN21ASDB0028 6394 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3737 1937
Size: 1800 4594
Acc65I G'GTAC_C
Cuts at: 3715 3715
Size: 6394
AclI AA'CG_TT
Cuts at: 1563 3263 4521 4894 1563
Size: 1700 1258 373 3063
Fragments arranged by size:
3063 1700 1258 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 4457 5841 6109 1632
Size: 686 2139 1384 268 1917
Fragments arranged by size:
2139 1917 1384 686 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 2775 5293 6270 1203
Size: 1572 2518 977 1327
Fragments arranged by size:
2518 1572 1327 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2361 1184
Size: 1177 5217
AlwNI CAG_nnn'CTG
Cuts at: 4137 5709 4137
Size: 1572 4822
ApaI G_GGCC'C
Cuts at: 2332 2332
Size: 6394
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 3424 3703 3929 4081 6190 1493
Size: 1931 279 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 3719 1870
Size: 73 244 1532 4545
Fragments arranged by size:
4545 1532 244 73
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2993 3715 5170 618
Size: 325 217 627 1206 722 1455 1842
Fragments arranged by size:
1842 1455 1206 722 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2332 3031 3494 3642 3654 3713 1391
Size: 941 699 463 148 12 59 4072
Fragments arranged by size:
4072 941 699 463 148 59 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 928
Size: 971 428 4995
Fragments arranged by size:
4995 971 428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4585 4619 1903
Size: 34 2648 34 3678
Fragments arranged by size:
3678 2648 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3379 4286 5496 3379
Size: 907 1210 4277
Fragments arranged by size:
4277 1210 907
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2459 5005 136
Size: 107 122 71 631 605 787 2546 1525
Fragments arranged by size:
2546 1525 787 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2488 6389 2488
Size: 3901 2493
BlpI GC'TnA_GC
Cuts at: 3030 3811 3030
Size: 781 5613
Bme1580I G_kGCm'C
Cuts at: 1790 2332 2998 3303 4455 5611 1790
Size: 542 666 305 1152 1156 2573
Fragments arranged by size:
2573 1156 1152 666 542 305
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3412 3636 3648
Size: 416 53 83 399 24 1995 224 12
Cuts at: 3648 4125 5083 442
Size: 477 958 1753
Fragments arranged by size:
1995 1753 958 477 416 399 224 83
53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2805 3337 5054 1239
Size: 823 743 532 1717 2579
Fragments arranged by size:
2579 1717 823 743 532
Bpu10I CC'TnA_GC
Cuts at: 3012 3012
Size: 6394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2503 4519 5384 5682 2210
Size: 293 2016 865 298 2922
Fragments arranged by size:
2922 2016 865 298 293
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 5057 882
Size: 617 1563 1995 2219
Fragments arranged by size:
2219 1995 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 3691 4185 493
Size: 711 2487 494 2702
Fragments arranged by size:
2702 2487 711 494
BsaBI GATnn'nnATC
Cuts at: 6388 6388
Size: 6394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 4583 5218 275
Size: 53 83 186 936 2668 382 635 1451
Fragments arranged by size:
2668 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2142 4827 5225 5499 5646 6057 2142
Size: 2685 398 274 147 411 2479
Fragments arranged by size:
2685 2479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3308 3338 1178
Size: 30 165 30 1905 30 4234
Fragments arranged by size:
4234 1905 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6394
BseYI C'CCAG_C
Cuts at: 2997 3285 3475 3569 5597 6093 2997
Size: 288 190 94 2028 496 3298
Fragments arranged by size:
3298 2028 496 288 190 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2889 1163
Size: 573 1153 4668
Fragments arranged by size:
4668 1153 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3844 4605 5633 1540
Size: 275 186 1843 761 1028 2301
Fragments arranged by size:
2301 1843 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3494 4455 4540 5611 3494
Size: 961 85 1071 4277
Fragments arranged by size:
4277 1071 961 85
BsmI GAATG_Cn'
Cuts at: 3905 3998 3905
Size: 93 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2998 3031 3303 3494 3642 3654
Size: 399 542 666 33 272 191 148 12
Cuts at: 3654 3713 4455 4540 5611 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 542 399 272 191
148 85 59 33 12
BspEI T'CCGG_A
Cuts at: 6057 6057
Size: 6394
BspHI T'CATG_A
Cuts at: 2353 4283 6013 2353
Size: 1930 1730 2734
Fragments arranged by size:
2734 1930 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3749 844
Size: 2905 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4281 1394
Size: 429 2458 3507
Fragments arranged by size:
3507 2458 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 2934 4889 5063 65
Size: 1183 1119 567 1955 174 1396
Fragments arranged by size:
1955 1396 1183 1119 567 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3680 5038 5225 1700
Size: 110 1870 1358 187 2869
Fragments arranged by size:
2869 1870 1358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6394
BssSI C'ACGA_G
Cuts at: 2642 3199 4454 5466 2642
Size: 557 1255 1012 3570
Fragments arranged by size:
3570 1255 1012 557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3518 794
Size: 2724 3670
BstBI TT'CG_AA
Cuts at: 2007 5198 2007
Size: 3191 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6394
BstYI r'GATC_y
Cuts at: 1972 2488 2605 3724 4091 4476 4493 5945 6031
Size: 516 117 1119 367 385 17 1452 86
Cuts at: 6031 6043 6389 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1119 516 385 367 346 117
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2914 2914
Size: 6394
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3022 487
Size: 683 56 1796 3859
Fragments arranged by size:
3859 1796 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2564 2898 3910 4704 4724 2107
Size: 457 334 1012 794 20 3777
Fragments arranged by size:
3777 1012 794 457 334 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6359
DraI TTT'AAA
Cuts at: 2128 3698 2128
Size: 1570 4824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5401 809
Size: 507 4085 1802
Fragments arranged by size:
4085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 3083 3093
Size: 54 1425 50 275 18 609 644 10
Cuts at: 3093 3841 4730 6359 8
Size: 748 889 1629 43
Fragments arranged by size:
1629 1425 889 748 644 609 275 54
50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3841 1537
Size: 275 2029 4090
Fragments arranged by size:
4090 2029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 3546 3576 4324 1353
Size: 1233 960 30 748 3423
Fragments arranged by size:
3423 1233 960 748 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3096 3282 4981 5355 5501 387
Size: 1064 138 1507 186 1699 374 146 1280
Fragments arranged by size:
1699 1507 1280 1064 374 186 146 138
EcoICRI GAG'CTC
Cuts at: 3492 3492
Size: 6394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2318 2805 3337 4457 5054 5841
Size: 393 430 256 487 532 1120 597 787
Cuts at: 5841 6109 1239
Size: 268 1524
Fragments arranged by size:
1524 1120 787 597 532 487 430 393
268 256
EcoO109I rG'GnC_Cy
Cuts at: 2328 2329 3167 2328
Size: 1 838 5555
Fragments arranged by size:
5555 838 1
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 2067 2247 2937 5541 1678
Size: 389 180 690 2604 2531
Fragments arranged by size:
2604 2531 690 389 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 3308 3340 5035
Size: 32 135 32 1211 32 493 32 1695
Cuts at: 5035 5067 5109 5141 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 1211 493 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 2735 3738 3984 669
Size: 988 281 90 707 1003 246 3079
Fragments arranged by size:
3079 1003 988 707 281 246 90
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 3719 3719
Size: 6394
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2998 3494 3642 5508 5692 1305
Size: 1693 496 148 1866 184 2007
Fragments arranged by size:
2007 1866 1693 496 184 148
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 3085 10
Size: 54 1425 343 609 644 3319
Fragments arranged by size:
3319 1425 644 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 3522 4352 4711 4870 518
Size: 648 1177 1179 830 359 159 2042
Fragments arranged by size:
2042 1179 1177 830 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3152 3810 4487 5635 5880 1606
Size: 1546 658 677 1148 245 2120
Fragments arranged by size:
2120 1546 1148 677 658 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5842
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 2779 3752 5297 1132
Size: 97 1550 973 1545 2229
Fragments arranged by size:
2229 1550 1545 973 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6394
PciI A'CATG_T
Cuts at: 2775 5293 2775
Size: 2518 3876
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3042 1108
Size: 1934 4460
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4463 4495 5976 6008 4463
Size: 32 1481 32 4849
Fragments arranged by size:
4849 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3167 3167
Size: 6394
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PspOMI G'GGCC_C
Cuts at: 2328 2328
Size: 6394
PstI C_TGCA'G
Cuts at: 830 3746 830
Size: 2916 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
PvuII CAG'CTG
Cuts at: 3152 3152
Size: 6394
SacI G_AGCT'C
Cuts at: 3494 3494
Size: 6394
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SapI GCTCTTCn'nnn_
Cuts at: 3576 3576
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SexAI A'CCwGG_T
Cuts at: 3212 3212
Size: 6394
SfcI C'TryA_G
Cuts at: 826 1045 3742 4877 5558 5749 826
Size: 219 2697 1135 681 191 1471
Fragments arranged by size:
2697 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3721 1872
Size: 1849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2482 4498 5399 5661 820
Size: 197 926 282 257 2016 901 262 1553
Fragments arranged by size:
2016 1553 926 901 282 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3691 4185 493
Size: 3198 494 2702
Fragments arranged by size:
3198 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 2586 4318 5
Size: 47 2534 1732 2081
Fragments arranged by size:
2534 2081 1732 47
StuI AGG'CCT
Cuts at: 1517 3347 3611 1517
Size: 1830 264 4300
Fragments arranged by size:
4300 1830 264
StyI C'CwwG_G
Cuts at: 513 1065 3163 3833 513
Size: 552 2098 670 3074
Fragments arranged by size:
3074 2098 670 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2981 3245 4421 4438 4591 4776 5221
Size: 1255 1401 264 1176 17 153 185 445
Cuts at: 5221 325
Size: 1498
Fragments arranged by size:
1498 1401 1255 1176 445 264 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 4640 6352
Size: 274 80 33 51 1443 642 2021 1712
Cuts at: 6352 96
Size: 138
Fragments arranged by size:
2021 1712 1443 642 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 6354 6386 2543
Size: 32 3779 32 2551
Fragments arranged by size:
3779 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6394
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3247 3247
Size: 6394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3719 1870
Size: 1849 4545
XmnI GAAnn'nnTTC
Cuts at: 4523 4523
Size: 6394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI